Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,989,188 A→T 5.8% V214D (GTC→GAC)  hemD ← uroporphyrinogen III synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,989,1880AT 5.8% 299.2 / 6.5 104V214D (GTC→GAC) hemDuroporphyrinogen III synthase
Reads supporting (aligned to +/- strand):  ref base A (47/50);  new base T (3/3);  total (51/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

Only 100 of 108 total aligned reads displayed.

GGAGGTTTTTTCTTGTTCCGTCATTATGGCTTCCTGTTATGAGAGTTATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCTCGCGGGCTTGCCAGCGCATCGCTTCTTCTGCACCATCGTA  >  NC_000913/3989043‑3989336
                                                                                                                                                 |                                                                                                                                                    
ggAGGTTTTTTCTTGTTCCGTCATTATGGCTTCCTGTTATGAGAGTTATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTgacgac                                                                                                                                                  <  2:1374430/150‑1 (MQ=255)
 gAGGTTTTTTCTTGTTCCGTCATTATGGCTTCCTGTTATGAGAGTTATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGATGAcc                                                                                                                                                 <  2:683367/150‑1 (MQ=255)
    gTTTTTTCTTGTTCCGTCATTATGGCTTCCTGTTATGAGAGTTATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAAt                                                                                                                                              >  1:1330668/1‑150 (MQ=255)
     ttttttCTTGTTCCGTCATTATGGCTTCCTGTTATGAGAGTTATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATa                                                                                                                                             >  1:993860/1‑150 (MQ=255)
      tttttCTTGTTCCGTCATTATGGCTTCCTGTTATGAGAGTTATTGTAATGTCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAg                                                                                                                                            <  2:766996/150‑1 (MQ=255)
       ttttCTTGTTCCGTCATTATGGCTTCCTGTTATGAGAGTTATTGTAATGCCCGTAAAAGCGCATCGTTGGCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGt                                                                                                                                           <  2:920202/150‑1 (MQ=255)
        tttCTTGTTCCGTCATTATGGCTTCCTGTTATGAGAGTTATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTc                                                                                                                                          <  2:1343817/150‑1 (MQ=255)
         ttCTTGTTCCGTCATTATGGCTTCCTGTTATGAGAGTTATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGTCCAATAGTCg                                                                                                                                         <  1:215546/150‑1 (MQ=255)
           cTTGTTCCGTCATTATGGCTTCCTGTTATGAGAGTTATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGAc                                                                                                                                       <  1:812541/150‑1 (MQ=255)
           cTTGTTCCGTCATTATGGCTTCCTGTTATGAGAGTTATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGAc                                                                                                                                       <  1:1155006/150‑1 (MQ=255)
           cTTGTTCCGTCATTATGGCTTCCTGTTATGAGAGTTATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGAc                                                                                                                                       >  1:1049101/1‑150 (MQ=255)
                tCCGTCATTCTGGCTGCCTCTTCTGCGAGTAATTGTAATGCCCTTAATAGCGCATCGTTGTCAGCGGTATCGGCGACCTTAATGTCTTGGCTGCAAAGTTCCCGGGCGAGTTTCGCGAAACGCTCACTGACGACCAATAGTCGACAgtgt                                                                                                                                  <  2:468357/150‑1 (MQ=255)
                tCCGTCATTATGTCTTCCTGTTATGAGAGTTATTGTACTGCCCGTAAAAGCGCATCGTTGTCAGCGTTTTCGGCGACCTTAATGTCTTGCCATCACTTTTCCAGGGCGAGTTTCGCCAAACGATCACTGACGACCAATAGTAGACAgtgt                                                                                                                                  <  2:1227190/150‑1 (MQ=255)
                tCCGTCATTATGGCTTCCTGTTATGAGAGTTATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGTCGACTATTGGTCGTCAgtga                                                                                                                                  <  2:332577/150‑2 (MQ=255)
                 ccGTCATTATGGCTTCCTGTTATGAGAGTTATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGTCGACTATTGGTCGTCAgtgag                                                                                                                                 <  2:34360/150‑3 (MQ=255)
                  cGTCATTATGGCTTCCTGTTATGAGAGTTATTTTAATGCCCGTAAAAGCGCAACCTTGTCAGCGTTATATGCGACCTTAATGGCTTGCCAGCCCAGTGCCCGGGCGAGTTTCGCCAACCGCTCACGGCCGACCAATAGTCGCCAgtttct                                                                                                                                >  2:1107640/1‑146 (MQ=255)
                  cGTCATTATGGCTTCCTGTTATGAGAGTTATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTgtag                                                                                                                                <  1:1526773/150‑1 (MQ=255)
                   gTCATTATGGCTTCCTGTTATGAGAGTTATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTgtagt                                                                                                                               >  1:876728/1‑150 (MQ=255)
                   gTCATTATGGCTTCCTGTTATGAGAGTTATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTgtagt                                                                                                                               >  1:488292/1‑150 (MQ=255)
                   gTCATTATGGCTTCCTGTTATGAGAGTTATTGTAATGCCCGTAAAAGCGCATCGTTGGCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGTCGACTATTGGTCGTCAgtgagcg                                                                                                                               <  2:56126/150‑5 (MQ=255)
                       ttATGGCTTCCTGTTATGAGAGTTATTGTAATGCCCGTAAAAGCGCATCGTTGGCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAAcc                                                                                                                           <  2:959923/150‑1 (MQ=255)
                             cTTCCTGTTATGAGAGTTATTGTAATGCCCGTAAAAGCGCATCGTTGTCCGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATCGTCGACAGTGTAGTAACCAGTGCt                                                                                                                     <  2:30901/150‑1 (MQ=255)
                              ttCCTGTTATGAGAGTTATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTc                                                                                                                    >  2:1100230/1‑150 (MQ=255)
                                ccTGTTATGAGAGTTATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCAc                                                                                                                  <  1:943983/150‑1 (MQ=255)
                                   gTTATGAGAGTTATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGAt                                                                                                               >  2:756094/1‑150 (MQ=255)
                                         agagTTATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCAtt                                                                                                         >  2:346786/1‑150 (MQ=255)
                                          gagTTATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGCCCAATAGTCGACAGTGTAGTCACCAGTGCTCACGATACCATtg                                                                                                        >  1:259582/1‑150 (MQ=255)
                                          gagTTATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATtg                                                                                                        >  1:527283/1‑150 (MQ=255)
                                            gTTATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGCCCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATtgtg                                                                                                      >  1:174845/1‑150 (MQ=255)
                                            gTTATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATtgtg                                                                                                      >  1:384300/1‑150 (MQ=255)
                                             ttATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTgg                                                                                                     <  2:1131023/150‑1 (MQ=255)
                                              tATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTggg                                                                                                    <  1:1680159/150‑1 (MQ=255)
                                              tATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGGCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAGCGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTggg                                                                                                    <  1:489398/150‑1 (MQ=255)
                                               aTTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGa                                                                                                   <  2:14803/150‑1 (MQ=255)
                                               aTTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGa                                                                                                   <  2:1673577/150‑1 (MQ=255)
                                                  gTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCTATAGTCGCCAGTGTAGTACCCTGTGCTCACGATTCCATTGTGGGATCa                                                                                                >  2:635104/1‑150 (MQ=255)
                                                  gTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCa                                                                                                <  1:1332966/150‑1 (MQ=255)
                                                  gTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCa                                                                                                >  1:270805/1‑150 (MQ=255)
                                                    aaTGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGc                                                                                              <  1:575506/150‑1 (MQ=255)
                                                    aaTGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGc                                                                                              <  2:698396/150‑1 (MQ=255)
                                                     aTGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGCACTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACAGACGACCAATAGTCGACAGTGTAGTAAGCAGTGCTACCGAAACCCTTGTGGGATCAGCg                                                                                             >  1:1020264/1‑150 (MQ=255)
                                                      tGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGCTACCATTGTGGGATCAGCgc                                                                                            >  1:495808/1‑149 (MQ=255)
                                                       gCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGAc                                                                                           >  2:1597766/1‑150 (MQ=255)
                                                          cGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTCTTGGATCAGCGACCac                                                                                        >  2:1688956/1‑149 (MQ=255)
                                                           gTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCaga                                                                                       >  2:1375893/1‑150 (MQ=255)
                                                             aaaaGCGCAGCGTTGTCAGCGTTATCGGCGAACTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGt                                                                                     <  1:53237/150‑1 (MQ=255)
                                                                aGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTCACCAGTGCTCACGATACCATTGTGGGATCCGCGACCAGAGTtgc                                                                                  >  1:275195/1‑150 (MQ=255)
                                                                    cATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCAATGTGGGATCAGCGACCAGAGTTGCTGCa                                                                              >  2:1488935/1‑150 (MQ=255)
                                                                      gcGTTGTCAGCGTTATCGGCGACCTTACTGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATCGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAAc                                                                            <  2:1247182/149‑1 (MQ=255)
                                                                       cGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACa                                                                           >  2:1567426/1‑150 (MQ=255)
                                                                        gTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACAt                                                                          >  1:1019032/1‑150 (MQ=255)
                                                                        gTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACAt                                                                          <  2:1049101/150‑1 (MQ=255)
                                                                        gTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACAt                                                                          >  1:692425/1‑150 (MQ=255)
                                                                        gTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACAt                                                                          >  2:869413/1‑150 (MQ=255)
                                                                         ttttCATCGTTATCGGCGACCTTAATGTATTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCAATGTGGGATCAGCGACCAGAGTTGCTGCAACAtt                                                                         <  2:1327063/147‑1 (MQ=255)
                                                                           gTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTc                                                                       >  1:769457/1‑150 (MQ=255)
                                                                           gTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTc                                                                       >  1:1612348/1‑150 (MQ=255)
                                                                           gTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTc                                                                       <  1:388855/150‑1 (MQ=255)
                                                                             cAGCGTTATCGGCGATCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCAc                                                                     <  2:122906/150‑1 (MQ=255)
                                                                             cAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGATTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCAc                                                                     <  2:1013926/150‑1 (MQ=255)
                                                                                 gTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGcc                                                                 <  1:651997/150‑2 (MQ=255)
                                                                                 gTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTACGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGcc                                                                 <  1:651998/150‑2 (MQ=255)
                                                                                   tATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGACAAACGATCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTgg                                                               <  2:953551/150‑1 (MQ=255)
                                                                                      cGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGCCCAGCGTTGCTGCAACATTTCACCGCTGGTaa                                                            >  2:361789/1‑150 (MQ=255)
                                                                                      cGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTaa                                                            <  1:391258/150‑1 (MQ=255)
                                                                                                 aTGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATc                                                 >  1:512144/1‑150 (MQ=255)
                                                                                                 aTGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATc                                                 >  1:1724999/1‑150 (MQ=255)
                                                                                                  tGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGAc                                                                                           >  2:261232/1‑107 (MQ=255)
                                                                                                  tGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGAc                                                                                           <  1:261232/107‑1 (MQ=255)
                                                                                                   gTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGt                                               >  1:586802/1‑150 (MQ=255)
                                                                                                      ttGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCAc                                            <  1:1100230/150‑1 (MQ=255)
                                                                                                      ttGCCAGCCCAGTTCCCGGGCGAGTGTCGCCAAACGCTCACTGACGACTAATAGTCGACAGTGTAGTAACCAGTGCTCACGAGACCATTGTGGGCTCCGCGCCCAGAGTTGCTGCCACAGTTCCCCGCTGGTAACCCCGACCAGCggccc                                            >  1:52995/1‑146 (MQ=255)
                                                                                                       ggCCAGCCCAGCTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGAGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTCGTAACAACGACCATCGTCAcc                                           <  2:1476156/149‑1 (MQ=255)
                                                                                                        gCCAGCCGAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCt                                          <  1:1279569/150‑1 (MQ=255)
                                                                                                        gCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCt                                          >  2:1138908/1‑150 (MQ=255)
                                                                                                         ccAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCTc                                         >  2:1489108/1‑150 (MQ=255)
                                                                                                                    cccGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCTCGCGGGCTTGcc                              <  2:384300/150‑1 (MQ=255)
                                                                                                                      cGGGCGAGTGTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCTCGCGGGCTTGCCAg                            <  1:127761/150‑1 (MQ=255)
                                                                                                                        ggCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCTCTGGTAACAACGACCATCTTCACCTCGCGGGCTTGCCAgcg                          <  1:492311/150‑1 (MQ=255)
                                                                                                                           gAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCTCGCTGGCAAGCCcgcgagg                       <  2:40512/150‑4 (MQ=255)
                                                                                                                             gTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCTCGCGGGCTTGCCAGCGCATCg                     >  1:1038145/1‑150 (MQ=255)
                                                                                                                                 cGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCTCGCGGGCTTGCCAGCgcttcgcttc                 >  1:117507/1‑150 (MQ=255)
                                                                                                                                 cGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCTCGCGGGCTTGCCAGCGCATCGcttc                 >  1:817794/1‑150 (MQ=255)
                                                                                                                                 cGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCTCGCGGGCTTGCCAGCGCATCGcttc                 >  1:808978/1‑150 (MQ=255)
                                                                                                                                 cGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCTCGCGGGCTTGCCAGCGCATCGcttc                 >  1:802075/1‑150 (MQ=255)
                                                                                                                                  gCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCTCGCGGGCTTGCCAGCGCATCGcttct                <  2:1296956/150‑1 (MQ=255)
                                                                                                                                   ccAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCTCGCGGGCTTGCCAGCGCATCGcttctt               >  2:54170/1‑150 (MQ=255)
                                                                                                                                      aaCGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCTCGCGGGCTTGCCAGCGCATCGCTTCTTCTg            <  2:1480725/150‑1 (MQ=255)
                                                                                                                                         gCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCTTCACCTCGCTGGCTTGCCAGCGCATCGCTTCTTCTGCAc         >  2:970301/1‑150 (MQ=255)
                                                                                                                                         gCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCTCGCGGGCTTGCCAGCGCATCGCTTCTTCTGCAc         <  1:848790/150‑1 (MQ=255)
                                                                                                                                            cACTGTCGACTATTGGTCGTCAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCTCGCGGGCTTGCCAGCGCATCGCTTCTTCTGCACCAt      >  2:1037647/1‑150 (MQ=255)
                                                                                                                                            cACTGTCGACTATTGGTCGTCAGTGTAGTAACCAGTGATCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCTCGCGGGCTTGCCAGCGCATCGCTTCTTCTGCACCAt      >  1:1170898/1‑150 (MQ=255)
                                                                                                                                            cACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCTCGCGGGCTTGCCAGCGCATCGCTTCTTCTGCACCAt      <  2:270805/150‑1 (MQ=255)
                                                                                                                                             aCTGTCGACTATTGGTCGTCAGTGTAGTAACCAGTGCTCACGATACCAGTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCTCGCGGGCTTGCCAGCGCATCGCTTCTTCTGCACCATc     >  2:118721/1‑150 (MQ=255)
                                                                                                                                              cTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCTCGCGGGCTTGCCAGCGCATCGCTTCTTCTGCACCATCg    >  2:1716122/1‑150 (MQ=255)
                                                                                                                                              cTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCTCGCGGGCTTGCCAGCGCATCGCTTCTTCTGCACCATCg    >  2:411898/1‑150 (MQ=255)
                                                                                                                                               tGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCTCGCGGGCTTGCCAGCGCATCGCTTCTTCTGCACCATCGt   <  2:259582/150‑1 (MQ=255)
                                                                                                                                                gacgccCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCTCGCGGGCTTGCCAGCGCCTCGCTTCTTCTGCAACATCGTa  >  2:187477/1‑150 (MQ=255)
                                                                                                                                                gacgacCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCTCGCGGGCTTGCCAGCGCATCGCTTCTTCTGCACCATCGTa  <  2:16747/150‑1 (MQ=255)
                                                                                                                                                gacgacCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCTCGCGGGCTTGCCAGCGCATCGCTTCTTCTGCACCATCGTa  >  2:486955/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GGAGGTTTTTTCTTGTTCCGTCATTATGGCTTCCTGTTATGAGAGTTATTGTAATGCCCGTAAAAGCGCATCGTTGTCAGCGTTATCGGCGACCTTAATGTCTTGCCAGCCCAGTTCCCGGGCGAGTTTCGCCAAACGCTCACTGACGACCAATAGTCGACAGTGTAGTAACCAGTGCTCACGATACCATTGTGGGATCAGCGACCAGAGTTGCTGCAACATTTCACCGCTGGTAACAACGACCATCGTCACCTCGCGGGCTTGCCAGCGCATCGCTTCTTCTGCACCATCGTA  >  NC_000913/3989043‑3989336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: