Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,969,435 T→A 6.9% D135E (GAT→GAA) ‡ wzzE → Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,969,4350TA 6.9% 297.7 / 7.0 108D135E (GAT→GAA) ‡wzzEEntobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
Reads supporting (aligned to +/- strand):  ref base T (48/52);  new base A (4/4);  total (52/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

TCAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGC  >  NC_000913/3969286‑3969583
                                                                                                                                                     |                                                                                                                                                    
tCAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGAt                                                                                                                                                      <  2:1558784/150‑1 (MQ=255)
  aaaCATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGGAACAGCAAAGCCGATgc                                                                                                                                                    <  1:420745/150‑1 (MQ=255)
     cATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATgcggc                                                                                                                                                 >  1:1264002/1‑150 (MQ=255)
     cATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGACTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACACGCAGAGAGATCTGGCTGAAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATgaggc                                                                                                                                                 >  1:1234038/1‑150 (MQ=255)
        ggCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGtt                                                                                                                                              <  2:1287838/150‑1 (MQ=255)
           ttCTGCCGACCAACCATCGGGCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTGCTGGCTGCAACCCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGAAGCGGCGTTGCt                                                                                                                                           <  2:77159/150‑1 (MQ=255)
           gtCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCt                                                                                                                                           <  2:620731/149‑1 (MQ=255)
             atGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTgg                                                                                                                                         <  2:1266740/149‑1 (MQ=255)
              tGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAACGCCGCATCGGCTTTGCTgtt                                                                                                                                        <  2:1256125/150‑3 (MQ=255)
               gCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATCCCCGCAGAGAGTTCTGGCTGCAAACCGCCTATTACAAACAGCGGAGGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGAt                                                                                                                                       >  1:521599/1‑150 (MQ=255)
               gCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGAt                                                                                                                                       >  1:1007763/1‑150 (MQ=255)
               gCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGAt                                                                                                                                       >  1:641374/1‑150 (MQ=255)
               gCCGACCAACCATCGCTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAACGCCGCATCGGCTTTGCTgttg                                                                                                                                       <  1:358215/150‑4 (MQ=255)
                ccGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATg                                                                                                                                      >  2:666687/1‑150 (MQ=255)
                ccGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTCGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATg                                                                                                                                      <  1:148533/150‑1 (MQ=255)
                     caaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATg                                                                                                                                 >  1:1627469/1‑150 (MQ=255)
                     caaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATg                                                                                                                                 >  2:1436056/1‑150 (MQ=255)
                     caaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATg                                                                                                                                 >  1:912188/1‑150 (MQ=255)
                      aaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGa                                                                                                                                >  1:1516289/1‑150 (MQ=255)
                      aaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGa                                                                                                                                >  1:921109/1‑150 (MQ=255)
                       accaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGAt                                                                                                                               >  1:709948/1‑150 (MQ=255)
                        ccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGAtt                                                                                                                              <  2:1232439/150‑1 (MQ=255)
                        ccaTCGGTCATGGACGAAGCCAATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGAATATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGAtt                                                                                                                              <  1:205358/150‑1 (MQ=255)
                         caTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTa                                                                                                                             <  1:1373253/150‑1 (MQ=255)
                         caTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTa                                                                                                                             <  1:831425/150‑1 (MQ=255)
                         caTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGGGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTa                                                                                                                             <  1:1072939/150‑1 (MQ=255)
                                  tGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATcc                                                                                                                    <  2:1443751/150‑1 (MQ=255)
                                  tGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATcc                                                                                                                    >  2:1306442/1‑150 (MQ=255)
                                          gCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATc                                                                                                            <  2:1312457/150‑1 (MQ=255)
                                            cTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATccc                                                                                                          <  1:1380507/150‑1 (MQ=255)
                                             tataAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATcccc                                                                                                         >  2:291909/1‑150 (MQ=255)
                                                aaaGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGtt                                                                                                                <  1:1126823/140‑1 (MQ=255)
                                                aaaGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGtt                                                                                                                >  2:1126823/1‑140 (MQ=255)
                                                aaaGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGga                                                                                                      >  1:713913/1‑150 (MQ=255)
                                                    aggttGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAGCCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGCTGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGCCt                                                                                                  >  2:1045128/4‑150 (MQ=255)
                                                      tttGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACttt                                                                                                >  2:719365/1‑150 (MQ=255)
                                                         gTTATGCAACTGGCCTCGTGGGATACGCGCTGAGATTTCTGGCTGCAAACCGCCTATTACTAACAGCGGCGGGTGGGCAAGAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTAcc                                                                                             <  1:586026/150‑1 (MQ=255)
                                                         gTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTAcc                                                                                             <  1:1576902/150‑1 (MQ=255)
                                                          ttATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACATACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTAccc                                                                                            >  1:281618/1‑150 (MQ=255)
                                                           tATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTCCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCcg                                                                                           <  2:1669689/150‑1 (MQ=255)
                                                            aTGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCcgc                                                                                          >  2:44562/1‑150 (MQ=255)
                                                                aaCTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCgg                                                                                      >  1:343243/1‑150 (MQ=255)
                                                                aaCTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCgg                                                                                      <  1:600827/150‑1 (MQ=255)
                                                                    ggCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGTTGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCaa                                                                                  >  1:73681/1‑150 (MQ=255)
                                                                      ccTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTTGGCAACAGCATAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATg                                                                                <  1:876963/150‑1 (MQ=255)
                                                                        tCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCTACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGAc                                                                              <  2:687388/150‑1 (MQ=255)
                                                                            gggATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTTGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCg                                                                          <  2:1688277/150‑1 (MQ=255)
                                                                            gggATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCg                                                                          >  2:845616/1‑150 (MQ=255)
                                                                               aTACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCTGTCATTGACcgcgcgg                                                                       <  2:521599/150‑4 (MQ=255)
                                                                                  cccGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGc                                                                    <  1:719365/150‑1 (MQ=255)
                                                                                   ccGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTATCAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCt                                                                   >  2:1137364/1‑150 (MQ=255)
                                                                                   ccGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTATCAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCt                                                                   <  1:62423/150‑1 (MQ=255)
                                                                                     gCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTa                                                                 <  1:16408/150‑1 (MQ=255)
                                                                                     gCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTa                                                                 >  2:1434352/1‑150 (MQ=255)
                                                                                        gagagaTCTGGCTGCAAACCGACTATTACAAACAGCGGATCGTGGGCAACAGCAAAGCCGATGCGGCCTTGATGGATGAAATGATTTACAACATCCAGTTTATCCCCGGAGACTTTACCCCCGCGGTCAATGACAGCGTGAAGCTTAttt                                                              >  1:1236021/1‑149 (MQ=255)
                                                                                        gagagTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTg                                                              <  2:709948/150‑1 (MQ=255)
                                                                                        gagagTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTg                                                              <  2:1627469/150‑1 (MQ=255)
                                                                                          gagTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGcc                                                            >  1:691950/1‑150 (MQ=255)
                                                                                          gagTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTATACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGcc                                                            >  2:306011/1‑150 (MQ=255)
                                                                                               ctgtccgCAACCCCACTATTACAAACCGCGGATCGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTCATCCCCGGAGACTTTACCCGCGCGCGCAATGTCAGCGTGAAGCTTATTGCCGAAAc                                                       <  1:672272/144‑1 (MQ=255)
                                                                                               cTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGTGGTCAATGACAGCGTGAAGCTTATTGCCGAAAc                                                       >  2:291396/1‑150 (MQ=255)
                                                                                               cTGGCTGCAAACCGACTATTACAAACAGCGGATGGTCCGCAACAGCAAAGCCGAAGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATACCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTCAAGCTTATAGCAGAAAc                                                       >  2:417612/1‑150 (MQ=255)
                                                                                                   cTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACcgcg                                                   <  2:1554642/150‑1 (MQ=255)
                                                                                                   cTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACcgcg                                                   <  1:952542/150‑1 (MQ=255)
                                                                                                    tGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCgcgc                                                  >  1:780995/1‑150 (MQ=255)
                                                                                                     gCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGcc                                                 <  2:743088/150‑1 (MQ=255)
                                                                                                     gCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATAATTAACAACATCCAGTTTATCCCCGGAGACTTTCCCCGCGCGGTCAATGCCAGCGTGAAGCTTTTTGCCGAAACCGCGcc                                                 >  2:295260/1‑150 (MQ=255)
                                                                                                       aaaCCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTg                                               >  2:365574/1‑150 (MQ=255)
                                                                                                        aaCCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGa                                              >  1:1493615/1‑150 (MQ=255)
                                                                                                                  tACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTAACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAAc                                    <  1:666687/150‑1 (MQ=255)
                                                                                                                   aCAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGTGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAAcc                                   <  1:291396/150‑1 (MQ=255)
                                                                                                                   aCAAACAGCGGATGGTGCGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACCACAACCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAAcc                                   <  1:62479/150‑1 (MQ=255)
                                                                                                                     aaaCAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGATGCTAATAACCTg                                 <  1:199283/150‑1 (MQ=255)
                                                                                                                      aaCAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGt                                >  2:238394/1‑150 (MQ=255)
                                                                                                                         aGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTAc                             >  2:785224/1‑150 (MQ=255)
                                                                                                                          gCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACg                            <  2:424344/150‑1 (MQ=255)
                                                                                                                            ggATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTc                          <  1:365574/150‑1 (MQ=255)
                                                                                                                            ggATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTc                          <  2:1342358/150‑1 (MQ=255)
                                                                                                                            ggATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTc                          <  2:337089/150‑1 (MQ=255)
                                                                                                                             gATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATCATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGAAAGCGTGAAGCTTATTGCCGAAACCTCGCCTGACGCTAATAACCTGTTACGTCa                         >  1:302812/1‑150 (MQ=255)
                                                                                                                               tggtggGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGt                       >  2:1538146/1‑150 (MQ=255)
                                                                                                                                 gtggGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCTGTAt                     >  1:495190/1‑150 (MQ=255)
                                                                                                                                  tggGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATg                    >  2:536945/1‑150 (MQ=255)
                                                                                                                                    ggCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGACACCGCGCCTCACGCTAATAACCTGTTACGTCAGTATGtt                  >  1:1074995/1‑150 (MQ=255)
                                                                                                                                    ggCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGtt                  >  2:1726614/1‑150 (MQ=255)
                                                                                                                                     gCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTg                 <  2:1493615/150‑1 (MQ=255)
                                                                                                                                      cAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGc                <  2:1516289/150‑1 (MQ=255)
                                                                                                                                      cAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGc                <  2:1174200/150‑1 (MQ=255)
                                                                                                                                      cAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGc                >  1:849675/1‑150 (MQ=255)
                                                                                                                                        ccAGCAACGCCGCATCGGCTTTGCTGGATGAAATGATTAACACCATACAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCtt              >  1:1195906/2‑150 (MQ=255)
                                                                                                                                         cAGCAACGCCGCATCGGCTTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCttt             >  2:1682611/1‑150 (MQ=255)
                                                                                                                                         cAGCAACGCCGCATCGGCTTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCttt             >  2:1342866/1‑150 (MQ=255)
                                                                                                                                          aGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCtttt            <  1:291909/150‑1 (MQ=255)
                                                                                                                                           gCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTg           <  2:73681/150‑1 (MQ=255)
                                                                                                                                           gCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTg           >  2:773124/1‑150 (MQ=255)
                                                                                                                                            cAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTgc          <  1:536945/150‑1 (MQ=255)
                                                                                                                                              aaGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTgcca        <  1:477052/150‑1 (MQ=255)
                                                                                                                                              aaGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTgcca        >  1:581361/1‑150 (MQ=255)
                                                                                                                                                 ccGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTgccagcc     >  1:151726/1‑150 (MQ=255)
                                                                                                                                                  cGATGCGGCGTTGCTGGATGAAATCATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCGGTTACGTCAGTATGTTGCTTTTgccagccc    >  2:1699031/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGC  >  NC_000913/3969286‑3969583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: