Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 493,336 G→C 9.2% A415A (GCG→GCC) ‡ dnaX → DNA polymerase III/DNA elongation factor III, tau and gamma subunits

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913493,3360GC 9.2% 272.8 / 14.9 87A415A (GCG→GCC) ‡dnaXDNA polymerase III/DNA elongation factor III, tau and gamma subunits
Reads supporting (aligned to +/- strand):  ref base G (42/37);  new base C (5/3);  total (47/40)
Fisher's exact test for biased strand distribution p-value = 7.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

CTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGC  >  NC_000913/493188‑493485
                                                                                                                                                    |                                                                                                                                                     
cTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGCCTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGgcgc                                                                                                                                                      >  1:777355/1‑150 (MQ=255)
 tGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGgcgcg                                                                                                                                                     >  2:1174887/1‑150 (MQ=255)
    gCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGCCGCCAACCCAGGTGCCGCCGCACCCGCAATCAGCGCCGCAGCCGGCACCGCCTGTACCGCTCCCGGAAACCCCCCGCCAGGTGCTGGCGGCGCGccc                                                                                                                                                  >  2:52265/1‑149 (MQ=255)
    gCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGccc                                                                                                                                                  >  1:483518/1‑149 (MQ=255)
     ccAGAAGTGCCACGACAGTCCTTTGCTCCCGTCGCGCCAACGGCAGTAATGCCGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCCCCAGCCAGGTGCTGGCGGCGCGCcag                                                                                                                                                 >  2:501811/1‑150 (MQ=255)
     ccAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCcag                                                                                                                                                 >  2:1186088/1‑150 (MQ=255)
      cAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGCTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCAAGGTGCTGGCGCGCCGCcagc                                                                                                                                                <  2:1209759/150‑1 (MQ=255)
       aGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCcagca                                                                                                                                               >  2:1730737/1‑150 (MQ=255)
       aGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGCGCCGCcagca                                                                                                                                               <  2:504296/150‑1 (MQ=255)
         acgTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGCGCCGCcagcacc                                                                                                                                             <  2:1176573/148‑3 (MQ=255)
            tGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGc                                                                                                                                          <  2:1362569/150‑1 (MQ=255)
            tGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGc                                                                                                                                          <  2:1516177/150‑1 (MQ=255)
             gCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCACCCGCAATCAGCGCCGCAGCAGGCACCGCCTGTACCGCTCCCGGAACCCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCa                                                                                                                                         >  2:949435/1‑150 (MQ=255)
             gCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCa                                                                                                                                         >  2:1386678/1‑150 (MQ=255)
              ccccGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAg                                                                                                                                        >  2:1186929/4‑150 (MQ=255)
              ccACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCACCCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAg                                                                                                                                        >  2:749456/1‑150 (MQ=255)
               cACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGCCGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCCGCCAGGTGCTGGCGGCGCGCCCGCAGTTGCAgc                                                                                                                                       >  2:1049589/1‑150 (MQ=255)
                   aCAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGGGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCCGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGt                                                                                                                                   <  1:790245/150‑1 (MQ=255)
                        ccTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGACACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAgg                                                                                                                              >  1:502855/1‑150 (MQ=255)
                             gCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCa                                                                                                                         <  1:600720/150‑1 (MQ=255)
                                cccGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAAcc                                                                                                                      <  2:1431094/150‑1 (MQ=255)
                                cccGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGAGTGCAGGGAGCAAcc                                                                                                                      >  2:1274904/1‑150 (MQ=255)
                                   gTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGCAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCcaa                                                                                                                   >  2:870249/1‑150 (MQ=255)
                                      gcgcCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCa                                                                                                                <  1:1386678/150‑1 (MQ=255)
                                        gcCAACGGCAGTAATGACGCCAACCCCGGTGCCGCCGCAACCGCAATCCGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCCGCGCGGGCGGGGAGCACCCAAAGCaaa                                                                                                              >  2:727702/1‑150 (MQ=255)
                                        gcCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCTGGGAGCAACCAAAGCaaa                                                                                                              <  2:1709596/150‑1 (MQ=255)
                                         ccAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCaaaa                                                                                                             >  2:1495628/1‑150 (MQ=255)
                                            aCGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAag                                                                                                          <  2:1518177/150‑1 (MQ=255)
                                             cGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAaga                                                                                                         >  1:1727643/1‑150 (MQ=255)
                                                cAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTg                                                                                                      <  2:599561/150‑1 (MQ=255)
                                                  gTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGCGTGaa                                                                                                    >  1:1267916/1‑150 (MQ=255)
                                                   tAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAAc                                                                                                   <  1:179809/150‑1 (MQ=255)
                                                     aTGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCg                                                                                                 <  1:352107/150‑1 (MQ=255)
                                                      tGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCgg                                                                                                >  2:1340377/1‑150 (MQ=255)
                                                      tGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCgg                                                                                                <  2:892022/150‑1 (MQ=255)
                                                            cAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCg                                                                                          >  2:1653252/1‑150 (MQ=255)
                                                             aaCCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCATCAGCCAGGTGCTGGCGGCGCGCGAGCAGTTGCAGCGCGTGCAGTGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGc                                                                                         <  1:295129/150‑1 (MQ=255)
                                                              aCCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCt                                                                                        <  1:1160240/150‑1 (MQ=255)
                                                                   ggTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCcg                                                                                   >  1:1120282/1‑150 (MQ=255)
                                                                     ggccgccGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCcgcg                                                                                 <  2:1408302/149‑1 (MQ=255)
                                                                         gccgcAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCcgcgcgcg                                                                             >  1:1336029/1‑150 (MQ=255)
                                                                               accgcaaTCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCTCCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCTGCAGCCGCTACCCGCGCGCGGCCGGt                                                                       <  1:1454186/150‑1 (MQ=255)
                                                                                ccgcaaTCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCCCCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTg                                                                      <  1:187894/150‑1 (MQ=255)
                                                                                ccgcaaTCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCCGCCGCTACCCGCGCGCGGCCGGTg                                                                      >  2:1637602/1‑150 (MQ=255)
                                                                                ccgcaaTCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTg                                                                      <  2:742917/150‑1 (MQ=255)
                                                                                ccgcaaTCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGACAGCCGCTACCCGCGCGCGGCCGGTg                                                                      >  1:576142/1‑150 (MQ=255)
                                                                                  gcaaTCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGaa                                                                    >  1:768850/1‑150 (MQ=255)
                                                                                   caaTCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAAt                                                                   >  1:127299/1‑150 (MQ=255)
                                                                                         gcgcCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCATGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAAcgcg                                                             >  2:472737/1‑149 (MQ=255)
                                                                                           gccgcAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCAGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGATCCGGCAGCCGCTACCCGCGCGCGGCCGGTAATTAAcgctgc                                                           <  2:759426/150‑1 (MQ=255)
                                                                                              gcagcaGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAAcgctgcgct                                                        >  2:754913/1‑150 (MQ=255)
                                                                                               cagcagGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGTGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAAcgctgcgctg                                                       <  2:300874/150‑1 (MQ=255)
                                                                                               cagcagGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAAcgctgcgctg                                                       <  2:1728343/150‑1 (MQ=255)
                                                                                                agcagGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTgg                                                      <  2:1257624/150‑1 (MQ=255)
                                                                                                    ggCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGTATAACGCTGCGCTGGAAAg                                                  >  2:1230733/1‑150 (MQ=255)
                                                                                                       aCCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACt                                               <  1:552607/150‑1 (MQ=255)
                                                                                                         cGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTgg                                             <  2:1202032/150‑1 (MQ=255)
                                                                                                          gACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGc                                            >  1:448292/1‑150 (MQ=255)
                                                                                                          gACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGc                                            <  2:257192/150‑1 (MQ=255)
                                                                                                           aCTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCt                                           <  1:1730737/150‑1 (MQ=255)
                                                                                                              gTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCg                                        <  2:547443/150‑1 (MQ=255)
                                                                                                               tACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGCGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCgg                                       >  1:329024/1‑150 (MQ=255)
                                                                                                                 ccGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTc                                     <  1:1174887/150‑1 (MQ=255)
                                                                                                                    cTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGCCTGGCTTCGGTCAcc                                  >  1:334095/1‑150 (MQ=255)
                                                                                                                      cccGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCGGGAAAGACTGGCTTCGGTCACCGa                                >  1:1082208/1‑150 (MQ=255)
                                                                                                                       ccGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGAt                               >  2:147597/1‑150 (MQ=255)
                                                                                                                        cGGAAACCACCAGCCCGGTGCGGGCGGCGCGCCAGCAGTGGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAACGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATc                              <  1:870249/150‑1 (MQ=255)
                                                                                                                          gAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCACGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATcgc                            <  1:1025292/150‑1 (MQ=255)
                                                                                                                             accaccAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGATTAACGCTGCGCTGGATAGACTGGCTTCGGTCACCGATCGCGtt                         >  1:1574775/1‑150 (MQ=255)
                                                                                                                              ccaccaGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTc                        >  2:1724186/1‑150 (MQ=255)
                                                                                                                              ccaccaGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTc                        >  2:1392719/1‑150 (MQ=255)
                                                                                                                                accaGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCACCCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGGGAATAACGCTGCGCGGGAACGCCTGGCTGCGGTCCCCGAGCGCGTGCAg                      >  2:1464190/1‑150 (MQ=255)
                                                                                                                                accaGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAg                      >  2:54232/1‑150 (MQ=255)
                                                                                                                                 ccagccagGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGATCCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAgg                     <  1:749456/150‑1 (MQ=255)
                                                                                                                                 ccagccagGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAgg                     <  1:942152/150‑1 (MQ=255)
                                                                                                                                 ccagccagGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGATGGAAATACTGGCTTCGGTCACCGATCGCGTTCAgg                     >  2:89477/1‑150 (MQ=255)
                                                                                                                                  cagccagGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAGAGACTGGCTTCGGTCACCGAGCGCGTTCCGgc                    >  1:1260277/1‑150 (MQ=255)
                                                                                                                                     ccagGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGACACCGATCGCGTTCAGGcgcg                 <  2:502855/150‑1 (MQ=255)
                                                                                                                                      cagGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGt                >  1:618821/1‑150 (MQ=255)
                                                                                                                                       agGTGCTGGCGGCGCGGCAGCAGTGGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTc               <  2:1331291/150‑1 (MQ=255)
                                                                                                                                       aactGCTGGCGCGCCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAGAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTc               >  2:1177251/4‑150 (MQ=255)
                                                                                                                                       aactGCTGGCGCGCCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTc               >  2:1477840/4‑150 (MQ=255)
                                                                                                                                        actGCTGGCGCGCCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTcc              >  1:1067336/3‑150 (MQ=255)
                                                                                                                                         ctGCTGGCGCGCCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTCCg             >  2:65182/2‑150 (MQ=255)
                                                                                                                                         ctGCTGGCGCGCCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTCCg             >  1:905595/2‑150 (MQ=255)
                                                                                                                                           gCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTCCGGt           >  1:73483/1‑150 (MQ=255)
                                                                                                                                            cgggcggcGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAACAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGCCTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTCCGGTg          <  1:727702/148‑1 (MQ=255)
                                                                                                                                            cTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTCCGGTg          <  2:558496/150‑1 (MQ=255)
                                                                                                                                             tGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTCCGGTGc         <  2:1727643/150‑1 (MQ=255)
                                                                                                                                               gcggcgCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTCCGGTGCCa       <  1:919893/150‑1 (MQ=255)
                                                                                                                                                   cgcgcCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTCCGGTGCCATCgg   >  2:475255/1‑150 (MQ=255)
                                                                                                                                                    gcgcCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTCCGGTGCCATCGgc  <  1:659478/150‑1 (MQ=255)
                                                                                                                                                    gcgcCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTCCGGTGCCATCGgc  <  2:1120282/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGCTGCGCTGGAAAGACTGGCTTCGGTCACCGATCGCGTTCAGGCGCGTCCGGTGCCATCGGC  >  NC_000913/493188‑493485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: