Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,969,434 A→C 6.2% D135A (GAT→GCT) ‡ wzzE → Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,969,4340AC 6.2% 302.0 / 8.5 108D135A (GAT→GCT) ‡wzzEEntobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
Reads supporting (aligned to +/- strand):  ref base A (47/54);  new base C (4/3);  total (51/57)
Fisher's exact test for biased strand distribution p-value = 7.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.90e-01

Only 100 of 110 total aligned reads displayed.

TTCAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGC  >  NC_000913/3969285‑3969583
                                                                                                                                                     |                                                                                                                                                     
 tCAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGAt                                                                                                                                                      <  2:1558784/150‑1 (MQ=255)
   aaaCATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGGAACAGCAAAGCCGATgc                                                                                                                                                    <  1:420745/150‑1 (MQ=255)
      cATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATgcggc                                                                                                                                                 >  1:1264002/1‑150 (MQ=255)
      cATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGACTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACACGCAGAGAGATCTGGCTGAAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATgaggc                                                                                                                                                 >  1:1234038/1‑150 (MQ=255)
         ggCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGtt                                                                                                                                              <  2:1287838/150‑1 (MQ=255)
            ttCTGCCGACCAACCATCGGGCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTGCTGGCTGCAACCCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGAAGCGGCGTTGCt                                                                                                                                           <  2:77159/150‑1 (MQ=255)
              atGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTgg                                                                                                                                         <  2:1266740/149‑1 (MQ=255)
               tGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAACGCCGCATCGGCTTTGCTgtt                                                                                                                                        <  2:1256125/150‑3 (MQ=255)
                gCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGAt                                                                                                                                       >  1:1007763/1‑150 (MQ=255)
                gCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGAt                                                                                                                                       >  1:641374/1‑150 (MQ=255)
                gCCGACCAACCATCGCTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAACGCCGCATCGGCTTTGCTgttg                                                                                                                                       <  1:358215/150‑4 (MQ=255)
                 ccGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAACGCCGCATCGGCTTTGCTgttgc                                                                                                                                      <  2:607127/150‑5 (MQ=255)
                 ccGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATg                                                                                                                                      >  2:666687/1‑150 (MQ=255)
                 ccGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTCGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATg                                                                                                                                      <  1:148533/150‑1 (MQ=255)
                      caaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATg                                                                                                                                 >  1:1627469/1‑150 (MQ=255)
                      caaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATg                                                                                                                                 >  2:1436056/1‑150 (MQ=255)
                      caaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATg                                                                                                                                 >  1:912188/1‑150 (MQ=255)
                       aaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGa                                                                                                                                >  1:1516289/1‑150 (MQ=255)
                        accaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGAt                                                                                                                               >  1:709948/1‑150 (MQ=255)
                         ccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGAtt                                                                                                                              <  2:1232439/150‑1 (MQ=255)
                         ccaTCGGTCATGGACGAAGCCAATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGAATATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGAtt                                                                                                                              <  1:205358/150‑1 (MQ=255)
                          caTCGGTCATGTACGAAGCCTATAAAGAGATTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGGAAACCGACTATTACAAACAGCTGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTa                                                                                                                             <  1:305232/150‑1 (MQ=255)
                          caTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTa                                                                                                                             <  1:1373253/150‑1 (MQ=255)
                          caTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTa                                                                                                                             <  1:831425/150‑1 (MQ=255)
                          caTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGGGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTa                                                                                                                             <  1:1072939/150‑1 (MQ=255)
                                tCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAacaaca                                                                                                                       >  2:1497844/1‑150 (MQ=255)
                                   tGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATcc                                                                                                                    <  2:1443751/150‑1 (MQ=255)
                                           gCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATc                                                                                                            <  2:1312457/150‑1 (MQ=255)
                                             cTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATccc                                                                                                          <  1:1380507/150‑1 (MQ=255)
                                                 aaaGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGtt                                                                                                                <  1:1126823/140‑1 (MQ=255)
                                                 aaaGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGtt                                                                                                                >  2:1126823/1‑140 (MQ=255)
                                                 aaaGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGga                                                                                                      >  1:713913/1‑150 (MQ=255)
                                                     aggttGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAGCCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGCTGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGCCt                                                                                                  >  2:1045128/4‑150 (MQ=255)
                                                       tttGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACttt                                                                                                >  2:719365/1‑150 (MQ=255)
                                                          gTTATGCAACTGGCCTCGTGGGATACGCGCTGAGATTTCTGGCTGCAAACCGCCTATTACTAACAGCGGCGGGTGGGCAAGAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTAcc                                                                                             <  1:586026/150‑1 (MQ=255)
                                                          gTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTAcc                                                                                             <  1:1576902/150‑1 (MQ=255)
                                                            tATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTCCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCcg                                                                                           <  2:1669689/150‑1 (MQ=255)
                                                             aTGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCcgc                                                                                          >  2:44562/1‑150 (MQ=255)
                                                                 aaCTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCgg                                                                                      >  1:343243/1‑150 (MQ=255)
                                                                 aaCTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCgg                                                                                      <  1:600827/150‑1 (MQ=255)
                                                                     ggCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGTTGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCaa                                                                                  >  1:73681/1‑150 (MQ=255)
                                                                       ccTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTTGGCAACAGCATAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATg                                                                                <  1:876963/150‑1 (MQ=255)
                                                                         tCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCTACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGAc                                                                              <  2:687388/150‑1 (MQ=255)
                                                                           ttGGTATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATTGTTGGCAACAGCAAAGCCGATGCGGCGTTGATGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGTTCAATGACAg                                                                            <  1:823212/149‑1 (MQ=255)
                                                                             gggATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTTGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCg                                                                          <  2:1688277/150‑1 (MQ=255)
                                                                             gggATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCg                                                                          >  2:845616/1‑150 (MQ=255)
                                                                                aTACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCTGTCATTGACcgcgcgg                                                                       <  2:521599/150‑4 (MQ=255)
                                                                                   cccGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGc                                                                    <  1:719365/150‑1 (MQ=255)
                                                                                    ccGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTATCAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCt                                                                   >  2:1137364/1‑150 (MQ=255)
                                                                                    ccGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTATCAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCt                                                                   <  1:62423/150‑1 (MQ=255)
                                                                                      gCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTa                                                                 <  1:16408/150‑1 (MQ=255)
                                                                                      gCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTa                                                                 >  2:1434352/1‑150 (MQ=255)
                                                                                         gagagaTCTGGCTGCAAACCGACTATTACAAACAGCGGATCGTGGGCAACAGCAAAGCCGATGCGGCCTTGATGGATGAAATGATTTACAACATCCAGTTTATCCCCGGAGACTTTACCCCCGCGGTCAATGACAGCGTGAAGCTTAttt                                                              >  1:1236021/1‑149 (MQ=255)
                                                                                         gagagTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTg                                                              <  2:1627469/150‑1 (MQ=255)
                                                                                         gagagTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTg                                                              <  2:709948/150‑1 (MQ=255)
                                                                                           gagTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTATACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGcc                                                            >  2:306011/1‑150 (MQ=255)
                                                                                                ctgtccgCAACCCCACTATTACAAACCGCGGATCGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTCATCCCCGGAGACTTTACCCGCGCGCGCAATGTCAGCGTGAAGCTTATTGCCGAAAc                                                       <  1:672272/144‑1 (MQ=255)
                                                                                                cTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGTGGTCAATGACAGCGTGAAGCTTATTGCCGAAAc                                                       >  2:291396/1‑150 (MQ=255)
                                                                                                cTGGCTGCAAACCGACTATTACAAACAGCGGATGGTCCGCAACAGCAAAGCCGAAGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATACCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTCAAGCTTATAGCAGAAAc                                                       >  2:417612/1‑150 (MQ=255)
                                                                                                 tGGCTGCAAACCGACTATTACAAACAGCGGATGTTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAAcc                                                      <  2:261794/150‑1 (MQ=255)
                                                                                                    cTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACcgcg                                                   <  1:952542/150‑1 (MQ=255)
                                                                                                     tGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCgcgc                                                  >  1:780995/1‑150 (MQ=255)
                                                                                                      gCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGcc                                                 <  2:743088/150‑1 (MQ=255)
                                                                                                      gCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATAATTAACAACATCCAGTTTATCCCCGGAGACTTTCCCCGCGCGGTCAATGCCAGCGTGAAGCTTTTTGCCGAAACCGCGcc                                                 >  2:295260/1‑150 (MQ=255)
                                                                                                        aaaCCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTg                                               >  2:365574/1‑150 (MQ=255)
                                                                                                         aaCCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGa                                              >  1:1493615/1‑150 (MQ=255)
                                                                                                                   tACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTAACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAAc                                    <  1:666687/150‑1 (MQ=255)
                                                                                                                    aCAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGTGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAAcc                                   <  1:291396/150‑1 (MQ=255)
                                                                                                                    aCAAACAGCGGATGGTGCGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACCACAACCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAAcc                                   <  1:62479/150‑1 (MQ=255)
                                                                                                                      aaaCAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGATGCTAATAACCTg                                 <  1:199283/150‑1 (MQ=255)
                                                                                                                       aaCAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGt                                >  2:238394/1‑150 (MQ=255)
                                                                                                                          aGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTAc                             >  2:785224/1‑150 (MQ=255)
                                                                                                                           gCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACg                            <  2:424344/150‑1 (MQ=255)
                                                                                                                             ggATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTc                          <  2:337089/150‑1 (MQ=255)
                                                                                                                             ggATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTc                          <  1:365574/150‑1 (MQ=255)
                                                                                                                             ggATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTc                          <  2:1342358/150‑1 (MQ=255)
                                                                                                                             ggATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAAAATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTc                          <  2:1234038/150‑1 (MQ=255)
                                                                                                                              gATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATCATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGAAAGCGTGAAGCTTATTGCCGAAACCTCGCCTGACGCTAATAACCTGTTACGTCa                         >  1:302812/1‑150 (MQ=255)
                                                                                                                                tggtggGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGt                       >  2:1538146/1‑150 (MQ=255)
                                                                                                                                  gtggGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCTGTAt                     >  1:495190/1‑150 (MQ=255)
                                                                                                                                   tggGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATg                    >  2:536945/1‑150 (MQ=255)
                                                                                                                                     ggCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGACACCGCGCCTCACGCTAATAACCTGTTACGTCAGTATGtt                  >  1:1074995/1‑150 (MQ=255)
                                                                                                                                     ggCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGtt                  >  2:1726614/1‑150 (MQ=255)
                                                                                                                                      gCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTg                 <  2:1493615/150‑1 (MQ=255)
                                                                                                                                       cAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGc                >  1:849675/1‑150 (MQ=255)
                                                                                                                                       cAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGc                <  2:1516289/150‑1 (MQ=255)
                                                                                                                                       cAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGc                <  2:1174200/150‑1 (MQ=255)
                                                                                                                                         ccAGCAACGCCGCATCGGCTTTGCTGGATGAAATGATTAACACCATACAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCtt              >  1:1195906/2‑150 (MQ=255)
                                                                                                                                          cAGCAACGCCGCATCGGCTTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCttt             >  2:1682611/1‑150 (MQ=255)
                                                                                                                                          cAGCAACGCCGCATCGGCTTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCttt             >  2:1342866/1‑150 (MQ=255)
                                                                                                                                           aGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCtttt            <  1:291909/150‑1 (MQ=255)
                                                                                                                                            gCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTg           <  2:73681/150‑1 (MQ=255)
                                                                                                                                            gCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTg           >  2:773124/1‑150 (MQ=255)
                                                                                                                                             cAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTgc          <  1:536945/150‑1 (MQ=255)
                                                                                                                                               aaGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTgcca        <  1:477052/150‑1 (MQ=255)
                                                                                                                                               aaGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTgcca        >  1:581361/1‑150 (MQ=255)
                                                                                                                                                  ccGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTgccagcc     >  1:151726/1‑150 (MQ=255)
                                                                                                                                                   cGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTgccagcca    <  1:1497844/150‑1 (MQ=255)
                                                                                                                                                   cGATGCGGCGTTGCTGGATGAAATCATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCGGTTACGTCAGTATGTTGCTTTTgccagccc    >  2:1699031/1‑149 (MQ=255)
                                                                                                                                                     aTGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTGCcagccagc  >  1:609865/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTCAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGC  >  NC_000913/3969285‑3969583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑