Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,969,436 G→T 5.7% A136S (GCG→TCG)  wzzE → Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,969,4360GT 5.7% 310.4 / 7.3 105A136S (GCG→TCG) wzzEEntobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
Reads supporting (aligned to +/- strand):  ref base G (47/52);  new base T (3/3);  total (50/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.84e-01

Only 100 of 109 total aligned reads displayed.

AAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGT  >  NC_000913/3969288‑3969585
                                                                                                                                                    |                                                                                                                                                     
aaaCATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGGAACAGCAAAGCCGATgc                                                                                                                                                      <  1:420745/150‑1 (MQ=255)
   cATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATgcggc                                                                                                                                                   >  1:1264002/1‑150 (MQ=255)
   cATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGACTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACACGCAGAGAGATCTGGCTGAAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATgaggc                                                                                                                                                   >  1:1234038/1‑150 (MQ=255)
      ggCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGtt                                                                                                                                                <  2:1287838/150‑1 (MQ=255)
         ttCTGCCGACCAACCATCGGGCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTGCTGGCTGCAACCCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGAAGCGGCGTTGCt                                                                                                                                             <  2:77159/150‑1 (MQ=255)
         gtCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCt                                                                                                                                             <  2:620731/149‑1 (MQ=255)
           atGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTgg                                                                                                                                           <  2:1266740/149‑1 (MQ=255)
            tGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAACGCCGCATCGGCTTTGCTgtt                                                                                                                                          <  2:1256125/150‑3 (MQ=255)
             gCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATCCCCGCAGAGAGTTCTGGCTGCAAACCGCCTATTACAAACAGCGGAGGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGAt                                                                                                                                         >  1:521599/1‑150 (MQ=255)
             gCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGAt                                                                                                                                         >  1:1007763/1‑150 (MQ=255)
             gCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGAt                                                                                                                                         >  1:641374/1‑150 (MQ=255)
             gCCGACCAACCATCGCTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAACGCCGCATCGGCTTTGCTgttg                                                                                                                                         <  1:358215/150‑4 (MQ=255)
              ccGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATg                                                                                                                                        >  2:666687/1‑150 (MQ=255)
              ccGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTCGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATg                                                                                                                                        <  1:148533/150‑1 (MQ=255)
                   caaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATg                                                                                                                                   >  1:912188/1‑150 (MQ=255)
                   caaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATg                                                                                                                                   >  2:1436056/1‑150 (MQ=255)
                   caaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATg                                                                                                                                   >  1:1627469/1‑150 (MQ=255)
                    aaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGa                                                                                                                                  >  1:1516289/1‑150 (MQ=255)
                    aaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGa                                                                                                                                  >  1:921109/1‑150 (MQ=255)
                     accaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGAt                                                                                                                                 >  1:709948/1‑150 (MQ=255)
                      ccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGAtt                                                                                                                                <  2:1232439/150‑1 (MQ=255)
                      ccaTCGGTCATGGACGAAGCCAATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGAATATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGAtt                                                                                                                                <  1:205358/150‑1 (MQ=255)
                       caTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTa                                                                                                                               <  1:1373253/150‑1 (MQ=255)
                       caTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTa                                                                                                                               <  1:831425/150‑1 (MQ=255)
                       caTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGGGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTa                                                                                                                               <  1:1072939/150‑1 (MQ=255)
                             tCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAacaaca                                                                                                                         >  2:1497844/1‑150 (MQ=255)
                                tGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATcc                                                                                                                      <  2:1443751/150‑1 (MQ=255)
                                tGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATcc                                                                                                                      >  2:1306442/1‑150 (MQ=255)
                                        gCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATc                                                                                                              <  2:1312457/150‑1 (MQ=255)
                                          cTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATccc                                                                                                            <  1:1380507/150‑1 (MQ=255)
                                           tataAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATcccc                                                                                                           >  2:291909/1‑150 (MQ=255)
                                              aaaGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGtt                                                                                                                  <  1:1126823/140‑1 (MQ=255)
                                              aaaGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGtt                                                                                                                  >  2:1126823/1‑140 (MQ=255)
                                              aaaGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGga                                                                                                        >  1:713913/1‑150 (MQ=255)
                                                  aggttGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAGCCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGCTGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGCCt                                                                                                    >  2:1045128/4‑150 (MQ=255)
                                                    tttGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACttt                                                                                                  >  2:719365/1‑150 (MQ=255)
                                                       gTTATGCAACTGGCCTCGTGGGATACGCGCTGAGATTTCTGGCTGCAAACCGCCTATTACTAACAGCGGCGGGTGGGCAAGAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTAcc                                                                                               <  1:586026/150‑1 (MQ=255)
                                                       gTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTAcc                                                                                               <  1:1576902/150‑1 (MQ=255)
                                                        ttATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACATACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTAccc                                                                                              >  1:281618/1‑150 (MQ=255)
                                                         tATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTCCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCcg                                                                                             <  2:1669689/150‑1 (MQ=255)
                                                          aTGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCcgc                                                                                            >  2:44562/1‑150 (MQ=255)
                                                              aaCTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCgg                                                                                        >  1:343243/1‑150 (MQ=255)
                                                              aaCTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCgg                                                                                        <  1:600827/150‑1 (MQ=255)
                                                                  ggCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGTTGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCaa                                                                                    >  1:73681/1‑150 (MQ=255)
                                                                    ccTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTTGGCAACAGCATAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATg                                                                                  <  1:876963/150‑1 (MQ=255)
                                                                      tCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCTACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGAc                                                                                <  2:687388/150‑1 (MQ=255)
                                                                          gggATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTTGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCg                                                                            <  2:1688277/150‑1 (MQ=255)
                                                                          gggATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCg                                                                            >  2:845616/1‑150 (MQ=255)
                                                                             aTACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCTGTCATTGACcgcgcgg                                                                         <  2:521599/150‑4 (MQ=255)
                                                                                cccGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGc                                                                      <  1:719365/150‑1 (MQ=255)
                                                                                 ccGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTATCAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCt                                                                     >  2:1137364/1‑150 (MQ=255)
                                                                                 ccGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTATCAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCt                                                                     <  1:62423/150‑1 (MQ=255)
                                                                                   gCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTa                                                                   <  1:16408/150‑1 (MQ=255)
                                                                                   gCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTa                                                                   >  2:1434352/1‑150 (MQ=255)
                                                                                      gagagaTCTGGCTGCAAACCGACTATTACAAACAGCGGATCGTGGGCAACAGCAAAGCCGATGCGGCCTTGATGGATGAAATGATTTACAACATCCAGTTTATCCCCGGAGACTTTACCCCCGCGGTCAATGACAGCGTGAAGCTTAttt                                                                >  1:1236021/1‑149 (MQ=255)
                                                                                      gagagTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTg                                                                <  2:709948/150‑1 (MQ=255)
                                                                                      gagagTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTg                                                                <  2:1627469/150‑1 (MQ=255)
                                                                                        gagTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGcc                                                              >  1:691950/1‑150 (MQ=255)
                                                                                        gagTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTATACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGcc                                                              >  2:306011/1‑150 (MQ=255)
                                                                                             ctgtccgCAACCCCACTATTACAAACCGCGGATCGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTCATCCCCGGAGACTTTACCCGCGCGCGCAATGTCAGCGTGAAGCTTATTGCCGAAAc                                                         <  1:672272/144‑1 (MQ=255)
                                                                                             cTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGTGGTCAATGACAGCGTGAAGCTTATTGCCGAAAc                                                         >  2:291396/1‑150 (MQ=255)
                                                                                             cTGGCTGCAAACCGACTATTACAAACAGCGGATGGTCCGCAACAGCAAAGCCGAAGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATACCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTCAAGCTTATAGCAGAAAc                                                         >  2:417612/1‑150 (MQ=255)
                                                                                                 cTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACcgcg                                                     <  2:1554642/150‑1 (MQ=255)
                                                                                                 cTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACcgcg                                                     <  1:952542/150‑1 (MQ=255)
                                                                                                  tGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCgcgc                                                    >  1:780995/1‑150 (MQ=255)
                                                                                                   gCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGcc                                                   <  2:743088/150‑1 (MQ=255)
                                                                                                   gCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATAATTAACAACATCCAGTTTATCCCCGGAGACTTTCCCCGCGCGGTCAATGCCAGCGTGAAGCTTTTTGCCGAAACCGCGcc                                                   >  2:295260/1‑150 (MQ=255)
                                                                                                     aaaCCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTg                                                 >  2:365574/1‑150 (MQ=255)
                                                                                                      aaCCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGa                                                >  1:1493615/1‑150 (MQ=255)
                                                                                                                tACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTAACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAAc                                      <  1:666687/150‑1 (MQ=255)
                                                                                                                 aCAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGTGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAAcc                                     <  1:291396/150‑1 (MQ=255)
                                                                                                                 aCAAACAGCGGATGGTGCGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACCACAACCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAAcc                                     <  1:62479/150‑1 (MQ=255)
                                                                                                                   aaaCAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGATGCTAATAACCTg                                   <  1:199283/150‑1 (MQ=255)
                                                                                                                    aaCAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGt                                  >  2:238394/1‑150 (MQ=255)
                                                                                                                       aGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTAc                               >  2:785224/1‑150 (MQ=255)
                                                                                                                        gCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACg                              <  2:424344/150‑1 (MQ=255)
                                                                                                                          ggATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTc                            <  2:1342358/150‑1 (MQ=255)
                                                                                                                          ggATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTc                            <  1:365574/150‑1 (MQ=255)
                                                                                                                          ggATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTc                            <  2:337089/150‑1 (MQ=255)
                                                                                                                           gATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATCATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGAAAGCGTGAAGCTTATTGCCGAAACCTCGCCTGACGCTAATAACCTGTTACGTCa                           >  1:302812/1‑150 (MQ=255)
                                                                                                                             tggtggGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGt                         >  2:1538146/1‑150 (MQ=255)
                                                                                                                               gtggGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCTGTAt                       >  1:495190/1‑150 (MQ=255)
                                                                                                                                tggGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATg                      >  2:536945/1‑150 (MQ=255)
                                                                                                                                  ggCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGACACCGCGCCTCACGCTAATAACCTGTTACGTCAGTATGtt                    >  1:1074995/1‑150 (MQ=255)
                                                                                                                                  ggCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGtt                    >  2:1726614/1‑150 (MQ=255)
                                                                                                                                    cAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGc                  <  2:1516289/150‑1 (MQ=255)
                                                                                                                                    cAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGc                  <  2:1174200/150‑1 (MQ=255)
                                                                                                                                    cAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGc                  >  1:849675/1‑150 (MQ=255)
                                                                                                                                      ccAGCAACGCCGCATCGGCTTTGCTGGATGAAATGATTAACACCATACAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCtt                >  1:1195906/2‑150 (MQ=255)
                                                                                                                                       cAGCAACGCCGCATCGGCTTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCttt               >  2:1682611/1‑150 (MQ=255)
                                                                                                                                       cAGCAACGCCGCATCGGCTTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCttt               >  2:1342866/1‑150 (MQ=255)
                                                                                                                                        aGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCtttt              <  1:291909/150‑1 (MQ=255)
                                                                                                                                         gCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTg             <  2:73681/150‑1 (MQ=255)
                                                                                                                                         gCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTg             >  2:773124/1‑150 (MQ=255)
                                                                                                                                          cAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTgc            <  1:536945/150‑1 (MQ=255)
                                                                                                                                            aaGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTgcca          <  1:477052/150‑1 (MQ=255)
                                                                                                                                            aaGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTgcca          >  1:581361/1‑150 (MQ=255)
                                                                                                                                               ccGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTgccagcc       >  1:151726/1‑150 (MQ=255)
                                                                                                                                                cGATGCGGCGTTGCTGGATGAAATCATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCGGTTACGTCAGTATGTTGCTTTTgccagccc      >  2:1699031/1‑149 (MQ=255)
                                                                                                                                                    gcggcgTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGt  >  2:1493419/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGT  >  NC_000913/3969288‑3969585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: