Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,923,718 G→C 7.3% intergenic (‑38/+26) rsmG ← / ← mnmG 16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein/5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,923,7180GC 7.3% 452.2 / 20.8 149intergenic (‑38/+26)rsmG/mnmG16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein/5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
Reads supporting (aligned to +/- strand):  ref base G (65/73);  new base C (6/5);  total (71/78)
Fisher's exact test for biased strand distribution p-value = 7.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.33e-01

Only 100 of 150 total aligned reads displayed.

ACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTA  >  NC_000913/3923569‑3923864
                                                                                                                                                     |                                                                                                                                                  
aCGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCgg                                                                                                                                                    >  1:543481/1‑150 (MQ=255)
  gCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGt                                                                                                                                                  >  2:1534973/1‑150 (MQ=255)
   cTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGAGCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTg                                                                                                                                                 <  1:1571175/150‑1 (MQ=255)
    tttGTTCCATTTATGCAGCATATTCACGTGGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGGCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGc                                                                                                                                                <  2:326803/150‑1 (MQ=255)
     ttGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCt                                                                                                                                               >  2:1053627/1‑150 (MQ=255)
          ccATTTATGCAGCATATTCACGTAGGCAATAAACTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCa                                                                                                                                          <  1:828445/150‑1 (MQ=255)
           cATTTCTGCAGCATATTCCCGGAGGCAATCAGCTGGGTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCCGTAAGGAGAGGTGGTTGAGCACCGTGATTACCTGGTCTTGATGCGTTGCCTGGTAAGCACCCGCTTACCAg                                                                                                                                         <  1:1060959/150‑1 (MQ=255)
           cATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAg                                                                                                                                         <  1:69615/150‑1 (MQ=255)
                  gcagcaTATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCAtttt                                                                                                                                  >  2:160660/1‑150 (MQ=255)
                  gcagcaTATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCAtttt                                                                                                                                  >  1:399109/1‑150 (MQ=255)
                   cagcaTATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCAttttt                                                                                                                                 <  2:1693647/150‑1 (MQ=255)
                    cgcaTATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTa                                                                                                                                <  1:914743/149‑1 (MQ=255)
                         atTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCg                                                                                                                           >  2:628165/1‑150 (MQ=255)
                         atTCACGTAGGCAATAAGCTGGGTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCg                                                                                                                           <  2:1256652/150‑1 (MQ=255)
                          ttCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGt                                                                                                                          <  1:1115866/150‑1 (MQ=255)
                           tCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGtt                                                                                                                         <  2:447188/150‑1 (MQ=255)
                                tAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATtcg                                                                                                                    >  1:1299611/1‑150 (MQ=255)
                                tAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATgcg                                                                                                                    >  1:829986/1‑150 (MQ=255)
                                   gCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCTTTATGCGCTa                                                                                                                 >  1:157973/1‑150 (MQ=255)
                                    cAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTac                                                                                                                <  1:160660/150‑1 (MQ=255)
                                        aaGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTacgacg                                                                                                            >  1:45879/1‑150 (MQ=255)
                                        aaGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTacgacg                                                                                                            >  2:452696/1‑150 (MQ=255)
                                         aGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACgc                                                                                                           >  1:1197501/1‑150 (MQ=255)
                                           cTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGGCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACgcag                                                                                                         <  1:171062/150‑1 (MQ=255)
                                            tGGTGTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACgcagc                                                                                                        <  2:917984/150‑1 (MQ=255)
                                                  ttCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCACCCGCTTACCAg                                                                                                                                         >  1:911966/1‑111 (MQ=255)
                                                  ttCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGGTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATAccc                                                                                                  <  1:1204878/150‑1 (MQ=255)
                                                   tCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCt                                                                                                 >  1:707975/1‑150 (MQ=255)
                                                   tCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCt                                                                                                 >  1:348777/1‑150 (MQ=255)
                                                      ggTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGtt                                                                                              >  2:189959/1‑150 (MQ=255)
                                                      ggTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGtt                                                                                              >  2:1316687/1‑150 (MQ=255)
                                                       gTGAGCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGttt                                                                                             <  2:774674/150‑1 (MQ=255)
                                                         gATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGttttt                                                                                           <  2:399109/150‑1 (MQ=255)
                                                         gATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGttttt                                                                                           <  1:1484807/150‑1 (MQ=255)
                                                          aTCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGACCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGtttttt                                                                                          <  2:136084/150‑1 (MQ=255)
                                                            cGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCa                                                                                        <  2:378649/150‑1 (MQ=255)
                                                             ggTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAg                                                                                       <  1:444124/150‑1 (MQ=255)
                                                             ggTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAg                                                                                       <  2:748665/150‑1 (MQ=255)
                                                                aaGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCca                                                                                    <  2:1201732/150‑1 (MQ=255)
                                                                 accGAAAAACCTGCGACTTTCAGCAGTAAGGAGAGATTCTTGAGCACGGTGATTACCTGTTCTTGAAGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCcac                                                                                   <  2:117681/148‑1 (MQ=255)
                                                                 aGCGAAATACCTGCGTCTTTCACCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTGACCGGGCATTTATAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCcac                                                                                   <  2:210103/150‑1 (MQ=255)
                                                                  gCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTATGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCcaca                                                                                  <  2:92437/150‑1 (MQ=255)
                                                                  gCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCCTACCCTGTTTTTTCAGCcaca                                                                                  >  2:1350261/1‑150 (MQ=255)
                                                                    gAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACGAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACAcc                                                                                <  2:824690/150‑1 (MQ=255)
                                                                     aaaTACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACca                                                                               <  2:1325358/150‑1 (MQ=255)
                                                                       aTACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACcagc                                                                             <  1:60147/150‑1 (MQ=255)
                                                                       aTACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACcagc                                                                             <  2:1291597/150‑1 (MQ=255)
                                                                         aacTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACcagcag                                                                           <  1:626457/148‑1 (MQ=255)
                                                                         aCCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACcagcag                                                                           >  2:474135/1‑150 (MQ=255)
                                                                           cTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGaa                                                                         >  1:891792/1‑150 (MQ=255)
                                                                            caggtatttCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGTGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAAt                                                                        <  1:425366/144‑1 (MQ=255)
                                                                              cGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTAAGCCACACCAGCAGAATgg                                                                      >  1:1384917/1‑150 (MQ=255)
                                                                               gTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCGTACCCGGCATTTTTAATGCGTTATTCGCTACCACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAAAATggt                                                                     >  1:877498/1‑149 (MQ=255)
                                                                                   ttCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATg                                                                 >  2:967959/1‑150 (MQ=255)
                                                                                   ttCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATg                                                                 <  1:1534973/150‑1 (MQ=255)
                                                                                    tCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATCCCCTACGACGCAGCATACCCAGTTTTTTCAGCCACACCAGCAGAATGGAGATgg                                                                >  2:1049525/1‑150 (MQ=255)
                                                                                     cagcagTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCATCCACACCATCAGAATGGAGATGGc                                                               >  1:1056959/1‑150 (MQ=255)
                                                                                       gcagTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCATCATACCCTGTTTTTTAAGCCACACCAGCAGAATGGAGATGGCCg                                                             >  1:1542160/1‑150 (MQ=255)
                                                                                        cagTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGc                                                            >  2:686716/1‑150 (MQ=255)
                                                                                          gTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTCATGCGTTGCCTGGTAAGAGGGTGCTTTCAAGGAATTTTTAATGAGTTATGCGATAATCCGCAGCATACACACTTTAAACAGCAACACCACCAGAATTGAGATGGcctaac                                                          >  1:768092/1‑146 (MQ=255)
                                                                                           tAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAgg                                                         <  1:1379768/150‑1 (MQ=255)
                                                                                            aaGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGc                                                        >  1:255235/1‑150 (MQ=255)
                                                                                            aaGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGc                                                        >  1:1206689/1‑150 (MQ=255)
                                                                                              ggAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGt                                                      >  1:998983/1‑150 (MQ=255)
                                                                                                    tttGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGcc                                                <  2:528405/150‑1 (MQ=255)
                                                                                                     ttgttgAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCa                                               <  1:369079/150‑1 (MQ=255)
                                                                                                         tgAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGaaa                                           >  2:298799/1‑150 (MQ=255)
                                                                                                          gAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAAt                                          <  1:981253/150‑1 (MQ=255)
                                                                                                          gAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAAt                                          >  2:670703/1‑150 (MQ=255)
                                                                                                          gAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAAt                                          >  1:568778/1‑150 (MQ=255)
                                                                                                          gAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCACGCATTTTTAATGCGTTTTGCGCGACGACGCAGCATACCCTGTTTTTTAAGCCACACCAGCAGAATTGAGATGGCCGCAAGCGTGACGCCAGaaaa                                          >  1:1101964/1‑149 (MQ=255)
                                                                                                             cACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTTTGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACCCCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATAcc                                       >  2:1090818/1‑149 (MQ=255)
                                                                                                               cGGTGATTACCTGTTCTTGATGCGTTTCCTGGTAAGCCGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCGACGACGCAGCAAACCCTGTTTTTTCAGCCACACCAGCAGAATGGATAAGGCCGCAGGCGTGACGCCAGAAATAcgcc                                     >  2:1417338/1‑149 (MQ=255)
                                                                                                                      tACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTg                              >  2:573306/1‑150 (MQ=255)
                                                                                                                      tACCTGTTCATGATGCGTTGCCTGGTAAGCGGGGGCTTACCAGGCATTATTAATGCGTTAAGCGCTACGACTCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAAGGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTg                              <  2:853342/150‑1 (MQ=255)
                                                                                                                       aCCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTgg                             >  1:457256/1‑150 (MQ=255)
                                                                                                                         cTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATTCGCTAAGACGCACCATACCCTGTTTTTTAAGCCACACCAGCAGAATGGAGAAGGCCGCCGGCGTGCCGCCAGCAATACGCGAACCTTGGcc                           >  1:1464865/1‑150 (MQ=255)
                                                                                                                          tGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCg                          <  2:148792/150‑1 (MQ=255)
                                                                                                                              cTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGGTACGACGCAGCATACCCTGTTTTTTCAGCCACAGCAGCAGAATGGTGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATag                      >  2:839135/1‑150 (MQ=255)
                                                                                                                              cTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATag                      <  1:381789/150‑1 (MQ=255)
                                                                                                                              cTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACCCGAAGCTTGGCCGATag                      <  1:61485/150‑1 (MQ=255)
                                                                                                                               ttGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATaga                     >  2:1520199/1‑150 (MQ=255)
                                                                                                                                 taTGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAgg                   <  1:1024594/149‑1 (MQ=255)
                                                                                                                                 gATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAgg                   >  2:624809/1‑150 (MQ=255)
                                                                                                                                 gATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAgg                   >  1:1240185/1‑150 (MQ=255)
                                                                                                                                   tGCGTTGCCTTGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGGTATGCGCTACGACGCAGCATACCCTGTTTTATCAGCGACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCt                 <  1:217266/150‑1 (MQ=255)
                                                                                                                                    gctttGCCTGGTAAGCGCGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCCGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTg                <  2:81753/147‑1 (MQ=255)
                                                                                                                                      gTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTTTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGt              <  2:292962/150‑1 (MQ=255)
                                                                                                                                      gTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGt              <  2:1197501/150‑1 (MQ=255)
                                                                                                                                       atGCCTGGTAAGCACCCGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGtt             >  1:1720312/2‑150 (MQ=255)
                                                                                                                                         gCCTGGTAAGCACCCGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTtg           >  2:937307/1‑150 (MQ=255)
                                                                                                                                          ccTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTtgt          <  1:474135/150‑1 (MQ=255)
                                                                                                                                          ccTGGTAAGCACCCGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTtgt          >  2:1164992/1‑150 (MQ=255)
                                                                                                                                              gTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATc      <  1:1299317/150‑1 (MQ=255)
                                                                                                                                               tAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCg     <  2:348777/150‑1 (MQ=255)
                                                                                                                                                aaGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGt    >  1:760210/1‑150 (MQ=255)
                                                                                                                                                aaGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGt    <  1:1656500/150‑1 (MQ=255)
                                                                                                                                                aaGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGt    >  2:1110975/1‑150 (MQ=255)
                                                                                                                                                 aGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGtt   >  2:606631/1‑150 (MQ=255)
                                                                                                                                                  gCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTa  <  1:1053627/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
ACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTA  >  NC_000913/3923569‑3923864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: