Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,080,034 G→T 7.1% intergenic (+65/+265) yiiF → / ← fdhE putative thymol sensitivity protein, CopG family putative transcriptional regulator/formate dehydrogenase formation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,080,0340GT 7.1% 445.0 / 19.0 156intergenic (+65/+265)yiiF/fdhEputative thymol sensitivity protein, CopG family putative transcriptional regulator/formate dehydrogenase formation protein
Reads supporting (aligned to +/- strand):  ref base G (69/76);  new base T (5/6);  total (74/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.28e-02

Only 100 of 159 total aligned reads displayed.

AGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAATTGAGGC  >  NC_000913/4079885‑4080183
                                                                                                                                                     |                                                                                                                                                     
aGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTg                                                                                                                                                       <  1:898366/150‑1 (MQ=255)
 gCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGa                                                                                                                                                      >  2:1482985/1‑150 (MQ=255)
  cAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGaa                                                                                                                                                     >  2:1601800/1‑150 (MQ=255)
  cAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGaa                                                                                                                                                     >  2:1608088/1‑150 (MQ=255)
  cAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGaa                                                                                                                                                     >  2:1132655/1‑150 (MQ=255)
   aaGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAAc                                                                                                                                                    >  1:1324129/1‑150 (MQ=255)
      aaCCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGcc                                                                                                                                                 <  1:1410191/150‑1 (MQ=255)
      aaCCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGAGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGAAGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGcc                                                                                                                                                 <  1:142776/150‑1 (MQ=255)
      aaCCAGTGATCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGcc                                                                                                                                                 <  1:673828/150‑1 (MQ=255)
       aCCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCt                                                                                                                                                >  2:735315/1‑150 (MQ=255)
       aCCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCt                                                                                                                                                >  1:1158666/1‑150 (MQ=255)
         cAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTa                                                                                                                                              <  1:1541630/150‑1 (MQ=255)
         cAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATAAGGCGTTCACGccgca                                                                                                                                              <  1:1198563/150‑4 (MQ=39)
             gtTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCg                                                                                                                                          >  2:133607/1‑150 (MQ=255)
              ttCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCgg                                                                                                                                         <  1:1104051/150‑1 (MQ=255)
              ttCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCgg                                                                                                                                         >  1:557738/1‑150 (MQ=255)
              ttCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATAAGGCGTTCACGCCGCATCCgg                                                                                                                                         <  2:763167/150‑1 (MQ=39)
               tCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                                        <  1:1278300/150‑1 (MQ=255)
               tCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                                        <  1:1520399/150‑1 (MQ=255)
                gcTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTGGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGca                                                                                                                                       <  2:472153/149‑2 (MQ=38)
                ccTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                       <  2:1725340/150‑1 (MQ=255)
                ccTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGca                                                                                                                                       <  2:134935/150‑2 (MQ=38)
                ccTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATAGCAGCGTCAGCTGCGCGAGGAATGGTACTGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                       <  1:1124112/150‑1 (MQ=255)
                 cTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGcat                                                                                                                                      <  1:1391596/150‑3 (MQ=38)
                  tGGCATCTGGCAAGGGTGTGAGGAAGATGTTGTCGAATATCAGTGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCTGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGcatg                                                                                                                                     <  2:193048/150‑4 (MQ=38)
                    gCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACa                                                                                                                                   <  2:1308734/150‑1 (MQ=255)
                        cTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGt                                                                                                                               <  1:1274557/150‑1 (MQ=255)
                        cTGGCAAGGGTGTGAGGAAGATGGTGGCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGt                                                                                                                               <  1:1281367/150‑1 (MQ=255)
                             aaGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGtat                                                                                                                          <  1:1482985/150‑1 (MQ=255)
                                   gtgAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTc                                                                                                                    >  1:902317/1‑150 (MQ=255)
                                       ggAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATg                                                                                                                >  2:149058/1‑150 (MQ=255)
                                       ggAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATg                                                                                                                >  2:1275750/1‑150 (MQ=255)
                                           gATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTAtt                                                                                                            <  2:505462/150‑1 (MQ=255)
                                             tggtggAGAATATCAGCGTACGCTGCGCGAGGCATGGTAATGGGAAAAAATGATTTCAATCAGATTGCTGACACCGTGCGCGTTGTTCATGCCGGAGGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGc                                                                                                          <  2:89832/150‑1 (MQ=25)
                                             tGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGc                                                                                                          <  2:41416/150‑1 (MQ=255)
                                             tGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGc                                                                                                          <  1:6204/150‑1 (MQ=255)
                                                    gAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTAt                                                                                                   >  2:1673418/1‑150 (MQ=255)
                                                    gAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTAt                                                                                                   >  2:1716726/1‑150 (MQ=255)
                                                      atatCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTg                                                                                                 >  2:249930/1‑150 (MQ=255)
                                                        atCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGt                                                                                               >  1:794224/1‑150 (MQ=255)
                                                         tCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTa                                                                                              <  1:1713429/150‑1 (MQ=255)
                                                         tCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTa                                                                                              >  2:1075283/1‑150 (MQ=255)
                                                          cAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCGTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAg                                                                                             >  2:1382836/1‑150 (MQ=255)
                                                           aGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCGGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAgg                                                                                            <  2:410793/150‑1 (MQ=255)
                                                            gCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGCCAACGTGCGAGTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGGTTATTGGTAGGc                                                                                           >  2:1073696/1‑150 (MQ=255)
                                                             cGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACACCGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGcc                                                                                          >  2:1217199/1‑150 (MQ=255)
                                                             cGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGcc                                                                                          <  1:65800/150‑1 (MQ=255)
                                                              gTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGCCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCt                                                                                         <  1:472288/150‑1 (MQ=255)
                                                               tAAGATGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTg                                                                                        <  2:589524/150‑1 (MQ=255)
                                                                  tcTGCGCGAGGAATGTTAATGGGTATAAATTATTTCAATCAGATTGCTGAGAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGACTACAAAGTAGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATa                                                                                     <  1:302746/149‑1 (MQ=25)
                                                                     gcgcGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAagc                                                                                  <  1:409442/150‑1 (MQ=255)
                                                                      cgcgAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACACCGTGCGCGTTGTTCATGCCGGATGCGGCGTGCACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAagcg                                                                                 >  2:277514/1‑150 (MQ=255)
                                                                       gcgAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAagcgc                                                                                >  1:1012328/1‑150 (MQ=255)
                                                                       gcgAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGACTACAAAGTCGAATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAaacgc                                                                                >  1:1106329/1‑150 (MQ=255)
                                                                          aGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAagcgcagc                                                                             >  1:445576/1‑150 (MQ=255)
                                                                           ggtattgTATTGGGAAAAAATGTTGTCAATCAGATTGCTTACAATGTGCGCGTGGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAGAAAGTCGTTAAATTTCAATGTATTGCTGTTTATTTGGAGGCCTGATAagcgcagcg                                                                            <  2:1141393/144‑1 (MQ=21)
                                                                            gAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAagcgcagcgc                                                                           <  2:155886/150‑1 (MQ=255)
                                                                             aaTGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAgcgcagcgca                                                                          <  1:891659/150‑1 (MQ=255)
                                                                             aaTGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAgcgcagcgca                                                                          >  1:686650/1‑150 (MQ=255)
                                                                                ggTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCa                                                                       >  1:1718727/1‑150 (MQ=255)
                                                                                   aaTGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGc                                                                    <  1:1019717/150‑1 (MQ=255)
                                                                                      gggTAAAAATGATGTCAATCAGATTGCAGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATGCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGGAGCGCATCAGGCAAt                                                                 <  2:1503732/150‑1 (MQ=255)
                                                                                        gAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCt                                                               >  1:470298/1‑150 (MQ=255)
                                                                                           aaaaTGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTAc                                                            <  2:557738/150‑1 (MQ=255)
                                                                                             aaTGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGc                                                          <  2:902317/150‑1 (MQ=255)
                                                                                                gatgTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTg                                                       <  2:441810/150‑1 (MQ=255)
                                                                                                  tgTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGtc                                                     >  2:1006812/1‑150 (MQ=255)
                                                                                                      aaTCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGtcatca                                                 >  1:601338/1‑150 (MQ=255)
                                                                                                       aTCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAg                                                <  2:1216204/150‑1 (MQ=255)
                                                                                                       aTCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAg                                                <  1:267403/150‑1 (MQ=255)
                                                                                                        tCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGt                                               <  1:133607/150‑1 (MQ=255)
                                                                                                         cAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCAGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGtc                                              >  1:629271/1‑150 (MQ=255)
                                                                                                         cAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCAGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGtc                                              <  1:1499262/150‑1 (MQ=255)
                                                                                                              tGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATg                                         <  1:446356/150‑1 (MQ=255)
                                                                                                              tGCTGAAAACGTGCGCGGTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATg                                         <  2:635976/150‑1 (MQ=255)
                                                                                                              catgaaCAACGCGCACGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATg                                         >  1:1522677/6‑150 (MQ=255)
                                                                                                                cttaCAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTTGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGcc                                       <  2:1548213/147‑1 (MQ=255)
                                                                                                                   aCAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCAt                                    >  1:1071739/1‑150 (MQ=255)
                                                                                                                       cGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAAcc                                >  2:637172/1‑150 (MQ=255)
                                                                                                                       cGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAAcc                                >  2:1415043/1‑150 (MQ=255)
                                                                                                                       cGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAAcc                                <  2:1324129/150‑1 (MQ=255)
                                                                                                                          gcgcGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTa                             >  1:920782/1‑150 (MQ=255)
                                                                                                                           cgagttgttCATTCCGGATGCTGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTAt                            <  1:356304/147‑1 (MQ=255)
                                                                                                                                tgttCATGCCGGAGGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTGTTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGAtg                       <  2:714184/150‑1 (MQ=255)
                                                                                                                                    taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAAGTCAATGTATTGCAGTTTAGTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATttggt                   >  1:1240922/4‑150 (MQ=37)
                                                                                                                                    cATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTAtt                           <  2:360535/142‑1 (MQ=255)
                                                                                                                                    cATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTAtt                           >  1:360535/1‑142 (MQ=255)
                                                                                                                                    cATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATgtggt                   <  1:1608088/150‑1 (MQ=255)
                                                                                                                                    cATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATgtggt                   <  1:1601800/150‑1 (MQ=255)
                                                                                                                                     aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATgtggtg                  >  2:1163331/3‑150 (MQ=38)
                                                                                                                                     aTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTTAAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATATTACGCTTGTCATCAGTCTCATGCCCATATCCTTATTGATgtggtg                  >  2:354078/1‑150 (MQ=255)
                                                                                                                                     aTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTAGTGCAGGTTAGTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCTTCAGTCTCATGCCCATAACCTTATTGCTgtggtg                  >  1:1057357/1‑150 (MQ=25)
                                                                                                                                        ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCCGTTTATTGGTAGGCCTTATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATgtggtggtg               <  2:554394/150‑1 (MQ=255)
                                                                                                                                          ggATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCTCATAACCTTATTGATGTGGTGGTggg             <  1:29567/150‑1 (MQ=255)
                                                                                                                                           gATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGt            <  1:1114079/150‑1 (MQ=255)
                                                                                                                                                 gcgTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAATTg      <  2:686650/150‑1 (MQ=255)
                                                                                                                                                  cgTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAATTGa     >  2:935247/1‑150 (MQ=255)
                                                                                                                                                    tGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAATTGAgg   >  2:193644/1‑150 (MQ=255)
                                                                                                                                                    tGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAATTGAgg   <  2:794224/150‑1 (MQ=255)
                                                                                                                                                     gAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAATTGAGGc  >  2:30360/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGCGAGGAATGGTAATGGGAAAAAATGATGTCAATCAGATTGCTGACAACGTGCGCGTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTTATTGGTAGGCCTGATAAGCGCAGCGCATCAGGCAATCTTACGCTTGTCATCAGTCTCATGCCCATAACCTTATTGATGTGGTGGTGGGTAAATTGAGGC  >  NC_000913/4079885‑4080183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: