Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,915,518 T→G 6.4% intergenic (‑318/+35) glmU ← / ← atpC fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase/F1 sector of membrane‑bound ATP synthase, epsilon subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,915,5180TG 6.4% 301.6 / 6.1 103intergenic (‑318/+35)glmU/atpCfused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase/F1 sector of membrane‑bound ATP synthase, epsilon subunit
Reads supporting (aligned to +/- strand):  ref base T (44/52);  new base G (2/5);  total (46/57)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

AGGCAGTACCTTAATTTAAGCGTCAGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTAATGTGCTCT  >  NC_000913/3915370‑3915664
                                                                                                                                                     |                                                                                                                                                  
aGGCAGTACCTTAATTTAAGCGTCAGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGtt                                                                                                                                                   >  1:1647488/1‑150 (MQ=255)
       aCCTTAATTTAAGCGTCAGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGAc                                                                                                                                            >  1:1714645/1‑150 (MQ=255)
        ccTTAATTTAAGCGTCAGTTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACt                                                                                                                                           <  1:1484019/150‑1 (MQ=255)
         cTTAATTTAAGCGTCAGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTg                                                                                                                                          >  2:1527993/1‑150 (MQ=255)
            aaTTTAAGCGTCAGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCt                                                                                                                                       <  1:996894/150‑1 (MQ=255)
              tttAAGCGTCAGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGTGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGGCACACGCGCAAAAAAAAGCCAGTCTGGAAACAGGCTGGCttt                                                                                                                                     <  2:1462977/150‑1 (MQ=255)
               ttAAGCGTCAGGTGGATGTTTTTGCTCTTATTTCGATCAATTAATAAACAGGAAACCAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGTCTGGAAACAGGCTGGCtttt                                                                                                                                    <  1:1641582/150‑1 (MQ=255)
                tAAGCGTCAGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTtg                                                                                                                                   >  2:492194/1‑150 (MQ=255)
                 aaGCGTCAGGTGGATGTTTTTGCTCTTATTTCTATCAATGAATAAACACGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTtgt                                                                                                                                  <  1:559501/150‑1 (MQ=255)
                  aGCGTCAGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGTCTGGAAACAGGCTGGCtttttt                                                                                                                                  <  2:263374/149‑3 (MQ=255)
                  aGCGTCAGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGTCTGGAAACAGGCTGGCtttttt                                                                                                                                  >  1:263374/1‑147 (MQ=255)
                  aGCGTCAGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTtgtg                                                                                                                                 <  2:847208/150‑1 (MQ=255)
                      tCAGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCttt                                                                                                                             <  2:1393763/150‑1 (MQ=255)
                        aGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTc                                                                                                                           >  1:1299372/1‑150 (MQ=255)
                        aGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTc                                                                                                                           >  1:642572/1‑150 (MQ=255)
                        aGGGGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTAAATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTc                                                                                                                           <  1:1733790/150‑1 (MQ=255)
                           tGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAg                                                                                                                        >  1:780704/1‑150 (MQ=255)
                             gATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGcc                                                                                                                      <  2:1689180/150‑1 (MQ=255)
                               tGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCgg                                                                                                                    >  1:727402/1‑150 (MQ=255)
                               tGTTTTTGCTATTATTTCTATCAATTAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTAAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCgg                                                                                                                    <  2:297981/150‑1 (MQ=255)
                                gTTTTTGCTCTTAATTCGATCAATGAATAAACAGGAAAACAGGGGGGGTTATAATCAGCAGGATCTATGTGAACGCTTTTCAGGACGGTTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGgt                                                                                                                   <  2:297084/150‑1 (MQ=255)
                                   tttGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGtt                                                                                                                <  1:1527993/150‑1 (MQ=255)
                                      gCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACTCTATTCAGGACGGGTCACACGCGCAAAAAAAATCAAGCCTTTATCAAGAATGGCATTTCTGCTTTACAAGCCGGTTTTACa                                                                                                             >  1:275038/1‑150 (MQ=255)
                                       ctctTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACAt                                                                                                            <  1:1305864/150‑1 (MQ=255)
                                        tctTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATc                                                                                                           <  2:454463/150‑1 (MQ=255)
                                         ctTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCg                                                                                                          >  2:1409685/1‑150 (MQ=255)
                                           tATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCt                                                                                                        >  2:629468/1‑150 (MQ=255)
                                             tttCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCttt                                                                                                      >  2:559034/1‑150 (MQ=255)
                                                 gATCAATGAATAAACAGGAAAACAGGGTGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACTGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTg                                                                                                  <  1:375000/150‑1 (MQ=255)
                                                 gATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTg                                                                                                  >  1:448889/1‑150 (MQ=255)
                                                  aTCAATGAATAAACAGGAAAACAGGGGTGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTgg                                                                                                 <  1:972300/150‑1 (MQ=255)
                                                  aTCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTgg                                                                                                 >  2:1294752/1‑150 (MQ=255)
                                                     aaTGTATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGCATCTATGTGACCCCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCa                                                                                              <  2:86351/150‑1 (MQ=255)
                                                     aaTGAATAAACAGGAAAACCGGGGGGTTTTATATCAGGAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCa                                                                                              <  2:721095/150‑1 (MQ=255)
                                                      aTGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGATTTTCAAGCCGGTGTTACATCGCTTTTTTGGTaaa                                                                                             >  1:524749/1‑150 (MQ=255)
                                                       tGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACTTCGCTTTTTTGGTCaaa                                                                                            >  2:235351/1‑149 (MQ=255)
                                                       tGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAAc                                                                                            >  1:173359/1‑150 (MQ=255)
                                                           tAAGCAGGAAAACAGGGGGGTTTTATATCAGCTGGATCTATGTGAACGCTATTCAGGTCGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGACGGTGTTACATAGCTTTTTTGGGCATCTCGa                                                                                        >  2:227274/1‑150 (MQ=255)
                                                            aaaCAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGAt                                                                                       <  2:508353/150‑1 (MQ=255)
                                                            aaaCAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGGTACATCGCTTTTTTGGTCAACTCGAt                                                                                       <  1:101601/150‑1 (MQ=255)
                                                             aaCAGGAAAACAGGTGGGTTTTATATCAGAAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTACAGCATGGCTTTTGTGCTTTTCAAGACGTTGTTACATCGCTTTTTTGGTCAACTCGATa                                                                                      >  1:368990/1‑150 (MQ=255)
                                                               cAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTTTTTCCAGACTGGCTTTTTTGCTTTTCAAGCCGGTGTTACATAGCTTTTTTGGTCAACTCGATAAc                                                                                    >  1:340438/1‑150 (MQ=255)
                                                                 ggAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAAcgc                                                                                  <  1:805354/150‑1 (MQ=255)
                                                                    aaaCAGGGGGGTTTTTAATGATCATGTTCTATTGTCACGGTATTTCTGTCGGGGCACACGGCGAAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACgcgc                                                                                <  1:1411515/150‑1 (MQ=255)
                                                                           ggggTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTgcgc                                                                        <  2:448889/150‑1 (MQ=255)
                                                                           ggggTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGGCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTgcgc                                                                        <  1:149631/150‑1 (MQ=255)
                                                                              gTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGAt                                                                     <  1:1436072/150‑1 (MQ=255)
                                                                              gTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGAGCGAt                                                                     >  1:1306878/1‑150 (MQ=255)
                                                                               gtgtataTCAGCAGGCTCTATGTGATCGCTATTCAGGACGGGTCACACGCGCAAAAACCAGCCCGCCTCGTTCCAGACTGGCGTTTGTGCTGTTCCAGCCGGTGTTACAGCGCTTTTTTGGTCAACTCGCTCCCGCGCAGCTGCGCGATc                                                                    <  1:74290/147‑1 (MQ=255)
                                                                                  tataTCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATATCGCGCAGCTGcgcgttatcg                                                                 <  1:460107/150‑7 (MQ=255)
                                                                                  tataTCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCt                                                                 >  1:886363/1‑150 (MQ=255)
                                                                                   atatCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCtt                                                                <  1:367528/150‑1 (MQ=255)
                                                                                     atCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGCTCGCTTTg                                                              >  2:1710527/1‑150 (MQ=255)
                                                                                     atCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTg                                                              <  2:117504/150‑1 (MQ=255)
                                                                                     atCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTg                                                              <  2:544976/150‑1 (MQ=255)
                                                                                     atCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTAGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTg                                                              <  1:1317357/150‑1 (MQ=255)
                                                                                     atCAGCAGGATCTATGTGAACGCTATTCAGGACGGGGCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTg                                                              <  1:961063/150‑1 (MQ=255)
                                                                                       cagcagGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGc                                                            >  2:383/1‑150 (MQ=255)
                                                                                        agcagGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGcc                                                           <  2:1707834/150‑1 (MQ=255)
                                                                                        agcagGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGcc                                                           >  2:877759/1‑150 (MQ=255)
                                                                                        agcagGATCTATGTGAACGCTAGTCAGGCCGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTACCAGAATGGCTTATGTGCTTTTAAAGCCGGGGTTCCCTCGCTGATTTGGTCAACTCGATAACGCGCTGCTGAGCCATCGCGTTGGcc                                                           >  2:1144680/1‑150 (MQ=255)
                                                                                         gcagGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCa                                                          >  2:370774/1‑150 (MQ=255)
                                                                                          cagGATCTATGTGAACGCTCTTCAGGACGGGTCACACGCTCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAg                                                         <  1:1078785/150‑1 (MQ=255)
                                                                                           agGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTATGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGt                                                        <  1:237209/150‑1 (MQ=255)
                                                                                            ggATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGtt                                                       >  1:47243/1‑150 (MQ=255)
                                                                                            ggATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGtt                                                       >  1:246831/1‑150 (MQ=255)
                                                                                             gagcaaTGTGAACGCTATTCAGGACGGGTCACACGCGCAATAAAAAGCCAGCCTGTTTCCAGACTGGCTATTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTc                                                      <  1:298906/145‑1 (MQ=255)
                                                                                                cTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGc                                                   <  1:657272/150‑1 (MQ=255)
                                                                                                cTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGc                                                   <  1:1008852/150‑1 (MQ=255)
                                                                                                cTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGc                                                   <  2:727402/150‑1 (MQ=255)
                                                                                                  aTGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAg                                                 <  1:1691106/150‑1 (MQ=255)
                                                                                                  aTGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAg                                                 >  2:377518/1‑150 (MQ=255)
                                                                                                     tgAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGCCTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACg                                              >  1:1013159/1‑150 (MQ=255)
                                                                                                      gAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGc                                             >  1:1725390/1‑150 (MQ=255)
                                                                                                       aaCGCTATTCAGGACGGGTCGCACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGcc                                            >  2:189680/1‑150 (MQ=255)
                                                                                                       aaCGCTATTCAGGACGGGTCGCACGCGCAAAAAAAAGCCAGCCTGTTACCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATAGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGcc                                            >  2:189677/1‑150 (MQ=255)
                                                                                                        aagCTATTCAGGACGGGTAACACGCTCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCt                                           <  2:1530793/148‑1 (MQ=255)
                                                                                                               tCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTa                                    >  2:685696/1‑150 (MQ=255)
                                                                                                                cAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTaa                                   >  2:1123148/1‑150 (MQ=255)
                                                                                                                cAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTaa                                   >  2:1321357/1‑150 (MQ=255)
                                                                                                                cAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTaa                                   >  1:748153/1‑150 (MQ=255)
                                                                                                                    aCGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACAGCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTa                               <  1:1251117/150‑1 (MQ=255)
                                                                                                                       ggTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGt                            >  2:1258823/1‑150 (MQ=255)
                                                                                                                           acacGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTAAATCCCATTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGcc                        >  2:1180782/1‑150 (MQ=255)
                                                                                                                              cgcgCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGAAAACGCGCAGCGGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTg                     <  1:272281/150‑1 (MQ=255)
                                                                                                                                 cgcAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTgag                   <  1:1207268/150‑1 (MQ=255)
                                                                                                                                   cAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGagag                 <  2:1189579/150‑1 (MQ=255)
                                                                                                                                   cAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGagag                 >  2:506730/1‑150 (MQ=255)
                                                                                                                                     aaaaaaaGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAgct               >  2:453759/1‑150 (MQ=255)
                                                                                                                                        aaaaGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAgctgct            <  2:1299372/150‑1 (MQ=255)
                                                                                                                                        aaaaGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAgctgct            <  1:1409685/150‑1 (MQ=255)
                                                                                                                                        aaaaGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAgctgct            <  2:1647488/150‑1 (MQ=255)
                                                                                                                                         aaaGCCAGTCTGGAAACAGGCTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATAGCTTTGGCCAGTTCCGCAGAAGCCTGAGCGTAATCTACGACGCCGTGAGAGCTGCTa           >  1:751760/1‑150 (MQ=255)
                                                                                                                                          aaGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTATCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTaa          <  2:106107/150‑1 (MQ=255)
                                                                                                                                           agccagccTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTAAt         >  2:1192980/1‑150 (MQ=255)
                                                                                                                                           agccagcaTTTTTCCAGACTGGCTGTTGTGATTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAAAGCGCACCTGCTCGCTCGCTTTGGCCAGTTCAGCAGAAGGCTGAGCGTAATCTACGTCGCAGTGAGAGCTGCTAAt         >  1:111673/1‑150 (MQ=255)
                                                                                                                                               agccTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTAATGTGc     <  2:944540/150‑1 (MQ=255)
                                                                                                                                               agccTGGTTCGAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTAATGTGc     <  2:277966/150‑1 (MQ=255)
                                                                                                                                                 ccTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTAATGTGctc   <  1:370774/150‑1 (MQ=255)
                                                                                                                                                  cTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTAATGTGctct  <  1:1382386/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AGGCAGTACCTTAATTTAAGCGTCAGGTGGATGTTTTTGCTCTTATTTCGATCAATGAATAAACAGGAAAACAGGGGGGTTTTATATCAGCAGGATCTATGTGAACGCTATTCAGGACGGGTCACACGCGCAAAAAAAAGCCAGCCTGTTTCCAGACTGGCTTTTGTGCTTTTCAAGCCGGTGTTACATCGCTTTTTTGGTCAACTCGATAACGCGCAGCTGCGCGATCGCTTTGGCCAGTTCCGCAGACGCCTGAGCGTAATCTACGTCGCCGTGAGAGCTGCTAATGTGCTCT  >  NC_000913/3915370‑3915664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: