Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,786,902 G→A 6.2% S22N (AGC→AAC)  envC → activator of AmiB,C murein hydrolases, septal ring factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,786,9020GA 6.2% 297.9 / 6.0 99S22N (AGC→AAC) envCactivator of AmiB,C murein hydrolases, septal ring factor
Reads supporting (aligned to +/- strand):  ref base G (43/48);  new base A (4/2);  total (49/50)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.49e-01

Only 100 of 102 total aligned reads displayed.

TGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCA  >  NC_000913/3786755‑3787049
                                                                                                                                                   |                                                                                                                                                   
tGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAgcg                                                                                                                                                   >  2:214660/1‑150 (MQ=255)
tGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAgcg                                                                                                                                                   >  2:583281/1‑150 (MQ=255)
 ggAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCAAGAGGGGAATGGCGATTAATACAAAGACACGGGACGTGAAACCGCGCAGCTTTGCAATCAGGCCCCTCATCTACGCCCGCCTTCTTAgcgg                                                                                                                                                  >  1:228699/1‑149 (MQ=255)
  gAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTAATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGAAGGTTTGAAATCAGGCCCATCATCTACGCCAGCTTTCTTATCGCt                                                                                                                                                 >  1:1363601/1‑150 (MQ=255)
   aaaTCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTg                                                                                                                                                >  2:1527438/1‑150 (MQ=255)
   aaaTCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTg                                                                                                                                                >  1:1602122/1‑150 (MQ=255)
   aaaTCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTg                                                                                                                                                >  2:380508/1‑150 (MQ=255)
      tCCAGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACCCGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCg                                                                                                                                             <  1:138563/150‑1 (MQ=255)
       cccGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGt                                                                                                                                            <  1:1432879/150‑1 (MQ=255)
        ccGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTa                                                                                                                                           <  1:996801/150‑1 (MQ=255)
         cGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGCTAAGAACGCTGGCGTag                                                                                                                                          <  2:733069/150‑2 (MQ=255)
          gCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCCTGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAACCCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTAtt                                                                                                                                         >  2:260026/1‑150 (MQ=255)
              gagaTGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTAttgttg                                                                                                                                     >  1:419843/1‑150 (MQ=255)
                     cTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGAGCCt                                                                                                                              >  1:1043883/1‑150 (MQ=255)
                          aaGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGGTTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTcg                                                                                                                         >  1:991200/1‑149 (MQ=255)
                          aaGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCCGCGTTGTTAGCGCTGGCGTATTGTTGTGCGCCTTTTcc                                                                                                                         >  2:538836/1‑150 (MQ=255)
                           aGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCCTCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCg                                                                                                                        <  2:1078025/150‑1 (MQ=255)
                            gCCGCTGTTCATCGGGGAATCAGCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGc                                                                                                                       <  1:9778/150‑1 (MQ=255)
                             ccGCTGTTCCTCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCCATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGcc                                                                                                                      >  1:854620/1‑150 (MQ=255)
                             ccGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGcc                                                                                                                      >  1:1728893/1‑150 (MQ=255)
                                      aTCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGAt                                                                                                             <  1:1538745/150‑1 (MQ=255)
                                      aTCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGAt                                                                                                             <  2:805394/150‑1 (MQ=255)
                                      aTCGGGGAATAATCCCTCCCCAGGAGGGGAAAGGCGATTAATCCCATGACCCGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACTCCAGCGGTCTTAGCGCTGGCGTCTTGTTGTGCGCCGTTTCCGCCCACGCGGAt                                                                                                             >  2:1086070/1‑150 (MQ=255)
                                       tCGTGGAATACTCCCTCCCCCTGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGTAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATg                                                                                                            <  1:1666322/150‑1 (MQ=255)
                                       tCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATg                                                                                                            <  1:1623141/150‑1 (MQ=255)
                                         gTGGAATACTCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGCCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAg                                                                                                          <  1:486891/150‑1 (MQ=255)
                                         gTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAg                                                                                                          >  1:189661/1‑150 (MQ=255)
                                           ggAATAATCCCTCCCCCTGAGGGGAAAGGCGAATAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCg                                                                                                        <  1:1563366/150‑1 (MQ=255)
                                           ggAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGCTAAGAACGCTGGCGTagatgat                                                                                                                                     <  2:1529595/121‑7 (MQ=38)
                                           ggAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGCTAAGAACGCTGGCGTagatgat                                                                                                                                     >  1:1529595/1‑115 (MQ=38)
                                             aataatCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCTATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTg                                                                                                      <  1:1231714/150‑1 (MQ=255)
                                               taatCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGAc                                                                                                    >  2:352910/1‑150 (MQ=255)
                                                aatCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGAcc                                                                                                   <  2:885027/150‑1 (MQ=255)
                                                 atCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCa                                                                                                  <  1:1302818/150‑1 (MQ=255)
                                                 atCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCa                                                                                                  >  2:894991/1‑150 (MQ=255)
                                                 atCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGGGAAACCGCGCAGGGTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCa                                                                                                  <  1:791658/150‑1 (MQ=255)
                                                  tccctcccCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCaa                                                                                                 >  2:288915/1‑150 (MQ=255)
                                                  tccctcccCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCaa                                                                                                 >  2:1180122/1‑150 (MQ=255)
                                                  tccctcccCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCaa                                                                                                 >  2:1522040/1‑150 (MQ=255)
                                                     ctcccCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTc                                                                                              <  1:1522040/150‑1 (MQ=255)
                                                     ctcccCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTc                                                                                              <  1:288915/150‑1 (MQ=255)
                                                     ctcccCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTc                                                                                              <  1:352910/150‑1 (MQ=255)
                                                        cccATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCaaa                                                                                           >  1:1408144/1‑150 (MQ=255)
                                                        cccATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCaaa                                                                                           <  1:858278/150‑1 (MQ=255)
                                                        cccATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCaaa                                                                                           <  2:1626588/150‑1 (MQ=255)
                                                         ccATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTACTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCaaaa                                                                                          >  1:358431/1‑149 (MQ=255)
                                                          cctGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATc                                                                                         <  2:1082118/148‑1 (MQ=255)
                                                          cATGAGGGGAAAGGCGATTAAAACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATc                                                                                         <  1:1542724/150‑1 (MQ=255)
                                                            tGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTa                                                                                       <  1:1090204/150‑1 (MQ=255)
                                                               ggggAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTc                                                                                    >  2:1587012/1‑150 (MQ=255)
                                                               ggggAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTGCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTc                                                                                    <  1:287433/150‑1 (MQ=255)
                                                                 ggAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGCCCACCTCAAATCTATTCAg                                                                                  >  1:1477323/1‑150 (MQ=255)
                                                                 ggAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAg                                                                                  <  2:834511/150‑1 (MQ=255)
                                                                  gAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAgg                                                                                 <  2:750148/150‑1 (MQ=255)
                                                                  gAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAgg                                                                                 <  1:1587012/150‑1 (MQ=255)
                                                                      ggCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGa                                                                             <  2:1671569/150‑1 (MQ=255)
                                                                        cGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGCCCAACTCAAATCTATTCAGGCTGACa                                                                           >  1:1565493/1‑150 (MQ=255)
                                                                          aTTAATTCCATGACAAGGGCCGTGAAACCGCGCAGGTTTGCAATCAGTCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTTCGCCTTTTCCGCCCACGCGGATGAGCGTGACAAACTCAAATCTATACAGGCTGACaaa                                                                         >  1:312691/1‑148 (MQ=255)
                                                                          aTTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCGGACATc                                                                         <  1:1129028/150‑1 (MQ=255)
                                                                           ttAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGTCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATcg                                                                        <  1:1024229/150‑1 (MQ=255)
                                                                                 ccATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCaa                                                                  >  2:1508988/1‑150 (MQ=255)
                                                                                  cATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCAATCATCTACGCCTGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGCGGGGGCCCCACTGAAATATGTTACGGCTGACTTCGCCGCaaa                                                                 >  1:1088871/1‑150 (MQ=255)
                                                                                    tGACACTGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATAATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACAACGCCGCAAAag                                                               <  2:921354/150‑1 (MQ=255)
                                                                                    tGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAag                                                               <  1:1731844/150‑1 (MQ=255)
                                                                                    tGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAag                                                               <  1:67417/150‑1 (MQ=255)
                                                                                     gACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCCACTCAAATCTATTCAGGCTGACATCGCCGCAAAaga                                                              >  1:1269826/1‑150 (MQ=255)
                                                                                     gACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAaga                                                              >  1:737029/1‑150 (MQ=255)
                                                                                      acacGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAagag                                                             <  2:1168887/150‑1 (MQ=255)
                                                                                      acacGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAagag                                                             <  1:22016/150‑1 (MQ=255)
                                                                                        acGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCGTCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGAGTGACAAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAgcg                                                           <  2:855089/150‑1 (MQ=255)
                                                                                         cGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGCCCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAgcgc                                                          >  2:721324/1‑150 (MQ=255)
                                                                                           ggCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAgcgcgc                                                        <  1:244810/150‑1 (MQ=255)
                                                                                              cGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCggg                                                     >  1:1315790/1‑149 (MQ=255)
                                                                                              cGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGt                                                     >  1:946078/1‑150 (MQ=255)
                                                                                              cGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGt                                                     >  1:627031/1‑150 (MQ=255)
                                                                                              cGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGt                                                     >  1:643099/1‑150 (MQ=255)
                                                                                                tGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTAc                                                   >  2:764388/1‑150 (MQ=255)
                                                                                                 gAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACg                                                  <  1:380508/150‑1 (MQ=255)
                                                                                                  aaaCCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGc                                                 <  1:764026/150‑1 (MQ=255)
                                                                                                    aCCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCa                                               <  1:1194910/150‑1 (MQ=255)
                                                                                                      cgcgCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCACACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCTCAAAAGAGCGCGCGGTACGCCaaa                                             <  1:931766/150‑1 (MQ=255)
                                                                                                         gcAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGc                                          <  1:1527438/150‑1 (MQ=255)
                                                                                                          cAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGca                                         >  1:1306518/1‑150 (MQ=255)
                                                                                                            ggTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGcaac                                       >  1:1413748/1‑150 (MQ=255)
                                                                                                              tttGCAATCAGGCCCATCATCTACGCCAGCGGTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTCCGCCTAAAGcaacaa                                     >  1:919008/1‑150 (MQ=255)
                                                                                                               ttGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGcaacaac                                    <  1:1503527/150‑1 (MQ=255)
                                                                                                                    aTCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAAcgc                               <  1:214660/150‑1 (MQ=255)
                                                                                                                          cccATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGCGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGc                         >  1:1103221/1‑150 (MQ=255)
                                                                                                                                caatACGCCAGCGCTAAGAACGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATACAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTc                   >  2:1500425/4‑150 (MQ=255)
                                                                                                                                 tcTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCg                  <  1:1509548/150‑1 (MQ=255)
                                                                                                                                 tcTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCg                  <  2:643099/150‑1 (MQ=255)
                                                                                                                                 aatACGCCAGCGCTAAGAACGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGCGCGCGCGGTACGCCAAAAGCGACAACAACGCGCAAGCGTGCTCg                  >  1:307529/3‑150 (MQ=255)
                                                                                                                                  atACGCCAGCGCTAAGACCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTTGc                 >  2:335622/2‑150 (MQ=255)
                                                                                                                                  atACGCCAGCGCTAAGAACGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGc                 >  2:217626/2‑150 (MQ=255)
                                                                                                                                       ccAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAAt            >  1:1601060/1‑150 (MQ=255)
                                                                                                                                         aGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGATCGCACAATTg          <  2:1613545/150‑1 (MQ=255)
                                                                                                                                          gCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGa         >  1:988166/1‑150 (MQ=255)
                                                                                                                                             ttCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGaaaa      <  1:1418739/150‑1 (MQ=255)
                                                                                                                                             ttCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGAACGCAAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTCaaaa      >  2:1715520/1‑150 (MQ=255)
                                                                                                                                                 tAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTACTCGCACAATTGAAAAAGCa  >  1:107665/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCA  >  NC_000913/3786755‑3787049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: