Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,947,335 A→T 5.1% S211S (TCT→TCA hdfR ← flhDC operon transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,947,3350AT 5.1% 338.0 / 3.8 113S211S (TCT→TCAhdfRflhDC operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (57/50);  new base T (3/3);  total (60/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

TCGCTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTGAAATATC  >  NC_000913/3947186‑3947483
                                                                                                                                                      |                                                                                                                                                    
tCGCTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTcagca                                                                                                                                                      >  2:945870/1‑150 (MQ=255)
acGCTATTTTTCAGCCATATGGCATAAAGCGGCCGTGAAAGTTTTGTGCTATCGACAACGGTATGCAGGCCGCATTTTTTACGCGCCCATCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTTCTGTGCCAGATcagca                                                                                                                                                      <  1:153498/149‑1 (MQ=255)
 cGCTATTTTGCCGCCATATGGCATAAAGCGGCCGTGAAAGTGTGGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTcagcag                                                                                                                                                     <  2:1535701/150‑1 (MQ=255)
 cGCTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCCGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTcagcag                                                                                                                                                     >  1:286356/1‑150 (MQ=255)
  gCTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAGTCTGTTGCTGTGCCAGTTCAGCAGa                                                                                                                                                    >  2:956946/1‑150 (MQ=255)
    tATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGGTATCGACAACGGTATGCAGGCCGCCTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACt                                                                                                                                                 <  2:1283974/150‑1 (MQ=255)
    tATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAAc                                                                                                                                                  <  2:497833/150‑1 (MQ=255)
    tATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAAc                                                                                                                                                  <  2:1064036/150‑1 (MQ=255)
     aTTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACt                                                                                                                                                 <  1:1240103/150‑1 (MQ=255)
       tttGCAGCCATCTGGCATAAACCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTgg                                                                                                                                               <  2:327958/150‑1 (MQ=255)
        ttGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGt                                                                                                                                              <  2:226550/150‑1 (MQ=255)
          gCAGCCATATGGCATAAAGCGGCCGTGAATGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCTGCTGAACTggcac                                                                                                                                            <  1:1265139/150‑4 (MQ=255)
          gCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTg                                                                                                                                            <  2:691427/150‑1 (MQ=255)
          gCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTg                                                                                                                                            <  2:1509896/150‑1 (MQ=255)
           cAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCTGCTGAACTggcaca                                                                                                                                           <  1:1602739/150‑5 (MQ=255)
           cAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGt                                                                                                                                           <  2:1445875/150‑1 (MQ=255)
           cAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGAAAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAACTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGt                                                                                                                                           >  1:395514/1‑150 (MQ=255)
             gCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCa                                                                                                                                         >  2:1371913/1‑150 (MQ=255)
             gCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCa                                                                                                                                         >  1:925980/1‑150 (MQ=255)
              ccATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAg                                                                                                                                        <  1:1577148/150‑1 (MQ=255)
                 tatGTCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAAt                                                                                                                                     <  1:178573/150‑1 (MQ=255)
                     gCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGGCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGc                                                                                                                                 >  1:1358794/1‑150 (MQ=255)
                     gCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGc                                                                                                                                 <  1:586057/150‑1 (MQ=255)
                     gCATAAAGCGGCCGTGAAAGGGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTTTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGc                                                                                                                                 <  2:272274/150‑1 (MQ=255)
                         aaaGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACtt                                                                                                                             >  1:690028/1‑150 (MQ=255)
                           aGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGAGCAACCATTAAGCATCGCAATCTGTTGCAGTGCCAGTTCACCAGAACAGCTTGTCACAATGGGCACTTCg                                                                                                                           >  2:910980/1‑150 (MQ=255)
                             cGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTc                                                                                                                         <  2:904459/150‑1 (MQ=255)
                             cGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTc                                                                                                                         >  1:513170/1‑150 (MQ=255)
                               gCCGTGACCGTGGTCTGCTAACGACAACGGTATGCAGGCCGCCTTTTTTACGCGCGCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGACAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAg                                                                                                                       <  1:331754/150‑1 (MQ=255)
                                 cGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCACCCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCa                                                                                                                     >  2:831895/1‑150 (MQ=255)
                                 cGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCa                                                                                                                     <  2:668265/150‑1 (MQ=255)
                                  gTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCAc                                                                                                                    >  2:1279573/1‑150 (MQ=255)
                                   tGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCAcc                                                                                                                   <  1:753789/150‑1 (MQ=255)
                                    gAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCg                                                                                                                  >  2:245826/1‑150 (MQ=255)
                                           ttgtGCTCTCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAAc                                                                                                           <  1:919449/150‑1 (MQ=255)
                                             gtgCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAACTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTGGTCAGAATGGGCACTTCGTAAGCACCGATCAAACCt                                                                                                         >  2:938478/1‑150 (MQ=255)
                                               gCTATCGACAACGGGATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGAAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGc                                                                                                       <  1:876012/150‑1 (MQ=255)
                                                cTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTGCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGcc                                                                                                      >  1:373720/1‑150 (MQ=255)
                                                  aTCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTc                                                                                                    >  2:461505/1‑150 (MQ=255)
                                                    cGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCAt                                                                                                  >  2:512811/1‑150 (MQ=255)
                                                     gACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCCACCATTAAGCATCGCAATCTGTTGCTGTCACAGTTCAGCAGAACTGCTTGTCAGAATGGGCACTTCGTCAGCCCCGATCAACCCTGCCTCATg                                                                                                 >  2:668098/1‑150 (MQ=255)
                                                      aCAATGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGc                                                                                                <  2:1427613/150‑1 (MQ=255)
                                                            gTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCAGTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGGTGTCAGAATGGGCACTGCGTCCGCACCGCTCAAACCTGCCTCATGCTGTTGa                                                                                          >  2:172735/1‑150 (MQ=255)
                                                             tATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGa                                                                                          >  2:1589029/1‑150 (MQ=255)
                                                                  aGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCt                                                                                    <  2:1320282/150‑1 (MQ=255)
                                                                   ggCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTg                                                                                   >  1:380901/1‑150 (MQ=255)
                                                                   ggCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTg                                                                                   <  2:57828/150‑1 (MQ=255)
                                                                     ccgccTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGc                                                                                 >  1:816099/1‑150 (MQ=255)
                                                                      cgccTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGCTCAAACCTGCCTCCTGCTGTTGAAAATCTGGcc                                                                                >  1:1119115/1‑150 (MQ=255)
                                                                      cgccTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGcc                                                                                <  2:925980/150‑1 (MQ=255)
                                                                       gccTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGccc                                                                               >  2:861103/1‑150 (MQ=255)
                                                                         cTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGccccc                                                                             >  1:1320469/1‑150 (MQ=255)
                                                                         cTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGccccc                                                                             <  2:1615950/150‑1 (MQ=255)
                                                                             tttACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTc                                                                         >  2:567222/1‑150 (MQ=255)
                                                                                aCGCGCCCAGCTGACGGGTAGCAAGGTGCAACAATTAAGCATCGCAAACTATTGCTGTGCCAGTTAAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACACAAg                                                                      >  2:1674220/1‑150 (MQ=255)
                                                                                  gcgcCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGTACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTc                                                                    <  1:128514/150‑1 (MQ=255)
                                                                                  gcgcCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCCCTCAAGTc                                                                    >  1:552669/1‑150 (MQ=255)
                                                                                      ccAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATTGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAg                                                                <  1:422042/150‑1 (MQ=255)
                                                                                       cAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCACGTCGCAGa                                                               >  1:436310/1‑150 (MQ=255)
                                                                                       cAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGa                                                               >  1:908181/1‑150 (MQ=255)
                                                                                          cTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAACCCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATaa                                                            >  1:918825/1‑150 (MQ=255)
                                                                                           ggACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAAt                                                           <  1:1351251/149‑1 (MQ=255)
                                                                                                  gTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGAt                                                     >  1:784386/1‑150 (MQ=255)
                                                                                                   tAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGAtc                                                    <  2:1410593/150‑1 (MQ=255)
                                                                                                   tAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGAtc                                                    <  1:751012/150‑1 (MQ=255)
                                                                                                    aGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGAtct                                                   >  2:402022/1‑150 (MQ=255)
                                                                                                      ccAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTcc                                                 <  1:440128/150‑1 (MQ=255)
                                                                                                         ggTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCCCTCAAGTCGCAGATAATTATGATCTCCCtt                                              >  1:1625448/1‑150 (MQ=255)
                                                                                                          gTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCttt                                             >  1:664099/1‑150 (MQ=255)
                                                                                                            gCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCTGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAg                                           >  2:505073/1‑150 (MQ=255)
                                                                                                             cAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAACCCTGCCTCATGCTGTTGAAAATCTGGCACCCACTCAAGTCGCAGCTAATTAAGATCTCACTTTAGt                                          >  1:1093743/1‑150 (MQ=255)
                                                                                                                ccATTAAGCATCGCAATCTGTGGCTGTGCCAGTTCTGGAGAACTGGTTGTCAGTATGGGCACTTCGTCAGCACGGATCAAACCTGCCTCATGCTGTTGAAATTCTGGCCCCGACTGAAGTCGCAGATAATTAAGATCTCCCTTTAGtttt                                       <  1:1258491/150‑1 (MQ=255)
                                                                                                                 cATTAAGCATCGCTATCTGTTGCTGTTCTAGTTCAGCATAACTGGTTTTAAGAATGGGCACTTCGTCAGCACCGATCAAACCTTCCTCATGCTGTTGAAAATCTGGCCCCGACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTg                                      <  2:1146140/150‑1 (MQ=255)
                                                                                                                      cgCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGACGTGGTTGTCAGAATTGGCACTTCGTCAGCACCTATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGCCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAggg                                 <  2:147104/149‑1 (MQ=255)
                                                                                                                      aGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCATCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAggg                                 <  1:245826/150‑1 (MQ=255)
                                                                                                                      aGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAggg                                 >  2:1147394/1‑150 (MQ=255)
                                                                                                                      aGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCAACGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAggg                                 <  2:513170/150‑1 (MQ=255)
                                                                                                                        cATCGCAATCTGTTGCTTTGCCAGTTCAGCAGAACGGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGTTCTCCCTTTAGTTTTGAAGGGGc                               <  1:1285086/150‑1 (MQ=255)
                                                                                                                        cATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGc                               >  2:992744/1‑150 (MQ=255)
                                                                                                                           cGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACt                            >  2:710805/1‑150 (MQ=255)
                                                                                                                           cGCAATCTGTTGCTGTGCCAGTGCAGCAGAACTGGTTGGCTGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACt                            <  2:1195270/150‑1 (MQ=255)
                                                                                                                                tCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGtat                       <  2:1245186/150‑1 (MQ=255)
                                                                                                                                    ttGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAg                   <  1:1407413/150‑1 (MQ=255)
                                                                                                                                    ttGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAg                   >  1:1703831/1‑150 (MQ=255)
                                                                                                                                     tGCTGTGCCAGTTCAGCAGATCTGGTTGTCAGAATGGGCACTTCGTCAGCTCCGATCAAGCCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGAGAAGTACGATCTCCCTTTTGTTTTGAAGGGGCACTGGTATAAAgc                  >  1:215976/1‑150 (MQ=255)
                                                                                                                                      gCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAgcg                 >  1:1337782/1‑150 (MQ=255)
                                                                                                                                       gacaaccAGTTCTGCTGAACTGGTTGTCAGAATGGGCACTTCGTCAGCCCCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCCCTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAgcgc                >  2:264123/6‑150 (MQ=255)
                                                                                                                                       cTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAgcgc                <  2:427534/150‑1 (MQ=255)
                                                                                                                                       cTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAgcgc                <  2:1267316/150‑1 (MQ=255)
                                                                                                                                          tgCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCGTTTAGTTTTGATGGGGCACTGGTATAAAGCGCTaa             >  1:1149292/1‑150 (MQ=255)
                                                                                                                                          aaccAGTTCTGCTGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTaa             >  1:444617/3‑150 (MQ=255)
                                                                                                                                           gCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAAACTGCCTCATGCTGATGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCATAAGTTTTGAAGGGCCACAGGTATAAAGCGCTaaa            >  1:1425410/1‑150 (MQ=255)
                                                                                                                                            ccAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAg           <  2:44007/150‑1 (MQ=255)
                                                                                                                                             cAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGt          >  1:1470919/1‑150 (MQ=255)
                                                                                                                                              aGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTATAGTTTTGAAGGGGCACTGGAATAAAGCGCTAAAGTg         >  1:46040/1‑150 (MQ=255)
                                                                                                                                               gTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTGa        >  1:440269/1‑150 (MQ=255)
                                                                                                                                               gTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTGa        >  1:1517257/1‑150 (MQ=255)
                                                                                                                                                ttCCGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTGaa       <  1:956946/150‑1 (MQ=255)
                                                                                                                                                  cagcagAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGGATAAAGCGCTAAAGTGAAat     >  2:936922/1‑150 (MQ=255)
                                                                                                                                                     cagAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTGAAATATc  <  1:1236141/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
TCGCTATTTTGCAGCCATATGGCATAAAGCGGCCGTGAAAGTGTTGTGCTATCGACAACGGTATGCAGGCCGCCTTTTTTACGCGCCCAGCTGACGGGTAGCCAGGTGCAACCATTAAGCATCGCAATCTGTTGCTGTGCCAGTTCAGCAGAACTGGTTGTCAGAATGGGCACTTCGTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTGAAATATC  >  NC_000913/3947186‑3947483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: