Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,969,441 G→T 5.4% A137A (GCG→GCT wzzE → Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,969,4410GT 5.4% 316.2 / 4.3 105A137A (GCG→GCTwzzEEntobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
Reads supporting (aligned to +/- strand):  ref base G (46/52);  new base T (3/3);  total (50/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 108 total aligned reads displayed.

GGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGTG  >  NC_000913/3969294‑3969586
                                                                                                                                                   |                                                                                                                                                 
ggCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGtt                                                                                                                                                 <  2:1287838/150‑1 (MQ=255)
   ttCTGCCGACCAACCATCGGGCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTGCTGGCTGCAACCCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGAAGCGGCGTTGCt                                                                                                                                              <  2:77159/150‑1 (MQ=255)
   gtCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCt                                                                                                                                              <  2:620731/149‑1 (MQ=255)
     atGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTgg                                                                                                                                            <  2:1266740/149‑1 (MQ=255)
      tGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAACGCCGCATCGGCTTTGCTgtt                                                                                                                                           <  2:1256125/150‑3 (MQ=255)
       gCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATCCCCGCAGAGAGTTCTGGCTGCAAACCGCCTATTACAAACAGCGGAGGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGAt                                                                                                                                          >  1:521599/1‑150 (MQ=255)
       gCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGAt                                                                                                                                          >  1:641374/1‑150 (MQ=255)
       gCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGAt                                                                                                                                          >  1:1007763/1‑150 (MQ=255)
       gCCGACCAACCATCGCTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAACGCCGCATCGGCTTTGCTgttg                                                                                                                                          <  1:358215/150‑4 (MQ=255)
        ccGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAACGCCGCATCGGCTTTGCTgttgc                                                                                                                                         <  2:607127/150‑5 (MQ=255)
        ccGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATg                                                                                                                                         >  2:666687/1‑150 (MQ=255)
        ccGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTCGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATg                                                                                                                                         <  1:148533/150‑1 (MQ=255)
             caaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATg                                                                                                                                    >  1:1627469/1‑150 (MQ=255)
             caaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATg                                                                                                                                    >  1:912188/1‑150 (MQ=255)
              aaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGa                                                                                                                                   >  1:1516289/1‑150 (MQ=255)
              aaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGa                                                                                                                                   >  1:921109/1‑150 (MQ=255)
                ccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGAtt                                                                                                                                 <  2:1232439/150‑1 (MQ=255)
                ccaTCGGTCATGGACGAAGCCAATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGAATATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGAtt                                                                                                                                 <  1:205358/150‑1 (MQ=255)
                 caTCGGTCATGTACGAAGCCTATAAAGAGATTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGGAAACCGACTATTACAAACAGCTGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTa                                                                                                                                <  1:305232/150‑1 (MQ=255)
                 caTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTa                                                                                                                                <  1:1373253/150‑1 (MQ=255)
                 caTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTa                                                                                                                                <  1:831425/150‑1 (MQ=255)
                 caTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGGGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTa                                                                                                                                <  1:1072939/150‑1 (MQ=255)
                       tCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAacaaca                                                                                                                          >  2:1497844/1‑150 (MQ=255)
                          tGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATcc                                                                                                                       <  2:1443751/150‑1 (MQ=255)
                          tGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATcc                                                                                                                       >  2:1306442/1‑150 (MQ=255)
                                  gCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATc                                                                                                               <  2:1312457/150‑1 (MQ=255)
                                    cTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATccc                                                                                                             <  1:1380507/150‑1 (MQ=255)
                                     tataAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATcccc                                                                                                            >  2:291909/1‑150 (MQ=255)
                                        aaaGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGtt                                                                                                                   <  1:1126823/140‑1 (MQ=255)
                                        aaaGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGga                                                                                                         >  1:713913/1‑150 (MQ=255)
                                            aggttGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAGCCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGCTGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGCCt                                                                                                     >  2:1045128/4‑150 (MQ=255)
                                              tttGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACttt                                                                                                   >  2:719365/1‑150 (MQ=255)
                                                 gTTATGCAACTGGCCTCGTGGGATACGCGCTGAGATTTCTGGCTGCAAACCGCCTATTACTAACAGCGGCGGGTGGGCAAGAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTAcc                                                                                                <  1:586026/150‑1 (MQ=255)
                                                  ttATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACATACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTAccc                                                                                               >  1:281618/1‑150 (MQ=255)
                                                   tATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTCCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCcg                                                                                              <  2:1669689/150‑1 (MQ=255)
                                                    aTGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCcgc                                                                                             >  2:44562/1‑150 (MQ=255)
                                                        aaCTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCgg                                                                                         >  1:343243/1‑150 (MQ=255)
                                                        aaCTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCgg                                                                                         <  1:600827/150‑1 (MQ=255)
                                                            ggCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGTTGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCaa                                                                                     >  1:73681/1‑150 (MQ=255)
                                                              ccTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTTGGCAACAGCATAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATg                                                                                   <  1:876963/150‑1 (MQ=255)
                                                                tCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCTACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGAc                                                                                 <  2:687388/150‑1 (MQ=255)
                                                                  ttGGTATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATTGTTGGCAACAGCAAAGCCGATGCGGCGTTGATGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGTTCAATGACAg                                                                               <  1:823212/149‑1 (MQ=255)
                                                                    gggATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTTGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCg                                                                             <  2:1688277/150‑1 (MQ=255)
                                                                    gggATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCg                                                                             >  2:845616/1‑150 (MQ=255)
                                                                       aTACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCTGTCATTGACcgcgcgg                                                                          <  2:521599/150‑4 (MQ=255)
                                                                          cccGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGc                                                                       <  1:719365/150‑1 (MQ=255)
                                                                           ccGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTATCAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCt                                                                      >  2:1137364/1‑150 (MQ=255)
                                                                           ccGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTATCAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCt                                                                      <  1:62423/150‑1 (MQ=255)
                                                                             gCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTa                                                                    >  2:1434352/1‑150 (MQ=255)
                                                                             gCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTa                                                                    <  1:16408/150‑1 (MQ=255)
                                                                                gagagaTCTGGCTGCAAACCGACTATTACAAACAGCGGATCGTGGGCAACAGCAAAGCCGATGCGGCCTTGATGGATGAAATGATTTACAACATCCAGTTTATCCCCGGAGACTTTACCCCCGCGGTCAATGACAGCGTGAAGCTTAttt                                                                 >  1:1236021/1‑149 (MQ=255)
                                                                                gagagTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTg                                                                 <  2:1627469/150‑1 (MQ=255)
                                                                                gagagTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTg                                                                 <  2:709948/150‑1 (MQ=255)
                                                                                  gagTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGcc                                                               >  1:691950/1‑150 (MQ=255)
                                                                                  gagTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTATACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGcc                                                               >  2:306011/1‑150 (MQ=255)
                                                                                       ctgtccgCAACCCCACTATTACAAACCGCGGATCGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTCATCCCCGGAGACTTTACCCGCGCGCGCAATGTCAGCGTGAAGCTTATTGCCGAAAc                                                          <  1:672272/144‑1 (MQ=255)
                                                                                       cTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGTGGTCAATGACAGCGTGAAGCTTATTGCCGAAAc                                                          >  2:291396/1‑150 (MQ=255)
                                                                                       cTGGCTGCAAACCGACTATTACAAACAGCGGATGGTCCGCAACAGCAAAGCCGAAGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATACCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTCAAGCTTATAGCAGAAAc                                                          >  2:417612/1‑150 (MQ=255)
                                                                                        tGGCTGCAAACCGACTATTACAAACAGCGGATGTTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAAcc                                                         <  2:261794/150‑1 (MQ=255)
                                                                                           cTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACcgcg                                                      <  1:952542/150‑1 (MQ=255)
                                                                                           cTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACcgcg                                                      <  2:1554642/150‑1 (MQ=255)
                                                                                            tGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCgcgc                                                     >  1:780995/1‑150 (MQ=255)
                                                                                             gCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGcc                                                    <  2:743088/150‑1 (MQ=255)
                                                                                             gCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATAATTAACAACATCCAGTTTATCCCCGGAGACTTTCCCCGCGCGGTCAATGCCAGCGTGAAGCTTTTTGCCGAAACCGCGcc                                                    >  2:295260/1‑150 (MQ=255)
                                                                                               aaaCCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTg                                                  >  2:365574/1‑150 (MQ=255)
                                                                                                aaCCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGa                                                 >  1:1493615/1‑150 (MQ=255)
                                                                                                          tACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTAACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAAc                                       <  1:666687/150‑1 (MQ=255)
                                                                                                           aCAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGTGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAAcc                                      <  1:291396/150‑1 (MQ=255)
                                                                                                           aCAAACAGCGGATGGTGCGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACCACAACCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAAcc                                      <  1:62479/150‑1 (MQ=255)
                                                                                                             aaaCAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGATGCTAATAACCTg                                    <  1:199283/150‑1 (MQ=255)
                                                                                                              aaCAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGt                                   >  2:238394/1‑150 (MQ=255)
                                                                                                                 aGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTAc                                >  2:785224/1‑150 (MQ=255)
                                                                                                                  gCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACg                               <  2:424344/150‑1 (MQ=255)
                                                                                                                    ggATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTc                             <  1:365574/150‑1 (MQ=255)
                                                                                                                    ggATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTc                             <  2:337089/150‑1 (MQ=255)
                                                                                                                    ggATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTc                             <  2:1342358/150‑1 (MQ=255)
                                                                                                                    ggATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAAAATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTc                             <  2:1234038/150‑1 (MQ=255)
                                                                                                                     gATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATCATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGAAAGCGTGAAGCTTATTGCCGAAACCTCGCCTGACGCTAATAACCTGTTACGTCa                            >  1:302812/1‑150 (MQ=255)
                                                                                                                       tggtggGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGt                          >  2:1538146/1‑150 (MQ=255)
                                                                                                                         gtggGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCTGTAt                        >  1:495190/1‑150 (MQ=255)
                                                                                                                            ggCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGACACCGCGCCTCACGCTAATAACCTGTTACGTCAGTATGtt                     >  1:1074995/1‑150 (MQ=255)
                                                                                                                             gCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTg                    <  2:1493615/150‑1 (MQ=255)
                                                                                                                              cAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGc                   >  1:849675/1‑150 (MQ=255)
                                                                                                                              cAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGc                   <  2:1516289/150‑1 (MQ=255)
                                                                                                                              cAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGc                   <  2:1174200/150‑1 (MQ=255)
                                                                                                                                ccAGCAACGCCGCATCGGCTTTGCTGGATGAAATGATTAACACCATACAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCtt                 >  1:1195906/2‑150 (MQ=255)
                                                                                                                                 cAGCAACGCCGCATCGGCTTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCttt                >  2:1682611/1‑150 (MQ=255)
                                                                                                                                 cAGCAACGCCGCATCGGCTTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCttt                >  2:1342866/1‑150 (MQ=255)
                                                                                                                                  aGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCtttt               <  1:291909/150‑1 (MQ=255)
                                                                                                                                   gCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTg              >  2:773124/1‑150 (MQ=255)
                                                                                                                                   gCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTg              <  2:73681/150‑1 (MQ=255)
                                                                                                                                    cAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTgc             <  1:536945/150‑1 (MQ=255)
                                                                                                                                      aaGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTgcca           >  1:581361/1‑150 (MQ=255)
                                                                                                                                         ccGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTgccagcc        >  1:151726/1‑150 (MQ=255)
                                                                                                                                          cGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTgccagcca       <  1:1497844/150‑1 (MQ=255)
                                                                                                                                          cGATGCGGCGTTGCTGGATGAAATCATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCGGTTACGTCAGTATGTTGCTTTTgccagccc       >  2:1699031/1‑149 (MQ=255)
                                                                                                                                            aTGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTGCcagccagc     >  1:609865/1‑150 (MQ=255)
                                                                                                                                              gcggcgTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGt   >  2:1493419/1‑150 (MQ=255)
                                                                                                                                               cggcgTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGTg  >  2:1259579/1‑150 (MQ=255)
                                                                                                                                               cggcgTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGTg  >  2:337638/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCCAGTTTATCCCCGGAGACTTTACCCGCGCGGTCAATGACAGCGTGAAGCTTATTGCCGAAACCGCGCCTGACGCTAATAACCTGTTACGTCAGTATGTTGCTTTTGCCAGCCAGCGTG  >  NC_000913/3969294‑3969586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: