Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,906,830 G→A 5.8% intergenic (‑263/+23) bglG ← / ← phoU transcriptional antiterminator of the bgl operon/negative regulator of PhoR/PhoB two‑component regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,906,8300GA 5.8% 494.2 / 11.4 155intergenic (‑263/+23)bglG/phoUtranscriptional antiterminator of the bgl operon/negative regulator of PhoR/PhoB two‑component regulator
Reads supporting (aligned to +/- strand):  ref base G (66/80);  new base A (4/5);  total (70/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.37e-01

Only 100 of 156 total aligned reads displayed.

AACGAACATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGCCAATA  >  NC_000913/3906681‑3906976
                                                                                                                                                     |                                                                                                                                                  
 aCGAACATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGt                                                                                                                                                   >  2:1051658/1‑150 (MQ=255)
 aCGAACATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGt                                                                                                                                                   <  1:1150837/150‑1 (MQ=255)
  cGAACATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGtt                                                                                                                                                  >  1:1702073/1‑150 (MQ=255)
   gAACATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTa                                                                                                                                                 <  2:1690411/150‑1 (MQ=255)
    aaCATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGATATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTAt                                                                                                                                                <  2:131052/150‑1 (MQ=255)
      aaTCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAACTTAATTATTg                                                                                                                                              <  1:169619/149‑1 (MQ=255)
       aTCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGAGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTgg                                                                                                                                             <  1:1675197/150‑1 (MQ=255)
          cAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAACTTAATTATTGGGAt                                                                                                                                          <  2:1601251/150‑1 (MQ=255)
           aGGTTCGGAAATTAATTTAATTGCGGGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGTAATTTATAAAGTTATATATAACAAATCCCAATAACTTAATTATTGGGAtt                                                                                                                                         <  2:313695/150‑1 (MQ=255)
            ggTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGAttt                                                                                                                                        >  1:1593099/1‑150 (MQ=255)
             tttCGGAAATTAATTTAATTTCGTGCAGAGAAAATAGCAATGCGCTAATGATAAAAATATGACCATGCTCGCAGTTATTTACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATACTTAAGTTATTGGGATTTg                                                                                                                                       <  1:1457638/149‑1 (MQ=255)
                  gAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTAGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATACAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTgg                                                                                                                                  <  2:1112776/150‑1 (MQ=255)
                    aattaattTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTg                                                                                                                                <  2:1367282/150‑1 (MQ=255)
                       taatttaattGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAat                                                                                                                             <  1:1528263/150‑1 (MQ=255)
                        aatttaattGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAatt                                                                                                                            >  1:582824/1‑150 (MQ=255)
                        aatttaattGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAatt                                                                                                                            >  2:648714/1‑150 (MQ=255)
                        aatttaattGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAatt                                                                                                                            <  2:346311/150‑1 (MQ=255)
                           ttaattGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAattatt                                                                                                                         <  2:379252/150‑1 (MQ=255)
                            taattGCGTGCAGAGAAAATAGCAATGAGCTTTTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTTAGTTATTGGGATTTGTCTGGTGAATTAttt                                                                                                                        <  2:384665/150‑1 (MQ=255)
                             aattGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTg                                                                                                                       <  1:1066183/150‑1 (MQ=255)
                             aattGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTg                                                                                                                       <  1:647529/150‑1 (MQ=255)
                               ttGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTc                                                                                                                     <  2:1139027/150‑1 (MQ=255)
                                tGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCg                                                                                                                    <  1:792242/150‑1 (MQ=255)
                                 gCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGc                                                                                                                   <  1:1294279/150‑1 (MQ=255)
                                 gCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGc                                                                                                                   >  2:507253/1‑150 (MQ=255)
                                  cGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCt                                                                                                                  >  2:1631519/1‑150 (MQ=255)
                                   gTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTa                                                                                                                 >  1:1238845/1‑150 (MQ=255)
                                     gCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATc                                                                                                               >  1:929261/1‑150 (MQ=255)
                                      cAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCt                                                                                                              >  1:696939/1‑150 (MQ=255)
                                      cAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCt                                                                                                              >  1:978310/1‑150 (MQ=255)
                                            aaaTAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTcccc                                                                                                        <  1:641649/150‑1 (MQ=255)
                                             aaTAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCg                                                                                                       <  2:656450/150‑1 (MQ=255)
                                                 gCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTccc                                                                                                         >  1:382020/1‑144 (MQ=255)
                                                 gCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCcag                                                                                                   <  1:898011/150‑1 (MQ=255)
                                                  aaaTTCGCTATTGATAAAAATATGACCATGCTCGCATTTATTAACTTTGTGTAATTTTAGTAATTTAAAAACTTATATAAAACAAATACCAATAATTAAGTAATTTGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCcagccagc                                                                                                  <  2:1183580/149‑1 (MQ=255)
                                                    aTGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCcagcag                                                                                                <  2:636641/150‑1 (MQ=255)
                                                      gcgcTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGtt                                                                                              >  2:658844/1‑150 (MQ=255)
                                                       cgcTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCAGACAGCAGttt                                                                                             >  1:228922/1‑150 (MQ=255)
                                                        tcTATTGATAAAAATATGAACATGCTCGCAGTTATTAAGTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATATTTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTa                                                                                            <  2:1214511/149‑1 (MQ=255)
                                                        gcTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGAATTTTa                                                                                            >  1:1320966/1‑150 (MQ=255)
                                                           aTTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATcc                                                                                         <  2:338965/150‑1 (MQ=255)
                                                            ttGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCa                                                                                        >  2:1080590/1‑150 (MQ=255)
                                                              gATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGc                                                                                      >  2:627343/1‑150 (MQ=255)
                                                               aTAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCt                                                                                     >  1:388110/1‑150 (MQ=255)
                                                                    aaTATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCg                                                                                <  2:811773/150‑1 (MQ=255)
                                                                      tatGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGcc                                                                              <  1:665763/150‑1 (MQ=255)
                                                                        tGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCAc                                                                            <  2:228517/150‑1 (MQ=255)
                                                                         gACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCAcc                                                                           >  1:1666817/1‑150 (MQ=255)
                                                                           ccATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACAGa                                                                         >  2:1060657/1‑150 (MQ=255)
                                                                               gCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGCCGTg                                                                     <  1:507253/150‑1 (MQ=255)
                                                                               gCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTg                                                                     >  2:361425/1‑150 (MQ=255)
                                                                               gCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTg                                                                     >  2:922115/1‑150 (MQ=255)
                                                                                cTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGa                                                                    >  2:488584/1‑150 (MQ=255)
                                                                                   gCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACgg                                                                 <  2:1016435/150‑1 (MQ=255)
                                                                                    cAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGa                                                                <  1:1445129/150‑1 (MQ=255)
                                                                                           taACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTg                                                         <  2:757128/150‑1 (MQ=255)
                                                                                            aaCTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGc                                                        >  1:1601188/1‑150 (MQ=255)
                                                                                             aCTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGc                                                                                      <  2:473129/119‑1 (MQ=255)
                                                                                             aCTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGcc                                                       <  1:764667/150‑1 (MQ=255)
                                                                                                 tgtgTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCtt                                                   >  1:720334/1‑150 (MQ=255)
                                                                                                  gtgtAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTc                                                  >  1:142973/1‑150 (MQ=255)
                                                                                                  gtgtAATTTTAGGAATTTATAAAGTTATATATAACAAATCACAATAATTAAGTTATTGGGATTTGTCTGTTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCtta                                                  >  1:1072278/1‑149 (MQ=255)
                                                                                                    gtAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCAc                                                >  2:1314941/1‑150 (MQ=255)
                                                                                                    gtAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCAc                                                >  2:1687287/1‑150 (MQ=255)
                                                                                                          ttAGGAATTTATAAAGTTATATTTAAAAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACgtagta                                          <  1:823996/150‑1 (MQ=255)
                                                                                                           tAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGtagtag                                         <  2:590390/150‑1 (MQ=255)
                                                                                                           tAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGtagtag                                         >  2:893157/1‑150 (MQ=255)
                                                                                                             tgAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCAGCGACGTGACGGAAATCCTGCCCCTTCACGTAGTagaa                                       <  1:43894/149‑1 (MQ=255)
                                                                                                             tgAATTTATAAAGTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTAACTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTagaag                                      <  1:1130067/149‑1 (MQ=255)
                                                                                                             ggAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTTCCCCTTCACGTAGTagaa                                       >  1:553726/1‑150 (MQ=255)
                                                                                                              gAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTagaag                                      <  2:696939/150‑1 (MQ=255)
                                                                                                               aaTTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTagaaga                                     <  2:722412/150‑1 (MQ=255)
                                                                                                                 tttATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATa                                   <  1:1080590/150‑1 (MQ=255)
                                                                                                                  ttATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATaa                                  >  1:460473/1‑150 (MQ=255)
                                                                                                                   tataAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGc                                                        >  2:521588/1‑127 (MQ=255)
                                                                                                                   tataAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGc                                                        <  1:521588/127‑1 (MQ=255)
                                                                                                                    ataAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAAc                                >  1:1521167/1‑150 (MQ=255)
                                                                                                                    ataAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAAc                                <  2:458511/150‑1 (MQ=255)
                                                                                                                    ataAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAAc                                >  2:1481011/1‑150 (MQ=255)
                                                                                                                         gTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTcaca                           <  2:1702073/150‑1 (MQ=255)
                                                                                                                          ttATATATAACACATCCAAATAATTAAGTTATTGTGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCATCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACaa                          <  2:1530919/150‑1 (MQ=255)
                                                                                                                            atatataACAAATCCCAATAATTAAGTTATTGGGATTTGTCAGGTGAATTATATGTCGCTATCTTTCCCCGCCAGAATTTTATCCAGATCATAGCCACCGACGAGACGGACATCCTGCCCCTTCACGTAGTAGAAGATAAACTCTCAAat                        <  1:280027/150‑1 (MQ=255)
                                                                                                                               tataACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATTGCCACCGACGTGACGGAAATCCTGCCGCTTCACGTAGAAGAAGATAAACTCACAAATAtt                     >  1:256418/1‑150 (MQ=255)
                                                                                                                                ataACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTc                    >  2:992669/1‑150 (MQ=255)
                                                                                                                                ataACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTc                    >  2:282787/1‑150 (MQ=255)
                                                                                                                                 agaCAAATCCCAATAACTTAATTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAATACCTGCCCCTTCACGTTTTATAATATAAAATCACAAATTTTcg                   >  1:877855/3‑149 (MQ=255)
                                                                                                                                 agaCAAATCCCAATAACTTAATTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCt                   >  1:1424540/3‑150 (MQ=255)
                                                                                                                                  aaCAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTg                  <  2:1666352/150‑1 (MQ=255)
                                                                                                                                  aaCAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTg                  <  2:1593099/150‑1 (MQ=255)
                                                                                                                                  aaCAAATCCCAATAATTAAGTTATTGGGATTTGGCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTg                  <  2:1376036/150‑1 (MQ=255)
                                                                                                                                    cAATTCACAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCTCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCTCGTAGTAGAAGATAAACTCACAAATATTCTGGc                <  1:1051658/150‑1 (MQ=255)
                                                                                                                                    cAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCCCAAATATTCTGGc                <  2:388110/150‑1 (MQ=255)
                                                                                                                                     aaaTCCCAATAACTTAATTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCa               >  1:991246/1‑150 (MQ=255)
                                                                                                                                      aaTCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAg              >  1:788907/1‑150 (MQ=255)
                                                                                                                                      aaTCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAg              >  1:228630/1‑150 (MQ=255)
                                                                                                                                      aaTCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCAAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAg              >  1:652654/1‑150 (MQ=255)
                                                                                                                                       aTCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGc             >  1:1658831/1‑150 (MQ=255)
                                                                                                                                       aTCCCAATAACTTAATTATTGGGATTTGTCTGGTGAATTATTTGTCTCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCAATCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGc             >  1:481761/1‑150 (MQ=255)
                                                                                                                                           cAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCAGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCACCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTc         >  2:881681/1‑150 (MQ=255)
                                                                                                                                              aaatTAAGTAATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCTCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGcc      <  1:291662/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AACGAACATCCAGGTTCGGAAATTAATTTAATTGCGTGCAGAGAAAATAGCAATGCGCTATTGATAAAAATATGACCATGCTCGCAGTTATTAACTTTGTGTAATTTTAGGAATTTATAAAGTTATATATAACAAATCCCAATAATTAAGTTATTGGGATTTGTCTGGTGAATTATTTGTCGCTATCTTTCCCCGCCAGCAGTTTATCCAGCTCATCGCCACCGACGTGACGGAAATCCTGCCCCTTCACGTAGTAGAAGATAAACTCACAAATATTCTGGCAGCGGTCGCCAATA  >  NC_000913/3906681‑3906976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: