Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,796,006 T→C 8.8% S10P (TCG→CCG) ‡ waaC → ADP‑heptose:LPS heptosyl transferase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,796,0060TC 8.8% 314.5 / 17.7 114S10P (TCG→CCG) ‡waaCADP‑heptose:LPS heptosyl transferase I
Reads supporting (aligned to +/- strand):  ref base T (51/53);  new base C (7/3);  total (58/56)
Fisher's exact test for biased strand distribution p-value = 3.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.98e-01

Only 100 of 118 total aligned reads displayed.

CTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAAT  >  NC_000913/3795858‑3796154
                                                                                                                                                    |                                                                                                                                                    
cTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACAtcgtc                                                                                                                                                     <  2:1363875/150‑1 (MQ=255)
cTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACAtcgtc                                                                                                                                                     <  2:333654/150‑1 (MQ=255)
  aTCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACAtcgtcgg                                                                                                                                                   >  1:1427836/1‑149 (MQ=255)
   tCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGAt                                                                                                                                                  >  1:1144106/1‑150 (MQ=255)
   tCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGAt                                                                                                                                                  >  1:1229428/1‑150 (MQ=255)
    cacaAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATg                                                                                                                                                 >  2:1312916/1‑150 (MQ=255)
     acaAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGAtcg                                                                                                                                                >  2:1456810/1‑148 (MQ=255)
     acaAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATgg                                                                                                                                                >  2:1410077/1‑150 (MQ=255)
      caAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATggg                                                                                                                                               <  2:621206/150‑1 (MQ=255)
      caAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATggg                                                                                                                                               <  1:1421647/150‑1 (MQ=255)
        aaGTGCGTAACGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCg                                                                                                                                             <  2:1306587/150‑1 (MQ=255)
        aaGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCg                                                                                                                                             <  2:1346661/150‑1 (MQ=255)
          gTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGAt                                                                                                                                           >  1:1228957/1‑150 (MQ=255)
            gCGTAAAGGGGACGCTGCGGAGGGTTATCACCAGAGCTTACTCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAGAACATCGCCCATCGACGATGt                                                                                                                                         <  2:1283421/150‑1 (MQ=255)
              gTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGTCGATGTtc                                                                                                                                       >  1:691112/1‑150 (MQ=255)
                 aaGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACTGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATcc                                                                                                                                    >  2:1378414/1‑150 (MQ=255)
                  aGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGCCCATCGACGATGttttaac                                                                                                                                   <  2:1307702/150‑6 (MQ=255)
                  aGGTGACGCTGCGGAGGGGTATAACCAGAGTTTAATCGACATTACTCCCCAGCGCGTACTGGAACAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCa                                                                                                                                   <  1:477271/150‑1 (MQ=255)
                   ggTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCAt                                                                                                                                  >  2:803668/1‑150 (MQ=255)
                   ggTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCAt                                                                                                                                  >  2:1022593/1‑150 (MQ=255)
                   ggTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCAt                                                                                                                                  >  1:531385/1‑150 (MQ=255)
                    gTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATa                                                                                                                                 <  1:1504840/150‑1 (MQ=255)
                    gTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATa                                                                                                                                 <  2:1566712/150‑1 (MQ=255)
                      caCGCGGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCTATGGGCGATGTTCTCCATACg                                                                                                                               <  1:1625619/149‑1 (MQ=255)
                        cGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGtt                                                                                                                             <  1:1107135/150‑1 (MQ=255)
                           tGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGcc                                                                                                                          <  1:1312916/150‑1 (MQ=255)
                            gCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGccc                                                                                                                         >  1:607179/1‑150 (MQ=255)
                            gCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGccc                                                                                                                         >  2:706477/1‑150 (MQ=255)
                                 gggTTATCACCAGAGCTTAATCGACCTTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGcact                                                                                                                    <  2:1311089/150‑1 (MQ=255)
                                   gTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGcactca                                                                                                                  >  2:1068969/1‑150 (MQ=255)
                                     tATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGcactcact                                                                                                                <  1:938543/150‑1 (MQ=255)
                                     tATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGcactcact                                                                                                                <  2:1437701/150‑1 (MQ=255)
                                     tATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCCCCATACGTTGCCCGcactcact                                                                                                                <  1:1406152/150‑1 (MQ=255)
                                      aTCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTg                                                                                                               <  2:1597615/150‑1 (MQ=255)
                                      aTCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGGTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTg                                                                                                               <  2:1040487/150‑1 (MQ=255)
                                         cccatcgCTTAATCGAAATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATg                                                                                                            <  1:545405/144‑1 (MQ=255)
                                          ccAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGc                                                                                                           >  2:1207682/1‑150 (MQ=255)
                                              agCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCcag                                                                                                       <  2:942776/150‑1 (MQ=255)
                                               gCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCcagc                                                                                                      >  1:1422088/1‑150 (MQ=255)
                                               gCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCcagc                                                                                                      >  2:1163498/1‑150 (MQ=255)
                                                cTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCcagca                                                                                                     >  2:1178351/1‑150 (MQ=255)
                                                 ttAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCcagcag                                                                                                    <  2:888088/150‑1 (MQ=255)
                                                   aaTCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGc                                                                                                  >  2:1513506/1‑150 (MQ=255)
                                                    aTCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCa                                                                                                 >  1:1017791/1‑150 (MQ=255)
                                                      cGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAAt                                                                                               >  2:217418/1‑150 (MQ=255)
                                                          aTTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCa                                                                                           <  2:840573/150‑1 (MQ=255)
                                                            tACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAAAATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAgg                                                                                         <  1:1502664/150‑1 (MQ=255)
                                                             aCTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAggg                                                                                        <  2:630993/150‑1 (MQ=255)
                                                                ccccAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGAtt                                                                                     >  1:170311/1‑150 (MQ=255)
                                                                   cAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAg                                                                                  <  1:498189/150‑1 (MQ=255)
                                                                      cgcgTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGttt                                                                               >  2:500139/1‑150 (MQ=255)
                                                                        cgTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGa                                                                             >  1:551780/1‑150 (MQ=255)
                                                                        cgTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGa                                                                             >  1:558726/1‑150 (MQ=255)
                                                                         gTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGAc                                                                            <  2:1459412/150‑1 (MQ=255)
                                                                          tACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACt                                                                           <  1:995779/150‑1 (MQ=255)
                                                                               gaataaCACAACGCGCTATTGGTACAAGCCGAAGCCTGACGGATGCGGGGGTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGggtg                                                                      <  2:593415/150‑1 (MQ=255)
                                                                               gaagaaCTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGggtg                                                                      <  2:1577060/150‑1 (MQ=255)
                                                                               gaagaaCTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGggtg                                                                      <  1:682981/150‑1 (MQ=255)
                                                                                  gaaCTCCACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGgtggtg                                                                   <  1:1068969/150‑1 (MQ=255)
                                                                                   aaCTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGtggtgg                                                                  <  2:1144106/150‑1 (MQ=255)
                                                                                    aCTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGga                                                                 >  1:584835/1‑150 (MQ=255)
                                                                                     cTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGAAGGCAATCCCAGGGATTAAGATTGACTGGGTGGTGgaa                                                                >  2:541480/1‑150 (MQ=255)
                                                                                      tCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGgaag                                                               <  2:807819/150‑1 (MQ=255)
                                                                                       cAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGgaaga                                                              >  2:1584750/1‑150 (MQ=255)
                                                                                        aaCGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGgaagaa                                                             >  2:1698335/1‑150 (MQ=255)
                                                                                         aCGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGaagaag                                                            <  1:1163498/150‑1 (MQ=255)
                                                                                          cgcgCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAgg                                                           <  1:1022593/150‑1 (MQ=255)
                                                                                             gcTATTGTTACAAGTGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAAGATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGtt                                                        <  1:76574/150‑1 (MQ=255)
                                                                                                aTTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGc                                                     >  1:187732/1‑150 (MQ=255)
                                                                                                    ttACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAg                                                 >  2:1442033/1‑150 (MQ=255)
                                                                                                     tACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGCCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGCATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGa                                                >  1:1592228/1‑150 (MQ=255)
                                                                                                     tACAAGAGGAAGCCTGACGGATGCGGGGTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATG‑CCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAt                                               <  1:737985/150‑1 (MQ=255)
                                                                                                      aCAAGAGGAAGCCTGACGGTTGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCTCACAGAt                                               <  2:170311/150‑1 (MQ=255)
                                                                                                        aaGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAttc                                             <  1:369895/150‑1 (MQ=255)
                                                                                                            ggAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAttccttc                                         >  2:938874/1‑150 (MQ=255)
                                                                                                             gAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAttccttcc                                        <  1:217418/150‑1 (MQ=255)
                                                                                                             gAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAttccttcc                                        >  1:200962/1‑150 (MQ=255)
                                                                                                               aGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTg                                      >  2:6574/1‑150 (MQ=255)
                                                                                                                gCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGTTGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTgg                                     <  1:1349018/150‑1 (MQ=255)
                                                                                                                  cTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGcac                                                   <  1:480849/134‑1 (MQ=255)
                                                                                                                  cTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGcac                                                   >  2:480849/1‑134 (MQ=255)
                                                                                                                  cTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCa                                   >  1:518890/1‑150 (MQ=255)
                                                                                                                    gACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACg                                 <  2:1229428/150‑1 (MQ=255)
                                                                                                                          ggCGGGTTTTGATCGTTAAACCATCGTCGAGGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGCTTGACTGGGGGGGGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCg                           <  2:1391077/149‑1 (MQ=255)
                                                                                                                                    gATCGTTAAAACATCGTTGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGt                 >  1:994574/1‑150 (MQ=255)
                                                                                                                                    gATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGt                 <  2:218470/150‑1 (MQ=255)
                                                                                                                                    gATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGt                 >  1:376206/1‑150 (MQ=255)
                                                                                                                                       cGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTAt              >  1:1683301/1‑150 (MQ=255)
                                                                                                                                       cGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTAt              >  2:1418075/1‑150 (MQ=255)
                                                                                                                                        gTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTAtt             <  1:1410077/150‑1 (MQ=255)
                                                                                                                                         ggagaaCATCGCCCATCGACGATGTTTTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTc            >  2:496515/5‑150 (MQ=255)
                                                                                                                                         ggagaaCATCGCCCATCGACGATGTTTTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTACGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTAtta            >  1:849849/5‑149 (MQ=255)
                                                                                                                                         ggagaaCATCGCCCATCGACGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTc            >  1:13559/5‑150 (MQ=255)
                                                                                                                                         ggagaaCATCGCCCATCGACGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTc            >  1:1274658/5‑150 (MQ=255)
                                                                                                                                          tAAAACATCGTCGATGGGGGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTcc           <  2:691112/150‑1 (MQ=255)
                                                                                                                                          tAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTcc           <  2:1422088/150‑1 (MQ=255)
                                                                                                                                          gagaaCATCGCCCATCGACGATGTTTTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTcc           >  1:480713/4‑150 (MQ=255)
                                                                                                                                              aCATCGCCCATCGACGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCtgtg       >  1:65125/1‑150 (MQ=255)
                                                                                                                                               cATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCGCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGGTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTgg      <  2:1137703/150‑1 (MQ=255)
                                                                                                                                                   gtcgATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAAt  <  2:200962/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAAT  >  NC_000913/3795858‑3796154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑