Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,923,720 T→C 6.8% intergenic (‑40/+24) rsmG ← / ← mnmG 16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein/5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,923,7200TC 6.8% 416.7 / 16.0 147intergenic (‑40/+24)rsmG/mnmG16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein/5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
Reads supporting (aligned to +/- strand):  ref base T (64/71);  new base C (6/4);  total (70/77)
Fisher's exact test for biased strand distribution p-value = 5.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.10e-01

Only 100 of 149 total aligned reads displayed.

GCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTA  >  NC_000913/3923571‑3923864
                                                                                                                                                     |                                                                                                                                                
gatttGTTCCATTAAAGCAGCATATTCACGTAGGCCATAAGCTGGTGTTTCAGGTGATCGGTAAGCGAAATACATGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGt                                                                                                                                                  <  1:289177/148‑1 (MQ=255)
gCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGt                                                                                                                                                  >  2:1534973/1‑150 (MQ=255)
  tttGTTCCATTTATGCAGCATATTCACGTGGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGGCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGc                                                                                                                                                <  2:326803/150‑1 (MQ=255)
   ttGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCt                                                                                                                                               >  2:1053627/1‑150 (MQ=255)
     gTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTa                                                                                                                                             <  1:1121268/150‑1 (MQ=255)
        ccATTTATGCAGCATATTCACGTAGGCAATAAACTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCa                                                                                                                                          <  1:828445/150‑1 (MQ=255)
         cATTTCTGCAGCATATTCCCGGAGGCAATCAGCTGGGTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCCGTAAGGAGAGGTGGTTGAGCACCGTGATTACCTGGTCTTGATGCGTTGCCTGGTAAGCACCCGCTTACCAg                                                                                                                                         <  1:1060959/150‑1 (MQ=255)
         cATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAg                                                                                                                                         <  1:69615/150‑1 (MQ=255)
         cATTTAAGCAGCATAATCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCACCCGCTTACCAg                                                                                                                                         <  1:1328295/150‑1 (MQ=255)
          aTTTATGCAGCATATTCACGTAGGCAATAAGCGGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGATTGTTGAGCACGGTGATTACCTGTTCTTGATGCGGTGCCTGGTAAGCACCCGCTTACCAgg                                                                                                                                        <  1:903408/150‑1 (MQ=255)
                gcagcaTATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCAtttt                                                                                                                                  >  1:399109/1‑150 (MQ=255)
                gcagcaTATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCAtttt                                                                                                                                  >  2:160660/1‑150 (MQ=255)
                 cagcaTATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCAttttt                                                                                                                                 <  2:1693647/150‑1 (MQ=255)
                      tatTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGc                                                                                                                            >  1:172838/1‑150 (MQ=255)
                       atTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCg                                                                                                                           >  2:628165/1‑150 (MQ=255)
                       atTCACGTAGGCAATAAGCTGGGTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCg                                                                                                                           <  2:1256652/150‑1 (MQ=255)
                        ttCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGt                                                                                                                          <  1:1115866/150‑1 (MQ=255)
                         tCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGtt                                                                                                                         <  2:447188/150‑1 (MQ=255)
                              tAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATtcg                                                                                                                    >  1:1299611/1‑150 (MQ=255)
                              tAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATgcg                                                                                                                    <  1:612659/150‑1 (MQ=255)
                               aGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATgcgc                                                                                                                   <  1:1589944/150‑1 (MQ=255)
                                 gCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCTTTATGCGCTa                                                                                                                 >  1:157973/1‑150 (MQ=255)
                                  cAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTac                                                                                                                <  1:1064514/150‑1 (MQ=255)
                                   aaTAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGATTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCTGGTGCTTACCAGGCATTTTAAATGCCTTATGCGCTacg                                                                                                               >  1:72716/1‑150 (MQ=255)
                                      aaGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTacgacg                                                                                                            >  2:452696/1‑150 (MQ=255)
                                      aaGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTacgacg                                                                                                            >  1:45879/1‑150 (MQ=255)
                                          tGGTGTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACgcagc                                                                                                        <  2:917984/150‑1 (MQ=255)
                                           ggTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACgcagca                                                                                                       <  1:105662/150‑1 (MQ=255)
                                              ttttCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATAc                                                                                                    >  1:826408/1‑150 (MQ=255)
                                                ttCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCACCCGCTTACCAg                                                                                                                                         >  1:911966/1‑111 (MQ=255)
                                                ttCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGGTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATAccc                                                                                                  <  1:1204878/150‑1 (MQ=255)
                                                    ggTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGtt                                                                                              >  2:189959/1‑150 (MQ=255)
                                                    ggTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGtt                                                                                              >  2:1316687/1‑150 (MQ=255)
                                                     gTGAGCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGttt                                                                                             <  2:774674/150‑1 (MQ=255)
                                                       gATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGttttt                                                                                           <  2:399109/150‑1 (MQ=255)
                                                       gATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGttttt                                                                                           <  1:1484807/150‑1 (MQ=255)
                                                        aTCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGACCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGtttttt                                                                                          <  2:136084/150‑1 (MQ=255)
                                                          cGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACTAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCa                                                                                        <  1:1283317/150‑1 (MQ=255)
                                                          cGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCa                                                                                        <  2:378649/150‑1 (MQ=255)
                                                          cGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCAGTTTTTTCa                                                                                        >  1:559863/1‑150 (MQ=255)
                                                           ggTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTTCCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTAAGACGCAGCATACCCTGTTTTTTCAg                                                                                       <  1:453853/150‑1 (MQ=255)
                                                           ggTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAg                                                                                       <  1:444124/150‑1 (MQ=255)
                                                           ggTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAg                                                                                       <  2:748665/150‑1 (MQ=255)
                                                              aaGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCca                                                                                    <  2:1201732/150‑1 (MQ=255)
                                                               accGAAAAACCTGCGACTTTCAGCAGTAAGGAGAGATTCTTGAGCACGGTGATTACCTGTTCTTGAAGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCcac                                                                                   <  2:117681/148‑1 (MQ=255)
                                                               aGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCcac                                                                                   >  1:46714/1‑150 (MQ=255)
                                                               aGCGAAATACCTGCGTCTTTCACCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTGACCGGGCATTTATAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCcac                                                                                   <  2:210103/150‑1 (MQ=255)
                                                                gCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTATGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCcaca                                                                                  <  2:92437/150‑1 (MQ=255)
                                                                gCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCCTACCCTGTTTTTTCAGCcaca                                                                                  >  2:1350261/1‑150 (MQ=255)
                                                                  gAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACGAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACAcc                                                                                <  2:824690/150‑1 (MQ=255)
                                                                   aaaTACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACca                                                                               <  2:1325358/150‑1 (MQ=255)
                                                                     aTACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACcagc                                                                             <  1:1390617/150‑1 (MQ=255)
                                                                     aTACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACcagc                                                                             <  2:1291597/150‑1 (MQ=255)
                                                                            cGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTAAGCCACACCAGCAGAATgg                                                                      >  1:1384917/1‑150 (MQ=255)
                                                                             gTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCGTACCCGGCATTTTTAATGCGTTATTCGCTACCACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAAAATggt                                                                     >  1:877498/1‑149 (MQ=255)
                                                                                 ttCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATg                                                                 >  2:967959/1‑150 (MQ=255)
                                                                                  tCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATCCCCTACGACGCAGCATACCCAGTTTTTTCAGCCACACCAGCAGAATGGAGATgg                                                                >  2:1049525/1‑150 (MQ=255)
                                                                                     gcagTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCATCATACCCTGTTTTTTAAGCCACACCAGCAGAATGGAGATGGCCg                                                             >  1:1542160/1‑150 (MQ=255)
                                                                                      cagTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGc                                                            >  2:686716/1‑150 (MQ=255)
                                                                                          aaGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGc                                                        >  1:255235/1‑150 (MQ=255)
                                                                                            ggAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGt                                                      >  1:998983/1‑150 (MQ=255)
                                                                                            ggAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGt                                                      >  1:831423/1‑150 (MQ=255)
                                                                                                  tttGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGcc                                                <  2:528405/150‑1 (MQ=255)
                                                                                                   ttgttgAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCa                                               <  1:369079/150‑1 (MQ=255)
                                                                                                      ttgAGCACGGTGATTACCTGTTCTTGATGAGTTGCCGGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGaa                                            <  1:1497312/150‑1 (MQ=255)
                                                                                                       tgAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGaaa                                           >  2:298799/1‑150 (MQ=255)
                                                                                                        gAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAAt                                          >  2:670703/1‑150 (MQ=255)
                                                                                                        gAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAAt                                          <  1:981253/150‑1 (MQ=255)
                                                                                                        gAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAAt                                          >  1:568778/1‑150 (MQ=255)
                                                                                                           cACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTTTGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACCCCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATAcc                                       >  2:1090818/1‑149 (MQ=255)
                                                                                                             cGGTGATTACCTGTTCTTGATGCGTTTCCTGGTAAGCCGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCGACGACGCAGCAAACCCTGTTTTTTCAGCCACACCAGCAGAATGGATAAGGCCGCAGGCGTGACGCCAGAAATAcgcc                                     >  2:1417338/1‑149 (MQ=255)
                                                                                                               gTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGaa                                   <  2:511601/150‑1 (MQ=255)
                                                                                                                 taataacTGTTATTGTAGCGGTTCCTGGGAATCGGGGGCATAACATGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGc                                 <  1:1685620/144‑1 (MQ=255)
                                                                                                                    tACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTg                              >  2:573306/1‑150 (MQ=255)
                                                                                                                    tACCTGTTCATGATGCGTTGCCTGGTAAGCGGGGGCTTACCAGGCATTATTAATGCGTTAAGCGCTACGACTCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAAGGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTg                              <  2:853342/150‑1 (MQ=255)
                                                                                                                       cTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGcc                           <  1:371594/150‑1 (MQ=255)
                                                                                                                        tGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCg                          <  2:148792/150‑1 (MQ=255)
                                                                                                                            cTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGGTACGACGCAGCATACCCTGTTTTTTCAGCCACAGCAGCAGAATGGTGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATag                      >  2:839135/1‑150 (MQ=255)
                                                                                                                            cTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACCCGAAGCTTGGCCGATag                      <  1:61485/150‑1 (MQ=255)
                                                                                                                             ttGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATaga                     >  2:1520199/1‑150 (MQ=255)
                                                                                                                              tGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTTCGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAATTACGCGAAGCTTGGCCGATagag                    >  1:68868/1‑150 (MQ=255)
                                                                                                                               taTGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAgg                   <  1:1024594/149‑1 (MQ=255)
                                                                                                                               gATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAgg                   >  2:624809/1‑150 (MQ=255)
                                                                                                                               gATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAgg                   >  1:1240185/1‑150 (MQ=255)
                                                                                                                                aTGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGc                  <  1:1567014/150‑1 (MQ=255)
                                                                                                                                  gctttGCCTGGTAAGCGCGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCCGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTg                <  2:81753/147‑1 (MQ=255)
                                                                                                                                    gTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTTTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGt              <  2:292962/150‑1 (MQ=255)
                                                                                                                                    gTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGt              <  2:1197501/150‑1 (MQ=255)
                                                                                                                                     ttGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGtt             <  1:1205779/150‑1 (MQ=255)
                                                                                                                                     atGCCTGGTAAGCACCCGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGtt             >  1:1720312/2‑150 (MQ=255)
                                                                                                                                       gCCTGGTAAGCACCCGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTtg           >  2:937307/1‑150 (MQ=255)
                                                                                                                                        ccTGGTAAGCACCCGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTtgt          >  2:1164992/1‑150 (MQ=255)
                                                                                                                                         cTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTAAGCCACACCATCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTtgtg         >  1:420356/1‑150 (MQ=255)
                                                                                                                                          gggTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGa        <  1:960296/149‑1 (MQ=255)
                                                                                                                                             tAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCg     <  2:348777/150‑1 (MQ=255)
                                                                                                                                              aaGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGt    <  1:1656500/150‑1 (MQ=255)
                                                                                                                                              aaGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGt    >  2:1110975/1‑150 (MQ=255)
                                                                                                                                              aaGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGt    >  1:760210/1‑150 (MQ=255)
                                                                                                                                               aGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGtt   >  2:606631/1‑150 (MQ=255)
                                                                                                                                                gCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTa  <  1:1053627/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
GCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTA  >  NC_000913/3923571‑3923864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: