Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,092,795 T→G 5.6% D10A (GAC→GCC)  frvA ← putative enzyme IIA component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,092,7950TG 5.6% 479.1 / 9.5 158D10A (GAC→GCC) frvAputative enzyme IIA component of PTS
Reads supporting (aligned to +/- strand):  ref base T (88/60);  new base G (7/3);  total (95/63)
Fisher's exact test for biased strand distribution p-value = 7.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 160 total aligned reads displayed.

CACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGA  >  NC_000913/4092646‑4092943
                                                                                                                                                     |                                                                                                                                                    
cacCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGt                                                                                                                                                      >  1:200695/1‑150 (MQ=255)
  ccGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGGTTTTTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATAGCCCTGAATAATCACGTCa                                                                                                                                                    >  2:715742/1‑150 (MQ=255)
    ggAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAAt                                                                                                                                                  >  1:125244/1‑150 (MQ=255)
    ggAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAAt                                                                                                                                                  <  1:1406416/150‑1 (MQ=255)
      aaCCAAATCGAGTTGAGTGCATGTTTTCGAGCAGCAGGAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTACAGAACCGAATAAGCGACATTGACCTGATTATTCAGGTAAATAc                                                                                                                                                >  2:145607/1‑150 (MQ=255)
       aCCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCAAACTGCTTCAGAACGGTAATAGCGCCATTGCCCTGAATATTCAGGTCAATAcc                                                                                                                                               >  2:1571005/1‑149 (MQ=255)
         cAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACACTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGc                                                                                                                                             >  1:927582/1‑150 (MQ=255)
              ccAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCa                                                                                                                                        >  2:1465171/1‑150 (MQ=255)
               cAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTTCCCTGAATATTCAGGTCAATACAGCTTGCAg                                                                                                                                       >  2:1334868/1‑150 (MQ=255)
                   agagTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAag                                                                                                                                   >  2:1681687/1‑150 (MQ=255)
                   agagTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAag                                                                                                                                   <  2:601667/150‑1 (MQ=255)
                         cATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGc                                                                                                                             >  2:1446491/1‑150 (MQ=255)
                         cATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGc                                                                                                                             >  2:902402/1‑150 (MQ=255)
                          aTTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGATTATTCAGTTCAATACATCTTGCAGTAAGAGCTGcc                                                                                                                            >  2:1413650/1‑150 (MQ=255)
                          aTTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGcc                                                                                                                            >  1:459784/1‑150 (MQ=255)
                          aTTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGcc                                                                                                                            >  2:952203/1‑150 (MQ=255)
                          aTTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGcc                                                                                                                            <  2:1138426/150‑1 (MQ=255)
                             ttttCGCGCAGCAGCAGGGTTTGCAGGAACTGGCGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCAtt                                                                                                                         <  1:77963/150‑1 (MQ=255)
                              tttCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTa                                                                                                                        <  1:1361972/150‑1 (MQ=255)
                                tCGCGCAGCAGCAGGGTTTGCAGGAACT‑GTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATc                                                                                                                     <  1:1279895/150‑1 (MQ=255)
                                  gcgcAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATCAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCg                                                                                                                    <  1:1647665/150‑1 (MQ=255)
                                  gcgcAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCg                                                                                                                    <  2:1184858/150‑1 (MQ=255)
                                   cgcAGCAGCAGGGTTTGCAGGACCTGGTGTGAGGCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCgg                                                                                                                   <  2:304883/150‑1 (MQ=255)
                                    gcagcagcaGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGa                                                                                                                  <  2:144299/150‑1 (MQ=255)
                                          gcagGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCAt                                                                                                            <  1:1600581/150‑1 (MQ=255)
                                           cagGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATa                                                                                                           >  1:987218/1‑150 (MQ=255)
                                                   gCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAatatat                                                                                                   >  2:244453/1‑150 (MQ=255)
                                                    cAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATAtt                                                                                                  >  1:1394670/1‑150 (MQ=255)
                                                        aaCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTaa                                                                                              <  2:637753/150‑1 (MQ=255)
                                                          cTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATc                                                                                            >  1:585869/1‑150 (MQ=255)
                                                            ggTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCaa                                                                                          >  2:763172/1‑150 (MQ=255)
                                                            ggTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCaa                                                                                          >  2:705635/1‑150 (MQ=255)
                                                            ggTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCaa                                                                                          >  1:507462/1‑150 (MQ=255)
                                                            ggTGTGAGTCGGTGATAAAACCGTTTTGTAAAGCTATTGTCGCCACCTGCTTCAGAACGGAATAAGCGACATTGCACTTAATATTCAGGTAAATACAGATTGCAGTAATAGCTGCCATTACTCTGACTCCATAAAATGTATTTTAATCat                                                                                          >  1:842181/1‑149 (MQ=255)
                                                                 gAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTa                                                                                     >  2:1523511/1‑150 (MQ=255)
                                                                      ggTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAAtggt                                                                                <  1:394195/150‑1 (MQ=255)
                                                                        tGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAAtggtgg                                                                              <  2:1339625/150‑1 (MQ=255)
                                                                        tGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAAtggtgg                                                                              <  2:1695022/150‑1 (MQ=255)
                                                                         gATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGATGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGa                                                                             >  2:1536733/1‑150 (MQ=255)
                                                                            aaaaCCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGACTATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAATTATATTTTAATCAATAGTATGGGGGGAggg                                                                          >  2:872297/1‑148 (MQ=255)
                                                                             aaaCCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGa                                                                         >  2:1153532/1‑150 (MQ=255)
                                                                               aCCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGAtt                                                                       >  1:425416/1‑150 (MQ=255)
                                                                               aCCGTTTTGTAACGCTATTGTCGACAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGAtt                                                                       <  1:387618/150‑1 (MQ=255)
                                                                                  gTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCATGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAg                                                                    <  1:22547/150‑1 (MQ=255)
                                                                                  gTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAg                                                                    <  1:1681687/150‑1 (MQ=255)
                                                                                    tttGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGtg                                                                  <  2:535552/150‑1 (MQ=255)
                                                                                    tttGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGtg                                                                  >  1:1599789/1‑150 (MQ=255)
                                                                                      tGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGtgat                                                                <  1:1536733/150‑1 (MQ=255)
                                                                                       gTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGtgatg                                                               <  1:540965/150‑1 (MQ=255)
                                                                                       gTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGtgatg                                                               >  2:215037/1‑150 (MQ=255)
                                                                                                ttGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGat                                                      <  1:1501384/150‑1 (MQ=255)
                                                                                                 tGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGTAGCGATTAAGTGATGAGAGGCGata                                                     >  1:323487/1‑150 (MQ=255)
                                                                                                 tGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGata                                                     >  1:1049322/1‑150 (MQ=255)
                                                                                                     gCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTTAGGTAAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTc                                                 <  1:1152191/150‑1 (MQ=255)
                                                                                                      ccAACTGCTGCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGCGCGATTATGTGATGCGCGGCGATATGTCg                                                >  2:1233191/1‑150 (MQ=255)
                                                                                                        aaCTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGt                                              >  1:1249089/1‑150 (MQ=255)
                                                                                                            gCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAAc                                          >  2:1027103/1‑150 (MQ=255)
                                                                                                            gCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAAc                                          >  2:1192517/1‑150 (MQ=255)
                                                                                                             cTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACa                                         >  2:1080338/1‑150 (MQ=255)
                                                                                                             cTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACa                                         >  2:1685127/1‑150 (MQ=255)
                                                                                                               tCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAAt                                       >  2:1608224/1‑150 (MQ=255)
                                                                                                               tCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAAt                                       >  2:533083/1‑150 (MQ=255)
                                                                                                                cAGAACGGAATAAGCGCCATTGCCGTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATTGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATg                                      <  1:1376179/150‑1 (MQ=255)
                                                                                                                cAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATg                                      >  2:1495948/1‑150 (MQ=255)
                                                                                                                cAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATg                                      <  2:1278985/150‑1 (MQ=255)
                                                                                                                 aGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGGCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGa                                     <  1:1307987/150‑1 (MQ=255)
                                                                                                                  gAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGAc                                    >  2:619454/1‑150 (MQ=255)
                                                                                                                  caaCGGAATAAGCGCCATTGCCCTGAATAATCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGAc                                    <  1:31960/149‑1 (MQ=255)
                                                                                                                       gAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAAttt                               >  1:468497/1‑150 (MQ=255)
                                                                                                                        aataatCGCCATTGCCCTGAATATTCAGGTCAATAAAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGGCGGTAAACAATGACAATTTg                              <  2:1073195/150‑1 (MQ=255)
                                                                                                                        aaTAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTAAATCAATTGTAATCGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTg                              <  1:902402/150‑1 (MQ=255)
                                                                                                                         aTAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTga                             <  1:1003683/150‑1 (MQ=255)
                                                                                                                         aTAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTga                             >  2:415513/1‑150 (MQ=255)
                                                                                                                            aGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATTTGTCGGTAAACATTGACAATTTGAGAc                          >  2:295384/1‑150 (MQ=255)
                                                                                                                             gcgcCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACa                         >  1:1421472/1‑150 (MQ=255)
                                                                                                                              ctgtaTTGACCTGAATATTCAGGGCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACag                        >  1:1541027/5‑150 (MQ=255)
                                                                                                                              ctgtaTTGACCTGAATATTCAGGGCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACag                        >  1:1574641/5‑150 (MQ=255)
                                                                                                                              cgcCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACag                        >  1:488303/1‑150 (MQ=255)
                                                                                                                                gtaTTGACCTGAATATTCAGGGCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACagag                      >  2:458408/3‑150 (MQ=255)
                                                                                                                                 taTTGACCTGAATATTCAGGGCAATACAGATTGCAGTAAGAGCTGCAATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGt                     >  2:18769/2‑150 (MQ=255)
                                                                                                                                  aTTGACCTGAATATTCAGGGCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTg                    >  1:741834/1‑150 (MQ=255)
                                                                                                                                  aTTGACCTGAAAATTCAGGGCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTg                    >  1:606027/1‑150 (MQ=255)
                                                                                                                                       ccTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAgg               >  1:731077/1‑150 (MQ=255)
                                                                                                                                       ccTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAgg               >  2:1579946/1‑150 (MQ=255)
                                                                                                                                         tGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTc             >  1:146654/1‑150 (MQ=255)
                                                                                                                                          taaTATTCAGCTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCa            <  2:916463/149‑1 (MQ=255)
                                                                                                                                           aaTATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAg           <  2:452990/150‑1 (MQ=255)
                                                                                                                                           aaTATTAAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAg           <  1:1153532/150‑1 (MQ=255)
                                                                                                                                              atTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGAtt        >  2:867468/1‑150 (MQ=255)
                                                                                                                                              atTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTACATAAAATATATATTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGAtt        >  1:255345/1‑150 (MQ=255)
                                                                                                                                               ttCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGAttt       >  1:248726/1‑150 (MQ=255)
                                                                                                                                               ttCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGAttt       >  1:308340/1‑150 (MQ=255)
                                                                                                                                                tCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTg      >  2:1708225/1‑150 (MQ=255)
                                                                                                                                                 cAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACTGAGTGAAAGGTCAGATTTGc     >  2:1159879/1‑150 (MQ=255)
                                                                                                                                                 cAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGGCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGc     <  2:1277402/150‑1 (MQ=255)
                                                                                                                                                 cAGGTAAATACAGCTTGCAGAAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGAAATGGTGGAGCGATTAAGTCAAGAGAGGAGATATGTCGGTAAACAATGACAATTTGAGACAGATTGAAAGGTAAGATTTGc     >  1:832157/1‑150 (MQ=255)
                                                                                                                                                 aaGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGc     <  1:119789/149‑1 (MQ=255)
                                                                                                                                                   ggTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAATGTCAGATTTGCgg   >  2:116372/1‑150 (MQ=255)
                                                                                                                                                   ggTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCgg   >  1:1631489/1‑150 (MQ=255)
                                                                                                                                                    gTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGa  >  1:1504078/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGA  >  NC_000913/4092646‑4092943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑