Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,080,027 G→A 8.6% intergenic (+58/+272) yiiF → / ← fdhE putative thymol sensitivity protein, CopG family putative transcriptional regulator/formate dehydrogenase formation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,080,0270GA 8.6% 449.9 / 20.7 153intergenic (+58/+272)yiiF/fdhEputative thymol sensitivity protein, CopG family putative transcriptional regulator/formate dehydrogenase formation protein
Reads supporting (aligned to +/- strand):  ref base G (65/73);  new base A (5/8);  total (70/83)
Fisher's exact test for biased strand distribution p-value = 7.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.31e-01

Only 100 of 156 total aligned reads displayed.

GCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGT  >  NC_000913/4079879‑4080173
                                                                                                                                                    |                                                                                                                                                  
gCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATgc                                                                                                                                                   >  2:856985/1‑150 (MQ=255)
  aaaCAGCAAGAACCAGTGTTACTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCTCGTTGTTCATGCCGGATgcgc                                                                                                                                                 >  1:1443069/1‑149 (MQ=255)
   aaCAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATgcggc                                                                                                                                                <  2:449600/150‑1 (MQ=255)
     cAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGt                                                                                                                                              >  1:279481/1‑150 (MQ=255)
      aGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTg                                                                                                                                             <  1:898366/150‑1 (MQ=255)
       gCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGa                                                                                                                                            >  2:1482985/1‑150 (MQ=255)
        cAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGaa                                                                                                                                           >  2:1132655/1‑150 (MQ=255)
        cAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGaa                                                                                                                                           >  2:1608088/1‑150 (MQ=255)
        cAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGaa                                                                                                                                           >  2:593934/1‑150 (MQ=255)
        cAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATAAGGCGttca                                                                                                                                           <  2:861739/150‑4 (MQ=255)
         aaGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAAc                                                                                                                                          >  1:1324129/1‑150 (MQ=255)
         aaGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCACGCCGCATCCGGCATGAAc                                                                                                                                          <  1:1732403/150‑1 (MQ=255)
           gAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGc                                                                                                                                        >  2:1344011/1‑150 (MQ=255)
            aaCCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGcc                                                                                                                                       <  1:1410191/150‑1 (MQ=255)
            aaCCAGTGATCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGcc                                                                                                                                       <  1:673828/150‑1 (MQ=255)
             aCCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCt                                                                                                                                      >  2:735315/1‑150 (MQ=255)
             aCCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCt                                                                                                                                      >  1:1158666/1‑150 (MQ=255)
               cAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATAAGGCGTTCACGccgca                                                                                                                                    <  1:1198563/150‑4 (MQ=39)
                    ttCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCgg                                                                                                                               >  1:557738/1‑150 (MQ=255)
                    ttCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCgg                                                                                                                               <  1:1104051/150‑1 (MQ=255)
                    ttCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATAAGGCGTTCACGCCGCATCCgg                                                                                                                               <  2:763167/150‑1 (MQ=39)
                    ttCCTGGCAGCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCgg                                                                                                                               <  2:436466/150‑1 (MQ=255)
                     tCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                              <  1:1278300/150‑1 (MQ=255)
                      ccTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                             <  2:1725340/150‑1 (MQ=255)
                      ccTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGca                                                                                                                             <  2:134935/150‑2 (MQ=38)
                       cTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGcat                                                                                                                            <  1:1391596/150‑3 (MQ=38)
                       cTGGCATCTGGCAAGGGGGTGAGGAAGATGGTGTCGAATATCACCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCt                                                                                                                            <  2:911907/150‑1 (MQ=255)
                        tGGCATCTGGCAAGGGTGTGAGGAAGATGTTGTCGAATATCAGTGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCTGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGcatg                                                                                                                           <  2:193048/150‑4 (MQ=38)
                        tGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTa                                                                                                                           <  1:452228/150‑1 (MQ=255)
                         ggCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAc                                                                                                                          >  1:441810/1‑150 (MQ=255)
                              cTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGt                                                                                                                     <  1:1274557/150‑1 (MQ=255)
                              cTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGt                                                                                                                     >  2:267403/1‑150 (MQ=255)
                                ggCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCg                                                                                                                   >  2:1688019/1‑150 (MQ=255)
                                ggCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCg                                                                                                                   >  2:66127/1‑150 (MQ=255)
                                   aggggTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGtat                                                                                                                <  2:531769/148‑1 (MQ=255)
                                     gggTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATaa                                                                                                              >  1:774128/1‑150 (MQ=255)
                                       gtgtGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTAGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCCTATAAAt                                                                                                            >  2:449614/1‑150 (MQ=255)
                                         gtgAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTc                                                                                                          >  1:902317/1‑150 (MQ=255)
                                             ggAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATg                                                                                                      >  2:1275750/1‑150 (MQ=255)
                                                 gATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTAtt                                                                                                  <  2:505462/150‑1 (MQ=255)
                                                   tggtggAGAATATCAGCGTACGCTGCGCGAGGCATGGTAATGGGAAAAAATGATTTCAATCAGATTGCTGACACCGTGCGCGTTGTTCATGCCGGAGGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGc                                                                                                <  2:89832/150‑1 (MQ=25)
                                                   tGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGc                                                                                                <  1:6204/150‑1 (MQ=255)
                                                     gtgtCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAg                                                                                              >  2:1114079/1‑150 (MQ=255)
                                                         cGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTa                                                                                          >  1:343054/1‑150 (MQ=255)
                                                          gAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTAt                                                                                         <  1:669906/150‑1 (MQ=255)
                                                            atatCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTg                                                                                       >  2:249930/1‑150 (MQ=255)
                                                              atCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGt                                                                                     >  1:794224/1‑150 (MQ=255)
                                                                cAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCGTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAg                                                                                   >  2:1382836/1‑150 (MQ=255)
                                                                 aGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCGGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAgg                                                                                  <  2:410793/150‑1 (MQ=255)
                                                                 aGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAgg                                                                                  >  1:1522723/1‑150 (MQ=255)
                                                                  gCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGCCAACGTGCGAGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGGTTATTGGTAGGc                                                                                 >  2:1073696/1‑150 (MQ=255)
                                                                  gCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGc                                                                                 <  1:1609132/150‑1 (MQ=255)
                                                                    gTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCt                                                                               <  2:1312058/150‑1 (MQ=255)
                                                                        tcTGCGCGAGGAATGTTAATGGGTATAAATTATTTCAATCAGATTGCTGAGAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGACTACAAAGTAGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATa                                                                           <  1:302746/149‑1 (MQ=25)
                                                                            cgcgAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAagcg                                                                       >  1:1343389/1‑150 (MQ=255)
                                                                             gcgAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAagcgc                                                                      >  1:1012328/1‑150 (MQ=255)
                                                                             gcgAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGACTACAAAGTCGAATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAaacgc                                                                      >  1:1106329/1‑150 (MQ=255)
                                                                                aGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAagcgcagc                                                                   >  1:1596165/1‑150 (MQ=255)
                                                                                aGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAagcgcagc                                                                   >  1:445576/1‑150 (MQ=255)
                                                                                 ggAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAagcgcagcg                                                                  >  1:1500054/1‑150 (MQ=255)
                                                                                  gAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAagcgcagcgc                                                                 <  2:155886/150‑1 (MQ=255)
                                                                                   aaTGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAgcgcagcgca                                                                <  1:891659/150‑1 (MQ=255)
                                                                                      ggTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCa                                                             >  1:1718727/1‑150 (MQ=255)
                                                                                         aaTGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGc                                                          <  1:1019717/150‑1 (MQ=255)
                                                                                         aaTGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGc                                                          >  2:1068132/1‑150 (MQ=255)
                                                                                            gggTAAAAATGATGTCAATCAGATTGCAGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATGCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGGAGCGCATCAGGCAAt                                                       <  2:1503732/150‑1 (MQ=255)
                                                                                              gAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCt                                                     >  1:470298/1‑150 (MQ=255)
                                                                                                 aaaaTGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTAc                                                  >  1:1452383/1‑150 (MQ=255)
                                                                                                 aaaaTGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTAc                                                  <  2:557738/150‑1 (MQ=255)
                                                                                                        tgTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGtc                                           >  2:1006812/1‑150 (MQ=255)
                                                                                                         gTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGtca                                          >  2:316621/1‑150 (MQ=255)
                                                                                                            aaTCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGtcatca                                       >  1:601338/1‑150 (MQ=255)
                                                                                                             aTCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAg                                      <  2:1216204/150‑1 (MQ=255)
                                                                                                               cAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGtc                                    >  2:1498296/1‑150 (MQ=255)
                                                                                                               cAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCAGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGtc                                    >  1:629271/1‑150 (MQ=255)
                                                                                                               cAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCAGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGtc                                    <  1:1499262/150‑1 (MQ=255)
                                                                                                                    tGCTGAAAACGTGCGCGGTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATg                               <  2:635976/150‑1 (MQ=255)
                                                                                                                    catgaaCAACGCGCACGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATg                               >  1:1522677/6‑150 (MQ=255)
                                                                                                                      cTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGcc                             >  2:396601/1‑150 (MQ=255)
                                                                                                                      cTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGcc                             <  1:293899/150‑1 (MQ=255)
                                                                                                                      cTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTAATGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGTCAATCTTACGCTTGTCATCAGTCTCATGcc                             >  2:1577574/1‑150 (MQ=255)
                                                                                                                        gACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCa                           >  1:1114690/1‑150 (MQ=255)
                                                                                                                         aCAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCAt                          >  1:1071739/1‑150 (MQ=255)
                                                                                                                             cGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAAcc                      <  2:1324129/150‑1 (MQ=255)
                                                                                                                             cGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAAcc                      >  2:1415043/1‑150 (MQ=255)
                                                                                                                                 cgcgTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTAt                  <  2:586273/150‑1 (MQ=255)
                                                                                                                                 cgcgTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTATATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTAt                  <  1:982745/150‑1 (MQ=255)
                                                                                                                                   cgTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTg                >  1:513914/1‑150 (MQ=255)
                                                                                                                                      tgttCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGGCGTATAAATTAAATGTGTTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCAGCAGGCAATCTTACGCTTGTCATCAGGCTCATGCCCATAACCTTATTGAtg             <  2:720536/150‑1 (MQ=37)
                                                                                                                                          taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAAGTCAATGTATTGCAGTTTAGTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATttggt         >  1:1240922/4‑150 (MQ=37)
                                                                                                                                          cATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTAtt                 >  1:360535/1‑142 (MQ=255)
                                                                                                                                          cATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATgtggt         <  1:1608088/150‑1 (MQ=255)
                                                                                                                                          aaTGCCGGATTCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATgtggt         <  1:131568/149‑1 (MQ=255)
                                                                                                                                           aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATgtggtg        >  2:1144958/3‑150 (MQ=38)
                                                                                                                                           aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATgtggtg        >  2:1163331/3‑150 (MQ=38)
                                                                                                                                           aTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTTAAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATATTACGCTTGTCATCAGTCTCATGCCCATATCCTTATTGATgtggtg        >  2:354078/1‑150 (MQ=255)
                                                                                                                                           aTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTAGTGCAGGTTAGTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCTTCAGTCTCATGCCCATAACCTTATTGCTgtggtg        >  1:1057357/1‑150 (MQ=25)
                                                                                                                                            ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTTCGCTGGTCATCAGTCTCATGCCCATAACCTTAGTGATgtggtgg       >  2:121995/2‑150 (MQ=37)
                                                                                                                                              ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATgtggtggtg     <  2:1619837/150‑1 (MQ=255)
                                                                                                                                                 gATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGt  <  1:1114079/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGT  >  NC_000913/4079879‑4080173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: