Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,843,569 A→G 5.0% I74T (ATC→ACC)  adeQ ← adenine permease, high affinity; adenine:H+ symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,843,5690AG 5.0% 291.9 / 4.7 100I74T (ATC→ACC) adeQadenine permease, high affinity; adenine:H+ symporter
Reads supporting (aligned to +/- strand):  ref base A (38/55);  new base G (3/2);  total (43/57)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01

Only 100 of 101 total aligned reads displayed.

CAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCATG  >  NC_000913/3843420‑3843717
                                                                                                                                                     |                                                                                                                                                    
cAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGa                                                                                                                                                      <  2:806935/150‑1 (MQ=255)
 aaCTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGAt                                                                                                                                                     <  1:1540331/150‑1 (MQ=255)
    tGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATAcc                                                                                                                                                  <  1:1636280/150‑1 (MQ=255)
    tGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTGCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATAcc                                                                                                                                                  <  2:88743/150‑1 (MQ=255)
       gcCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGc                                                                                                                                               >  1:1028527/1‑150 (MQ=255)
         cccAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCCGCGCCCCGGGGAAGTTAGCAAATATCCCCCTCGCAATTCTGCCGATACCGGCaa                                                                                                                                             >  1:817604/1‑150 (MQ=255)
          ccAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGGTATCGGCagt                                                                                                                                            <  2:809069/150‑3 (MQ=255)
          ccAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCaag                                                                                                                                            >  1:991615/1‑149 (MQ=255)
          ccAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAAt                                                                                                                                            >  1:998152/1‑150 (MQ=255)
          ccAGAATATTGCGCACATCCCGGTCTGCCAGGAGATGCGCAGAGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCCGAGCCAGCGACACGGGTAAGTTAGCAAAATTCCGCAACGCAATACTGCCGATACCGGCatg                                                                                                                                            >  2:71414/1‑148 (MQ=255)
          ccAGAATATTGCGACCATCCCGGTCTGCCAGGTGATGCCCATCGCCCCGACGACCACGAAGGCTAAGAATGCGTTCAGCGCCATTGCCGGAGCCAGCGCCACTGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAAt                                                                                                                                            <  1:153179/150‑1 (MQ=255)
            aGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCa                                                                                                                                          <  1:1312104/150‑1 (MQ=255)
              aataatGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCAATGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCAATCGCTATACTGCCGATACCGGCAATCaaa                                                                                                                                        <  1:250463/150‑1 (MQ=255)
              aaTATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGGTATCGGCagtattg                                                                                                                                        <  2:1299427/150‑7 (MQ=255)
              aaTATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCaaa                                                                                                                                        >  2:70782/1‑150 (MQ=255)
               atatTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAAc                                                                                                                                       <  2:132043/150‑1 (MQ=255)
                tatTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACa                                                                                                                                      >  2:1384387/1‑150 (MQ=255)
                tatTGCGCCCATCCCGGGCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACa                                                                                                                                      <  2:532874/150‑1 (MQ=255)
                  ttGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAgg                                                                                                                                    <  1:376370/150‑1 (MQ=255)
                    gcgcCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAggtg                                                                                                                                  <  1:1396293/150‑1 (MQ=255)
                    gcgcCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAggtg                                                                                                                                  <  1:50517/150‑1 (MQ=255)
                    gcgcCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAggtg                                                                                                                                  >  1:521952/1‑150 (MQ=255)
                      gcCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAggtggt                                                                                                                                <  1:482583/150‑1 (MQ=255)
                         cATCCCGTTCTGACAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCAGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTaac                                                                                                                             <  1:977833/150‑1 (MQ=255)
                         cATCCCGGTCAGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTaac                                                                                                                             <  1:187352/150‑1 (MQ=255)
                          aTCCCGGGCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTaaca                                                                                                                            <  1:1083720/150‑1 (MQ=255)
                             ccGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGATGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCTGCGCCACGTGTAAGTAAGCAAATACCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTaacaaac                                                                                                                         <  1:433200/150‑1 (MQ=255)
                             ccGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTCGCAAATATCCCCATCGCAATACTGCCGCTACCGGCAATCAAACAGGTGGTaacaacc                                                                                                                         >  2:289789/1‑148 (MQ=255)
                               ggTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAacac                                                                                                                       <  1:1534105/150‑1 (MQ=255)
                               ggTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAacac                                                                                                                       <  1:1447234/150‑1 (MQ=255)
                                 tCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAAcacca                                                                                                                     <  2:1377184/150‑1 (MQ=255)
                                 tCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAAcacca                                                                                                                     <  2:1319731/150‑1 (MQ=255)
                                  cTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAAcaccac                                                                                                                    <  2:604828/150‑1 (MQ=255)
                                   tGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACt                                                                                                                   <  1:1269829/150‑1 (MQ=255)
                                    gCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACACACACCACtt                                                                                                                  >  1:1563200/1‑150 (MQ=255)
                                    gCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACtt                                                                                                                  <  1:548164/150‑1 (MQ=255)
                                      cAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGCGCCAGCGCCCCGGGTAAGTTAGCAAATATCCCCCTCGCAATACTGCCGCTACCGGCAATCCAACAGGTGGTAACAACCACCACTTTc                                                                                                                >  1:131025/1‑150 (MQ=255)
                                           gaTGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCCGCGCCCCGGGTAAGTTAGCAAATATCCCCCTCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTc                                                                                                           >  1:1048489/1‑150 (MQ=255)
                                           gaTGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTc                                                                                                           <  2:701153/150‑1 (MQ=255)
                                           gaTGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTc                                                                                                           >  2:669913/1‑150 (MQ=255)
                                            aTGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTcc                                                                                                          <  2:1365004/150‑1 (MQ=255)
                                             tGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACTGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCa                                                                                                         <  1:413752/150‑1 (MQ=255)
                                             tGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGATAGCAAATAACCCCATCGCAATACTGCCGATAACGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGCTCCa                                                                                                         >  2:1476550/1‑150 (MQ=255)
                                               cccATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCAtt                                                                                                       <  1:1031302/150‑1 (MQ=255)
                                                 cATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTtg                                                                                                     <  1:1471359/150‑1 (MQ=255)
                                                  aTCGCCCCCACGCCCACGAACGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAACCAACACTTTCGGGTCCATTtgt                                                                                                    >  2:1400158/1‑150 (MQ=255)
                                                   tCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTtgtg                                                                                                   <  1:597153/150‑1 (MQ=255)
                                                     gCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCAGGGGTAAGTTAGCAAATATCCCCATCGCAATACTGGCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGcc                                                                                                 >  2:1175420/1‑150 (MQ=255)
                                                      cccccACGACCACGAAGGCAAAGAAGGCGGTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCcg                                                                                                <  2:1553027/150‑1 (MQ=255)
                                                             gaccacgaAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAg                                                                                         <  1:665055/150‑1 (MQ=255)
                                                             gaccacgaAGGCAAAGAAGGCGGTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAg                                                                                         <  1:1503640/150‑1 (MQ=255)
                                                              accacgaAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGATCCATTTGTGCCGCGCCGAgg                                                                                        >  2:1116620/1‑150 (MQ=255)
                                                              accacgaAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCACCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAgg                                                                                        >  2:1521013/1‑150 (MQ=255)
                                                                 acgaAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGAtt                                                                                     >  2:763203/1‑150 (MQ=255)
                                                                       gCAAAGACGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGAGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCggg                                                                               <  2:519988/150‑1 (MQ=255)
                                                                       gCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCggg                                                                               >  1:610027/1‑150 (MQ=255)
                                                                        cAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGt                                                                              >  1:1646556/1‑150 (MQ=255)
                                                                        cAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGt                                                                              <  2:537063/150‑1 (MQ=255)
                                                                          aagaagGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTc                                                                            <  2:204291/150‑1 (MQ=255)
                                                                            gaagGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGTTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGATcac                                                                          >  1:1235813/1‑150 (MQ=255)
                                                                             aagGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTcaca                                                                         >  1:1704124/1‑150 (MQ=255)
                                                                             aacgCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGAAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTcaca                                                                         <  1:251996/147‑1 (MQ=255)
                                                                                 cGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACaaaaa                                                                     >  2:786563/1‑150 (MQ=255)
                                                                                  gTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAAc                                                                    <  2:1466022/150‑1 (MQ=255)
                                                                                   ttCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACg                                                                   <  1:1243560/150‑1 (MQ=255)
                                                                                      aGCCCCATTGCTGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGAGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATg                                                                <  2:1319284/150‑1 (MQ=255)
                                                                                       gCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGt                                                               <  1:346626/150‑1 (MQ=255)
                                                                                       gCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTGCACAAAAACGATGt                                                               <  2:1535024/150‑1 (MQ=255)
                                                                                        ccccATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGTTGGTAACAAACACCACTTTCGGGTCCATTTCTGCCGCGCCGAGGATTTGAGGGTTCACAAAAACGATGTa                                                              >  1:780019/1‑150 (MQ=255)
                                                                                            aTTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACAcc                                                          <  2:1636060/150‑1 (MQ=255)
                                                                                               gCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATg                                                       >  2:1411437/1‑150 (MQ=255)
                                                                                                ccGGAGCCAGCGCCAAGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATgg                                                      <  2:591068/150‑1 (MQ=255)
                                                                                                 cGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCTTGACCATTTGTGGCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGt                                                     <  1:1384387/150‑1 (MQ=255)
                                                                                                 cGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGt                                                     >  1:1262229/1‑150 (MQ=255)
                                                                                                  ggAGCCATCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGtt                                                    <  2:131025/150‑1 (MQ=255)
                                                                                                       cAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGAAACCGGCAATCAAACAGGTGGTAACAAACACCCCTTTCGGGTCCAATTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTaaaaa                                               >  1:264581/1‑150 (MQ=255)
                                                                                                         gcgcCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAg                                             <  1:817222/150‑1 (MQ=255)
                                                                                                          cgcCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGt                                            >  1:573704/1‑150 (MQ=255)
                                                                                                          cgcCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACATTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGt                                            <  1:46882/150‑1 (MQ=255)
                                                                                                           gcCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTg                                           <  1:588758/150‑1 (MQ=255)
                                                                                                           gcCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGGCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTg                                           <  2:1624710/150‑1 (MQ=255)
                                                                                                            ccACGTGTAAGTTAGCAAATATCCCCATCGCATTACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCAGAGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTgg                                          <  1:1180861/150‑1 (MQ=255)
                                                                                                            ccACGTGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTTGTAACAAACACCTCTTTCGGGTCCATTTGTTCCGCGCCGAGGATTTGCTGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTgg                                          <  1:472212/150‑1 (MQ=255)
                                                                                                            ccACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCCCAAAAACGATGTACACCATGGTTAAAAAAGTgg                                          >  1:1508078/1‑150 (MQ=255)
                                                                                                            ccACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTgg                                          <  2:610027/150‑1 (MQ=255)
                                                                                                                   tAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATccc                                   >  1:897769/1‑150 (MQ=255)
                                                                                                                   tAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATccc                                   <  1:641677/150‑1 (MQ=255)
                                                                                                                     aGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCg                                 >  2:620550/1‑150 (MQ=255)
                                                                                                                     aGTTAGCAAATATCCCCATCGCAATACTGCCGAGACCGGCACTCAAACAGGTGGTAACATACACCACTTTCGTGTCCATTTGTGCCGCGAAGAGGATTTGCGGGTTGACAAAAACGATGTACACAATTGTTAAAAAAGTTGTCGTCCCCg                                 >  2:289797/1‑150 (MQ=255)
                                                                                                                       ttAGCAAATATCCCCATAGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGca                               >  2:1128486/1‑150 (MQ=255)
                                                                                                                        tAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATACCCGcaa                              >  2:806164/1‑150 (MQ=255)
                                                                                                                         aGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGcaat                             >  1:1238019/1‑150 (MQ=255)
                                                                                                                           cAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGcaatca                           >  1:989493/1‑150 (MQ=255)
                                                                                                                                         gtttgattGCCGGTATCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGACg             >  1:736198/8‑150 (MQ=255)
                                                                                                                                            tgattGCCGGTATCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGACggtg          >  1:1052440/5‑150 (MQ=255)
                                                                                                                                            aTACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATACCCGCAATCAATTCTGTGCGGACggtg          >  1:1345068/1‑150 (MQ=255)
                                                                                                                                              aCTGCCGATACCGGCAATCAAACAGGTGGTAACAAACCCCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGGTCACAAAAACGATGTACACCATGGTTAATAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGACggtggt        >  2:102482/1‑150 (MQ=255)
                                                                                                                                              aCTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGAccaccgt        <  1:620550/150‑7 (MQ=255)
                                                                                                                                                  ccGGTATCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCa    >  1:424662/1‑150 (MQ=255)
                                                                                                                                                    gATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCATg  <  1:70782/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CAACTGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCATG  >  NC_000913/3843420‑3843717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: