Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,971,996 T→A 10.8% L246Q (CTG→CAG)  wecC → UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,971,9960TA10.8% 316.2 / 20.8 120L246Q (CTG→CAG) wecCUDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (65/41);  new base A (6/7);  total (71/49)
Fisher's exact test for biased strand distribution p-value = 3.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

Only 100 of 121 total aligned reads displayed.

AAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCTTATCCGTACCGCGCGCGAAGTGAACGATCACAAAC  >  NC_000913/3971849‑3972145
                                                                                                                                                   |                                                                                                                                                     
aaGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCAgg                                                                                                                                                     <  2:781952/150‑1 (MQ=255)
aaGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATCTGTAAGCTCACCGAAAACAGCTTCCGCGATGTGAAAATCGCGTTTGCTAATGAATTGGCGCTGTTTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCATgg                                                                                                                                                     >  2:117656/1‑150 (MQ=255)
  ggTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCAGGCg                                                                                                                                                   <  2:1288208/150‑1 (MQ=255)
   gTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGa                                                                                                                                                  <  1:927273/150‑1 (MQ=255)
    tGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGaa                                                                                                                                                 >  1:521737/1‑150 (MQ=255)
      aGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATc                                                                                                                                               <  1:1178679/150‑1 (MQ=255)
       gtgtgtCGTCACTAACTCGCGTACGGCGGAAATGTGTAAGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCTGGGGATTAACGTCTGGGAACTGATTCGCCAGGCGAATca                                                                                                                                              <  1:831579/150‑2 (MQ=255)
       gtgtgtCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGGTAATGATTTGTCGCTGATTTGTGCCGATCAGGGTATTAACGTCTGGGAACTGATTCGCCTGGCGAATCg                                                                                                                                              >  1:1164135/1‑150 (MQ=255)
        tgtgtCGGCACTAACTGGCGGACGGGGGAAATGGGTAAGCTCACCGAAAACAGTTTCGGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGAGTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCAGGCGAATcag                                                                                                                                             <  2:297890/150‑3 (MQ=255)
           gtcgtcACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCGAAAACAGCTTCCGCGATGCGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCAGGCGAATcagttc                                                                                                                                          <  2:30927/150‑6 (MQ=255)
            tcgtcACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCAcc                                                                                                                                         <  2:65414/150‑1 (MQ=255)
             cgtcACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACCGATTCGCCTGGCGAATCGTCAccc                                                                                                                                        >  1:816297/1‑150 (MQ=255)
             cgtcACTAACTCGCGGACGGCGGAAATGAGTAAGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCAGGCGAATcagttcc                                                                                                                                         <  1:733360/149‑7 (MQ=255)
              gtcACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCt                                                                                                                                       >  1:1468276/1‑150 (MQ=255)
                   aaaCTCGCGGACGGCGGAAATGTGTAAGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGt                                                                                                                                  <  2:536812/149‑1 (MQ=255)
                    aaCTCGCGGACGGCGGAAATGTGTAAGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGtt                                                                                                                                 >  1:614474/1‑150 (MQ=255)
                         gctgacgacGGAAATGTGTAAGCTCACCGAAAACAGCTTCCGCAATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAatat                                                                                                                            <  1:1048887/147‑1 (MQ=255)
                           ggACTGCGGACATGTCTAAGCTCACCGAAAACAGCTTCCGCGATGTGACTATCGCTTTTGCTCATGAAGTGTCGCTGAGTTGTGCCGATCAGGGGAGTAAGGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGCTAATAttc                                                                                                                          <  1:930496/150‑1 (MQ=255)
                              cggcggAAATGTGTAAGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATAttcttc                                                                                                                       <  1:1459820/150‑1 (MQ=255)
                                   gAAATGTGTAAGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCATCAGcct                                                                                                                  >  1:599071/1‑150 (MQ=255)
                                     aaTGTGTAAGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGATTTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGcctgg                                                                                                                <  1:530764/150‑1 (MQ=255)
                                     aaTGTGTAAGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGcctgg                                                                                                                <  1:1224810/150‑1 (MQ=255)
                                        gtgtAAGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGcctggccc                                                                                                             >  1:616030/1‑150 (MQ=255)
                                        gtgtAAGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGcctggccc                                                                                                             >  1:1142470/1‑150 (MQ=255)
                                        gtgtAAGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGAGTTGTGCCGATCAGGGGATTTACGTCTGCGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGcctggccc                                                                                                             >  2:168641/1‑150 (MQ=255)
                                         tgtAAGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGcctggccct                                                                                                            >  1:1570604/1‑150 (MQ=255)
                                          gtAAGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGcctggccctg                                                                                                           <  1:1271283/150‑1 (MQ=255)
                                          gtAAGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGcctggccctg                                                                                                           >  1:334029/1‑150 (MQ=255)
                                          gtAAGCTCACAGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGATTTGTCGCTGATTTGTGCCGATCAGGGGATAAACGTCTGGGAACTGATTCGCCTGTCGAATCGTCACCCTCGCGTTAATATTCTTCAGcctggccctg                                                                                                           <  2:1111953/150‑1 (MQ=255)
                                             aGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCg                                                                                                        >  1:890955/1‑150 (MQ=255)
                                             aGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCg                                                                                                        <  2:773287/150‑1 (MQ=255)
                                               cTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGATCTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTg                                                                                                      <  1:697019/150‑1 (MQ=255)
                                               cTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTg                                                                                                      >  1:928692/1‑150 (MQ=255)
                                               cTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTg                                                                                                      >  1:967883/1‑150 (MQ=255)
                                                tCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTgg                                                                                                     <  1:645500/150‑1 (MQ=255)
                                                 cACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCCGCCTGGCCCTGGCGgggg                                                                                                    >  2:315721/1‑150 (MQ=255)
                                                  aCCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGc                                                                                                   >  1:1079556/1‑150 (MQ=255)
                                                   ccGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCg                                                                                                  <  1:1200872/150‑1 (MQ=255)
                                                    cGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCgg                                                                                                 <  2:161446/150‑1 (MQ=255)
                                                     gAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAAGATTCTTCAGCCTGGCCCTGGCGTGGGCGGt                                                                                                >  2:1210292/1‑150 (MQ=255)
                                                       aaaCAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGGCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCa                                                                                              <  1:33779/150‑1 (MQ=255)
                                                        aaCAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATTTTCTTCAGCCTGGCCCTGGCGTGGGCGGTCAc                                                                                             >  2:40511/1‑150 (MQ=255)
                                                        aaCAGCTTCCGCGATGTGAATATAGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGTATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCAc                                                                                             >  2:1050045/1‑150 (MQ=255)
                                                         aCAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACt                                                                                            <  2:45214/150‑1 (MQ=255)
                                                          cAGCTTCCGCGATGTGAATAACGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATAAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCATCAGCCTGGCCCTGGCGTGGGCGGTCACTg                                                                                           <  1:138669/150‑1 (MQ=255)
                                                            gCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCa                                                                                         >  2:548467/1‑150 (MQ=255)
                                                             cTTCCGCGATGGGAATATCGCTTTTGCTATTGATTTGTCGCTGCTTCGTGCCGATCAGGGGTTAAACGTCTGGGAACTGATTCGCCGGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCAt                                                                                        <  1:1260114/150‑1 (MQ=255)
                                                                      tgtgAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGa                                                                               >  2:536300/1‑150 (MQ=255)
                                                                      tgtgAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGGGCCGATCAGGGGATTAACGTCTGGGAACTGATTCTCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGa                                                                               >  2:619809/1‑150 (MQ=255)
                                                                        tgAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATc                                                                             >  1:135161/1‑150 (MQ=255)
                                                                        tgAATATCGCTTATGCTTATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGAGATTAGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGACACAGCATTGCTgatcata                                                                             >  1:12849/1‑144 (MQ=255)
                                                                         gAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGGTAAAATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATcc                                                                            >  2:445347/1‑150 (MQ=255)
                                                                              tCGCTATTGCTACTGAATTGGCCCTGATGAGTGCAGAGCAGGGGATTCACGGCTGGGAACTGATTCGCCTGGCGAATCGTCACCCGCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGt                                                                       <  1:1086934/150‑1 (MQ=255)
                                                                              gcGCTGTTGCTAATGAATTGGCGCGGATTTGTGCCGAGCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGGCACCCTCGCGGTAATATTCTTCAGCCTGGCCCTGGCGGGGGCGGGCACTGCATTGCTGTTGATCCGTGGt                                                                       <  1:83992/149‑1 (MQ=255)
                                                                               cGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGtt                                                                      >  2:851209/1‑150 (MQ=255)
                                                                                gCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGttt                                                                     >  2:486566/1‑150 (MQ=255)
                                                                                   tttGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATc                                                                  <  1:744327/150‑1 (MQ=255)
                                                                                    ttGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCg                                                                 <  2:795454/150‑1 (MQ=255)
                                                                                      gCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTg                                                               >  2:768528/1‑150 (MQ=255)
                                                                                       cTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTgg                                                              <  2:1355782/150‑1 (MQ=255)
                                                                                       cTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTgg                                                              >  2:1630100/1‑150 (MQ=255)
                                                                                       cTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTATGGGAACTGAATCGACTGGCGAATCGTCACCCTAGCGTTAATATTCTTCCGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTCTTGATCCGTGGATTATCGTgg                                                              >  2:507797/1‑150 (MQ=255)
                                                                                        tAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGc                                                             >  1:1125503/1‑150 (MQ=255)
                                                                                         taTGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGcc                                                            >  1:80231/2‑149 (MQ=255)
                                                                                         aaTGACTTGTCGCTGATTTGGGCCGATCAGGGGAGTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGca                                                            <  2:734738/150‑1 (MQ=255)
                                                                                         aaTGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGca                                                            >  1:364576/1‑150 (MQ=255)
                                                                                          aTGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGcac                                                           <  2:1158591/150‑1 (MQ=255)
                                                                                          aTGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGcac                                                           <  2:521737/150‑1 (MQ=255)
                                                                                           tGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGcaca                                                          >  2:182043/1‑150 (MQ=255)
                                                                                            gAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGACTCGTCACCCTCGCGTTACTATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAg                                                         >  2:1095564/1‑150 (MQ=255)
                                                                                             aaTTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGa                                                        >  1:1698564/1‑150 (MQ=255)
                                                                                                 gTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAAccc                                                    >  1:51918/1‑150 (MQ=255)
                                                                                                   cGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTTCTCCGTGGTTTATCGTGGCACAGAAccccc                                                  >  1:1507628/1‑150 (MQ=255)
                                                                                                   cGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAAccccc                                                  <  2:623204/150‑1 (MQ=255)
                                                                                                       gATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCcagca                                              <  1:1320664/150‑1 (MQ=255)
                                                                                                             tgCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCAATGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGcgcg                                        >  2:1391349/1‑150 (MQ=255)
                                                                                                               ccGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGc                                      <  2:364576/150‑1 (MQ=255)
                                                                                                               ccGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCAGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCACCAGGCGCGGc                                      >  1:735535/1‑150 (MQ=255)
                                                                                                               ccGATCAGGGGATTAACGTCTGGGAACTGATTAGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGc                                      >  1:1169119/1‑150 (MQ=255)
                                                                                                                cGATCAGGGGATTAACGTCTGGGAACTGATTCGCATGTCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCt                                     <  2:912998/150‑1 (MQ=255)
                                                                                                                 gATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCtt                                    >  1:423695/1‑150 (MQ=255)
                                                                                                                 gATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCtt                                    >  2:657465/1‑150 (MQ=255)
                                                                                                                    cAGGGGATTAACGGCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCTTATc                                 <  1:876056/150‑1 (MQ=255)
                                                                                                                       gggATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGCTCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCTTATCCGt                              >  2:1221609/1‑150 (MQ=255)
                                                                                                                             aaCGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCTTATCCGTACcgcg                        >  1:335448/1‑150 (MQ=255)
                                                                                                                              aCGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCTTATCCGTACcgcgc                       >  2:814371/1‑150 (MQ=255)
                                                                                                                                ctCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCTTATCCGTACcgcgcgc                     >  1:1712225/2‑150 (MQ=255)
                                                                                                                                     ggtgacgATTCGCCAGGCGAATCGTCCCCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGCAGCCCCAGCAGGCGCGGCGTATCCGTCCCGCGCGCGCAGt                >  2:668616/7‑150 (MQ=255)
                                                                                                                                      gtgacgATTCGCCAGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCCCTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCTTATCCGTACCGCGCGCGAAGTg               >  2:119453/6‑150 (MQ=255)
                                                                                                                                      gtgacgATTCGCCAGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCTTATCCGTACCGCGCGCGAAGTg               >  1:600110/6‑150 (MQ=255)
                                                                                                                                       tgacgATTCGACAGGCGAATCATCACACTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATGCGTGGTTTATCGTGGCACCGCACCCCCAGCAGGCGCGGCTTATCCGTACGGCGCGCGAAGTgg              >  1:193180/5‑149 (MQ=255)
                                                                                                                                        gacgATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCTTATCCGTACCGCGCGCGAAGTGaa             >  1:184200/4‑150 (MQ=255)
                                                                                                                                         cTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCTTATCCGTACCGCGCGCGAAGTGAAc            >  2:359079/1‑150 (MQ=255)
                                                                                                                                              tCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCTTATCCGTACCGCGCGCGAAGTGAACGATca       <  2:1081081/150‑1 (MQ=255)
                                                                                                                                               cGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCCGGCGCGGCTTATCCGTACCGCGCGCGAAGTGAACGATcac      >  1:855648/1‑150 (MQ=255)
                                                                                                                                               cGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCTTATCCGTACCGCGCGCGAAGTGAACGATcac      <  1:548467/150‑1 (MQ=255)
                                                                                                                                               cGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCTTATCCGTACCGAGCGCGAAGTGAACGATcac      >  2:152698/1‑150 (MQ=255)
                                                                                                                                                gCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCTTATCCGTACCGCGCGCGAAGTGAACGATcaca     >  1:1658247/1‑150 (MQ=255)
                                                                                                                                                gCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCTTATCCGTACCGCGCGCGAAGTGAACGATcaca     <  2:890955/150‑1 (MQ=255)
                                                                                                                                                   tGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCTTATCCGTACCGCGCGCGAAGTGAACGATCACAAAc  >  2:122049/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAATGAATTGTCGCTGATTTGTGCCGATCAGGGGATTAACGTCTGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGCCCTGGCGTGGGCGGTCACTGCATTGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCCCAGCAGGCGCGGCTTATCCGTACCGCGCGCGAAGTGAACGATCACAAAC  >  NC_000913/3971849‑3972145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: