Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,049,986 G→T 5.7% noncoding (88/109 nt) spf → Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,049,9860GT 5.7% 372.5 / 8.3 133noncoding (88/109 nt)spfSpot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
Reads supporting (aligned to +/- strand):  ref base G (61/64);  new base T (6/2);  total (67/66)
Fisher's exact test for biased strand distribution p-value = 2.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01

Only 100 of 138 total aligned reads displayed.

ACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCAAGAAAATTA  >  NC_000913/4049838‑4050135
                                                                                                                                                    |                                                                                                                                                      
acTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCcagtcagt                                                                                                                                                       <  1:574536/150‑1 (MQ=255)
 cTGAAAAAAAATTACAAACAGTGCTTTCTGAACTGAACAAAAAAGAGAAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGATCTATCTTTCAGACGTTTTACTTCACGTAAGCCGATTTGGCAGAATATTTTACCCGCCCCAGTCAGTa                                                                                                                                                      >  2:1320517/1‑150 (MQ=255)
   gAAAAAAAATTACAAAAAGTGCTTTCTGACCTGAACAAAAAAGAGTAAAGTTAGACGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCATACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCACAGTCAGTAAt                                                                                                                                                    >  1:339920/1‑150 (MQ=255)
    aaaaaaaaTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGATCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATg                                                                                                                                                   >  1:1727926/1‑150 (MQ=255)
      aaaaaaTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGAc                                                                                                                                                 >  1:1537617/1‑150 (MQ=255)
      aaaaaaTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGAc                                                                                                                                                 >  2:913784/1‑150 (MQ=255)
          aaTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTggg                                                                                                                                             >  2:1125853/1‑150 (MQ=255)
           aTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTgggg                                                                                                                                            <  1:817581/150‑1 (MQ=255)
            ttACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGc                                                                                                                                           <  1:1631878/150‑1 (MQ=255)
              aCAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGGCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCATTACTGACTGGGGCgg                                                                                                                                         <  1:850499/150‑2 (MQ=255)
               cAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGtt                                                                                                                                       >  2:263360/1‑150 (MQ=255)
                aaaaaGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGttt                                                                                                                                      <  1:7894/150‑1 (MQ=255)
                 aaaaGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGtttt                                                                                                                                     >  2:799687/1‑150 (MQ=255)
                  aaaGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGttttt                                                                                                                                    <  2:917166/150‑1 (MQ=255)
                   aaGTGCTTTCTTAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCATTACTGACTGGGGCggctaaa                                                                                                                                    <  1:1168287/150‑7 (MQ=255)
                    aGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTa                                                                                                                                  >  2:1042675/1‑150 (MQ=255)
                    aGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTa                                                                                                                                  <  2:1403716/150‑1 (MQ=255)
                      tGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTAtt                                                                                                                                <  2:677505/150‑1 (MQ=255)
                       gCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTg                                                                                                                               >  2:59283/1‑150 (MQ=255)
                       gCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTg                                                                                                                               <  2:1704144/150‑1 (MQ=255)
                        cTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTgg                                                                                                                              <  2:555848/150‑1 (MQ=255)
                        cTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGGCAGTAATGACTGGGGCGTTTTTTATTgg                                                                                                                              <  1:677271/150‑1 (MQ=255)
                          ttCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGCCCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGc                                                                                                                            >  2:381519/1‑150 (MQ=255)
                          ttCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGc                                                                                                                            <  1:1222774/150‑1 (MQ=255)
                               aacttaacAAAAAAGAGTAAAGTTAGTCGCGTATGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACATCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCgaaag                                                                                                                       <  2:1577736/150‑1 (MQ=255)
                               aactgaacAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCgaaag                                                                                                                       <  2:761045/150‑1 (MQ=255)
                                actgaacAAAAAAGAGTAAAGTTAGTCGCGTAGGGTAGAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGAAATGACTGGGGCGTTTTTTATTGGGCgaaaga                                                                                                                      <  1:247506/150‑1 (MQ=255)
                                actgaacAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCgaaaga                                                                                                                      >  1:314053/1‑150 (MQ=255)
                                   gaacAAAAAAGAGTAAAGGTCGTCGCGTAGGGAACTGAGGTAAGATTCTCTATCATTCAGACCTTTTACTACACGAAAACGGATTTGGCAGAATATTTAAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGaaagaaaa                                                                                                                   <  1:281513/150‑1 (MQ=255)
                                    aacAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGATGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGaaagaaaag                                                                                                                  <  2:986589/150‑1 (MQ=255)
                                     acAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTATATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGTGCGaaagaaaaga                                                                                                                 >  1:106954/1‑150 (MQ=255)
                                      cAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGAt                                                                                                                >  1:1717865/1‑150 (MQ=255)
                                      cAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGAATGGGGCGTTTTTTATTTGGCGAAAGAAAAGAt                                                                                                                >  1:1616715/1‑150 (MQ=255)
                                      cAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTATACTTCACGTAATCGGATTTGTCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTCGGGCGTTTTTTATTGGGCGAAATAAAAGAt                                                                                                                >  2:1725863/1‑150 (MQ=255)
                                        aaaaaGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATcc                                                                                                              <  1:835507/150‑1 (MQ=255)
                                        aaaaaGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATcc                                                                                                              <  2:1219954/150‑1 (MQ=255)
                                          aaaGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGt                                                                                                            <  1:142982/150‑1 (MQ=255)
                                             gagTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGACGCCCCAGTCAGTAATGCCTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAAt                                                                                                         >  1:1060727/1‑150 (MQ=255)
                                              agTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATg                                                                                                        <  1:1124670/150‑1 (MQ=255)
                                                 aaaGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGccg                                                                                                     >  2:367034/1‑149 (MQ=255)
                                                 aaaGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGAATGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCt                                                                                                     >  2:63459/1‑150 (MQ=255)
                                                     ttAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATg                                                                                                 <  2:1636614/150‑1 (MQ=255)
                                                       aGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATgcg                                                                                               >  1:381541/1‑150 (MQ=255)
                                                         tCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCt                                                                                             <  1:607295/150‑1 (MQ=255)
                                                         tCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCt                                                                                             <  1:1175263/150‑1 (MQ=255)
                                                          cgcgTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTa                                                                                            >  1:373938/1‑150 (MQ=255)
                                                            cgTAGGGTACAGAGGTAAGATGTTCTATCTTTAAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATg                                                                                          >  2:991704/1‑150 (MQ=255)
                                                             gTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGt                                                                                         <  1:1415795/150‑1 (MQ=255)
                                                              tAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGtt                                                                                        <  2:459205/150‑1 (MQ=255)
                                                              tAGGGTACAGAGGTAAGATGTTCTATCTATCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGtt                                                                                        >  2:1187580/1‑150 (MQ=255)
                                                                gggTACAGTGGTATGTTGTTCTATATTTAAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTa                                                                                      <  2:1155459/150‑1 (MQ=255)
                                                                gggTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTa                                                                                      >  2:1393063/1‑150 (MQ=255)
                                                                    aCAGAGGTAAGATGTTCTATCTTTCAGACTTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAg                                                                                  <  2:701497/150‑1 (MQ=255)
                                                                     cAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAgg                                                                                 >  1:523199/1‑150 (MQ=255)
                                                                         ggTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCaa                                                                             >  2:1349631/1‑150 (MQ=255)
                                                                         ggTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGAATCGCTAAGTTTATCAGGCCaa                                                                             >  1:1269382/1‑150 (MQ=255)
                                                                            aaGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGTCTGAATATTTTAGCCTCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACgg                                                                          <  1:1087632/150‑1 (MQ=255)
                                                                            aaGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACgg                                                                          >  2:1622236/1‑150 (MQ=255)
                                                                                 gTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTATATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGaa                                                                     >  1:1166100/1‑150 (MQ=255)
                                                                                   tCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAAtt                                                                   >  2:1458921/1‑150 (MQ=255)
                                                                                     tATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGGCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGt                                                                 <  2:1727926/150‑1 (MQ=255)
                                                                                         tttCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATc                                                             >  1:933161/1‑150 (MQ=255)
                                                                                              gACCTTTTACTTCACGTAATCGTATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCTAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTg                                                        <  1:1353505/150‑1 (MQ=255)
                                                                                              gACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTg                                                        <  1:59283/150‑1 (MQ=255)
                                                                                               aCCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGa                                                       >  1:1019188/1‑150 (MQ=255)
                                                                                                ccTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGaa                                                      >  1:1710808/1‑150 (MQ=255)
                                                                                                      aCTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTAc                                                 >  2:349753/1‑150 (MQ=255)
                                                                                                        ttCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACggg                                              <  1:1042675/150‑1 (MQ=255)
                                                                                                         tCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATAAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGc                                             >  2:1304563/1‑150 (MQ=255)
                                                                                                          cACGTAATAGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAATAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGATTTTACGGGcc                                            >  1:100171/1‑150 (MQ=255)
                                                                                                           aCGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCCACGGTAGAATTGTAACCTATTGAATTTAAGGGCCg                                           >  1:1536879/1‑150 (MQ=255)
                                                                                                           aCGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCg                                           <  2:1337833/150‑1 (MQ=255)
                                                                                                            cGTAATCGGATTTGGCTGAATATTTAATCCGACCCAGTCAGTAATGAGTGGGGGGTTTTTTATTGGGCGAAAGATAAGATCCGTAATGCATGATGCGCTAGGTTTATCAGGCCTACGGTAGAATTGTAATCTATTGAATTTACGTGCCgg                                          <  1:464747/150‑1 (MQ=255)
                                                                                                                 tCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCGATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTTAATTTACGGGCCGGATACg                                     >  1:1550766/1‑150 (MQ=255)
                                                                                                                 tCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGGTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTTCGGGCCGGATACg                                     >  1:883985/1‑150 (MQ=255)
                                                                                                                   ggATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGcc                                   >  2:1048360/1‑150 (MQ=255)
                                                                                                                    gATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCca                                  >  2:1315337/1‑150 (MQ=255)
                                                                                                                     aTTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCcac                                 <  2:383946/150‑1 (MQ=255)
                                                                                                                        tGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATc                              >  1:896279/1‑150 (MQ=255)
                                                                                                                         ggCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATcc                             <  2:1188714/150‑1 (MQ=255)
                                                                                                                          gCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCg                            >  1:521403/1‑150 (MQ=255)
                                                                                                                           cTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCgg                           >  2:1632423/1‑150 (MQ=255)
                                                                                                                            ttaaTATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGc                          <  2:1504299/148‑1 (MQ=255)
                                                                                                                              aaTATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGcac                        <  1:379467/150‑1 (MQ=255)
                                                                                                                                  ttttAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTTTGTTTATCAGGCCAACGGTAGAATTGTATTCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGc                    >  1:1403526/1‑150 (MQ=255)
                                                                                                                                      aGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTa                >  2:1122511/1‑150 (MQ=255)
                                                                                                                                      aGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATAGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCATGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGTATACGCCACATCCGGCACAAGCATTa                <  1:1003421/150‑1 (MQ=255)
                                                                                                                                        ccgccCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAg              <  1:1263428/150‑1 (MQ=255)
                                                                                                                                        ccgccCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGCAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAg              <  1:1349631/150‑1 (MQ=255)
                                                                                                                                         cgccCCAGTCATTACTGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAgg             >  2:502005/1‑150 (MQ=255)
                                                                                                                                         cgccCCAGTCATGACTGAGGGGGGCGTTTTTTATTGGGCGAATGAAAAGATCCGTAATGCCTGATGCGCTTTGGTTATCAGGGCACCGGTAGAATTGTAGTCTATTGATTTTACGGGCCGGGTACGCCCCCGCCGGCACGCGCAGTAAgg             >  2:462685/1‑150 (MQ=255)
                                                                                                                                         cgccCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAgg             <  1:330836/150‑1 (MQ=255)
                                                                                                                                         cgccCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAgg             >  1:4533/1‑150 (MQ=255)
                                                                                                                                          gccCCAGTCATTACTGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGc            >  2:38814/1‑150 (MQ=255)
                                                                                                                                           ccccAGTCATTACTGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCa           >  2:202981/1‑150 (MQ=255)
                                                                                                                                           ccccAGTCATTACTGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCa           >  2:223989/1‑150 (MQ=255)
                                                                                                                                           ccccAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCa           <  1:768149/150‑1 (MQ=255)
                                                                                                                                                   agtAATTACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCAAGAAAatt   <  2:1688622/150‑1 (MQ=255)
                                                                                                                                                   agtAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCAAGAAAatt   >  2:695788/1‑150 (MQ=255)
                                                                                                                                                    gtAATGACTGGGGCGTTTTTTATTGGGCCCAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAAATTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCAAGAAAatta  <  2:323768/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
ACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCAAGAAAATTA  >  NC_000913/4049838‑4050135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: