Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,947,332 A→T 5.8% A212A (GCT→GCA hdfR ← flhDC operon transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,947,3320AT 5.8% 324.9 / 6.0 113A212A (GCT→GCAhdfRflhDC operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (57/48);  new base T (3/4);  total (60/53)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

TATCGCTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTGAAAT  >  NC_000913/3947184‑3947480
                                                                                                                                                     |                                                                                                                                                    
tATCGCTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTcag                                                                                                                                                     >  1:1659063/1‑150 (MQ=255)
  tCGCTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTTAGCATCGCAATCTGTTGCTGTGCCAGTTcagcc                                                                                                                                                   >  1:1414213/1‑149 (MQ=255)
  tCGCTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTcagca                                                                                                                                                   >  2:945870/1‑150 (MQ=255)
  acGCTATTTTTCAGCCATATGGCATAAAGCGGCCGTGAAAGTTTTGTGCTATCGACAACGGTATGCAGGCCGCATTTTTTACGCGCCCATCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTTCTGTGCCAGATcagca                                                                                                                                                   <  1:153498/149‑1 (MQ=255)
   cGCTATTTTGCCGCCATATGGCATAAAGCGGCCGTGAAAGTGTGGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTcagcag                                                                                                                                                  <  2:1535701/150‑1 (MQ=255)
   cGCTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCCGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTcagcag                                                                                                                                                  >  1:286356/1‑150 (MQ=255)
    gCTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAGTCTGTTGCTGTGCCAGTTCAGCAGa                                                                                                                                                 >  2:956946/1‑150 (MQ=255)
      tATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGGTATCGACAACGGTATGCAGGCCGCCTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACt                                                                                                                                              <  2:1283974/150‑1 (MQ=255)
      tATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAAc                                                                                                                                               <  2:497833/150‑1 (MQ=255)
      tATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAAc                                                                                                                                               <  2:1064036/150‑1 (MQ=255)
       aTTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACt                                                                                                                                              <  1:1240103/150‑1 (MQ=255)
       aTTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGGGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACt                                                                                                                                              <  2:494905/150‑1 (MQ=255)
         tttGCAGCCATCTGGCATAAACCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTgg                                                                                                                                            <  2:327958/150‑1 (MQ=255)
          ttGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCTGCTGAACTggc                                                                                                                                           <  1:446086/150‑2 (MQ=255)
          ttGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGt                                                                                                                                           <  2:226550/150‑1 (MQ=255)
            gCAGCCATATGGCATAAAGCGGCCGTGAATGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCTGCTGAACTggcac                                                                                                                                         <  1:1265139/150‑4 (MQ=255)
            gCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTg                                                                                                                                         <  2:691427/150‑1 (MQ=255)
            gCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTg                                                                                                                                         <  2:1509896/150‑1 (MQ=255)
             cAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGt                                                                                                                                        >  1:855780/1‑150 (MQ=255)
             cAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGAAAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAACTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGt                                                                                                                                        >  1:395514/1‑150 (MQ=255)
               gCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCa                                                                                                                                      >  2:1371913/1‑150 (MQ=255)
               gCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCa                                                                                                                                      >  1:925980/1‑150 (MQ=255)
                ccATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAg                                                                                                                                     <  1:1577148/150‑1 (MQ=255)
                 cATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTATTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGCTCTGGATGTCAGa                                                                                                                                    >  1:271568/1‑150 (MQ=255)
                       gCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGGCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGc                                                                                                                              >  1:1358794/1‑150 (MQ=255)
                       gCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGc                                                                                                                              <  1:586057/150‑1 (MQ=255)
                       gCATAAAGCGGCCGTGAAAGGGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTTTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGc                                                                                                                              <  2:272274/150‑1 (MQ=255)
                             aGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGAGCAACCATTAAGCATCGCAATCTGTTGCAGTGCCAGTTCACCAGAACAGCTTGTCACAATGGGCACTTCg                                                                                                                        >  2:910980/1‑150 (MQ=255)
                               cGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTc                                                                                                                      <  2:904459/150‑1 (MQ=255)
                                 gCCGTGACCGTGGTCTGCTAACGACAACGGTATGCAGGCCGCCTTTTTTACGCGCGCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGACAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAg                                                                                                                    <  1:331754/150‑1 (MQ=255)
                                   cGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCa                                                                                                                  <  2:668265/150‑1 (MQ=255)
                                    gTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCAc                                                                                                                 >  2:1279573/1‑150 (MQ=255)
                                     tGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCAcc                                                                                                                <  1:753789/150‑1 (MQ=255)
                                      gAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCg                                                                                                               >  1:1336004/1‑150 (MQ=255)
                                          gtgtTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCa                                                                                                           >  2:1236141/1‑150 (MQ=255)
                                             ttgtGCTCTCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAAc                                                                                                        <  1:919449/150‑1 (MQ=255)
                                               gtgCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAACTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTGGTCAGAATGGGCACTTCGTAAGCACCGATCAAACCt                                                                                                      >  2:938478/1‑150 (MQ=255)
                                                 gCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGc                                                                                                    >  1:1614425/1‑150 (MQ=255)
                                                    aTCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTc                                                                                                 >  2:461505/1‑150 (MQ=255)
                                                      cGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCAt                                                                                               >  2:512811/1‑150 (MQ=255)
                                                        aCAATGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGc                                                                                             <  2:1427613/150‑1 (MQ=255)
                                                        aCAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGc                                                                                             <  1:408014/150‑1 (MQ=255)
                                                              gTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCAGTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGGTGTCAGAATGGGCACTGCGTCCGCACCGCTCAAACCTGCCTCATGCTGTTGa                                                                                       >  2:172735/1‑150 (MQ=255)
                                                               tATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGa                                                                                       >  2:1589029/1‑150 (MQ=255)
                                                                 tGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGaaaa                                                                                    >  2:568593/1‑150 (MQ=255)
                                                                     ggCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTg                                                                                >  1:380901/1‑150 (MQ=255)
                                                                     ggCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTg                                                                                <  2:57828/150‑1 (MQ=255)
                                                                       ccgccTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGc                                                                              >  1:816099/1‑150 (MQ=255)
                                                                        cgccTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGCTCAAACCTGCCTCCTGCTGTTGAAAATCTGGcc                                                                             >  1:1119115/1‑150 (MQ=255)
                                                                        cgccTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGcc                                                                             <  2:925980/150‑1 (MQ=255)
                                                                         gccTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGccc                                                                            >  2:861103/1‑150 (MQ=255)
                                                                           cTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGccccc                                                                          >  1:1320469/1‑150 (MQ=255)
                                                                               tttACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTc                                                                      >  2:567222/1‑150 (MQ=255)
                                                                                  aCGCGCCCAGCTGACGGGTAGCAAGGTGCAACAATTAAGCATCGCAAACTATTGCTGTGCCAGTTAAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACACAAg                                                                   >  2:1674220/1‑150 (MQ=255)
                                                                                    gcgcCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGTACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTc                                                                 <  1:128514/150‑1 (MQ=255)
                                                                                    gcgcCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCCCTCAAGTc                                                                 >  1:552669/1‑150 (MQ=255)
                                                                                        ccAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATTGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAg                                                             <  1:422042/150‑1 (MQ=255)
                                                                                         cAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGa                                                            >  1:908181/1‑150 (MQ=255)
                                                                                            cTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAACCCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATaa                                                         >  1:918825/1‑150 (MQ=255)
                                                                                             ggACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAAt                                                        <  1:1351251/149‑1 (MQ=255)
                                                                                                    gTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGAt                                                  >  1:784386/1‑150 (MQ=255)
                                                                                                     tAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGAtc                                                 <  2:1410593/150‑1 (MQ=255)
                                                                                                     tAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGAtc                                                 <  1:751012/150‑1 (MQ=255)
                                                                                                      aGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGAtct                                                >  2:402022/1‑150 (MQ=255)
                                                                                                        ccAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTcc                                              <  1:440128/150‑1 (MQ=255)
                                                                                                           ggTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCCCTCAAGTCGCAGATAATTATGATCTCCCtt                                           >  1:1625448/1‑150 (MQ=255)
                                                                                                            gTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCttt                                          >  1:664099/1‑150 (MQ=255)
                                                                                                               cAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAACCCTGCCTCATGCTGTTGAAAATCTGGCACCCACTCAAGTCGCAGCTAATTAAGATCTCACTTTAGt                                       >  1:1093743/1‑150 (MQ=255)
                                                                                                               cAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGt                                       >  2:767992/1‑150 (MQ=255)
                                                                                                                   cATTAAGCATCGCTATCTGTTGCTGTTCTAGTTCAGCATAACTGGTTTTAAGAATGGGCACTTCGTCAGCACCGATCAAACCTTCCTCATGCTGTTGAAAATCTGGCCCCGACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTg                                   <  2:1146140/150‑1 (MQ=255)
                                                                                                                       aaGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAgg                               >  2:1327859/1‑150 (MQ=255)
                                                                                                                        cgCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGACGTGGTTGTCAGAATTGGCACTTCGTCAGCACCTATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGCCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAggg                              <  2:147104/149‑1 (MQ=255)
                                                                                                                        aGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCATCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAggg                              <  1:245826/150‑1 (MQ=255)
                                                                                                                        aGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAggg                              >  2:1147394/1‑150 (MQ=255)
                                                                                                                        aGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCAACGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAggg                              <  2:513170/150‑1 (MQ=255)
                                                                                                                         gCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAgggg                             <  1:1432444/150‑1 (MQ=255)
                                                                                                                         gCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAgggg                             <  2:1501569/150‑1 (MQ=255)
                                                                                                                          cATCGCAATCTGTTGCTTTGCCAGTTCAGCAGAACGGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGTTCTCCCTTTAGTTTTGAAGGGGc                            <  1:1285086/150‑1 (MQ=255)
                                                                                                                          cATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGc                            >  2:992744/1‑150 (MQ=255)
                                                                                                                           aTCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGGTTTGAAGGGGcc                           >  2:1708898/1‑149 (MQ=255)
                                                                                                                             cGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACt                         >  2:710805/1‑150 (MQ=255)
                                                                                                                             cGCAATCTGTTGCTGTGCCAGTGCAGCAGAACTGGTTGGCTGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACt                         <  2:1195270/150‑1 (MQ=255)
                                                                                                                                  tCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGtat                    <  2:1245186/150‑1 (MQ=255)
                                                                                                                                    ggTTGGTGTGCCAGTGCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCCGCACCGATCAAACCTGCCTAATGCTGTTGAAAATCTGGCCCCCACTCAATTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATaa                  <  2:286356/149‑1 (MQ=255)
                                                                                                                                     gTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCACTTTAGTTTTGAAGGGGCACTGGTATaaa                 >  1:1241819/1‑150 (MQ=255)
                                                                                                                                      ttGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAg                >  1:1703831/1‑150 (MQ=255)
                                                                                                                                      ttGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAg                <  1:1407413/150‑1 (MQ=255)
                                                                                                                                       tGCTGTGCCAGTTCAGCAGATCTGGTTGTCAGAATGGGCACTTCGTCAGCTCCGATCAAGCCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGAGAAGTACGATCTCCCTTTTGTTTTGAAGGGGCACTGGTATAAAgc               >  1:215976/1‑150 (MQ=255)
                                                                                                                                         gacaaccAGTTCTGCTGAACTGGTTGTCAGAATGGGCACTTCGTCAGCCCCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCCCTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAgcgc             >  2:264123/6‑150 (MQ=255)
                                                                                                                                         cTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAgcgc             <  2:1267316/150‑1 (MQ=255)
                                                                                                                                           caaccAGTTCTGCTGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCATCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAACATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTa           >  1:667131/4‑150 (MQ=255)
                                                                                                                                            tgCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCGTTTAGTTTTGATGGGGCACTGGTATAAAGCGCTaa          >  1:1149292/1‑150 (MQ=255)
                                                                                                                                            aaccAGTTCTGCTGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTaa          >  1:444617/3‑150 (MQ=255)
                                                                                                                                              ccAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAg        <  2:44007/150‑1 (MQ=255)
                                                                                                                                               cAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGt       >  1:1470919/1‑150 (MQ=255)
                                                                                                                                                aGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTATAGTTTTGAAGGGGCACTGGAATAAAGCGCTAAAGTg      >  1:46040/1‑150 (MQ=255)
                                                                                                                                                 gTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTGa     >  1:440269/1‑150 (MQ=255)
                                                                                                                                                 gTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTGa     >  1:1517257/1‑150 (MQ=255)
                                                                                                                                                  ttCCGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTGaa    <  1:956946/150‑1 (MQ=255)
                                                                                                                                                    cagcagAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCCCTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTGAAat  >  2:931261/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TATCGCTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTGAAAT  >  NC_000913/3947184‑3947480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: