Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,094,703 C→A 6.0% intergenic (‑431/+20) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,094,7030CA 6.0% 397.2 / 10.2 134intergenic (‑431/+20)rhaD/rhaArhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
Reads supporting (aligned to +/- strand):  ref base C (65/61);  new base A (5/3);  total (70/64)
Fisher's exact test for biased strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

Only 100 of 136 total aligned reads displayed.

CGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACGGCAACGATTTCTGCTCT  >  NC_000913/4094558‑4094852
                                                                                                                                                 |                                                                                                                                                     
cgcgTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCgcgc                                                                                                                                                   <  1:1403614/150‑1 (MQ=40)
 acgTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGACGTAACAGGCCTACCACGGATGACGACCCGTAGGCCGGATAAGGCGCTCgcgca                                                                                                                                                  >  1:683097/2‑149 (MQ=14)
  ggTCTTATCAGGCCTACAACTATTTACGAACTGTAGGCCTGATAAGGCACTTGTGCCGCAGCCGGCATTCAATGCTTGATTCGACGGTGTCGCGTCTTATCAGGCCTACAACTTTTGCCGACCCGTAGGCCGGATAAGGCGCTCGcgccg                                                                                                                                                 <  2:1648210/149‑1 (MQ=11)
  cttCTTATCAGCCCTACAACTATTGCCGACCTGTAGTCCGGATTAGGCACTTGAGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGcgccg                                                                                                                                                 <  1:465142/148‑1 (MQ=255)
  cgTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGcgccg                                                                                                                                                 <  1:670469/150‑1 (MQ=255)
     cTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCAt                                                                                                                                              >  1:1026358/1‑150 (MQ=255)
       tATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCACGCCTACAACAGTTGCCGCCCCGTAGGCCGGATAAGGCGCACGCGCCGCaaac                                                                                                                                            >  1:1104454/1‑147 (MQ=14)
       tATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCAGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATcc                                                                                                                                            >  1:439246/1‑150 (MQ=255)
       tATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTAGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCGTACGACTGTTGCCGACCCGTAGGCCGCATAAGGCGCTCGCGCCGCAGcc                                                                                                                                            >  2:592899/1‑150 (MQ=14)
          cAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATGCGGCGCGAGCGCCTTATCCGGc                                                                                                                                         <  1:757003/150‑1 (MQ=11)
           aGGCCTTCAACTTTTGCCGACCTGTAGGCCGGATAAGTCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATGCGGCGCGAGCGCCTTATCCGGcc                                                                                                                                        <  2:172678/150‑2 (MQ=11)
            ggCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAg                                                                                                                                       <  1:1452647/150‑1 (MQ=255)
            ggCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCAACAACTGTTGCCGACCCGTAGGCCGGATAATGCGCTCGCGCCGCATCCGGCAg                                                                                                                                       >  2:1556534/1‑150 (MQ=255)
              ccTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAgtg                                                                                                                                     >  1:1689232/1‑150 (MQ=255)
               cTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAgtgt                                                                                                                                    >  2:1155560/1‑150 (MQ=255)
                tACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGtt                                                                                                                                   <  2:173675/150‑1 (MQ=255)
                          gCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCgcggc                                                                                                                         >  2:1051279/1‑150 (MQ=255)
                           ccGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCgcggcg                                                                                                                        >  1:1467098/1‑150 (MQ=255)
                               ccTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTc                                                                                                                    >  2:531520/1‑150 (MQ=255)
                                 tGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCaa                                                                                                                  >  1:1373396/1‑150 (MQ=255)
                                  gattgCCGGATGCGGCACAAGTGCCTTATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCaaa                                                                                                                 >  2:1596749/5‑150 (MQ=18)
                                     ggCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAAtt                                                                                                              <  1:686867/150‑1 (MQ=255)
                                      gCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCCTCCGGCAGTGTTTACCCGCGGCGCCTCATAAttt                                                                                                             >  2:840863/1‑150 (MQ=255)
                                      gCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGCCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAAttt                                                                                                             >  2:1398465/1‑150 (MQ=255)
                                       ccGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAAtttc                                                                                                            <  1:82018/150‑1 (MQ=255)
                                       ccGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAAtttc                                                                                                            >  2:422793/1‑150 (MQ=255)
                                        cGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAAtttct                                                                                                           >  2:718472/1‑150 (MQ=255)
                                         ggATAAGGCACTTGTGCCGCATCCGTCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAAtttctt                                                                                                          <  2:1087078/150‑1 (MQ=255)
                                          gATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAAtttcttt                                                                                                         <  1:884230/150‑1 (MQ=255)
                                          gATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCACATCCGGCAGTGTTTACCCGCGGCGACTCAAAAtttcttt                                                                                                         >  2:548808/1‑150 (MQ=255)
                                           aTAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAAtttctttc                                                                                                        <  1:1442885/150‑1 (MQ=255)
                                            tAAGGCCCTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATttctttct                                                                                                       <  2:226092/150‑1 (MQ=255)
                                             aaGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTtctc                                                                                                      <  1:1310879/150‑1 (MQ=255)
                                              aGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCa                                                                                                     <  1:370341/150‑1 (MQ=255)
                                               ggCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCAt                                                                                                    >  2:566601/1‑150 (MQ=255)
                                               ggCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGGTTACCCGCGGCGACTCAAAATTTCGTTCTCaa                                                                                                    >  1:1088113/1‑149 (MQ=255)
                                                gCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATa                                                                                                   >  2:352629/1‑150 (MQ=255)
                                                gCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATa                                                                                                   >  1:238376/1‑150 (MQ=255)
                                                 cACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGCCTCAAAATTTCTTTCTCATaa                                                                                                  >  1:629644/1‑150 (MQ=255)
                                                 cACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATaa                                                                                                  >  2:1057559/1‑150 (MQ=255)
                                                  aCTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAg                                                                                                 <  1:1167936/150‑1 (MQ=255)
                                                     tgtgCCGCATCAGGCAATAAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTCCCGACCCGTAGGCCGGATAAGTCGCTCGCGCCGCATCCGGCAGTGTTTACCCGAGGCGACTCAAAATTTCTTTCTCATAAcccc                                                                                              >  2:1732874/1‑150 (MQ=21)
                                                        gCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCaaa                                                                                                                 >  2:1240232/1‑128 (MQ=255)
                                                        gCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCaaa                                                                                                                 <  1:1240232/128‑1 (MQ=255)
                                                        gCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGcc                                                                                           >  1:749382/1‑149 (MQ=255)
                                                          cGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACg                                                                                         <  2:915884/150‑1 (MQ=255)
                                                          cGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACg                                                                                         <  1:318997/150‑1 (MQ=255)
                                                          cGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGGCTTTTCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACg                                                                                         <  1:719958/150‑1 (MQ=255)
                                                                cGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTcc                                                                                   >  2:1004645/1‑150 (MQ=255)
                                                                cGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCACAAGCCCGCACGCTCTcc                                                                                   <  2:1543628/150‑1 (MQ=255)
                                                                     atcaatGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCCCGCGCTCCAGccc                                                                              >  1:739021/1‑149 (MQ=255)
                                                                       caatGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCagt                                                                            >  1:1232498/1‑148 (MQ=255)
                                                                       caatGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCAACCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCCtt                                                                            >  2:727738/1‑150 (MQ=255)
                                                                        aatGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTc                                                                           >  2:1184582/1‑150 (MQ=255)
                                                                         atGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCg                                                                          <  1:353158/150‑1 (MQ=255)
                                                                         atGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCg                                                                          <  2:439246/150‑1 (MQ=255)
                                                                         atGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCg                                                                          <  1:1183028/150‑1 (MQ=255)
                                                                            ccTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCAACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTa                                                                       >  2:1053258/1‑150 (MQ=255)
                                                                             cTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTAc                                                                      >  2:72037/1‑150 (MQ=255)
                                                                               gATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACct                                                                    >  2:1703152/1‑150 (MQ=255)
                                                                                aTGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACctg                                                                   <  2:223747/150‑1 (MQ=255)
                                                                                             gcgTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTg                                                      <  2:1653767/150‑1 (MQ=255)
                                                                                              cgTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGa                                                     >  2:1404884/1‑150 (MQ=255)
                                                                                               gTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTATTTCTCATAAGCCCGCACGCTCTCAAGCCATTCGCTACCTGCTGGCGTCTCGTGAc                                                    >  2:251896/1‑150 (MQ=255)
                                                                                                    aTCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTgg                                               <  2:1026358/150‑1 (MQ=255)
                                                                                                     tCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCTGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGc                                              <  2:1501172/150‑1 (MQ=255)
                                                                                                     tCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGc                                              <  1:1057559/150‑1 (MQ=255)
                                                                                                        ggCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAAt                                           <  1:929212/150‑1 (MQ=255)
                                                                                                         gCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATa                                          <  1:782829/150‑1 (MQ=255)
                                                                                                         gCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATa                                          <  1:662749/150‑1 (MQ=255)
                                                                                                         gCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCAGCACGCTATACAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATa                                          >  2:1592547/1‑150 (MQ=255)
                                                                                                         gCCTACAACTGTTGCCGAACCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATa                                          <  1:1662512/150‑1 (MQ=255)
                                                                                                          ccTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATAc                                         <  1:50362/150‑1 (MQ=255)
                                                                                                          ccTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCACATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATAc                                         <  2:669000/150‑1 (MQ=255)
                                                                                                           cTACAACTGTTGCCGACCCGTAGGCCGGATTAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGCCGTTGGCAATACa                                        >  2:1620478/1‑150 (MQ=255)
                                                                                                             aCAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACAtt                                      <  1:191026/150‑1 (MQ=255)
                                                                                                                    ttGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGa                               <  1:1556534/150‑1 (MQ=255)
                                                                                                                      gCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTccc                                  >  1:647591/1‑145 (MQ=255)
                                                                                                                      gCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTccc                                  <  2:647591/145‑1 (MQ=255)
                                                                                                                        cGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGc                           >  2:930467/1‑150 (MQ=255)
                                                                                                                          aCCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCt                         >  2:1089961/1‑150 (MQ=255)
                                                                                                                           cccGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTg                        <  2:749382/150‑1 (MQ=255)
                                                                                                                            ccGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGc                       >  2:1700175/1‑150 (MQ=255)
                                                                                                                               actgCCGGATGCGGCGCGAGCGCCTTATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGcccc                    >  2:1584280/4‑148 (MQ=255)
                                                                                                                               actgCCGGATGCGGCGCGAGCGCCTTATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCAc                    >  1:1213730/4‑150 (MQ=255)
                                                                                                                                 ggCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACgg                  <  1:778054/150‑1 (MQ=255)
                                                                                                                                 ggCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACgg                  >  1:1215972/1‑150 (MQ=255)
                                                                                                                                 ggCCGGATAAGGCGCTCGCGCAGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCGGGCGTATCGTGCCGTTGGCAAGACATTTCCCAGCCCGCCTGCCACgg                  >  1:334212/1‑150 (MQ=255)
                                                                                                                                  gCCGGATGCGGCGCGAGCGCCTTATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACGGc                 >  1:535870/1‑150 (MQ=255)
                                                                                                                                  gCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACGGc                 <  2:621459/150‑1 (MQ=255)
                                                                                                                                  gCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACGGc                 >  1:221508/1‑150 (MQ=255)
                                                                                                                                    cGGATGCGGCGCGAGCGCCTTATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACGGCaa               >  1:690538/1‑150 (MQ=255)
                                                                                                                                     ggatgcggCGCGAGCGCCTTATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTAATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACGGCAAc              >  1:1271147/7‑150 (MQ=255)
                                                                                                                                     ggATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACGGCAAc              <  1:627190/150‑1 (MQ=255)
                                                                                                                                      gATAAGGCGCTCGCGCAGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACGGCAACg             <  2:683097/150‑1 (MQ=255)
                                                                                                                                           ggCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACGGCAACGATTTc        <  2:750152/150‑1 (MQ=255)
                                                                                                                                            gcgcTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACGGCAACGATTTCt       <  2:1183483/150‑1 (MQ=255)
                                                                                                                                             cgcTCGCGCCGCATCCGGCAGTGTTTACCCGCGGTGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACGGCAACGATTTCTg      <  1:982690/150‑1 (MQ=255)
                                                                                                                                             cgcTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGCCTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCGGGCGTATCGTGACGTTGGCGATACATTTCCCAGACCGCCTGCCACGGCAACGATTTCTg      >  1:806254/1‑150 (MQ=255)
                                                                                                                                                tCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACGGCAACGATTTCTGctc   <  1:72037/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
CGCGTCTTATCAGGCCTACAACTATTGCCGACCTGTAGGCCGGATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACGGCAACGATTTCTGCTCT  >  NC_000913/4094558‑4094852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: