Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 493,335 C→G 9.3% A415G (GCG→GGG) ‡ dnaX → DNA polymerase III/DNA elongation factor III, tau and gamma subunits

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913493,3350CG 9.3% 274.0 / 15.8 86A415G (GCG→GGG) ‡dnaXDNA polymerase III/DNA elongation factor III, tau and gamma subunits
Reads supporting (aligned to +/- strand):  ref base C (42/36);  new base G (5/3);  total (47/39)
Fisher's exact test for biased strand distribution p-value = 7.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

GCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTCCGGTGCCATCGG  >  NC_000913/493186‑493484
                                                                                                                                                     |                                                                                                                                                     
gCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCACCCCAGGTGCCGCCGCAACCGCAATCCGCGCCGCCGCAGGCACCGACTGTACCGCTCCCGGACCCCCCCAGCCAGGTGCTggcggc                                                                                                                                                       >  2:898457/1‑150 (MQ=255)
gCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCCATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCCCCAGCCCGGTGCTggcggc                                                                                                                                                       >  2:942152/1‑150 (MQ=255)
  cTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGCCTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGgcgc                                                                                                                                                     >  1:777355/1‑150 (MQ=255)
   tGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGgcgcg                                                                                                                                                    >  2:1174887/1‑150 (MQ=255)
      gCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGCCGCCAACCCAGGTGCCGCCGCACCCGCAATCAGCGCCGCAGCCGGCACCGCCTGTACCGCTCCCGGAAACCCCCCGCCAGGTGCTGGCGGCGCGccc                                                                                                                                                 >  2:52265/1‑149 (MQ=255)
      gCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGccc                                                                                                                                                 >  1:483518/1‑149 (MQ=255)
       ccAGAAGTGCCACGACAGTCCTTTGCTCCCGTCGCGCCAACGGCAGTAATGCCGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCCCCAGCCAGGTGCTGGCGGCGCGCcag                                                                                                                                                >  2:501811/1‑150 (MQ=255)
       ccAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCcag                                                                                                                                                >  2:1186088/1‑150 (MQ=255)
        cAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGCTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCAAGGTGCTGGCGCGCCGCcagc                                                                                                                                               <  2:1209759/150‑1 (MQ=255)
         aGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCcagca                                                                                                                                              >  2:1730737/1‑150 (MQ=255)
         aGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGCGCCGCcagca                                                                                                                                              <  2:504296/150‑1 (MQ=255)
           acgTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGCGCCGCcagcacc                                                                                                                                            <  2:1176573/148‑3 (MQ=255)
              tGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGc                                                                                                                                         <  2:1516177/150‑1 (MQ=255)
              tGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGc                                                                                                                                         <  2:1362569/150‑1 (MQ=255)
               gCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCACCCGCAATCAGCGCCGCAGCAGGCACCGCCTGTACCGCTCCCGGAACCCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCa                                                                                                                                        >  2:949435/1‑150 (MQ=255)
               gCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCa                                                                                                                                        >  2:1386678/1‑150 (MQ=255)
                ccccGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAg                                                                                                                                       >  2:1186929/4‑150 (MQ=255)
                ccACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCACCCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAg                                                                                                                                       >  2:749456/1‑150 (MQ=255)
                 cACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGCCGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCCGCCAGGTGCTGGCGGCGCGCCCGCAGTTGCAgc                                                                                                                                      >  2:1049589/1‑150 (MQ=255)
                     aCAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGGGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCCGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGt                                                                                                                                  <  1:790245/150‑1 (MQ=255)
                          ccTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGACACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAgg                                                                                                                             >  1:502855/1‑150 (MQ=255)
                               gCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCa                                                                                                                        <  1:600720/150‑1 (MQ=255)
                                  cccGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAAcc                                                                                                                     <  2:1431094/150‑1 (MQ=255)
                                  cccGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGAGTGCAGGGAGCAAcc                                                                                                                     >  2:1274904/1‑150 (MQ=255)
                                     gTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGCAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCcaa                                                                                                                  >  2:870249/1‑150 (MQ=255)
                                        gcgcCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCa                                                                                                               <  1:1386678/150‑1 (MQ=255)
                                          gcCAACGGCAGTAATGACGCCAACCCCGGTGCCGCCGCAACCGCAATCCGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCCGCGCGGGCGGGGAGCACCCAAAGCaaa                                                                                                             >  2:727702/1‑150 (MQ=255)
                                          gcCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCTGGGAGCAACCAAAGCaaa                                                                                                             <  2:1709596/150‑1 (MQ=255)
                                           ccAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCaaaa                                                                                                            >  2:1495628/1‑150 (MQ=255)
                                              aCGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAag                                                                                                         <  2:1518177/150‑1 (MQ=255)
                                               cGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAaga                                                                                                        >  1:1727643/1‑150 (MQ=255)
                                                  cAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTg                                                                                                     <  2:599561/150‑1 (MQ=255)
                                                    gTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGCGTGaa                                                                                                   >  1:1267916/1‑150 (MQ=255)
                                                     tAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAAc                                                                                                  <  1:179809/150‑1 (MQ=255)
                                                       aTGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCg                                                                                                <  1:352107/150‑1 (MQ=255)
                                                        tGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCgg                                                                                               >  2:1340377/1‑150 (MQ=255)
                                                        tGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCgg                                                                                               <  2:892022/150‑1 (MQ=255)
                                                              cAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCg                                                                                         >  2:1653252/1‑150 (MQ=255)
                                                               aaCCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCATCAGCCAGGTGCTGGCGGCGCGCGAGCAGTTGCAGCGCGTGCAGTGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGc                                                                                        <  1:295129/150‑1 (MQ=255)
                                                                aCCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCt                                                                                       <  1:1160240/150‑1 (MQ=255)
                                                                     ggTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCcg                                                                                  >  1:1120282/1‑150 (MQ=255)
                                                                       ggccgccGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCcgcg                                                                                <  2:1408302/149‑1 (MQ=255)
                                                                           gccgcAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCcgcgcgcg                                                                            >  1:1336029/1‑150 (MQ=255)
                                                                                 accgcaaTCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCTCCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCTGCAGCCGCTACCCGCGCGCGGCCGGt                                                                      <  1:1454186/150‑1 (MQ=255)
                                                                                  ccgcaaTCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCCCCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTg                                                                     <  1:187894/150‑1 (MQ=255)
                                                                                  ccgcaaTCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCCGCCGCTACCCGCGCGCGGCCGGTg                                                                     >  2:1637602/1‑150 (MQ=255)
                                                                                  ccgcaaTCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTg                                                                     <  2:742917/150‑1 (MQ=255)
                                                                                  ccgcaaTCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGACAGCCGCTACCCGCGCGCGGCCGGTg                                                                     >  1:576142/1‑150 (MQ=255)
                                                                                    gcaaTCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGaa                                                                   >  1:768850/1‑150 (MQ=255)
                                                                                     caaTCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAAt                                                                  >  1:127299/1‑150 (MQ=255)
                                                                                           gcgcCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCATGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAAcgcg                                                            >  2:472737/1‑149 (MQ=255)
                                                                                             gccgcAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCAGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGATCCGGCAGCCGCTACCCGCGCGCGGCCGGTAATTAAcgctgc                                                          <  2:759426/150‑1 (MQ=255)
                                                                                                gcagcaGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAAcgctgcgct                                                       >  2:754913/1‑150 (MQ=255)
                                                                                                 cagcagGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGTGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAAcgctgcgctg                                                      <  2:300874/150‑1 (MQ=255)
                                                                                                 cagcagGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAAcgctgcgctg                                                      <  2:1728343/150‑1 (MQ=255)
                                                                                                  agcagGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTgg                                                     <  2:1257624/150‑1 (MQ=255)
                                                                                                      ggCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGTATAACGCTGCGCTGGAAAg                                                 >  2:1230733/1‑150 (MQ=255)
                                                                                                         aCCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACt                                              <  1:552607/150‑1 (MQ=255)
                                                                                                           cGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTgg                                            <  2:1202032/150‑1 (MQ=255)
                                                                                                            gACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGc                                           >  1:448292/1‑150 (MQ=255)
                                                                                                            gACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGc                                           <  2:257192/150‑1 (MQ=255)
                                                                                                             aCTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCt                                          <  1:1730737/150‑1 (MQ=255)
                                                                                                                gTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCg                                       <  2:547443/150‑1 (MQ=255)
                                                                                                                 tACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGCGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCgg                                      >  1:329024/1‑150 (MQ=255)
                                                                                                                   ccGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTc                                    <  1:1174887/150‑1 (MQ=255)
                                                                                                                      cTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGCCTGGCTTCGGTCAcc                                 >  1:334095/1‑150 (MQ=255)
                                                                                                                        cccGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCGGGAAAGACTGGCTTCGGTCACCGa                               >  1:1082208/1‑150 (MQ=255)
                                                                                                                         ccGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGAt                              >  2:147597/1‑150 (MQ=255)
                                                                                                                          cGGAAACCACCAGCCCGGTGCGGGCGGCGCGCCAGCAGTGGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAACGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATc                             <  1:870249/150‑1 (MQ=255)
                                                                                                                            gAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCACGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATcgc                           <  1:1025292/150‑1 (MQ=255)
                                                                                                                               accaccAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGATTAACGCTGCGCTGGATAGACTGGCTTCGGTCACCGATCGCGtt                        >  1:1574775/1‑150 (MQ=255)
                                                                                                                                ccaccaGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTc                       >  2:1724186/1‑150 (MQ=255)
                                                                                                                                ccaccaGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTc                       >  2:1392719/1‑150 (MQ=255)
                                                                                                                                  accaGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCACCCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGGGAATAACGCTGCGCGGGAACGCCTGGCTGCGGTCCCCGAGCGCGTGCAg                     >  2:1464190/1‑150 (MQ=255)
                                                                                                                                  accaGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAg                     >  2:54232/1‑150 (MQ=255)
                                                                                                                                   ccagccagGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGATCCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAgg                    <  1:749456/150‑1 (MQ=255)
                                                                                                                                   ccagccagGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAgg                    <  1:942152/150‑1 (MQ=255)
                                                                                                                                   ccagccagGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGATGGAAATACTGGCTTCGGTCACCGATCGCGTTCAgg                    >  2:89477/1‑150 (MQ=255)
                                                                                                                                    cagccagGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAGAGACTGGCTTCGGTCACCGAGCGCGTTCCGgc                   >  1:1260277/1‑150 (MQ=255)
                                                                                                                                       ccagGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGACACCGATCGCGTTCAGGcgcg                <  2:502855/150‑1 (MQ=255)
                                                                                                                                        cagGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGt               >  1:618821/1‑150 (MQ=255)
                                                                                                                                         agGTGCTGGCGGCGCGGCAGCAGTGGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTc              <  2:1331291/150‑1 (MQ=255)
                                                                                                                                         aactGCTGGCGCGCCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAGAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTc              >  2:1177251/4‑150 (MQ=255)
                                                                                                                                         aactGCTGGCGCGCCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTc              >  2:1477840/4‑150 (MQ=255)
                                                                                                                                          actGCTGGCGCGCCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTcc             >  1:1067336/3‑150 (MQ=255)
                                                                                                                                           ctGCTGGCGCGCCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTCCg            >  2:65182/2‑150 (MQ=255)
                                                                                                                                           ctGCTGGCGCGCCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTCCg            >  1:905595/2‑150 (MQ=255)
                                                                                                                                             gCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTCCGGt          >  1:73483/1‑150 (MQ=255)
                                                                                                                                              cgggcggcGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAACAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGCCTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTCCGGTg         <  1:727702/148‑1 (MQ=255)
                                                                                                                                              cTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTCCGGTg         <  2:558496/150‑1 (MQ=255)
                                                                                                                                               tGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTCCGGTGc        <  2:1727643/150‑1 (MQ=255)
                                                                                                                                                 gcggcgCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTCCGGTGCCa      <  1:919893/150‑1 (MQ=255)
                                                                                                                                                     cgcgcCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTCCGGTGCCATCgg  >  2:475255/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTCCGGTGCCATCGG  >  NC_000913/493186‑493484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: