Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,972,674 G→T 6.5% E51D (GAG→GAT) ‡ rffG → dTDP‑glucose 4,6‑dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,972,6740GT 6.5% 371.8 / 11.4 131E51D (GAG→GAT) ‡rffGdTDP‑glucose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base G (58/62);  new base T (6/3);  total (66/65)
Fisher's exact test for biased strand distribution p-value = 3.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01

Only 100 of 136 total aligned reads displayed.

GAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACC  >  NC_000913/3972526‑3972821
                                                                                                                                                    |                                                                                                                                                   
gaAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAagcgagc                                                                                                                                                    >  2:658238/1‑150 (MQ=255)
gaAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAagcgagc                                                                                                                                                    >  1:188598/1‑150 (MQ=255)
   aaaTTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCt                                                                                                                                                 <  1:787178/150‑1 (MQ=255)
     aTTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCttt                                                                                                                                               >  1:998686/1‑150 (MQ=255)
      ttCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTg                                                                                                                                              <  2:982857/150‑1 (MQ=255)
       tCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGc                                                                                                                                             <  2:1569359/150‑1 (MQ=255)
       tCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGc                                                                                                                                             <  1:125151/150‑1 (MQ=255)
        cTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGCCGCGGTGGTAGTGGTCGTTAAGCTGACCTCCGCCGGAAACCTGATGTCGGTGGCACCGGTCGCGCCAAGCGCGCGGTTTGcc                                                                                                                                            >  1:1661368/1‑150 (MQ=255)
        cTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGcc                                                                                                                                            <  1:115454/150‑1 (MQ=255)
        cTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAACCAGCCCTTTGcc                                                                                                                                            >  1:1426059/1‑150 (MQ=255)
         tGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGCTCGCTTTGcgc                                                                                                                                           <  2:1361274/150‑3 (MQ=255)
           aTAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGAGGGGGTGGTAGTGGTCGATAAGCTGACCTAAGCCGGAAGCCTGATGTCGCTGGCTCCGGTCGCGCAAAGAGAGAGCTTTGCCttt                                                                                                                                         >  1:928007/1‑150 (MQ=255)
            tAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGCTCGCTTTGcgcgac                                                                                                                                        <  1:1574652/150‑6 (MQ=255)
            tAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTg                                                                                                                                        <  2:705861/150‑1 (MQ=255)
             accAGGTGGTGCCGGGTGTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAACCGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTga                                                                                                                                       <  1:1647657/148‑1 (MQ=255)
             aaCAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTga                                                                                                                                       >  1:1173204/1‑150 (MQ=255)
                aGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAACGACCGCATTGACTTTGAGaa                                                                                                                                    >  2:1677130/1‑150 (MQ=255)
                 ggtggtGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGaaa                                                                                                                                   <  2:1684340/150‑1 (MQ=255)
                   tggtgCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCTAAGCGAGCGCGTTGCCTTTGAGAAAGt                                                                                                                                 >  1:1548407/1‑150 (MQ=255)
                   tggtgCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGt                                                                                                                                 >  1:65889/1‑150 (MQ=255)
                    ggtgCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGCGCGACGCGGGGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGGCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGtt                                                                                                                                <  2:646786/150‑1 (MQ=255)
                    ggtgCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGtt                                                                                                                                >  2:1181199/1‑150 (MQ=255)
                     gtgCCGGGTTTATTGGCTCGGCGCTTGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTg                                                                                                                               <  1:1484828/150‑1 (MQ=255)
                     gtgCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTg                                                                                                                               <  1:1491550/150‑1 (MQ=255)
                        ccGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGata                                                                                                                            <  1:539382/150‑1 (MQ=255)
                             tttATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAACCGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGGCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGc                                                                                                                       <  2:1096550/150‑1 (MQ=255)
                             tttATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTAAGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGc                                                                                                                       <  2:1322546/150‑1 (MQ=255)
                                 ttGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATc                                                                                                                   <  1:189583/150‑1 (MQ=255)
                                   ggCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCCAAGCGAGCCCGTTGCCTTTGAGAAAGTTGATATCTGCGATCgg                                                                                                                 >  1:1404386/1‑150 (MQ=255)
                                     cTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGc                                                                                                               >  1:91672/1‑150 (MQ=255)
                                      tCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTTGCGTTGAGAAAGTTGATATATGCGATCGGGcc                                                                                                              >  2:1561779/1‑149 (MQ=255)
                                       cGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAg                                                                                                             <  1:304568/150‑1 (MQ=255)
                                        ggCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCCAAGCGGGCGGGTTGGCGGTGTGAAAGTTTTTTTATGCGATCGGGccgc                                                                                                            >  1:1304406/1‑147 (MQ=255)
                                        agcgcTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGGCGATAAGCTGACCTACTCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGa                                                                                                            <  2:488514/149‑1 (MQ=255)
                                         gcgcTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGaa                                                                                                           <  2:1024188/150‑1 (MQ=255)
                                         gcgcTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCCACCGCTATGCCTTAGAGAAAGTTGTTAACTGCCCTAGGGGAGaa                                                                                                           >  1:374797/1‑150 (MQ=255)
                                            cTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGCACTg                                                                                                        >  2:1107530/1‑150 (MQ=255)
                                              ggTGCGTGATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGc                                                                                                      <  2:12225/150‑1 (MQ=255)
                                                tGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCAc                                                                                                    <  1:1124663/150‑1 (MQ=255)
                                                  cGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGACACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCAcgc                                                                                                  >  2:1584383/1‑150 (MQ=255)
                                                   gTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCAcgcg                                                                                                 <  1:1170137/150‑1 (MQ=255)
                                                        ttcatcaACGAAACGCGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTc                                                                                            <  1:298247/149‑1 (MQ=255)
                                                        atcatcTAAGAAACGAGCGACGCGGCGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTc                                                                                            <  2:174513/150‑1 (MQ=255)
                                                           atcaACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGCACTGGCACGCGTATTCACt                                                                                         >  1:323591/1‑150 (MQ=255)
                                                           atcaACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACt                                                                                         <  1:1369465/150‑1 (MQ=255)
                                                           atcaACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACt                                                                                         >  1:1146828/1‑150 (MQ=255)
                                                           atcaACGAAACGAGCGACGCGGTGGTAGTGGGCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACt                                                                                         <  2:593757/150‑1 (MQ=255)
                                                            tcaACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTg                                                                                        <  2:33170/150‑1 (MQ=255)
                                                               acgaaacgaGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGc                                                                                     <  2:552030/150‑1 (MQ=255)
                                                                 gaaacgaGCGACGCGGTGGTAGTGGGCGATAAGCTGACCTGCTCCGGAAACCTGATGACGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCAt                                                                                   <  2:852818/150‑1 (MQ=255)
                                                                  aaacgaGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCCTc                                                                                  >  2:291029/1‑150 (MQ=255)
                                                                  aaacgaGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATc                                                                                  <  1:1715556/150‑1 (MQ=255)
                                                                   aacgaGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCa                                                                                 >  1:1023159/1‑150 (MQ=255)
                                                                     cgaGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATGTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGc                                                                               >  1:241640/1‑150 (MQ=255)
                                                                       aGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCa                                                                             >  2:862546/1‑150 (MQ=255)
                                                                        gCGACGCGGTGGTAGTGGACGATAAGCTGACCTAAGCCGGAAACCTGATGTCGCTGGCTCCGGTCGCGCAAAGCGAGCGCTTAGCCTTTGAGAAAGTTGATCTCTGCGGTCGGGCAGAACTGGCACGCGTATTAAGAGAGCATCAGcgat                                                                            >  2:267109/1‑147 (MQ=255)
                                                                          gACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGAc                                                                          >  2:392408/1‑150 (MQ=255)
                                                                            cgcgGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACtg                                                                        <  2:1331066/150‑1 (MQ=255)
                                                                              cgGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCTGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACTCGTATTCACTGAGCATCAGCGAGACtgtg                                                                      <  1:829933/150‑1 (MQ=255)
                                                                                 tggtAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCa                                                                   <  2:998686/150‑1 (MQ=255)
                                                                                    tAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGCGAAAGTTGATATCTGCGATCGGGCAGCACTGGCACGCGTATTCAGTGAGCATCAGCCAGCCTGTGTCCTGc                                                                >  1:48605/1‑150 (MQ=255)
                                                                                    tAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATATGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGc                                                                >  1:862607/1‑150 (MQ=255)
                                                                                        ggTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCt                                                            >  2:259788/1‑150 (MQ=255)
                                                                                        ggTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATATGCGATAGGGCAGAACTCGCACGCGTATTCACTGAGCATCAGCCAGCAGGTGTAATGCAACt                                                            >  2:434277/1‑150 (MQ=255)
                                                                                        ggTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTGTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCt                                                            >  1:268426/1‑150 (MQ=255)
                                                                                         gTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTg                                                           >  2:1495276/1‑150 (MQ=255)
                                                                                           cGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCCCGCGTATTCCATGAGCCTCAGCCCGACTGTGTCATGCAAATGGc                                                         >  2:1546828/1‑150 (MQ=255)
                                                                                           cGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGAATCAGCCAGACTGTGTCATGCTTCTGGc                                                         <  2:188598/150‑1 (MQ=255)
                                                                                                 gCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCCGCCGa                                                   >  2:269391/1‑150 (MQ=255)
                                                                                                    gACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAg                                                <  1:445637/150‑1 (MQ=255)
                                                                                                     aCCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGc                                               <  2:206700/150‑1 (MQ=255)
                                                                                                        tACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCAt                                            <  2:1269760/150‑1 (MQ=255)
                                                                                                        tACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCAt                                            <  2:1698785/150‑1 (MQ=255)
                                                                                                         aCGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCCGCCGAAAGCCATg                                           >  2:334784/1‑150 (MQ=255)
                                                                                                         aCGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATg                                           >  2:1180387/1‑150 (MQ=255)
                                                                                                         aCGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATg                                           <  1:1005904/150‑1 (MQ=255)
                                                                                                            ccGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTg                                        <  2:807937/150‑1 (MQ=255)
                                                                                                              gttaaCCTGATGTCGCTGGCAGCGGTCGCGCAATGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAAGTTGCACGCGTATTCACTGAGCATCAGCGAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGAc                                      <  2:191688/147‑1 (MQ=255)
                                                                                                               gAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGAcc                                     >  2:216/1‑150 (MQ=255)
                                                                                                                   ccTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTc                                 <  2:1016017/150‑1 (MQ=255)
                                                                                                                       aTGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCCGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTAtt                             >  1:1010115/1‑150 (MQ=255)
                                                                                                                         gTCGCTGGCACCGGTCGCGCAAAGCGCGCGGGTTGCCGTTGAGAAAGTTGATATATGCGATCGGGCAGAAATGGCCCCCGTATTCACTGAGCCTCAGCCAGACTGTGTACTGCATCTTGGAGCCGAAAGCCATGTTGACCGTTCTATTGa                           >  2:425740/1‑150 (MQ=255)
                                                                                                                          tCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGAc                          <  1:392408/150‑1 (MQ=255)
                                                                                                                           cGCTGGCACCGGTCGCGCAAAGCGCGCGGGTTGCCAGTGAGAAAGTTGATATCTGCGATCGGGCAGAACAGGCACGCGGATTAACATCGCCTAAGCCAGCCAGTGGCATGCAACTGGCAGCCCAAACCCCTGTTGACCCGTCTATTGAcc                         >  2:871947/1‑149 (MQ=255)
                                                                                                                           cGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACg                         <  2:223589/150‑1 (MQ=255)
                                                                                                                                     ggTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGc               >  2:561478/1‑150 (MQ=255)
                                                                                                                                     ggTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGGTGATATCTGCGATCGGGCAGAACAGGCACGCGTATTGACTGAGCATCAGCCAGCCACTGACAAGCATCTGTCAGACCAACTCCATATTGAACGTTCTATTCACGGCCCCGAAGc               >  2:873524/1‑150 (MQ=255)
                                                                                                                                      gTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCg              >  1:1536376/1‑150 (MQ=255)
                                                                                                                                      gTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACAGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCg              <  2:746425/150‑1 (MQ=255)
                                                                                                                                       aaaggcAAAGCGCTCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGt             >  1:728277/5‑150 (MQ=255)
                                                                                                                                        cgcgCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGtt            >  2:841600/1‑150 (MQ=255)
                                                                                                                                        aaggcAAAGCGCTCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCTTCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGtt            >  2:1579036/4‑150 (MQ=255)
                                                                                                                                         gcgcAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGttt           <  2:1404386/150‑1 (MQ=255)
                                                                                                                                         gcgcAAAGCGAGCGCTTTGCCTTTGAGAAAGGTGAGATCGGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCAGCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGttt           <  1:291029/150‑1 (MQ=255)
                                                                                                                                         aggcAAAGCGCTCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGttt           >  1:491257/3‑150 (MQ=255)
                                                                                                                                           gcAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTAt         >  1:27041/1‑150 (MQ=255)
                                                                                                                                           gcAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTAt         <  2:928007/150‑1 (MQ=255)
                                                                                                                                              aaGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGa      >  1:1477379/1‑150 (MQ=255)
                                                                                                                                                 cgagcgCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAAc   <  2:27041/150‑1 (MQ=255)
                                                                                                                                                  gagcgCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAAcc  <  1:1495276/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAAAAATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGACGCGGTGGTAGTGGTCGATAAGCTGACCTACGCCGGAAACCTGATGTCGCTGGCACCGGTCGCGCAAAGCGAGCGCTTTGCCTTTGAGAAAGTTGATATCTGCGATCGGGCAGAACTGGCACGCGTATTCACTGAGCATCAGCCAGACTGTGTCATGCATCTGGCAGCCGAAAGCCATGTTGACCGTTCTATTGACGGCCCGGCAGCGTTTATTGAAACC  >  NC_000913/3972526‑3972821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: