Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,815,816 C→G 6.6% intergenic (‑48/+18) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,815,8160CG 6.6% 327.2 / 9.9 102intergenic (‑48/+18)pyrE/rphorotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
Reads supporting (aligned to +/- strand):  ref base C (51/44);  new base G (4/3);  total (55/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

Only 100 of 104 total aligned reads displayed.

AAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGC  >  NC_000913/3815668‑3815963
                                                                                                                                                     |                                                                                                                                                   
aattAGGGGCTTTTGCGCCCGTATTTAAGCTTAAACTCGCAAAAATTAAACACATGCTTGCTAAGCTCAAATTCAATAAACTGGCGCTGATTTGGTTTATTGCCTTCGCTCNTAATCTTACTTTTCAACACAAAAAAAAAACGCGACtca                                                                                                                                                    <  2:1668458/147‑1 (MQ=255)
   tAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACtcatca                                                                                                                                                 >  1:1715622/1‑150 (MQ=255)
    aGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAg                                                                                                                                                >  2:206171/1‑150 (MQ=255)
       ggCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCg                                                                                                                                             <  1:1719232/150‑1 (MQ=255)
       ggCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCg                                                                                                                                             >  1:1372578/1‑150 (MQ=255)
         gtttgGCGCCGGTATTTCAGAGTAAACGCTCCAAAATTTAACACTTGCTTGCTAAGCGCAAATTCAATAAAATGGCGCTGATATGTTTTCTTGCTTTCGCTCCTCATGTTACTTTTCTACAGACAAAAAAACGGCGACTCATCAGTCGcc                                                                                                                                           <  2:1551819/149‑1 (MQ=255)
         cTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCTAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCTTCAGTCGcc                                                                                                                                           >  1:1398023/1‑150 (MQ=255)
           tttGGGCCCGTATTTCTGCGAAAACTCTCAAAAATTTAACACCTGCTTGCTAAGCTAAAATTCAATAAACTGGCGATGATTTGGTTTCATGCCTTCGCTCCACATATTACTTTACTACAGACAAAAAAACGGCGACTCATCAGTCGCCtt                                                                                                                                         <  2:647651/150‑1 (MQ=255)
           tttGCGCCGGTATTTCAGCGTAAACTCGCAAAATTTAAACACCTGCTTGCAACGCGAAAATTCAAAAAACTGGCGCTTATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTAAACAGAAAAAAAAAACGCGACTCATCAGTCGCCtt                                                                                                                                         <  2:702621/150‑1 (MQ=255)
           tttGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGAAATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCAACAGACAAAAAAAAGGCGACTCATCAGTCGCCtt                                                                                                                                         <  2:1410741/150‑1 (MQ=255)
           ttggcgcCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCAAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTGATGAGTCGCCtt                                                                                                                                         <  2:616709/147‑1 (MQ=255)
             tGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTGATGAGTCGCCtttt                                                                                                                                       <  2:1174786/150‑3 (MQ=255)
              gcgcCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTaaa                                                                                                                                      >  1:852292/1‑150 (MQ=255)
                gcCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTaaaaa                                                                                                                                    >  1:415914/1‑150 (MQ=255)
                gcCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTaaaaa                                                                                                                                    >  1:455769/1‑150 (MQ=255)
                 cccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATATTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAAt                                                                                                                                   >  2:201754/1‑150 (MQ=255)
                  ccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTGATGAGTCGCCttttttttg                                                                                                                                  <  2:86502/150‑8 (MQ=255)
                  ccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATc                                                                                                                                  >  2:1585296/1‑150 (MQ=255)
                    ggATTTCAGCGTAAACTCCCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCGGATATGGTTGCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAg                                                                                                                                <  1:1073164/150‑1 (MQ=255)
                            gCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAg                                                                                                                        <  2:1213270/150‑1 (MQ=255)
                            gCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACAGGCGCTGATATGGTTTCATTCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCTACTCATCAGTCGCCTTAAAATTCAGATTGCCaa                                                                                                                        >  1:7644/1‑149 (MQ=255)
                              gTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAgcg                                                                                                                      >  2:1386967/1‑150 (MQ=255)
                              gTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAgcg                                                                                                                      >  1:36971/1‑150 (MQ=255)
                                aaaCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAAAAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTCCAGACAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgcc                                                                                                                    <  1:557894/150‑1 (MQ=255)
                                 aaCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgccg                                                                                                                   <  1:113071/150‑1 (MQ=255)
                                 aaCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCAGCAGTCGCCTTAAAAATCAGATTGCCAGcgccc                                                                                                                   >  1:1395427/1‑149 (MQ=255)
                                 aaCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCATcgccg                                                                                                                   >  2:882422/1‑150 (MQ=255)
                                  aCTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGcgccgc                                                                                                                  >  2:1249641/1‑150 (MQ=255)
                                   cTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTAATTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCgccgcc                                                                                                                 <  2:1615676/150‑1 (MQ=255)
                                   atCGCCAAACTTTAACACCTGCTTGATAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTAATTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCgccgcc                                                                                                                 <  2:1466812/149‑1 (MQ=255)
                                        cAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTg                                                                                                            <  2:503943/150‑1 (MQ=255)
                                         aaaCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGATTCATTCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCAGc                                                                                                           >  1:1256170/1‑150 (MQ=255)
                                                 acacCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTAc                                                                                                   >  2:138042/1‑150 (MQ=255)
                                                 acacCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTAACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTAc                                                                                                   <  1:937481/150‑1 (MQ=255)
                                                  cacCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACa                                                                                                  >  2:1667010/1‑150 (MQ=255)
                                                     cTGCTTGCTAAGCGCAAATTCAATAACCTGGCGCTTATATGGTTTCATGCCTCCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATg                                                                                               <  1:1610236/150‑1 (MQ=255)
                                                          tgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAATCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattc                                                                                          >  2:608393/1‑150 (MQ=255)
                                                          tgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCATACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattc                                                                                          <  1:888401/150‑1 (MQ=255)
                                                           gctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcg                                                                                         >  1:39975/1‑150 (MQ=255)
                                                           gctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcg                                                                                         <  2:659683/150‑1 (MQ=255)
                                                           gctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcg                                                                                         >  2:731156/1‑150 (MQ=255)
                                                              aaGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcgatt                                                                                      >  2:79006/1‑150 (MQ=255)
                                                               aGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcgattc                                                                                     >  2:349913/1‑150 (MQ=255)
                                                               aGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcgattc                                                                                     >  1:909021/1‑150 (MQ=255)
                                                                  ccAAATTCAATAAACTGCGGCTGATATGGTTTCATGCCTTCGCTCCTCTTCTAAGTTTTCTACAGACAAAAAACAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTcccc                                                                                  <  1:181302/149‑1 (MQ=255)
                                                                       ttaaaTAAACTGGGTCTTATATGGTTTCATGCCTTCGATCCTCATTTTACTTTTCAACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCggg                                                                             <  2:1529334/147‑1 (MQ=255)
                                                                       ttCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCggg                                                                             <  2:452293/150‑1 (MQ=255)
                                                                         cAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGcc                                                                           >  2:1587426/1‑150 (MQ=255)
                                                                         aaaTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGcc                                                                           <  2:1630464/149‑1 (MQ=255)
                                                                          aaTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCa                                                                          >  2:1463817/1‑150 (MQ=255)
                                                                             aaaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCagag                                                                       >  1:707746/1‑150 (MQ=255)
                                                                                cTGGCGCTGAAATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCca                                                                    <  1:1416249/150‑1 (MQ=255)
                                                                                  ggCGCTTATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACATACAAAAAAAACGAGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCcaac                                                                  <  2:850786/150‑1 (MQ=255)
                                                                                  ggCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAAGGCGCCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCcaa                                                                   >  2:1535172/1‑150 (MQ=255)
                                                                                    cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATAAGTTTGCCAGCGCCGCCTACTGCGTCGCTACAATTGATTCGATTCCCCTCGGGCCAGAGCcaacaa                                                                >  2:881960/1‑150 (MQ=255)
                                                                                     gcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAAAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAg                                                               <  2:852292/150‑1 (MQ=255)
                                                                                        gATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCCGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATg                                                            <  2:912664/150‑1 (MQ=255)
                                                                                        gATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATg                                                            <  1:1240073/150‑1 (MQ=255)
                                                                                        gATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATg                                                            >  2:637003/1‑150 (MQ=255)
                                                                                        gATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAAGGGATTCGATTCCCCTCGGGCCAGCGACAACAAGATg                                                            >  1:318955/1‑150 (MQ=255)
                                                                                          tatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAg                                                          >  1:100545/1‑150 (MQ=255)
                                                                                          tatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAg                                                          >  2:830326/1‑150 (MQ=255)
                                                                                           atGGTTTCATGCCTTCGCTCCTCATCTTACTTTTATACATACAAAAAAACGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGt                                                         <  1:397944/150‑1 (MQ=255)
                                                                                            tGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTa                                                        <  1:964891/150‑1 (MQ=255)
                                                                                              gTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAAGGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGc                                                      >  2:1419996/1‑150 (MQ=255)
                                                                                                  cATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCtt                                                  >  2:1352128/1‑150 (MQ=255)
                                                                                                  cATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCtt                                                  >  1:623615/1‑150 (MQ=255)
                                                                                                  cATGCCTTCGCACATCATCTAACTTTTCAACACAAAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCtt                                                  <  1:1076840/150‑1 (MQ=255)
                                                                                                   aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGCTTCGATTCCCCTCGGGCCCGAGCCAACATGATGCGTAGctcgtg                                                 >  1:1116264/1‑147 (MQ=255)
                                                                                                      ccTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATg                                              <  2:1580356/150‑1 (MQ=255)
                                                                                                      ccTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCCCCTTATGCGTCGCTACAATGGAATCCATTCCACTCGGGCCAGCGCCAAGAAGATGAGTAGCTCATCATg                                              >  1:174392/1‑150 (MQ=255)
                                                                                                        ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATggg                                            <  1:525390/150‑1 (MQ=255)
                                                                                                          cGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTg                                          <  2:909160/150‑1 (MQ=255)
                                                                                                             ccctcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAAc                                       <  1:1567737/149‑1 (MQ=255)
                                                                                                              cctcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACg                                      >  1:872558/1‑150 (MQ=255)
                                                                                                                 cATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCt                                   >  2:357670/1‑150 (MQ=255)
                                                                                                                        cTTTTCTACATACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTc                            <  1:1413496/150‑1 (MQ=255)
                                                                                                                        cTTTACTACAGACAAAAAAACGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTc                            <  1:354418/150‑1 (MQ=255)
                                                                                                                          tttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTg                          >  1:211155/1‑150 (MQ=255)
                                                                                                                           ttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGc                         <  1:206171/150‑1 (MQ=255)
                                                                                                                               aCAGACAAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGt                      >  2:891829/1‑150 (MQ=255)
                                                                                                                               aCAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTc                     >  2:1245520/1‑150 (MQ=255)
                                                                                                                               aCAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTc                     >  1:138341/1‑150 (MQ=255)
                                                                                                                                cAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcc                    <  2:415914/150‑1 (MQ=255)
                                                                                                                                cAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcc                    >  2:1300022/1‑150 (MQ=255)
                                                                                                                                cAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCGACAATGGATTCGATTCCCCTCGGGCCAGCGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGACTCGCCTTCTGCCGTcc                    >  1:1300899/1‑150 (MQ=255)
                                                                                                                                 cgACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTccc                   <  1:767967/149‑1 (MQ=255)
                                                                                                                                  gACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTGCAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc                  >  1:562308/1‑150 (MQ=255)
                                                                                                                                  gACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGAGTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc                  <  2:6564/150‑1 (MQ=255)
                                                                                                                                      aaaaaataGGCGAATCATCAGTCGCGTTAAAATTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGTGTAGCTGTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGc               <  2:7644/150‑1 (MQ=255)
                                                                                                                                      aaaaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGc               <  1:769018/150‑1 (MQ=255)
                                                                                                                                      aaaaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGc               >  1:1613554/1‑150 (MQ=255)
                                                                                                                                      aaaaaaaaGGCGACGCATCAGTCGCCTTAAAAATCAGTTGGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGc               <  1:133425/150‑1 (MQ=255)
                                                                                                                                        aaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCAc             >  2:200951/1‑150 (MQ=255)
                                                                                                                                          ttaaGGCGACTGATGAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGCGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACtt           >  1:183813/3‑150 (MQ=255)
                                                                                                                                           taaGGCGACTGATGAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTc          >  2:1263023/2‑150 (MQ=255)
                                                                                                                                           aaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTc          <  2:138341/150‑1 (MQ=255)
                                                                                                                                            aaGGCGACTGATGAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCa         >  1:1386542/1‑150 (MQ=255)
                                                                                                                                             aGGCGACTGATGAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCaa        >  2:388531/1‑150 (MQ=255)
                                                                                                                                                   cTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgc  >  1:1333207/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
AAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGC  >  NC_000913/3815668‑3815963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: