Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,918,304 A→G 5.1% intergenic (‑39/+12) atpG ← / ← atpA F1 sector of membrane‑bound ATP synthase, gamma subunit/F1 sector of membrane‑bound ATP synthase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,918,3040AG 5.1% 398.9 / 8.3 137intergenic (‑39/+12)atpG/atpAF1 sector of membrane‑bound ATP synthase, gamma subunit/F1 sector of membrane‑bound ATP synthase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (68/61);  new base G (5/2);  total (74/63)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.98e-01

Only 100 of 137 total aligned reads displayed.

TGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAG  >  NC_000913/3918159‑3918449
                                                                                                                                                 |                                                                                                                                                 
  cGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAgccgcc                                                                                                                                             <  1:920460/150‑1 (MQ=255)
       tACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACg                                                                                                                                        >  1:83356/1‑150 (MQ=255)
        aCGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGGCCGCAAggcat                                                                                                                                       <  2:410044/150‑5 (MQ=255)
            aTTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTAcc                                                                                                                                   <  2:1682383/150‑1 (MQ=255)
               ttGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCGTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTAGCGGCCTGCCCTAAGGCACGCCGCCAGCCGTTACCaag                                                                                                                                >  2:85253/1‑148 (MQ=255)
                 ggAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGAt                                                                                                                              >  2:1482035/1‑150 (MQ=255)
                 ggAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGACGCCAGACGTTACCAGGAt                                                                                                                              >  2:568504/1‑150 (MQ=255)
                  gAAGCGGCGACCATCTGCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGGTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGACCTTAGGCAAGCCGCCAGACGTTACCAGGAtt                                                                                                                             <  1:1053893/150‑1 (MQ=255)
                     gcggcgACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTTCCAGGATTggg                                                                                                                          <  1:941349/150‑1 (MQ=255)
                      cggcgACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGt                                                                                                                         >  1:207134/1‑150 (MQ=255)
                       ggcgACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGtt                                                                                                                        >  2:389130/1‑150 (MQ=255)
                         cgACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGc                                                                                                                      <  1:568504/150‑1 (MQ=255)
                         cgACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGc                                                                                                                      >  2:716705/1‑150 (MQ=255)
                         cgACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGATTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGc                                                                                                                      <  1:219373/150‑1 (MQ=255)
                            ccATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCttt                                                                                                                   >  1:1486729/1‑150 (MQ=255)
                                ctcCATCGCTTTAGTGCTCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAg                                                                                                               <  2:651514/150‑1 (MQ=255)
                                ctcCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGCGCTTCTCCTCAATGCCTTGCGGACTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGAATGGGTTGCTTTGAAg                                                                                                               >  1:1055319/1‑150 (MQ=255)
                                ctcCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAg                                                                                                               <  2:1637320/150‑1 (MQ=255)
                                ctcCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAg                                                                                                               <  1:146341/150‑1 (MQ=255)
                                        cTTTAGGGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAg                                                                                                       <  1:1039976/150‑1 (MQ=255)
                                         tttAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAgg                                                                                                      <  1:1222968/150‑1 (MQ=255)
                                              tgttCTTTTGCGTGTTCTGGAGGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGcc                                                                                                 <  2:831324/147‑1 (MQ=255)
                                              tGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGcc                                                                                                 <  1:1596459/150‑1 (MQ=255)
                                               gATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCt                                                                                                >  1:1049397/1‑150 (MQ=255)
                                               gATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCt                                                                                                >  2:1318604/1‑150 (MQ=255)
                                               gATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGCCAAGCCGCAAGACCTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCt                                                                                                >  2:622746/1‑150 (MQ=255)
                                                  cTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTc                                                                                             <  2:586548/150‑1 (MQ=255)
                                                  cTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTc                                                                                             <  2:384879/150‑1 (MQ=255)
                                                   ttttGCGTGGGCTGGACTCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCa                                                                                            <  2:118677/150‑1 (MQ=255)
                                                     ttGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGc                                                                                          <  2:198686/150‑1 (MQ=255)
                                                      tGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCt                                                                                         >  1:1340852/1‑150 (MQ=255)
                                                        cGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTg                                                                                       <  2:41020/150‑1 (MQ=255)
                                                          tgtTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGcc                                                                                     <  1:1464476/150‑1 (MQ=255)
                                                          tgtTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGcc                                                                                     <  2:1350280/150‑1 (MQ=255)
                                                             tCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTc                                                                                  <  1:884105/150‑1 (MQ=255)
                                                                   cgcgTGCGATCTTACTAAGTATCTCTTTTGCGCCGGCCAAGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtc                                                                            <  1:323657/150‑1 (MQ=255)
                                                                   cGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTACTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtc                                                                            <  2:1499474/150‑1 (MQ=255)
                                                                     cTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtcgt                                                                          >  2:54628/1‑150 (MQ=255)
                                                                       tGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtcgtcg                                                                        <  2:1564283/150‑1 (MQ=255)
                                                                            tCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTa                                                                   <  1:885016/150‑1 (MQ=255)
                                                                             cTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAg                                                                  <  2:929643/150‑1 (MQ=255)
                                                                              ttACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGc                                                                 >  1:757579/1‑150 (MQ=255)
                                                                                 ctacGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGCCGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCCGCTTGCCTTCGATTTCGTCGTTGTAGCCAc                                                              >  1:536440/1‑150 (MQ=255)
                                                                                  ttcGTATCTCTTTTGCGCCGGCCATCAGCGTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCAcc                                                             <  2:76108/148‑1 (MQ=255)
                                                                                      tATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCTCCGGTc                                                         >  2:430886/1‑150 (MQ=255)
                                                                                       aTCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCt                                                        >  1:1362830/1‑150 (MQ=255)
                                                                                       aTCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCAGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTTGGTTGCTTTTAAGGAATCGAGGATGCCTTTGAGCTTGCCGTAGTTTTAGTCGTTGTACCCAACGGTCt                                                        >  2:1516373/1‑150 (MQ=255)
                                                                                         ctctTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTgg                                                      <  1:1125138/150‑1 (MQ=255)
                                                                                         ctctTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTgg                                                      >  1:957914/1‑150 (MQ=255)
                                                                                          tctTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCGTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGt                                                     <  1:203232/150‑1 (MQ=255)
                                                                                           ctTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTTGTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGtt                                                    >  1:1557016/1‑150 (MQ=255)
                                                                                           ctTTTGCGCCGGCCATGAGCTTCCCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGtt                                                    <  1:727845/150‑1 (MQ=255)
                                                                                            ttttGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTg                                                   <  2:1605436/150‑1 (MQ=255)
                                                                                             tttGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGa                                                  >  1:729867/1‑150 (MQ=255)
                                                                                               tGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGAtc                                                <  1:788623/150‑1 (MQ=255)
                                                                                               tGCGCCGGCCATGAGCGTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTTCCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGAtc                                                <  2:1140518/150‑1 (MQ=255)
                                                                                                gcgcCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGAtct                                               >  2:26719/1‑150 (MQ=255)
                                                                                                   ccGGCCATGAGCTTCTCCTCATTGCGTTGCGGCCTGCCCTATGGCAAGCCGCCAGACGTTACCAGGCTTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCtt                                            <  2:1055319/150‑1 (MQ=255)
                                                                                                    cGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTg                                           <  2:946665/150‑1 (MQ=255)
                                                                                                       ccATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTATGAAGGAATCGAGGATGCCTATCAGCTAGCCTTCGATTTCGTCGTTGTAGACACCGGTCTGGTTGCTCTCTTGCAt                                        >  1:264300/1‑150 (MQ=255)
                                                                                                        cATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATc                                       >  1:1037293/1‑150 (MQ=255)
                                                                                                        cATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATc                                       >  2:1050484/1‑150 (MQ=255)
                                                                                                        cATGAGCTGCTCCTCATTGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGGTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATc                                       <  2:1147222/150‑1 (MQ=255)
                                                                                                         aTGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGAAGTTACCAGGATTGGGTTGCTTTGAAGGAATAGAGGATGCCTTTCATATTGCCTTCGATTTAGTCGTTGTAGCCACCGGTCAGGTTGATCTCTTtcatca                                      >  1:1712754/1‑150 (MQ=255)
                                                                                                          tGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCaa                                     >  1:43533/1‑150 (MQ=255)
                                                                                                               ttCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAg                                <  2:808464/150‑1 (MQ=255)
                                                                                                                tctcCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGc                               >  1:123883/1‑150 (MQ=255)
                                                                                                                   cctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGACTCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCCGGTTGATCTCTTGCATCAACGGAGCGTg                            <  1:412098/150‑1 (MQ=255)
                                                                                                                   cctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGTGGATGCCTTTCAGCTTGCCTTCGTTTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTg                            <  2:895310/150‑1 (MQ=255)
                                                                                                                   cctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTg                            >  1:368308/1‑150 (MQ=255)
                                                                                                                   cctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCGTTGAAGGAATCGAGGATGCCTATCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGGCTGGTTGATCTCTTGCATCAACGGAGCGTg                            <  2:1237460/150‑1 (MQ=255)
                                                                                                                     tcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGAt                          <  1:971941/150‑1 (MQ=255)
                                                                                                                      cAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGCGGATGCCTTTCAGCTTGCCTGCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGAGCTCTTGAATCAACGGAGCGTGATc                         >  2:1132639/1‑150 (MQ=255)
                                                                                                                      cAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATc                         <  2:43533/150‑1 (MQ=255)
                                                                                                                      cAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATc                         >  2:609878/1‑150 (MQ=255)
                                                                                                                      cAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCATCGAGTTCGTCGTTGTAGCCCGCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATc                         >  2:1138459/1‑150 (MQ=255)
                                                                                                                       aaTGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGCCGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATTCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCa                        >  2:720559/1‑150 (MQ=255)
                                                                                                                       aaTGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCa                        <  2:1049397/150‑1 (MQ=255)
                                                                                                                          gCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACgg                     >  2:370264/1‑150 (MQ=255)
                                                                                                                          gCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACgg                     <  1:478686/150‑1 (MQ=255)
                                                                                                                           ccTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGt                    <  2:868819/150‑1 (MQ=255)
                                                                                                                            cTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTc                   <  1:1712414/150‑1 (MQ=255)
                                                                                                                             tggCGGCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGATGATCTCTTGCATCAACGGAGCGTGATCACGGTCg                  >  1:697803/3‑150 (MQ=255)
                                                                                                                              ggCGGCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCgc                 >  1:614309/2‑149 (MQ=255)
                                                                                                                                 ggCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCCCCGGTCTGGTTGATCTCTTGCTTCAACGGAGCGTGATCACGGTCGACGt              >  1:1155342/1‑150 (MQ=255)
                                                                                                                                 ggCTTGCCTTAGGGCAGGCCGCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTa             >  2:193665/1‑150 (MQ=255)
                                                                                                                                  gCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTa             >  1:1179304/1‑150 (MQ=255)
                                                                                                                                      gCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGGCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGcc         <  1:1547943/150‑1 (MQ=255)
                                                                                                                                       cccTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCca        >  2:397093/1‑150 (MQ=255)
                                                                                                                                        ccTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCcag       >  1:1415787/1‑150 (MQ=255)
                                                                                                                                        ccTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCcag       >  1:939475/1‑150 (MQ=255)
                                                                                                                                         ctccggCAAGCCGCCAGACGTGACCCGGATGGGGTTGCTTTGAAGGACTCGAGGCGGCCTGTCTGCTTGCCGTCGATGGCGGCGTTGTAGCCTCCGGGCTGGGTGATCTCTTGCATCAACGGAGCGTGATGACGGTCGACGTAAGCcagc      <  1:1204076/146‑1 (MQ=255)
                                                                                                                                         cTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCcagc      <  1:1318604/150‑1 (MQ=255)
                                                                                                                                         cTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCcagc      <  1:992914/150‑1 (MQ=255)
                                                                                                                                         cTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCcagc      >  2:1173472/1‑150 (MQ=255)
                                                                                                                                           aaGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCAGCAACGGCGCGTGATCACGGTCGACGTACGCcaggag    >  2:114588/1‑150 (MQ=255)
                                                                                                                                            aGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCACCGGAGCGTGATCACGGTCGACGTAAGCCAGCaga   >  2:158149/1‑150 (MQ=255)
                                                                                                                                            aGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATATCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGCATCGTCATCACGGACGACGTAAGCCAGCaga   >  1:273506/1‑150 (MQ=255)
                                                                                                                                             ggCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCagaa  >  1:1405217/1‑149 (MQ=255)
                                                                                                                                             ggCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCagaa  >  1:1412090/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TGCGATTTACGCATTTTGGAAGCGGCGACCATCTCCATCGCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGTTGATCTCTTGCATCAACGGAGCGTGATCACGGTCGACGTAAGCCAGCAGAG  >  NC_000913/3918159‑3918449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: