Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,937,186 G→A 5.1% intergenic (+18/‑108) rbsB → / → rbsK D‑ribose ABC transporter periplasmic binding protein; ribose chemotaxis receptor/ribokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,937,1860GA 5.1% 380.1 / 5.4 118intergenic (+18/‑108)rbsB/rbsKD‑ribose ABC transporter periplasmic binding protein; ribose chemotaxis receptor/ribokinase
Reads supporting (aligned to +/- strand):  ref base G (59/53);  new base A (3/3);  total (62/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.95e-01

Only 100 of 120 total aligned reads displayed.

CTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGCAAAACGCAGGCAGCCTCGTTGTTCTTGGCAGCATTAAT  >  NC_000913/3937037‑3937335
                                                                                                                                                     |                                                                                                                                                      
atAGCAGCGACTATCGCTCAGCTACCAGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATg                                                                                                                                                        <  1:62682/149‑1 (MQ=255)
   gcagcGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATTAGGTTGTATGAcc                                                                                                                                                     <  1:632178/150‑1 (MQ=255)
   gcagcGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGAcc                                                                                                                                                     <  1:600262/150‑1 (MQ=255)
   gcagcGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGAcc                                                                                                                                                     <  1:1710307/150‑1 (MQ=255)
   gcagcGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGAcc                                                                                                                                                     <  1:1557401/150‑1 (MQ=255)
    aagcGACTATCGCTCTTCTACCCTATCAGATTGGCGAGAAAGTCGTCGAAACCGCAGATAAATTGCTGAAATGAGAGAAAGTTCATGGTAAGTATCCGGTTGATCTGAATCTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCt                                                                                                                                                    <  1:236298/149‑1 (MQ=255)
     agcGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTg                                                                                                                                                   <  2:177966/150‑1 (MQ=255)
      gcGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGa                                                                                                                                                  >  1:758128/1‑150 (MQ=255)
        gACTATCGCTCAGCTACCCGATCAGAATGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTCATACAACCTGAtt                                                                                                                                                <  1:87923/150‑2 (MQ=255)
         aCTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTCATACAACCTGAtta                                                                                                                                               <  2:709322/150‑3 (MQ=255)
          cTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGGCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGt                                                                                                                                              <  2:1286083/150‑1 (MQ=255)
           tATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTg                                                                                                                                             <  2:408899/150‑1 (MQ=255)
           tATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTg                                                                                                                                             >  1:912825/1‑150 (MQ=255)
           tATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTg                                                                                                                                             <  2:168437/150‑1 (MQ=255)
           tATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTg                                                                                                                                             <  1:1261212/150‑1 (MQ=255)
            aTCGCTCAGCTACGCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGa                                                                                                                                            <  1:625209/150‑1 (MQ=255)
            aTCGCACAGGTACCCGAGCAGATTTGCGCGAAAGGCGGCGAAACCGCAGTTAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGTTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGa                                                                                                                                            <  2:820191/150‑1 (MQ=255)
             tCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTCATACAACCTGAttaaaac                                                                                                                                           <  2:1208932/150‑7 (MQ=255)
              cGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACa                                                                                                                                          >  1:30740/1‑150 (MQ=255)
              cGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACa                                                                                                                                          <  1:794416/150‑1 (MQ=255)
              cGCTCAGCTAACCGATCAGATTGGCGCGACAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACa                                                                                                                                          <  1:241159/150‑1 (MQ=255)
               gcgcAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGATGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACAt                                                                                                                                         <  1:1334008/147‑1 (MQ=255)
               gCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACAt                                                                                                                                         <  1:1444032/150‑1 (MQ=255)
               gCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTATAATCAGGTTGTATGACCTGATGGTGACAt                                                                                                                                         <  1:1562823/150‑1 (MQ=255)
                cTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATa                                                                                                                                        >  1:853454/1‑150 (MQ=255)
                   aGCTACCCGATCTGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAAt                                                                                                                                     <  1:1366259/150‑1 (MQ=255)
                   aGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAAt                                                                                                                                     >  1:1326783/1‑150 (MQ=255)
                   aGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAAt                                                                                                                                     >  1:671150/1‑150 (MQ=255)
                   aGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAAt                                                                                                                                     >  2:1594780/1‑150 (MQ=255)
                     cTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGAAAAAGTGCTGAAAGGCGAGAAAGTTCAGCCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGAAGCTGACATAAATAc                                                                                                                                   >  2:841990/1‑150 (MQ=255)
                              cAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGAc                                                                                                                          >  2:1332945/1‑150 (MQ=255)
                                gATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAg                                                                                                                        >  1:1725411/1‑150 (MQ=255)
                                  ttGGCGCGAAAGGCGTCGAAACGGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGAt                                                                                                                      >  2:1000116/1‑150 (MQ=255)
                                   tGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATg                                                                                                                     <  1:1150342/150‑1 (MQ=255)
                                     gcgcGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGaa                                                                                                                   >  1:1195060/1‑150 (MQ=255)
                                      cgcgAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAAc                                                                                                                  >  1:1113162/1‑150 (MQ=255)
                                         gAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACgtg                                                                                                               <  2:1374515/150‑1 (MQ=255)
                                           aaGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTa                                                                                                             <  2:798205/150‑1 (MQ=255)
                                           aaGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATAAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTa                                                                                                             <  2:929080/150‑1 (MQ=255)
                                            aGGCGTCGAAACCGCAGATAAAGGGCTGAAAGGCGAGAAAGTTCAGGCTATGAATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTGGTATGACCTGATGGTGACATAAATACGGCATCGACAGATGAACGTGTaa                                                                                                            <  2:976167/150‑1 (MQ=255)
                                             ggCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCTGTTGATCTGAAACTGGGTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATCCGTCATCGACAGATGAACGTGTAat                                                                                                           <  1:612864/150‑1 (MQ=255)
                                                gTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAAtata                                                                                                        <  1:769836/150‑1 (MQ=255)
                                                   gAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAg                                                                                                     <  1:1469122/150‑1 (MQ=255)
                                                         gCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGc                                                                                               >  1:1691005/1‑150 (MQ=255)
                                                            gATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAgg                                                                                           >  1:1432858/1‑150 (MQ=255)
                                                             aTAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAggg                                                                                          <  2:470016/150‑1 (MQ=255)
                                                              tAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGc                                                                                         >  1:1259063/1‑150 (MQ=255)
                                                                aaGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCAc                                                                                       >  2:839306/1‑150 (MQ=255)
                                                                 aGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCAcg                                                                                      >  1:571584/1‑150 (MQ=255)
                                                                  gTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCAcgc                                                                                     >  1:1050009/1‑150 (MQ=255)
                                                                    gCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTATTCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACgcgc                                                                                   >  2:1417076/1‑150 (MQ=255)
                                                                       gAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCAc                                                                                <  2:1279120/150‑1 (MQ=255)
                                                                       gAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCAc                                                                                >  1:1285430/1‑150 (MQ=255)
                                                                        aaaGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTATTCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCAcc                                                                               <  1:1417076/150‑1 (MQ=255)
                                                                        aaaGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCAcc                                                                               <  2:536923/150‑1 (MQ=255)
                                                                        aaaGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCAcc                                                                               <  1:1673928/150‑1 (MQ=255)
                                                                                gaAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGAAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACg                                                                       >  2:921788/1‑150 (MQ=255)
                                                                                    gTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGTGCACGCGCCACCCTAACACGGTgg                                                                   >  1:692853/1‑150 (MQ=255)
                                                                                     ttcttgCTAAGTTTGCTGTTGATCTGAATCTGGTTGTTAAGCTGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGGCACGCGCCACCCTAACACGGTgg                                                                   <  2:1144848/145‑1 (MQ=255)
                                                                                      tCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGgcg                                                                 >  1:1018437/1‑150 (MQ=255)
                                                                                      tCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGgcg                                                                 <  2:526703/150‑1 (MQ=255)
                                                                                      tCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGgcg                                                                 >  2:70657/1‑150 (MQ=255)
                                                                                       cAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGgcgc                                                                <  2:883261/150‑1 (MQ=255)
                                                                                         ggCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCAt                                                              <  1:814639/150‑1 (MQ=255)
                                                                                         ggCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCAt                                                              >  1:143945/1‑150 (MQ=255)
                                                                                         ggCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCAt                                                              >  1:1560922/1‑150 (MQ=255)
                                                                                           cTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCAttt                                                            <  2:1499847/150‑1 (MQ=255)
                                                                                              aGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTAt                                                         >  1:427455/1‑150 (MQ=255)
                                                                                                tATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATgg                                                       <  2:1626859/150‑1 (MQ=255)
                                                                                                 aTCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGa                                                      >  1:166713/1‑150 (MQ=255)
                                                                                                  tCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGAc                                                     <  2:853454/150‑1 (MQ=255)
                                                                                                    cGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACAt                                                   >  2:1339595/1‑150 (MQ=255)
                                                                                                      gTTGATCTGAAACTGGTTGTTAAGCAGAAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGGCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATcc                                                 <  2:1681466/150‑1 (MQ=255)
                                                                                                            cTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCTCGCGCCCCCCTAACACGGTGGCGCATTTTATGGACAGCCCGAata                                           >  2:1055823/1‑150 (MQ=255)
                                                                                                             tGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGTCACGCGCCACCCTAACACGGTGGCGCATGTTATGGACATACCGAataa                                          >  2:692389/1‑149 (MQ=255)
                                                                                                             tGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAatat                                          >  2:644383/1‑150 (MQ=255)
                                                                                                             tGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAatat                                          >  1:687118/1‑150 (MQ=255)
                                                                                                              gAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATg                                         >  1:521759/1‑150 (MQ=255)
                                                                                                               aaaCTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGc                                        <  2:1252404/150‑1 (MQ=255)
                                                                                                               aaaCTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGc                                        >  1:283390/1‑150 (MQ=255)
                                                                                                                 aCTGGTTGTTAAGCAGTAGTTTTAATCAGTTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCTGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGATTATGCaa                                      <  1:1508019/150‑1 (MQ=255)
                                                                                                                    ggTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGCAAAAc                                   <  2:30740/150‑1 (MQ=255)
                                                                                                                     gttgttAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGCAAAACg                                  <  2:1725411/150‑1 (MQ=255)
                                                                                                                          tAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGCAAAACgcaggc                             >  1:86990/1‑150 (MQ=255)
                                                                                                                            aGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGCAAAACgcaggcag                           >  2:163219/1‑150 (MQ=255)
                                                                                                                            aGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGCAAAACgcaggcag                           >  2:1446696/1‑150 (MQ=255)
                                                                                                                               agtagtTTTAATCAGGTGGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGCAAAACGCAGGCAGCCt                        <  2:163058/150‑1 (MQ=255)
                                                                                                                                  agtTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGCAAAACGCAGGCAGCCTCgg                     >  1:1568933/1‑149 (MQ=255)
                                                                                                                                   gtcaccaTCAGGTCATACAACCTGATGGTGCCATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCCCCCTAACACGGTGGCGCATTTTATGGACATCCAGAAAATGCAAAACGCAGGCAGACTCgtt                    >  1:965293/7‑150 (MQ=255)
                                                                                                                                    ttttAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGCAAAACGCAGGCAGCCTCgttg                   <  2:912825/150‑1 (MQ=255)
                                                                                                                                    ttttAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCACATTTTATGGACATCCCGAATATGCAAAACGCAGGCAGCCTCgttg                   >  1:257367/1‑150 (MQ=255)
                                                                                                                                       tAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGCAAAACGCTGGCCGCCTCgttgtta                >  1:1398855/1‑149 (MQ=255)
                                                                                                                                       tAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGCAAAACGCAGGCAGCCTCGTTGTTc                >  2:739884/1‑150 (MQ=255)
                                                                                                                                       ccaTCAGGTCATACAACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGCAAAACGCAGGCAGCCTCGTTGTTc                >  1:971347/3‑150 (MQ=255)
                                                                                                                                         aTCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACAAGGTGGCGCATTTTATGGACATCCCTAATATGCAAAAAGCATGCAGCCTAGTTGTTCtt              >  1:578827/1‑150 (MQ=255)
                                                                                                                                            aGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGCTGGCGCATTTTATGGACATCCCGAATATGCAAAACGCAGGAAGCATCGTTGTTCTTGgc           >  1:103907/1‑150 (MQ=255)
                                                                                                                                             ggTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGCAAAACGCAGGCAGCCTCGTTGTTCTTGgca          >  1:1051294/1‑150 (MQ=255)
                                                                                                                                              gTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGCAAACCGCAGGCAGCCTCGTTGTTCTTGgcag         >  2:260697/1‑150 (MQ=255)
                                                                                                                                              gTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGCAAAACGCAGGCAGCCTCGTTGTTCTTGgcag         <  1:986573/150‑1 (MQ=255)
                                                                                                                                                     gACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGCAAAACGCAGGCAGCCTCGTTGTTCTTGGCAGCATTAAt  >  1:1626823/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGCAAAACGCAGGCAGCCTCGTTGTTCTTGGCAGCATTAAT  >  NC_000913/3937037‑3937335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: