Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,843,572 C→T 5.2% G73D (GGT→GAT)  adeQ ← adenine permease, high affinity; adenine:H+ symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,843,5720CT 5.2% 308.2 / 4.0 97G73D (GGT→GAT) adeQadenine permease, high affinity; adenine:H+ symporter
Reads supporting (aligned to +/- strand):  ref base C (39/52);  new base T (3/2);  total (43/54)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01

Only 100 of 101 total aligned reads displayed.

TGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGA  >  NC_000913/3843424‑3843721
                                                                                                                                                    |                                                                                                                                                     
tGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATAcc                                                                                                                                                      <  1:1636280/150‑1 (MQ=255)
tGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTGCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATAcc                                                                                                                                                      <  2:88743/150‑1 (MQ=255)
   gcCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGc                                                                                                                                                   >  1:1028527/1‑150 (MQ=255)
     cccAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCCGCGCCCCGGGGAAGTTAGCAAATATCCCCCTCGCAATTCTGCCGATACCGGCaa                                                                                                                                                 >  1:817604/1‑150 (MQ=255)
      ccAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGGTATCGGCagt                                                                                                                                                <  2:809069/150‑3 (MQ=255)
      ccAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCaag                                                                                                                                                >  1:991615/1‑149 (MQ=255)
      ccAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAAt                                                                                                                                                >  1:998152/1‑150 (MQ=255)
      ccAGAATATTGCGCACATCCCGGTCTGCCAGGAGATGCGCAGAGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCCGAGCCAGCGACACGGGTAAGTTAGCAAAATTCCGCAACGCAATACTGCCGATACCGGCatg                                                                                                                                                >  2:71414/1‑148 (MQ=255)
      ccAGAATATTGCGACCATCCCGGTCTGCCAGGTGATGCCCATCGCCCCGACGACCACGAAGGCTAAGAATGCGTTCAGCGCCATTGCCGGAGCCAGCGCCACTGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAAt                                                                                                                                                <  1:153179/150‑1 (MQ=255)
        aGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCa                                                                                                                                              <  1:1312104/150‑1 (MQ=255)
          aataatGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCAATGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCAATCGCTATACTGCCGATACCGGCAATCaaa                                                                                                                                            <  1:250463/150‑1 (MQ=255)
          aaTATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGGTATCGGCagtattg                                                                                                                                            <  2:1299427/150‑7 (MQ=255)
          aaTATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCaaa                                                                                                                                            >  2:70782/1‑150 (MQ=255)
           atatTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAAc                                                                                                                                           <  2:132043/150‑1 (MQ=255)
            tatTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACa                                                                                                                                          >  2:1384387/1‑150 (MQ=255)
            tatTGCGCCCATCCCGGGCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACa                                                                                                                                          <  2:532874/150‑1 (MQ=255)
              ttGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAgg                                                                                                                                        <  1:376370/150‑1 (MQ=255)
                gcgcCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAggtg                                                                                                                                      <  1:1396293/150‑1 (MQ=255)
                gcgcCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAggtg                                                                                                                                      <  1:50517/150‑1 (MQ=255)
                gcgcCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAggtg                                                                                                                                      >  1:521952/1‑150 (MQ=255)
                  gcCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAggtggt                                                                                                                                    <  1:482583/150‑1 (MQ=255)
                     cATCCCGTTCTGACAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCAGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTaac                                                                                                                                 <  1:977833/150‑1 (MQ=255)
                     cATCCCGGTCAGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTaac                                                                                                                                 <  1:187352/150‑1 (MQ=255)
                      aTCCCGGGCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTaaca                                                                                                                                <  1:1083720/150‑1 (MQ=255)
                         ccGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGATGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCTGCGCCACGTGTAAGTAAGCAAATACCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTaacaaac                                                                                                                             <  1:433200/150‑1 (MQ=255)
                         ccGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTCGCAAATATCCCCATCGCAATACTGCCGCTACCGGCAATCAAACAGGTGGTaacaacc                                                                                                                             >  2:289789/1‑148 (MQ=255)
                           ggTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAacac                                                                                                                           <  1:1534105/150‑1 (MQ=255)
                           ggTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAacac                                                                                                                           <  1:1447234/150‑1 (MQ=255)
                             tCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAAcacca                                                                                                                         <  2:1377184/150‑1 (MQ=255)
                             tCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAAcacca                                                                                                                         <  2:1319731/150‑1 (MQ=255)
                              cTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAAcaccac                                                                                                                        <  2:604828/150‑1 (MQ=255)
                               tGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACt                                                                                                                       <  1:1269829/150‑1 (MQ=255)
                                gCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACACACACCACtt                                                                                                                      >  1:1563200/1‑150 (MQ=255)
                                gCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACtt                                                                                                                      <  1:548164/150‑1 (MQ=255)
                                  cAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGCGCCAGCGCCCCGGGTAAGTTAGCAAATATCCCCCTCGCAATACTGCCGCTACCGGCAATCCAACAGGTGGTAACAACCACCACTTTc                                                                                                                    >  1:131025/1‑150 (MQ=255)
                                       gaTGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCCGCGCCCCGGGTAAGTTAGCAAATATCCCCCTCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTc                                                                                                               >  1:1048489/1‑150 (MQ=255)
                                       gaTGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTc                                                                                                               >  2:669913/1‑150 (MQ=255)
                                       gaTGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTc                                                                                                               <  2:701153/150‑1 (MQ=255)
                                        aTGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTcc                                                                                                              <  2:1365004/150‑1 (MQ=255)
                                         tGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACTGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCa                                                                                                             <  1:413752/150‑1 (MQ=255)
                                         tGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGATAGCAAATAACCCCATCGCAATACTGCCGATAACGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGCTCCa                                                                                                             >  2:1476550/1‑150 (MQ=255)
                                           cccATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCAtt                                                                                                           <  1:1031302/150‑1 (MQ=255)
                                             cATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTtg                                                                                                         <  1:1471359/150‑1 (MQ=255)
                                              aTCGCCCCCACGCCCACGAACGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAACCAACACTTTCGGGTCCATTtgt                                                                                                        >  2:1400158/1‑150 (MQ=255)
                                               tCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTtgtg                                                                                                       <  1:597153/150‑1 (MQ=255)
                                                 gCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCAGGGGTAAGTTAGCAAATATCCCCATCGCAATACTGGCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGcc                                                                                                     >  2:1175420/1‑150 (MQ=255)
                                                  cccccACGACCACGAAGGCAAAGAAGGCGGTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCcg                                                                                                    <  2:1553027/150‑1 (MQ=255)
                                                         gaccacgaAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAg                                                                                             <  1:665055/150‑1 (MQ=255)
                                                         gaccacgaAGGCAAAGAAGGCGGTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAg                                                                                             <  1:1503640/150‑1 (MQ=255)
                                                          accacgaAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGATCCATTTGTGCCGCGCCGAgg                                                                                            >  2:1116620/1‑150 (MQ=255)
                                                          accacgaAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCACCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAgg                                                                                            >  2:1521013/1‑150 (MQ=255)
                                                             acgaAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGAtt                                                                                         >  2:763203/1‑150 (MQ=255)
                                                                   gCAAAGACGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGAGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCggg                                                                                   <  2:519988/150‑1 (MQ=255)
                                                                   gCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCggg                                                                                   >  1:610027/1‑150 (MQ=255)
                                                                    cAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGt                                                                                  >  1:1646556/1‑150 (MQ=255)
                                                                    cAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGt                                                                                  <  2:537063/150‑1 (MQ=255)
                                                                      aagaagGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTc                                                                                <  2:204291/150‑1 (MQ=255)
                                                                        gaagGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGTTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGATcac                                                                              >  1:1235813/1‑150 (MQ=255)
                                                                         aagGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTcaca                                                                             >  1:1704124/1‑150 (MQ=255)
                                                                         aacgCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGAAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTcaca                                                                             <  1:251996/147‑1 (MQ=255)
                                                                             cGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACaaaaa                                                                         >  2:786563/1‑150 (MQ=255)
                                                                              gTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAAc                                                                        <  2:1466022/150‑1 (MQ=255)
                                                                               ttCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACg                                                                       <  1:1243560/150‑1 (MQ=255)
                                                                                  aGCCCCATTGCTGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGAGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATg                                                                    <  2:1319284/150‑1 (MQ=255)
                                                                                   gCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGt                                                                   <  1:346626/150‑1 (MQ=255)
                                                                                   gCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTGCACAAAAACGATGt                                                                   <  2:1535024/150‑1 (MQ=255)
                                                                                    ccccATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGTTGGTAACAAACACCACTTTCGGGTCCATTTCTGCCGCGCCGAGGATTTGAGGGTTCACAAAAACGATGTa                                                                  >  1:780019/1‑150 (MQ=255)
                                                                                        aTTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACAcc                                                              <  2:1636060/150‑1 (MQ=255)
                                                                                           gCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATg                                                           >  2:1411437/1‑150 (MQ=255)
                                                                                            ccGGAGCCAGCGCCAAGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATgg                                                          <  2:591068/150‑1 (MQ=255)
                                                                                             cGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCTTGACCATTTGTGGCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGt                                                         <  1:1384387/150‑1 (MQ=255)
                                                                                             cGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGt                                                         >  1:1262229/1‑150 (MQ=255)
                                                                                              ggAGCCATCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGtt                                                        <  2:131025/150‑1 (MQ=255)
                                                                                                   cAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGAAACCGGCAATCAAACAGGTGGTAACAAACACCCCTTTCGGGTCCAATTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTaaaaa                                                   >  1:264581/1‑150 (MQ=255)
                                                                                                     gcgcCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAg                                                 <  1:817222/150‑1 (MQ=255)
                                                                                                      cgcCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGt                                                >  1:573704/1‑150 (MQ=255)
                                                                                                      cgcCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACATTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGt                                                <  1:46882/150‑1 (MQ=255)
                                                                                                       gcCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTg                                               <  1:588758/150‑1 (MQ=255)
                                                                                                       gcCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGGCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTg                                               <  2:1624710/150‑1 (MQ=255)
                                                                                                        ccACGTGTAAGTTAGCAAATATCCCCATCGCATTACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCAGAGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTgg                                              <  1:1180861/150‑1 (MQ=255)
                                                                                                        ccACGTGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTTGTAACAAACACCTCTTTCGGGTCCATTTGTTCCGCGCCGAGGATTTGCTGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTgg                                              <  1:472212/150‑1 (MQ=255)
                                                                                                        ccACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCCCAAAAACGATGTACACCATGGTTAAAAAAGTgg                                              >  1:1508078/1‑150 (MQ=255)
                                                                                                        ccACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTgg                                              <  2:610027/150‑1 (MQ=255)
                                                                                                               tAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATccc                                       >  1:897769/1‑150 (MQ=255)
                                                                                                               tAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATccc                                       <  1:641677/150‑1 (MQ=255)
                                                                                                                 aGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCg                                     >  2:620550/1‑150 (MQ=255)
                                                                                                                 aGTTAGCAAATATCCCCATCGCAATACTGCCGAGACCGGCACTCAAACAGGTGGTAACATACACCACTTTCGTGTCCATTTGTGCCGCGAAGAGGATTTGCGGGTTGACAAAAACGATGTACACAATTGTTAAAAAAGTTGTCGTCCCCg                                     >  2:289797/1‑150 (MQ=255)
                                                                                                                   ttAGCAAATATCCCCATAGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGca                                   >  2:1128486/1‑150 (MQ=255)
                                                                                                                    tAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATACCCGcaa                                  >  2:806164/1‑150 (MQ=255)
                                                                                                                     aGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGcaat                                 >  1:1238019/1‑150 (MQ=255)
                                                                                                                       cAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGcaatca                               >  1:989493/1‑150 (MQ=255)
                                                                                                                                     gtttgattGCCGGTATCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGACg                 >  1:736198/8‑150 (MQ=255)
                                                                                                                                        tgattGCCGGTATCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGACggtg              >  1:1052440/5‑150 (MQ=255)
                                                                                                                                        aTACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATACCCGCAATCAATTCTGTGCGGACggtg              >  1:1345068/1‑150 (MQ=255)
                                                                                                                                          aCTGCCGATACCGGCAATCAAACAGGTGGTAACAAACCCCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGGTCACAAAAACGATGTACACCATGGTTAATAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGACggtggt            >  2:102482/1‑150 (MQ=255)
                                                                                                                                          aCTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGAccaccgt            <  1:620550/150‑7 (MQ=255)
                                                                                                                                              ccGGTATCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCa        >  1:424662/1‑150 (MQ=255)
                                                                                                                                                gATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCATg      <  1:70782/150‑1 (MQ=255)
                                                                                                                                                    ccGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGa  <  1:1707531/150‑1 (MQ=255)
                                                                                                                                                    ccGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGa  >  2:1704612/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGCGCCCCAGAATATTGCGCCCATCCCGGTCTGCCAGGAGATGCCCATCGCCCCCACGACCACGAAGGCAAAGAAGGCGTTCAGCCCCATTGCCGGAGCCAGCGCCACGGGTAAGTTAGCAAATATCCCCATCGCAATACTGCCGATACCGGCAATCAAACAGGTGGTAACAAACACCACTTTCGGGTCCATTTGTGCCGCGCCGAGGATTTGCGGGTTCACAAAAACGATGTACACCATGGTTAAAAAAGTGGTCATCCCCGCAATCAATTCTGTGCGGACGGTGGTCCCATGCTGA  >  NC_000913/3843424‑3843721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: