Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,831,764 C→G 10.2% A436A (GCC→GCG yicH → putative inner membrane‑anchored periplasmic AsmA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,831,7640CG10.2% 311.2 / 22.4 107A436A (GCC→GCGyicHputative inner membrane‑anchored periplasmic AsmA family protein
Reads supporting (aligned to +/- strand):  ref base C (49/46);  new base G (7/4);  total (56/51)
Fisher's exact test for biased strand distribution p-value = 5.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.92e-01

Only 100 of 122 total aligned reads displayed.

TAGCGCCAGCCGCAATCTGATCATTGATATCGACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCCACAACGTCAGAC  >  NC_000913/3831617‑3831913
                                                                                                                                                   |                                                                                                                                                     
tAGCGCCAGCCGCAATCTGATCAATCATAGCGCCCCTGACTTCCCTGCGCAGCTCACCGCGCTCGATGGCTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCcgccgccgc                                                                                                                                                     <  1:281237/150‑1 (MQ=255)
 aGCGCCAGCCGCAATCTGATCATTGATATCGACCCTGACTTCCCGTGGCAGCTCCCCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATgccgtcgccagt                                                                                                                                                    >  2:64668/1‑147 (MQ=255)
    gcCAGCCGCAATCTGATCATTGATATCGACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACa                                                                                                                                                 >  2:1440038/1‑150 (MQ=255)
    gcCAGCCGCAATCTGATCATTGATATCGACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACa                                                                                                                                                 >  2:752639/1‑150 (MQ=255)
    gcCAGCCGCAATCTGATCATTGATATCGACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGATACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACa                                                                                                                                                 <  2:1385609/150‑1 (MQ=255)
     ccAGCCGCAATCTGATCATTGATATCGACCCTGACTTCCCGGGGCAGCTCACCGCGCGCGATGGTTCCGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACAt                                                                                                                                                <  1:180534/150‑1 (MQ=255)
       aGCCGCAATCTGATCATTGATATCGACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACTCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGCATGCAGCCGCCGCGagaatc                                                                                                                                              >  2:277221/1‑145 (MQ=255)
        gCCGCAATCTGAGCATTGATATCGACCCTGACTTCCCGTGGCAGCTCACCGCGCGCGATGGCTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGCGCGTCTGGAGTGGCTCGGCGAATCTGAATGTCGCGGCGGCGGCATTCa                                                                                                                                             <  1:836217/150‑1 (MQ=255)
        cccGCAATCTGATCATTGATCTCGACCCTGACTTCCCGGGGCAGCTCACCGCGCTCGAGGGGTACGGTGCCAACCTGACGCTGGCTACCGATCATAAATGGGGCGGCTGGAGTGGCTCGGCGAATCTGAATGTCGCGGCGGCGGCATTCa                                                                                                                                             <  1:800424/149‑1 (MQ=255)
         ccGCAATCTGATCATTGATATCGACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGCCATTCaa                                                                                                                                            >  1:695721/1‑150 (MQ=255)
         ccGCAATCTGATCATTGATATCGACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCaa                                                                                                                                            >  2:141381/1‑150 (MQ=255)
           gCAATCTGATCATTGATATCGACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATc                                                                                                                                          <  1:387650/150‑1 (MQ=255)
           gCAATCTGATCATTGATATCGACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATc                                                                                                                                          >  2:1505806/1‑150 (MQ=255)
            cAATCTGATCATTGATATCGACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGAGAATCTGAATGCCGCCGCCGCGACCTTCAATCg                                                                                                                                         >  2:475055/1‑150 (MQ=255)
             aaTCTGATCATTGATCTCGACCCTGACTTCCCGTGGCAGCTCACCGCGCGCGATGGTTACGGTGCCAACCTGACTCTGGTTACCGAGCATAAATGGCGCGTCTGGAGTGTCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCgt                                                                                                                                        <  1:1255880/150‑1 (MQ=255)
             aaTCTGATCATTGATATCGACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCGCGGCGAATCTGAATGTCGCGGCGGCGGCATTCagattc                                                                                                                                        <  2:468289/150‑6 (MQ=255)
                cTGATCATTGATATCGACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGTTCATAAATGGGGCGTCTGGAGTGGCTCGGCGATTCTGAATGCCGCCGCCGCGACATTCAATCGTGtt                                                                                                                                     >  1:1341054/1‑150 (MQ=255)
                 tGATCATTGATATCGACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTTGCTCGGCGAATCTGAATGCCGCCGCCGCGCCATTCAATCGTGTTg                                                                                                                                    >  2:94833/1‑150 (MQ=255)
                 tGATCATTGATATCGACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTg                                                                                                                                    >  2:90572/1‑150 (MQ=255)
                 tGATCATTGATATCGACCCTGACATCCCGGTGCAGCTCCCCGCGCTCGATGGTTACGGTGCCAACCTGACGCTTGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTg                                                                                                                                    <  2:1210085/150‑1 (MQ=255)
                         gATATCGACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTtcgt                                                                                                                            >  2:755298/1‑150 (MQ=255)
                          atatCGACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATACATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACCTTCAATCGTGTTGATGTtcgtc                                                                                                                           >  1:263240/1‑150 (MQ=255)
                          atatCGACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTtcgtc                                                                                                                           <  1:768159/150‑1 (MQ=255)
                              cGACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGccc                                                                                                                       <  1:1702978/150‑1 (MQ=255)
                               gACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCg                                                                                                                      <  1:769517/150‑1 (MQ=255)
                                 cccTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGGTACCGATCATAAATGGGGCGGCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTc                                                                                                                    >  2:463713/1‑150 (MQ=255)
                                   cTGCCTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGGGCCAACCTGACGCGGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCGCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGc                                                                                                                  <  2:1703617/150‑1 (MQ=255)
                                   cTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGACAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGc                                                                                                                  >  2:76705/1‑150 (MQ=255)
                                    tGACTTCGCGTGGCAGCTCACCGCGCTCGTTGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAAGGGGGCGTCTGGAGTGGCGCGGCGAATCTGAATGCCGCCGCCGCGAGATTCAATCGTGTTGATGTTCGTCGCCCGTCGCt                                                                                                                 <  1:282042/150‑1 (MQ=255)
                                        ttCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGgcg                                                                                                             >  2:1682703/1‑150 (MQ=255)
                                         tCCCGTGGCAGCGCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCGCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGgcgc                                                                                                            <  1:1235134/150‑1 (MQ=255)
                                          cccGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCt                                                                                                           >  2:499659/1‑150 (MQ=255)
                                           ccGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTg                                                                                                          >  2:834402/1‑150 (MQ=255)
                                            cGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGa                                                                                                         >  2:943791/1‑150 (MQ=255)
                                              tGGCAGCTCACCGCGCTGGATGGTTTCGGTGCCAACCTGACGCTGGTTAGCGATCATCTGTGGGGCGTCTGGAGGGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTGGCTGTTCGTCGCCCGTCGCGGGCGCGGAcc                                                                                                       <  2:1163236/150‑1 (MQ=255)
                                              tGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGAcc                                                                                                       <  1:850632/150‑1 (MQ=255)
                                                  aGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCa                                                                                                   <  1:33355/150‑1 (MQ=255)
                                                    cTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAAc                                                                                                 <  1:1539585/150‑1 (MQ=255)
                                                    cTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAAc                                                                                                 <  1:1005558/150‑1 (MQ=255)
                                                      cACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAAcag                                                                                               >  2:1478217/1‑150 (MQ=255)
                                                         cgcgCTCGATGGGTAGGGTGCCAACCTGACGCTGGGTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCAGCGACATTCAATCGTGTTGATGGTCGTCGCCCGTCGCTGGCGGTGACCGCCAAcagcag                                                                                            <  2:413294/150‑1 (MQ=255)
                                                             cTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGt                                                                                                                     >  1:693305/1‑121 (MQ=255)
                                                             cTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGt                                                                                                                     <  2:693305/121‑1 (MQ=255)
                                                                gATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCCACAGCAGCACGGTg                                                                                     >  2:1552831/1‑150 (MQ=255)
                                                                  tGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGaa                                                                                   >  1:191073/1‑150 (MQ=255)
                                                                   ggTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAat                                                                                  <  2:532140/150‑1 (MQ=255)
                                                                      tACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACAGTGAATATc                                                                               >  1:1254536/1‑150 (MQ=255)
                                                                        cGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAg                                                                             <  2:575112/150‑1 (MQ=255)
                                                                          gTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGTGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAAACGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACTGCAGCACGGTGAATATCAGCg                                                                           >  2:1086235/1‑150 (MQ=255)
                                                                            gCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGaa                                                                         >  2:20509/1‑150 (MQ=255)
                                                                             ccAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAAc                                                                        <  2:481750/150‑1 (MQ=255)
                                                                              cAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACt                                                                       <  1:1440038/150‑1 (MQ=255)
                                                                               aaCCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTg                                                                      <  1:141381/150‑1 (MQ=255)
                                                                                  cTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGt                                                                   <  1:976086/150‑1 (MQ=255)
                                                                                  cTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGt                                                                   <  2:1713264/150‑1 (MQ=255)
                                                                                   tGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTg                                                                  >  1:411480/1‑150 (MQ=255)
                                                                                   tGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTg                                                                  <  1:706934/150‑1 (MQ=255)
                                                                                     aCGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCa                                                                >  1:48134/1‑150 (MQ=255)
                                                                                          ggTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAAc                                                                        >  1:493908/1‑137 (MQ=255)
                                                                                          ggTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTAc                                                           <  1:974037/150‑1 (MQ=255)
                                                                                          ggTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTAc                                                           >  1:1674668/1‑150 (MQ=255)
                                                                                            ttACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTg                                                         <  2:1062077/150‑1 (MQ=255)
                                                                                              aCCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGaa                                                       >  2:705644/1‑150 (MQ=255)
                                                                                                 gATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGATTATCAGCGAACTGAGTGCATTTACTGaaaaa                                                    >  1:212218/1‑150 (MQ=255)
                                                                                                 gATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGaaaaa                                                    >  1:1391309/1‑150 (MQ=255)
                                                                                                 gATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGaaaaa                                                    <  2:441598/150‑1 (MQ=255)
                                                                                                  aTCATAAATGGTGCGTCTGGAGTGGCTCGGCTAATCTGAATGCCGCCGCCTCGACATTCAATCGTGTTGATGTTCGTAGCCCGTCGCTGTCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAg                                                   <  1:894939/150‑1 (MQ=255)
                                                                                                      tAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCACGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATTTCAGCGAACTGAGTGCATTTACTGAAAACGGCAt                                               >  1:249006/1‑150 (MQ=255)
                                                                                                          tGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCTATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGAGCGCCAACAGCAGCACGGGGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTg                                           <  1:1148052/150‑1 (MQ=255)
                                                                                                            gggAGTCTGGAGTGGCTCTGCGAATCTGAATGCCGCCGCCGCGACTTTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCTCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGa                                         <  1:168225/150‑1 (MQ=255)
                                                                                                                        tGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAGCAGCAGCACGGTGAATATCAGCGAACTGAGTGCTTTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAg                             <  1:76705/150‑1 (MQ=255)
                                                                                                                        gggCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAg                             <  1:191101/149‑1 (MQ=255)
                                                                                                                         ggCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAgt                            >  1:1651206/1‑150 (MQ=255)
                                                                                                                         ggCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAgt                            >  2:240256/1‑150 (MQ=255)
                                                                                                                           cTCGTCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAgtgt                          <  1:1129042/150‑1 (MQ=255)
                                                                                                                           cTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAgtgt                          >  2:747225/1‑150 (MQ=255)
                                                                                                                                 acgattGAATGTCGCGGCGGCGGCATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGTAAGCCACCGCCAGTGTTTcaca                    >  2:405291/6‑150 (MQ=255)
                                                                                                                                    tCTGACTGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAAc                 <  2:1497768/150‑1 (MQ=255)
                                                                                                                                    tCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAAc                 >  2:1220013/1‑150 (MQ=255)
                                                                                                                                    tCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAAc                 >  1:380024/1‑150 (MQ=255)
                                                                                                                                     ttGAATGTCGCGGCGGCGGCATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACg                >  2:176362/2‑150 (MQ=255)
                                                                                                                                     ttGAATGTCGCGGCGGCGGCATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCCCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACAGTGTGCATTTAGTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGGTTCCCAAAcc                >  1:556129/2‑149 (MQ=255)
                                                                                                                                     cTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACg                >  1:1344800/1‑150 (MQ=255)
                                                                                                                                      tGAATGTCGCGGCGGCGGCATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGc               >  1:1355287/1‑150 (MQ=255)
                                                                                                                                      tGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGc               >  1:64668/1‑150 (MQ=255)
                                                                                                                                       gAATGTCGCGGCGGCGGCATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCCCCGCCAGTGTTTCACAAACGcc              >  1:1733347/1‑150 (MQ=255)
                                                                                                                                        aaTGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCca             >  2:66658/1‑150 (MQ=255)
                                                                                                                                         aTGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCcac            <  2:538682/150‑1 (MQ=255)
                                                                                                                                         aTGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCcac            <  1:755298/150‑1 (MQ=255)
                                                                                                                                         aTGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCcac            >  2:1113999/1‑150 (MQ=255)
                                                                                                                                              gccgccgcGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCCACAACgg       >  2:1241894/1‑149 (MQ=255)
                                                                                                                                              gccgccgcGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCCACAACGt       >  2:1215587/1‑150 (MQ=255)
                                                                                                                                              gccgccgcGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCCACAACGt       <  1:1201290/150‑1 (MQ=255)
                                                                                                                                                cgccgcGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCCACAACGTCa     <  2:191073/150‑1 (MQ=255)
                                                                                                                                                cgccgcGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCAACCGCCAGTGTTTCACAAACGCCACAACGTCa     >  2:94860/1‑150 (MQ=255)
                                                                                                                                                 gccgcGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCCACAACGTCAg    <  1:834402/150‑1 (MQ=255)
                                                                                                                                                 gccgcGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCCACAACGTCAg    <  1:100047/150‑1 (MQ=255)
                                                                                                                                                 gccgcGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCCACAACGTCAg    <  1:569736/150‑1 (MQ=255)
                                                                                                                                                 gccgcGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCCACAACGTCAg    <  2:506922/150‑1 (MQ=255)
                                                                                                                                                   cgcgACATTCAATCGTGTTGATGTTCGTCGCCCGTCTCTGGCGCTGACCGCCAACAGCATCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCCACAACGTCAGac  <  1:475055/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TAGCGCCAGCCGCAATCTGATCATTGATATCGACCCTGACTTCCCGTGGCAGCTCACCGCGCTCGATGGTTACGGTGCCAACCTGACGCTGGTTACCGATCATAAATGGGGCGTCTGGAGTGGCTCGGCGAATCTGAATGCCGCCGCCGCGACATTCAATCGTGTTGATGTTCGTCGCCCGTCGCTGGCGCTGACCGCCAACAGCAGCACGGTGAATATCAGCGAACTGAGTGCATTTACTGAAAAAGGCATTCTGGAAGCCACCGCCAGTGTTTCACAAACGCCACAACGTCAGAC  >  NC_000913/3831617‑3831913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: