Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,051 C→G 5.9% intergenic (+34/‑164) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,0510CG 5.9% 233.3 / 4.5 82intergenic (+34/‑164)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base C (36/41);  new base G (2/3);  total (38/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

ACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGC  >  NC_000913/3780904‑3781200
                                                                                                                                                   |                                                                                                                                                      
aCGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAg                                                                                                                                                      <  1:590716/150‑1 (MQ=255)
aCGCTGACTGGCGCGAAATCGATCAGCGAAAATACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAg                                                                                                                                                      <  2:399568/150‑1 (MQ=255)
    tGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGgag                                                                                                                                                  <  1:756257/150‑1 (MQ=255)
     gACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGgaga                                                                                                                                                 >  1:922828/1‑150 (MQ=255)
      ccTGGCGCGAAAACGATCAGCGAAATTACGCTAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGagag                                                                                                                                               <  2:127687/149‑1 (MQ=255)
        tGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCGGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGGCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAggg                                                                                                                                             <  2:808086/150‑1 (MQ=255)
         ggCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGTTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCCTAGGAGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAgggg                                                                                                                                            >  2:291062/1‑150 (MQ=255)
         ggCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAggcg                                                                                                                                            >  2:22703/1‑148 (MQ=255)
           cgcgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCTAACCCTCTCCCGCAGGGAGAGGGGAc                                                                                                                                          >  2:1243213/1‑150 (MQ=255)
              gAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAgggttaggg                                                                                                                                       <  1:1042834/150‑7 (MQ=255)
               aaaTCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGtt                                                                                                                                      >  1:1140216/1‑150 (MQ=255)
               aaaTCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGtt                                                                                                                                      >  2:102340/1‑150 (MQ=255)
                   cGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCCCCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgc                                                                                                                                  >  2:1110024/1‑150 (MQ=255)
                   cGATCAGCGAAATTACGCAAGATTCGCGGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgc                                                                                                                                  <  1:1127712/150‑1 (MQ=255)
                     aTCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGGCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcgc                                                                                                                                <  1:102340/150‑1 (MQ=255)
                      tCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATTGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCt                                                                                                                               <  1:956623/150‑1 (MQ=255)
                           gAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACCCCCTAACCCTCTCCCTCAGGGCGAGGGGACCGTTCGGCGCtgtatg                                                                                                                          >  2:293336/1‑150 (MQ=255)
                              aTTACGCAAGATTCGCTGGTGCAGCGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTAc                                                                                                                       <  2:935531/150‑1 (MQ=255)
                                  cGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGGGGGGACCGGTCGGCGCTGGATGTACTccc                                                                                                                   >  1:49036/1‑150 (MQ=255)
                                   gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGTGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCg                                                                                                                                    >  1:915669/1‑132 (MQ=255)
                                   gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGTGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCg                                                                                                                                    <  2:915669/132‑1 (MQ=255)
                                   gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCCCCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCAGTATGTACTCCCt                                                                                                                  >  1:1252285/1‑150 (MQ=255)
                                    cAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCACAGGGAGAGGGGACCGGTCGGCGCTGTATGTACTgccgc                                                                                                                 >  2:1651225/1‑148 (MQ=255)
                                      aGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCAc                                                                                                               <  1:547811/150‑1 (MQ=255)
                                          tCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTg                                                                                                           <  1:1633867/150‑1 (MQ=255)
                                          tCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTg                                                                                                           <  2:1349098/150‑1 (MQ=255)
                                           cGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGa                                                                                                          >  2:41033/1‑150 (MQ=255)
                                             cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCACCCTCAGGGAGAGGGGACCGTTCGGCGCGGTATGTACTCCCGCACTCTGaaa                                                                                                        >  2:329874/1‑150 (MQ=255)
                                              tGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGTCGAAAGGGAATGCGGCATAGGCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAAc                                                                                                       <  1:105842/150‑1 (MQ=255)
                                              tGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAAc                                                                                                       <  2:186891/150‑1 (MQ=255)
                                               ggTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACg                                                                                                      <  2:514886/150‑1 (MQ=255)
                                                     ggggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACACCCTCACTCTGCAACGCCACCg                                                                                                >  2:317760/1‑150 (MQ=255)
                                                     ggggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCAAACCCTCACCCTCAGGGAGAGGGGACCGTTAGGCGCTGTATGTACTACCTCACTCTGAAACGACACCg                                                                                                >  2:744662/1‑150 (MQ=255)
                                                     ggggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAAACCTCACCCGCAGGGGGAGGGGGCCGTTCGGGGCTGTATGTACTCCCTCCCTCTTAAACgcccccc                                                                                                >  2:1704495/1‑144 (MQ=255)
                                                             gTAAAGCGGTGCCTGCGGCACTGGCGCCCATGGCGAAAGGGAATGCGGCATAGGCGTGTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttt                                                                                        <  1:1705572/150‑1 (MQ=255)
                                                                aaGAGTTGCCTTCGGCACTGGCTACCATTGCTAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTTAAACGACACCTCACTCtttttt                                                                                     <  1:987900/150‑1 (MQ=255)
                                                                aaGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCtttttt                                                                                     <  2:1001118/150‑1 (MQ=255)
                                                                aaGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCcttttt                                                                                     <  1:1446444/150‑1 (MQ=255)
                                                                 agagTTGCCTGCGGATCTTGCTCCCATTGATAAAGTGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCACCCTCAGGGAGAGGGGACCGTTCGGAGCTGTATGGACTCCCTCACTCTGAAACGACACCGCACTCttttttt                                                                                    <  2:170564/150‑1 (MQ=255)
                                                                   agTTGCCTGCGGCACTTGCTCCCAGGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCATGGAGAGGGGACCTTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtct                                                                                  <  1:975397/150‑1 (MQ=255)
                                                                    gTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtctc                                                                                 <  1:1010026/150‑1 (MQ=255)
                                                                     ttGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTcc                                                                                <  1:18094/150‑1 (MQ=255)
                                                                     ttGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTcc                                                                                <  2:922828/150‑1 (MQ=255)
                                                                       gCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCGCAGGGAGAGGGGACCGTTCGGCGCTGTAAGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCAccca                                                                              >  2:289878/1‑149 (MQ=255)
                                                                         cTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCg                                                                            <  1:1294798/150‑1 (MQ=255)
                                                                          tGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGACGTTTGCGCCCCCTCCCCCTAACCCTCTCCCTCAGGTAGACGGTACCTATCGGCGCTGTATGTACTCCCACACAAATAAACGCCACCGCACTCTTTATTTCTCCCTCGc                                                                           >  1:283824/1‑150 (MQ=255)
                                                                                    gCTCCCATGGCGAAAGTGAATGAGGCATAGTAGTTTGCGCCCCCTCAACCTTACCCTCTCCCTCAGCGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCTCTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgggg                                                                 <  2:280889/150‑1 (MQ=255)
                                                                                    gCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgggg                                                                 <  2:473541/150‑1 (MQ=255)
                                                                                     cTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGga                                                                <  1:249584/150‑1 (MQ=255)
                                                                                       cccATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCTCCCCCTAACCCACTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATTTACTACATCAATATGAAACGACACCGCCCACATTTTTTATACCACGCCCCTCCGGGgaga                                                              >  2:948254/1‑150 (MQ=255)
                                                                                       cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                              <  1:1136973/150‑1 (MQ=255)
                                                                                        ccATGGCGAACGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGagag                                                             <  2:735171/150‑1 (MQ=255)
                                                                                        ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGagag                                                             <  1:620065/150‑1 (MQ=255)
                                                                                          aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTACGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAggg                                                           <  2:1081238/150‑1 (MQ=255)
                                                                                            ggCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGGGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAgggag                                                         <  1:1607845/150‑3 (MQ=255)
                                                                                             gCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCg                                                        <  2:817015/150‑1 (MQ=255)
                                                                                              cGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgg                                                       >  2:190091/1‑150 (MQ=255)
                                                                                              cGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGGTCGGCGCGGGATGTACTCCCGCCCGCGGAAACGCCCCCGCCCGCGTTTTTTCGCCCGCGCCCCGCCGGGGGGGGGGGCgg                                                       >  1:1155639/1‑150 (MQ=255)
                                                                                              cGAAAGGGAATGCGGCATAGGCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgg                                                       <  1:1211960/150‑1 (MQ=255)
                                                                                               gAAAGCGAATGCGGCATAGACGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCCTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCggg                                                      <  2:507334/150‑1 (MQ=255)
                                                                                                 aaGGGAATGCGGCATAGGCGTTTGCGCCCCCTCCCCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGt                                                    <  1:642393/150‑1 (MQ=255)
                                                                                                  agcgAATGCGGCATAGTCGTTGGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTCTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTg                                                   <  2:817707/147‑1 (MQ=255)
                                                                                                  acggAATGCCGCATAGTCGTTTGCCCCCCCACAACCAAAACCACAACCTCAGGGACACGGGACCGTTAGGCGCTGTATGTACTCCCTCACGCTGAAACGACACCGCACTATTTTTTTCTCCCACCCCCCGCCGGGGAGAGGGCCGGGGTg                                                   >  2:692980/3‑150 (MQ=255)
                                                                                                     gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                <  1:771661/150‑1 (MQ=255)
                                                                                                        ttcgtaaTAGTCGTTTGAGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGa                                             <  2:1641994/144‑1 (MQ=255)
                                                                                                        tGCGGCATAGTCGTTTGCGCCCCCACCCCCAAACCCACGACCACAGGGAGAGGGGGCCGTTAGGCGCAGTATGTTCTCCCACACACGGAAACGACCCCCCCCACGTTTTTTATACCACCCCCCAACCGGGAGAGGGGCCGGGTGTggggg                                             >  2:596144/1‑149 (MQ=255)
                                                                                                           ggCATAGGCGTGAGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaaaa                                          <  2:312372/150‑1 (MQ=255)
                                                                                                                    gTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACt                                 >  2:1128062/1‑150 (MQ=255)
                                                                                                                          gcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACCCCGCACTCTTTTTTTCTCCCTCGCCCCGCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGcgccc                           >  2:664110/1‑146 (MQ=255)
                                                                                                                          gcCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTAGCCCGCCCCCGGCGGGGGTGGGGGCGGGGGGGGGGGTAAAAGCCCCCCCTGcgccc                           >  2:13894/1‑146 (MQ=255)
                                                                                                                               cTCACCCTAACCCTCTCCCTCAGGGAGACGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACCCCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGTGGAAAAGGACGCACTGCTCACCAAt                       >  1:663554/1‑150 (MQ=255)
                                                                                                                                   cccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGGTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCCCAGCTCCCGAATTTTCt                  >  1:1646719/1‑150 (MQ=255)
                                                                                                                                   cccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCGCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCt                  >  1:1353249/1‑150 (MQ=255)
                                                                                                                                   cccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTGTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCGCTGCTCACCAATTTTCt                  >  2:133635/1‑150 (MQ=255)
                                                                                                                                    ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCTCTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCAGCAATTTTCTg                 >  1:545359/1‑150 (MQ=255)
                                                                                                                                       aaCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTAACCAATTTTCTGCTa              >  2:118322/1‑150 (MQ=255)
                                                                                                                                         cccTCTCCCTGAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGcc                                                                          <  2:242303/88‑1 (MQ=255)
                                                                                                                                         cccTCTCCCTGAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGcc                                                                          >  1:242303/1‑88 (MQ=255)
                                                                                                                                          ccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc           >  2:1480454/1‑150 (MQ=255)
                                                                                                                                           ctctCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGCAACGCCCCCGCCGTCTTTTTTTCTCCCTCGCCCCGCCGGGGAGAGGGCCCGGCTGAGGGGACAAGGCCGCCGTGCTCTCCTATTTTCTGCTATCCt          >  1:452807/1‑150 (MQ=255)
                                                                                                                                               cccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccc      >  1:156131/1‑150 (MQ=255)
                                                                                                                                                 cTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccccc    >  2:52972/1‑150 (MQ=255)
                                                                                                                                                   cAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGc  >  2:1343818/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
ACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGC  >  NC_000913/3780904‑3781200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: