Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 501,469 G→T 5.2% intergenic (+40/+93) gsk → / ← ybaL inosine/guanosine kinase/inner membrane putative NAD(P)‑binding transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913501,4690GT 5.2% 346.0 / 5.1 113intergenic (+40/+93)gsk/ybaLinosine/guanosine kinase/inner membrane putative NAD(P)‑binding transporter
Reads supporting (aligned to +/- strand):  ref base G (52/55);  new base T (3/3);  total (55/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 118 total aligned reads displayed.

GCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTA  >  NC_000913/501325‑501616
                                                                                                                                                |                                                                                                                                                   
gcaaaCCGTGTGAGCTAACAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTAACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGCTAAGGGGTTCACGCCGCAtgg                                                                                                                                                >  2:298082/4‑148 (MQ=255)
gCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTCAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCt                                                                                                                                                >  2:1581339/1‑150 (MQ=255)
 cTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACATCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACAGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGTCGTTCACGCCGCATCTg                                                                                                                                               >  2:1401646/1‑150 (MQ=255)
  tcaCCGTGTGAGCTATCAGGTACTGAACCACCCTTCACCTCGTTTACCGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTGCGTAGGCCAGATAAGGCGTTCACGCCGCATCTgg                                                                                                                                              <  1:861698/148‑1 (MQ=255)
    aCCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTACCGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCa                                                                                                                                            <  1:526558/150‑1 (MQ=255)
    aCCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATGCGGCGTGAACGCCTTATCTGGcc                                                                                                                                            <  1:716966/150‑2 (MQ=255)
        tgtgAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTg                                                                                                                                        <  2:1250322/150‑1 (MQ=255)
         gtgAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATGCGGCGTGAACGCCTTATCTGGcctacga                                                                                                                                       <  1:1557786/150‑7 (MQ=38)
         gtgAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTgg                                                                                                                                       <  2:1559017/150‑1 (MQ=255)
          tgAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGc                                                                                                                                      <  1:308663/150‑1 (MQ=255)
          tgAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGc                                                                                                                                      <  2:1606401/150‑1 (MQ=255)
           gAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTACCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGct                                                                                                                                     >  2:663058/1‑150 (MQ=255)
             gCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGctct                                                                                                                                   >  2:479139/1‑150 (MQ=255)
             gCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGctct                                                                                                                                   <  2:1666016/150‑1 (MQ=255)
              cTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCtctc                                                                                                                                  <  2:926239/150‑1 (MQ=255)
                    ggTACTGACCCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGACGAGGCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGcc                                                                                                                            <  1:1090106/150‑1 (MQ=255)
                    ggTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGcc                                                                                                                            <  1:817160/150‑1 (MQ=255)
                    ggTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGcc                                                                                                                            <  1:35549/150‑1 (MQ=255)
                    ggTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGcc                                                                                                                            <  2:375735/150‑1 (MQ=255)
                    ggTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGcc                                                                                                                            >  2:909404/1‑150 (MQ=255)
                    ggTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGGCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGcc                                                                                                                            <  1:41153/150‑1 (MQ=255)
                     gTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTAACGCCGCATCTGGCATTTGGCTCTCGATGCCt                                                                                                                           >  2:395602/1‑150 (MQ=255)
                      tACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTg                                                                                                                          <  2:108567/150‑1 (MQ=255)
                      tACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTg                                                                                                                          <  1:900664/150‑1 (MQ=255)
                      tACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTg                                                                                                                          <  1:1303366/150‑1 (MQ=255)
                             ccAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTGAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGAc                                                                                                                   >  1:160818/1‑150 (MQ=255)
                               aGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGc                                                                                                                 >  1:1430204/1‑150 (MQ=255)
                                 cATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTg                                                                                                               <  1:391151/150‑1 (MQ=255)
                                  aTTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGTCAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTgg                                                                                                              >  1:642084/1‑150 (MQ=255)
                                      cccTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGcgcg                                                                                                          <  1:1385808/149‑1 (MQ=255)
                                      aCCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGcgcg                                                                                                          <  2:1157194/150‑1 (MQ=255)
                                        cTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTc                                                                                                        <  2:886515/150‑1 (MQ=255)
                                         tCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGAGCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCt                                                                                                       <  2:1188819/150‑1 (MQ=255)
                                          cGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCtt                                                                                                      >  2:200713/1‑150 (MQ=255)
                                          cGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCtt                                                                                                      >  2:1235901/1‑150 (MQ=255)
                                           gTTTAACGCGCGGCTTACCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTa                                                                                                     <  1:663058/150‑1 (MQ=255)
                                            tttAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                    <  2:307072/150‑1 (MQ=255)
                                             ttAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATc                                                                                                   >  1:1356243/1‑150 (MQ=255)
                                              tcaCGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCa                                                                                                  >  2:949366/3‑150 (MQ=255)
                                              tAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGCTGCCTGATGCGACGCTGGCGCGTCTTATCa                                                                                                  >  1:828786/1‑150 (MQ=255)
                                                aCGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATg                                                                                                >  2:1047314/1‑150 (MQ=255)
                                                  gcgcgGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGcc                                                                                              >  2:1020485/1‑150 (MQ=255)
                                                   cgcgGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGccg                                                                                             >  1:553851/1‑149 (MQ=255)
                                                   cgcgGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCt                                                                                             >  1:546909/1‑150 (MQ=255)
                                                   cgcgGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCt                                                                                             <  2:1051473/150‑1 (MQ=255)
                                                       gCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACat                                                                                         >  1:397794/1‑150 (MQ=255)
                                                       gCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACat                                                                                         >  1:1322390/1‑150 (MQ=255)
                                                             cGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATAttttt                                                                                   <  1:909404/150‑1 (MQ=255)
                                                              ggAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTc                                                                                  >  2:582384/1‑150 (MQ=255)
                                                                  cGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCatat                                                                              >  1:409644/1‑150 (MQ=255)
                                                                     taaGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATAtttt                                                                           <  2:1701365/149‑1 (MQ=255)
                                                                     gAAGACAGCCTGGTAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATAtttt                                                                           <  1:33385/150‑1 (MQ=255)
                                                                      aaGACAGCCTGGACGAGTCTAACTGGGATCTTTAAGTTATCGTCCGATCGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTATCATATTTTa                                                                          <  1:1136883/150‑1 (MQ=37)
                                                                       aGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTAc                                                                         <  1:566850/150‑1 (MQ=255)
                                                                        gACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACa                                                                        >  1:1719890/1‑150 (MQ=255)
                                                                            gCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCg                                                                    >  1:1435386/1‑150 (MQ=255)
                                                                              cTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGc                                                                  <  1:1639714/150‑1 (MQ=255)
                                                                                 gAAGAGGCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAac                                                               <  2:861539/150‑1 (MQ=255)
                                                                                       tCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGt                                                         >  1:314122/1‑150 (MQ=255)
                                                                                        cTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGtt                                                        >  1:1083619/1‑150 (MQ=255)
                                                                                        cTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGtt                                                        >  1:504370/1‑150 (MQ=255)
                                                                                           aCTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAc                                                     >  1:141175/1‑150 (MQ=255)
                                                                                            cTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAct                                                    <  1:949366/150‑2 (MQ=255)
                                                                                             tGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAccc                                                   <  2:1324015/150‑1 (MQ=255)
                                                                                             tGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAccc                                                   <  2:1356243/150‑1 (MQ=255)
                                                                                             tGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAccc                                                   <  2:1552245/150‑1 (MQ=255)
                                                                                              gggATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAcccc                                                  >  2:1377614/1‑150 (MQ=255)
                                                                                               ggATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCg                                                 >  2:251625/1‑150 (MQ=255)
                                                                                               ggATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCg                                                 >  1:950879/1‑150 (MQ=255)
                                                                                               ggATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCAAATTTTACATCCGGCAACCACCGTTTACCCCg                                                 >  1:957029/1‑150 (MQ=255)
                                                                                                 aTCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTc                                               <  1:1047314/150‑1 (MQ=255)
                                                                                                 aTCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTc                                               <  1:1581018/150‑1 (MQ=255)
                                                                                                   cGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCAAGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTcac                                             >  2:1319158/1‑150 (MQ=255)
                                                                                                    gTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTcacc                                            >  2:721816/1‑150 (MQ=255)
                                                                                                    gTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTcacc                                            >  1:438164/1‑150 (MQ=255)
                                                                                                    gTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCAATTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTcacc                                            >  1:473522/1‑150 (MQ=255)
                                                                                                         gTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTc                                       >  2:1165520/1‑150 (MQ=255)
                                                                                                               gtcgGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACccgc                                 <  2:653033/150‑1 (MQ=255)
                                                                                                               gtcgGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCAGGCAACCACCGTTTACCCCGTCACCACCTCACccgc                                 >  2:1210952/1‑150 (MQ=255)
                                                                                                                tcgGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACccgcc                                >  1:122804/1‑150 (MQ=255)
                                                                                                                  ggTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGACTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCgg                              >  2:1187616/1‑150 (MQ=255)
                                                                                                                        aatgCCAGATGCGGCGTGAACGCCTTATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGt                        >  2:918107/4‑150 (MQ=38)
                                                                                                                         aGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGtt                       >  2:224527/1‑150 (MQ=255)
                                                                                                                           gCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTc                     <  2:1585574/150‑1 (MQ=255)
                                                                                                                            ccAGATGCGGCGTGAACGCCTTATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTcc                    >  2:623413/1‑150 (MQ=39)
                                                                                                                            ccAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTcc                    >  2:1601422/1‑150 (MQ=255)
                                                                                                                             cAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCca                   <  1:1119415/150‑1 (MQ=255)
                                                                                                                              agatgcggCGTGAACGCCTTATATGGCATTTGGCTCTCGCTGACTGATGCGACGCTGGCGCGTAATATCATGACTACATATTTTTCATATTTTACATCAGGAACCCACCGTTTACCCCGTCACCACCTCGCACGCCGGTGGCGTTTCaag                  >  2:137314/7‑150 (MQ=255)
                                                                                                                              aGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCcag                  <  1:167555/150‑1 (MQ=255)
                                                                                                                               gATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCcagc                 <  1:1581339/150‑1 (MQ=255)
                                                                                                                                  aaGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGt              >  2:1011736/1‑150 (MQ=255)
                                                                                                                                   aGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGtt             <  1:1520668/150‑1 (MQ=255)
                                                                                                                                     gCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTcc           <  2:1651834/150‑1 (MQ=255)
                                                                                                                                      cGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTcc                    >  1:1132199/1‑140 (MQ=255)
                                                                                                                                       gTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAg         >  2:1533162/1‑150 (MQ=255)
                                                                                                                                         tCACGCCGCATCTGGCATTTGGCTCTCGATGCCAGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCa       <  1:1485499/150‑1 (MQ=255)
                                                                                                                                          ctcgccgcATCTGGCATTTTGCTCTCGATGCCTGAAGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGATTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCAt      <  2:496770/148‑1 (MQ=255)
                                                                                                                                            cgccgcATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATgg    >  1:1359755/1‑150 (MQ=255)
                                                                                                                                             gccgcATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAACCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCAAGCATGGt   >  1:685129/1‑150 (MQ=255)
                                                                                                                                              ccgcATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTa  <  2:1216250/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTA  >  NC_000913/501325‑501616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: