Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,918,916 T→A 5.8% K314N (AAA→AAT) ‡ atpA ← F1 sector of membrane‑bound ATP synthase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,918,9160TA 5.8% 320.4 / 4.7 114K314N (AAA→AAT) ‡atpAF1 sector of membrane‑bound ATP synthase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (47/60);  new base A (3/4);  total (50/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

TACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACAGCCTGTTT  >  NC_000913/3918768‑3919065
                                                                                                                                                    |                                                                                                                                                     
  ccGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTg                                                                                                                                                    >  2:480325/1‑150 (MQ=255)
   cGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTgg                                                                                                                                                   <  1:711237/150‑1 (MQ=255)
   cGGCGTTGAACAGGTGGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTgg                                                                                                                                                   <  1:1652005/150‑1 (MQ=255)
      cGTGGAACAGGTGGGTTTCGAGTAAGATCTGACCATCGGTAATGGAGTTTAAGTTGGTCGGAACGAACGCAGAAACGTCACACGCCTGTGTTTTGTTAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGa                                                                                                                                                <  2:269788/150‑1 (MQ=255)
        ttGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAg                                                                                                                                              <  1:1630778/150‑1 (MQ=255)
        ttGAACAGGTTGGTGTCCACGAAGAGCTGACCATCGGTAATGGAGATAACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAg                                                                                                                                              <  1:44384/150‑1 (MQ=255)
         tGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAgg                                                                                                                                             >  1:428246/1‑150 (MQ=255)
         tGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAgg                                                                                                                                             <  1:1220746/150‑1 (MQ=255)
         tGAACAGGTTGGTTTCCAGGAAGAGCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCAAAGGTGAAgt                                                                                                                                             <  1:73862/150‑2 (MQ=255)
          gAACAGTTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGGCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCAAAGGTGAAgtg                                                                                                                                            <  1:969621/150‑3 (MQ=255)
          gAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGc                                                                                                                                            >  2:981967/1‑150 (MQ=255)
          gAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGc                                                                                                                                            >  2:59114/1‑150 (MQ=255)
           aaCAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGTAGATTACGTTGGTCGGAACTAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCt                                                                                                                                           <  2:550101/150‑1 (MQ=255)
           aaCAGGGTGGTTTCCAGGAAGATCTGACCATAGGTAATGGAGATTACGATGGTCGGAACGAACGCAGAAACGACACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCt                                                                                                                                           <  1:1093077/150‑1 (MQ=255)
             cAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGaa                                                                                                                                               <  2:1709343/144‑1 (MQ=255)
             cAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTc                                                                                                                                         <  1:944594/150‑1 (MQ=255)
             cAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACACGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGaa                                                                                                                                               >  1:1709343/1‑144 (MQ=255)
             cAGGGTGGGTACCTGGATGATCTGACCCTCGGTATTGGAGATTACGTTGGTCGGAACGACCGCAGAAACGTCCCCCGCCTGAGTTTCGATCATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTc                                                                                                                                         <  1:1123936/150‑1 (MQ=255)
              aGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGCGAAGGCTTCa                                                                                                                                        <  1:1628014/150‑1 (MQ=255)
               ggttggttTCCAGGAAGATCTGACCATCGGTAATGGAGAATACGTTGGTCGGAACGAACGCAGAAAAGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCAGCTTTGGTGAAGGCTTCaa                                                                                                                                       >  1:1118439/1‑150 (MQ=255)
                 ttggttTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACg                                                                                                                                     <  1:1696912/150‑1 (MQ=255)
                     tttCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTAtt                                                                                                                                 <  2:723291/150‑1 (MQ=255)
                        ccAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCgg                                                                                                                              >  2:413796/1‑150 (MQ=255)
                         cAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCCCCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGc                                                                                                                             >  2:1367048/1‑150 (MQ=255)
                         cAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTTGTGAAGGCTTCAACGTATTCGGc                                                                                                                             <  2:715570/150‑1 (MQ=255)
                           ggAAGATCTGACCATCGGTTATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGt                                                                                                                           <  1:1570579/150‑1 (MQ=255)
                            gAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACTAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGtt                                                                                                                          <  2:808689/150‑1 (MQ=255)
                            gAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGtt                                                                                                                          <  2:1629251/150‑1 (MQ=255)
                                aTCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAaca                                                                                                                      >  1:824748/1‑150 (MQ=255)
                                 tCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAacac                                                                                                                     >  2:664645/1‑150 (MQ=255)
                                    gACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCTGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTg                                                                                                                  >  1:337062/1‑150 (MQ=255)
                                    gACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTg                                                                                                                  <  2:178262/150‑1 (MQ=255)
                                     aCCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTgc                                                                                                                 <  2:524506/150‑1 (MQ=255)
                                       cATCGGTAATGGAGATTACGTTGGTCGGAACGATCGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTgcag                                                                                                               <  2:1429692/150‑1 (MQ=255)
                                       cATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTgcag                                                                                                               <  1:1668350/150‑1 (MQ=255)
                                           ggTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACg                                                                                                           >  2:466492/1‑150 (MQ=255)
                                           ggTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTATCACTTCACCTTAGGTGAAGTCTTCAACGTATTCGGCGTTAACACGTGCAGCAcc                                                                                                           >  2:849896/1‑149 (MQ=255)
                                           ggTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCAGCCTCAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCATTCACTTCACCTTTGGTGAATGCTTCAACGTATTCGCCGTTAACACGTGCAGCAcc                                                                                                           >  1:423472/1‑149 (MQ=255)
                                               aTGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTcc                                                                                                       <  2:762703/150‑1 (MQ=255)
                                                  gagaTTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCcagc                                                                                                    <  2:227181/150‑1 (MQ=255)
                                                    gaTTACGTTGGGCGGAACGAACGCAGAAACGTCACCCGCCCGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCcagcag                                                                                                  <  2:250706/150‑1 (MQ=255)
                                                      ttACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGAc                                                                                                <  1:403415/150‑1 (MQ=255)
                                                      ttACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGAc                                                                                                <  2:574898/150‑1 (MQ=255)
                                                         cGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACgag                                                                                             >  2:424155/1‑150 (MQ=255)
                                                         cGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACgag                                                                                             <  1:443606/150‑1 (MQ=255)
                                                           ttGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCACCGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGagag                                                                                           >  1:1584129/1‑150 (MQ=255)
                                                                cGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAg                                                                                      <  1:1211013/150‑1 (MQ=255)
                                                                cGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAg                                                                                      >  2:413620/1‑150 (MQ=255)
                                                                   aacgaacgAAGAAACGTCACCCGCCTGAGTTTCTATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTa                                                                                   <  1:535335/150‑1 (MQ=255)
                                                                     cgaacgCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGCACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGGAGTCGGCGTTAACACGGGCCGCACGCTCCAGCAGCCGAGAGTGGAGGTAgt                                                                                 >  2:1093045/1‑149 (MQ=255)
                                                                     cgaacgCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGa                                                                                 <  1:413796/150‑1 (MQ=255)
                                                                     cgaacgCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACATTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACACTCCAGCAGACGAGAGTGGAGGTAGa                                                                                 >  1:1091022/1‑150 (MQ=255)
                                                                       aacgCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGaaa                                                                               >  1:991008/1‑150 (MQ=255)
                                                                       aacgCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGaaa                                                                               <  1:628367/150‑1 (MQ=255)
                                                                       aacgCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGaaa                                                                               >  1:984222/1‑150 (MQ=255)
                                                                        acgCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGaaaa                                                                              <  1:773851/150‑1 (MQ=255)
                                                                         cgCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAATGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTTGTAAAc                                                                             >  2:240862/1‑150 (MQ=255)
                                                                           cAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGt                                                                           >  2:1125606/1‑150 (MQ=255)
                                                                                aCGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTCTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGccc                                                                      <  1:8535/150‑1 (MQ=255)
                                                                                  gTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCgg                                                                    >  1:315016/1‑150 (MQ=255)
                                                                                    cACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGa                                                                  <  2:1355132/150‑1 (MQ=255)
                                                                                             gAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCAc                                                         <  1:462475/150‑1 (MQ=255)
                                                                                              aGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACg                                                        >  2:1180432/1‑150 (MQ=255)
                                                                                              aGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACg                                                        >  2:1334788/1‑150 (MQ=255)
                                                                                               gTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGt                                                       >  1:10239/1‑150 (MQ=255)
                                                                                               gTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGt                                                       >  2:501527/1‑150 (MQ=255)
                                                                                                     aTAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGc                                                 >  1:1461434/1‑150 (MQ=255)
                                                                                                      tAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCATCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCg                                                <  1:54520/150‑1 (MQ=255)
                                                                                                       aaTCGGCAGTGCGGTCAGAGAACCTGTTTTCCCATTCACTTCAACTTTGGTGAAGGCTGCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCgg                                               <  2:840201/150‑1 (MQ=255)
                                                                                                       aaTCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCgg                                               <  1:981967/150‑1 (MQ=255)
                                                                                                          cGGCCGTGCGGTCAGAGACCCGGGTATCCCTTTCACTTCACCTTTGGTGAAGGCTGCAACGTATTCGGCGATAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGGAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGgacg                                            <  2:623570/150‑1 (MQ=255)
                                                                                                          cGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGgacg                                            <  2:1232628/150‑1 (MQ=255)
                                                                                                          cGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGgacg                                            <  2:1348708/150‑1 (MQ=255)
                                                                                                              cgTGCGGTCAGAGAACCGGTGTTCCCTTTCCCTGCACCTTTGGTGAAGGCTTCAACGTATTCGGCGGAAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACgg                                        <  1:862844/149‑1 (MQ=255)
                                                                                                              aGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACgg                                        <  1:1169845/150‑1 (MQ=255)
                                                                                                              aGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACgg                                        <  2:824748/150‑1 (MQ=255)
                                                                                                               gTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGa                                       >  2:1706063/1‑150 (MQ=255)
                                                                                                                    gTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCCCGCTCCAGCAGACGAGAGTGGAGGTAGACAAAGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGagcagc                                  >  2:481926/1‑150 (MQ=255)
                                                                                                                    gTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGagcagc                                  >  2:1251059/1‑150 (MQ=255)
                                                                                                                     gcAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAgcagca                                 <  1:11261/149‑1 (MQ=255)
                                                                                                                      cAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGATAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGcagcag                                >  1:820361/1‑150 (MQ=255)
                                                                                                                      cAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGcagcag                                >  2:691040/1‑150 (MQ=255)
                                                                                                                             ccGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGGCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATc                         <  1:1367048/150‑1 (MQ=255)
                                                                                                                              cGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTGAACGTATTCGGCGCTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCt                        <  2:267460/150‑1 (MQ=255)
                                                                                                                               ggTGTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGGGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTg                       <  2:1635268/150‑1 (MQ=255)
                                                                                                                                 ttttCCCTTTCCCTGCCCCTTTGGGGACGGCTGCACCGTATGCGGCGTTACCCCGGGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGACAACGGCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGAc                     <  1:1355703/150‑1 (MQ=255)
                                                                                                                                  tttCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACg                    <  1:59114/150‑1 (MQ=255)
                                                                                                                                   ttCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGCCGACGGAGCAGCAGGGAGATCTGACgg                   >  1:712639/1‑150 (MQ=255)
                                                                                                                                    acccTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGt                  <  2:1729195/149‑1 (MQ=255)
                                                                                                                                      ccTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGACCGGGAATGCTTCACGTCCTGGCGGACGACGGAGAAGCAGGGAGATCTGACGGTaa                >  1:1083451/1‑150 (MQ=255)
                                                                                                                                          agccttcacCAAAGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCaa            >  2:190374/4‑150 (MQ=255)
                                                                                                                                           cccttcacCAAAGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAAc           >  1:1311718/3‑150 (MQ=255)
                                                                                                                                            ccttcacCAAAGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAAcc          >  2:1156750/2‑149 (MQ=255)
                                                                                                                                            acttcacCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACa          <  1:472967/150‑1 (MQ=255)
                                                                                                                                            acttcacCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACa          >  1:620277/1‑150 (MQ=255)
                                                                                                                                             cttcacCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCCCGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACAg         >  2:387005/1‑150 (MQ=255)
                                                                                                                                             cttcacCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGCTCTGACGGTAAGCAACAg         >  2:466498/1‑150 (MQ=255)
                                                                                                                                               tcacCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACAGcc       <  1:1693804/150‑1 (MQ=255)
                                                                                                                                                cacCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACAGccg      >  2:1498221/1‑149 (MQ=255)
                                                                                                                                                    tttGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACAGCCTGttt  <  2:32345/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TACCGGCGTTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGACGGTAAGCAACAGCCTGTTT  >  NC_000913/3918768‑3919065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: