Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,049,989 | A→C | 5.6% | noncoding (91/109 nt) | spf → | Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,049,989 | 0 | A | C | 5.6% | 399.2 / 10.4 | 136 | noncoding (91/109 nt) | spf | Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent |
Reads supporting (aligned to +/- strand): ref base A (61/67); new base C (6/2); total (67/69) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.62e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.37e-01 |
Only 100 of 138 total aligned reads displayed.
GAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCAAGAAAATTATT > NC_000913/4049841‑4050137 | aaaaaaTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGAc > 1:1537617/1‑150 (MQ=255) aaaaaaTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGAc > 2:913784/1‑150 (MQ=255) aaTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTggg > 2:1125853/1‑150 (MQ=255) aTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTgggg < 1:817581/150‑1 (MQ=255) ttACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGc < 1:7525/150‑1 (MQ=255) ttACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGc < 1:1631878/150‑1 (MQ=255) cAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑tt > 2:263360/1‑150 (MQ=255) aaaaGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑tttt > 2:799687/1‑150 (MQ=255) aaaGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑ttttt < 2:917166/150‑1 (MQ=255) aaGTGCTTTCTTAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCATTACTGACTGGGGCggctaaa < 1:1168287/150‑7 (MQ=255) aGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTa > 2:1042675/1‑150 (MQ=255) aGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTa < 2:1403716/150‑1 (MQ=255) tGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTAtt < 2:677505/150‑1 (MQ=255) gCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTg < 2:1704144/150‑1 (MQ=255) gCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTg > 2:59283/1‑150 (MQ=255) cTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTgg < 2:555848/150‑1 (MQ=255) ttCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGCCCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGc > 2:381519/1‑150 (MQ=255) ttCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGc < 1:1222774/150‑1 (MQ=255) aacttaacAAAAAAGAGTAAAGTTAGTCGCGTATGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACATCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCgaaag < 2:1577736/150‑1 (MQ=255) aactgaacAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCgaaag < 2:1204857/150‑1 (MQ=255) aactgaacAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCgaaag < 2:761045/150‑1 (MQ=255) actgaacAAAAAAGAGTAAAGTTAGTCGCGTAGGGTAGAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGAAATGACTGGGGCG‑TTTTTTATTGGGCgaaaga < 1:247506/150‑1 (MQ=255) actgaacAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCgaaaga > 1:314053/1‑150 (MQ=255) aacAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGATGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGaaagaaaag < 2:986589/150‑1 (MQ=255) aaaaaaaaaGATTAAATTTAGGCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGTATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGaaagaaaag < 2:1095886/147‑1 (MQ=255) acAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTATATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGTGCGaaagaaaaga > 1:106954/1‑150 (MQ=255) cAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGAt > 1:1717865/1‑150 (MQ=255) cAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGAATGGGGCG‑TTTTTTATTTGGCGAAAGAAAAGAt > 1:1616715/1‑150 (MQ=255) cAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTATACTTCACGTAATCGGATTTGTCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTCGGGCG‑TTTTTTATTGGGCGAAATAAAAGAt > 2:1725863/1‑150 (MQ=255) aaaaaGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATcc < 1:835507/150‑1 (MQ=255) aaaaaGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATcc < 2:1219954/150‑1 (MQ=255) aaaGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGt < 1:142982/150‑1 (MQ=255) gagTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGACGCCCCAGTCAGTAATGCCTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAAt > 1:1060727/1‑150 (MQ=255) agTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATg < 1:1124670/150‑1 (MQ=255) aaaGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGccg > 2:367034/1‑149 (MQ=255) aaaGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGAATGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCt > 2:63459/1‑150 (MQ=255) aGTTAGGCGCGTAGGGTACTGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATTTTTTAGACTCACCAGTCAGTAATTACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGa < 2:1174711/150‑1 (MQ=255) gTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGAt > 1:410635/1‑150 (MQ=255) ttAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATg < 2:1636614/150‑1 (MQ=255) tCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCt < 1:1175263/150‑1 (MQ=255) tCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCt < 1:607295/150‑1 (MQ=255) cgcgTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTa > 1:373938/1‑150 (MQ=255) ggTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATg < 2:1319424/149‑1 (MQ=255) cgTAGGGTACAGAGGTAAGATGTTCTATCTTTAAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATg > 2:991704/1‑150 (MQ=255) gTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGt < 1:1415795/150‑1 (MQ=255) tAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGtt < 2:459205/150‑1 (MQ=255) tAGGGTACAGAGGTAAGATGTTCTATCTATCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGtt > 2:1187580/1‑150 (MQ=255) gggTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTa > 2:1393063/1‑150 (MQ=255) aCAGAGGTAAGATGTTCTATCTTTCAGACTTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAg < 2:701497/150‑1 (MQ=255) cAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAgg > 1:523199/1‑150 (MQ=255) ggTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCaa > 2:1349631/1‑150 (MQ=255) ggTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGAATCGCTAAGTTTATCAGGCCaa > 1:1269382/1‑150 (MQ=255) tAAGATGTTCTATCTTTCAGACCTTTTACTTCACGAAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACg < 1:212512/150‑1 (MQ=255) aaGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGTCTGAATATTTTAGCCTCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACgg < 1:1087632/150‑1 (MQ=255) aaGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACgg > 2:1622236/1‑150 (MQ=255) gTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTATATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGaa > 1:1166100/1‑150 (MQ=255) tCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAAtt > 2:1458921/1‑150 (MQ=255) tATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGGCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGt < 2:1727926/150‑1 (MQ=255) tttCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATc > 1:933161/1‑150 (MQ=255) gACCTTTTACTTCACGTAATCGTATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCTAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTg < 1:1353505/150‑1 (MQ=255) gACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTg < 1:59283/150‑1 (MQ=255) aCCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGa > 1:1019188/1‑150 (MQ=255) aCCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGa < 1:490564/150‑1 (MQ=255) ccTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGaa > 1:1710808/1‑150 (MQ=255) aCTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTAc > 2:349753/1‑150 (MQ=255) aCTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACg < 1:799687/150‑1 (MQ=255) cTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGTGGCG‑TTTTTTATTGGGCGAAAGAAAATATACGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACgg < 1:1676019/150‑1 (MQ=255) ttCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACggg < 1:1042675/150‑1 (MQ=255) tCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGc < 1:37130/150‑1 (MQ=255) tCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATAAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGc > 2:1304563/1‑150 (MQ=255) cACGTAATAGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAATAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGATTTTACGGGcc > 1:100171/1‑150 (MQ=255) aCGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCCACGGTAGAATTGTAACCTATTGAATTTAAGGGCCg > 1:1536879/1‑150 (MQ=255) aCGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCg < 2:1337833/150‑1 (MQ=255) aTCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATAc < 1:919413/150‑1 (MQ=255) tCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCGATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTTAATTTACGGGCCGGATACg > 1:1550766/1‑150 (MQ=255) tCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑GTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTTCGGGCCGGATACg > 1:883985/1‑150 (MQ=255) aTTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCcac < 2:383946/150‑1 (MQ=255) gCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCg > 1:521403/1‑150 (MQ=255) cTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCgg > 2:1632423/1‑150 (MQ=255) cTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCgg < 1:284419/150‑1 (MQ=255) ttaaTATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGc < 2:1504299/148‑1 (MQ=255) aaTATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGcac < 1:379467/150‑1 (MQ=255) ttttAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTTTGTTTATCAGGCCAACGGTAGAATTGTATTCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGc > 1:1403526/1‑150 (MQ=255) ttttAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TGTTTTATTGGGCGAAAGAAAAGAACCGGAATGCCTGATGGGCGTTGTGTATCAGGCCAACGTGAGAATTTTAATCATTTTAATTTACGGGCCGGATACGCCCCATACGGCACAAga > 1:876859/1‑149 (MQ=255) aGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTa > 2:1122511/1‑150 (MQ=255) aGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATAGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCATGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGTATACGCCACATCCGGCACAAGCATTa < 1:1003421/150‑1 (MQ=255) ccgccCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAg < 1:1263428/150‑1 (MQ=255) ccgccCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGCAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAg < 1:1349631/150‑1 (MQ=255) cgccCCAGTCATTACTGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAgg > 2:502005/1‑150 (MQ=255) cgccCCAGTCATGACTGAGGGGGGCG‑TTTTTTATTGGGCGAATGAAAAGATCCGTAATGCCTGATGCGCTTTGGTTATCAGGGCACCGGTAGAATTGTAGTCTATTGATTTTACGGGCCGGGTACGCCCCCGCCGGCACGCGCAGTAAgg > 2:462685/1‑150 (MQ=255) cgccCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAgg > 1:4533/1‑150 (MQ=255) cgccCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAgg < 1:330836/150‑1 (MQ=255) gccCCAGTCATTACTGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGc > 2:38814/1‑150 (MQ=255) ccccAGTCATTACTGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCa > 2:223989/1‑150 (MQ=255) ccccAGTCATTACTGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCa > 2:202981/1‑150 (MQ=255) ccccAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCa < 1:768149/150‑1 (MQ=255) agtAATTACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCAAGAAAatt < 2:1688622/150‑1 (MQ=255) agtAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCAAGAAAatt > 2:695788/1‑150 (MQ=255) gtAATGACTGGGGCG‑TTTTTTATTGGGCCCAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAAATTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCAAGAAAatta < 2:323768/150‑1 (MQ=255) aaTGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCAAGAAAattatt < 1:63459/150‑1 (MQ=255) | GAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCG‑TTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTTTATCAGGCCAACGGTAGAATTGTAATCTATTGAATTTACGGGCCGGATACGCCACATCCGGCACAAGCATTAAGGCAAGAAAATTATT > NC_000913/4049841‑4050137 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |