Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,965,396 | C→T | 5.3% | D79N (GAT→AAT) | rhlB ← | ATP‑dependent RNA helicase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,965,396 | 0 | C | T | 5.3% | 419.7 / 6.7 | 131 | D79N (GAT→AAT) | rhlB | ATP‑dependent RNA helicase |
Reads supporting (aligned to +/- strand): ref base C (64/59); new base T (3/4); total (68/63) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.12e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 |
Only 100 of 134 total aligned reads displayed.
CAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAA > NC_000913/3965247‑3965545 | gCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCgg < 2:1539379/150‑1 (MQ=255) gTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGc > 1:878532/1‑150 (MQ=255) cGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAg > 2:867078/1‑150 (MQ=255) cGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAg > 1:663952/1‑150 (MQ=255) cGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAg < 1:1266292/150‑1 (MQ=255) tAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGAAGCTTCCGCCAGCGGTTCAGCGGCGGCAAGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCCTGCGATTGCCGATCGCaagg < 2:826717/150‑4 (MQ=255) aGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATTGCCGATCGCaaggt < 1:1028170/150‑5 (MQ=255) gCCATCACCGCCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATTGGCAATCGCAGGATg < 2:739805/150‑1 (MQ=255) gCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATg > 2:1569739/1‑150 (MQ=255) gCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATg > 1:952646/1‑150 (MQ=255) gCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATg > 1:958808/1‑150 (MQ=255) gCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCCGCATGGATCTGCACGGCAAGTTAACGCGTCGGTGCCATAAATAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATg > 2:317842/1‑150 (MQ=255) tCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATgagag < 2:546918/150‑1 (MQ=255) caccacCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCTAGTTCACGCGTCGGTGACATAATTAAGGCCCGCTGCTGATTCACCTTGCGAGCGTCTATCTCAGGATgagaga > 2:1089307/1‑150 (MQ=255) caccacCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATgagaga > 2:1712194/1‑150 (MQ=255) ccaccGTAAGCCAGACCCAGGTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTGGGTGCCTTAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATgagagaga < 2:1616586/150‑1 (MQ=255) ccGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGagaaga > 1:965757/1‑150 (MQ=255) cGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGAt < 1:669907/150‑1 (MQ=255) aaGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAAt < 1:548414/150‑1 (MQ=255) cgCCAGCCCCAGCTTCAGGCCAGTCGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGGTAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATg < 2:1274975/149‑1 (MQ=255) aGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATg < 2:967472/150‑1 (MQ=255) aGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATg > 2:595178/1‑150 (MQ=255) aGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATg < 1:1716369/150‑1 (MQ=255) aGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATg > 1:1080280/1‑150 (MQ=255) gCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGa > 1:1603219/1‑150 (MQ=255) ccAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGaa > 2:1247169/1‑150 (MQ=255) ctgACGCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGaaa < 2:932430/148‑1 (MQ=255) cAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGaaa < 1:591325/150‑1 (MQ=255) gACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACg < 1:1493833/150‑1 (MQ=255) ccAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTg > 1:823873/1‑150 (MQ=255) aGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGAc > 2:1025097/1‑150 (MQ=255) aGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCAAGGCAAGTTCACGCGTCGTTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATTAGAGAGAAGATAATGAAACGTTGAc > 2:253553/1‑150 (MQ=255) gCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGGCGGTGCCAGAATTAAGGCACGCGGCAGCTTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACg > 2:786906/1‑150 (MQ=255) cAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCCCGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAg > 2:1128497/1‑150 (MQ=255) cAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAg > 2:422590/1‑150 (MQ=255) cAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAg < 2:57837/150‑1 (MQ=255) cAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAg < 2:599830/150‑1 (MQ=255) ccAGTAGCTTCCGCCAGCGGGTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGGCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAAc < 2:1055046/150‑1 (MQ=255) cAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACg > 2:114074/1‑150 (MQ=255) cAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACg < 2:878532/150‑1 (MQ=255) aGTAGCTTACGCAAGCGGTACTCCGTCGGCAAGGATCTGCACGGCCAGTTCACGCGTCGGTGAAATAATTAAGGCACGCGGCTGATTCACCTTGCGATAGGCATTCGGAGGATGAGAGAGAAGATAATGAAACGTTGACGAAAGTAACGc > 2:1076216/1‑150 (MQ=255) cGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGtttt > 1:503652/1‑150 (MQ=255) aGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCg > 1:1393358/1‑150 (MQ=255) tGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGt > 1:1542507/1‑150 (MQ=255) gTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTg < 1:226737/150‑1 (MQ=255) gCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCTCGTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAAGGTTGACGTTAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGcc < 1:1179903/150‑1 (MQ=255) gCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCGTCGTTTTCCCGGTACCGGTTTGCGcc < 1:44916/150‑1 (MQ=255) gCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGcc < 1:888929/150‑1 (MQ=255) gCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGcc < 1:132170/150‑1 (MQ=255) tGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGc > 1:78348/1‑150 (MQ=255) tGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGc < 1:789621/150‑1 (MQ=255) tGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGc > 2:569474/1‑150 (MQ=255) tGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGc > 1:1653189/1‑150 (MQ=255) tGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGc < 1:428660/150‑1 (MQ=255) tGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCAGGTACCGGTTTGCGCCTGc > 2:463830/1‑150 (MQ=255) gATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGccc < 2:1028292/150‑1 (MQ=255) cTGCACGGCAAGTTCACGCGGCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGc < 2:1201476/150‑1 (MQ=255) tGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCt > 1:1254418/1‑150 (MQ=255) tGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACAGGTTTGCGCCTGCCCGGCt > 2:797469/1‑150 (MQ=255) tGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGAATCACCTGGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGAAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCt < 2:831692/150‑1 (MQ=255) gCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTa < 1:1712194/150‑1 (MQ=255) aCGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACg > 1:1343060/1‑150 (MQ=255) gCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCa < 1:659820/150‑1 (MQ=255) gTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGAc > 2:610366/1‑150 (MQ=255) cACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCg > 2:1597540/1‑150 (MQ=255) cACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCg > 2:76856/1‑150 (MQ=255) cACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCg > 1:396668/1‑150 (MQ=255) aCGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGc > 1:1042064/1‑150 (MQ=255) cgcgTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCAGCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGcc < 1:867078/150‑1 (MQ=255) cgcgACGGCGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGcc < 1:180678/150‑1 (MQ=255) cgTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAg > 2:1531727/1‑150 (MQ=255) gTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGc > 2:1676313/1‑150 (MQ=255) ggTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTc < 1:499302/150‑1 (MQ=255) gCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGc < 2:1393358/150‑1 (MQ=255) cATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCgg > 2:891229/1‑150 (MQ=255) aaTTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAg > 1:1206063/1‑150 (MQ=255) aTTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAgg > 2:378479/1‑150 (MQ=255) aaGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGcc < 1:317842/150‑1 (MQ=255) aCGCGGCTGATTCCCCTTGCGATCGGCAATCGCAGGATGAGAGAGCAGATCATGAAACGGTGACGTAAGAAACGCCATCGGTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCCCGACCCGCCAGCGTCAGCGGAAGGGCCAGTGc < 1:1129429/150‑1 (MQ=255) aCGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGc < 2:1603219/150‑1 (MQ=255) gCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGaa > 1:1673926/1‑150 (MQ=255) gATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATgg > 1:573921/1‑150 (MQ=255) ttCACCTTGCGATCGGCTATCGCAGGATGAGAGAGAAGATAATGAACCTCTGACGTAAGAAACGCCATCGTTTTCCCGGTTCCGGTGAGCGCCGGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGc < 1:1142542/150‑1 (MQ=255) ttCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGc < 1:1521627/150‑1 (MQ=255) catcctGCGATTGCCGATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTCCCCGGCTACGACACGAACCGCCAGCGTCCGCGGAAGGGCCAGAGCCCGAATCGGcca > 1:259329/6‑148 (MQ=255) atcctGCGATTGCCGATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTa > 1:1697770/5‑150 (MQ=255) atcctGCGATTGCCGATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTa > 1:1604554/5‑150 (MQ=255) ccTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTAc > 2:1651828/1‑150 (MQ=255) ccTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATAGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTAc < 1:1128497/150‑1 (MQ=255) ttGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAg < 2:1299948/150‑1 (MQ=255) ttGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAg < 2:1132212/150‑1 (MQ=255) ttGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAg > 2:205074/1‑150 (MQ=255) tGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGt < 1:1386359/150‑1 (MQ=255) tGCGATCGGCAATCGCAGGATGAGAGAGAAGAAAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGt < 1:1185852/150‑1 (MQ=255) cGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTa < 1:1531727/150‑1 (MQ=255) cGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTa < 2:319383/150‑1 (MQ=255) gATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAACCGCCATCGGTTTCCCGGTACCGGTTTGCGCATGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCATGAATGGGAGTACAGTTAt > 2:263144/1‑150 (MQ=255) gATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTAt > 2:332497/1‑150 (MQ=255) aTCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAACCGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCTTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATg < 1:32434/150‑1 (MQ=255) cGGCAATCGCAGGATGAGAGAGAAGATAATGAAACTTAGACGTAAGAAACGCCATAGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCCGCGGAAGGGCCAGTGCATGAATGGGCGTACAGTTATGaa > 2:1162801/1‑150 (MQ=255) | CAGCTGTTTGTCGTAGCCATCACCACCGTAAGCCAGACCCAGCTTCAGGCCAGTAGCTTCCGCCAGCGGTTCTGCGTCGGCATGGATCTGCACGGCAAGTTCACGCGTCGGTGCCATAATTAAGGCACGCGGCTGATTCACCTTGCGATCGGCAATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAA > NC_000913/3965247‑3965545 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |