Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,110,717 A→T 10.0% intergenic (+32/+23) cdh → / ← tpiA CDP‑diacylglycerol phosphotidylhydrolase/triosephosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,110,7170AT10.0% 242.4 / 14.8 98intergenic (+32/+23)cdh/tpiACDP‑diacylglycerol phosphotidylhydrolase/triosephosphate isomerase
Reads supporting (aligned to +/- strand):  ref base A (48/40);  new base T (6/4);  total (54/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.57e-01

Only 100 of 103 total aligned reads displayed.

GATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCG  >  NC_000913/4110568‑4110866
                                                                                                                                                     |                                                                                                                                                     
gATGGTACGGCAGAGTGATAACTCATTTGTATTGCTCGCGACACCACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGata                                                                                                                                                       <  2:1101287/150‑1 (MQ=255)
 aTGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGatat                                                                                                                                                      >  1:755887/1‑150 (MQ=255)
 aTGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGatat                                                                                                                                                      <  2:1260530/150‑1 (MQ=255)
  tGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATg                                                                                                                                                     <  1:1252453/150‑1 (MQ=255)
    ttACGGCAGAGTGATAACTCATTTGTATTTCTGGCGACACAACGATATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCCTCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAATTAAGTGCCGGATATTaa                                                                                                                                                   >  1:1498513/2‑150 (MQ=255)
    gTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGaa                                                                                                                                                   <  1:1359387/150‑1 (MQ=255)
       cGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATc                                                                                                                                                <  1:1141990/150‑1 (MQ=255)
        ggCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATTTCATATcc                                                                                                                                               <  1:188570/150‑1 (MQ=255)
         gCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCAACTGACGCTTAACCGTGCTTCAGCCGAGGAACTTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTCAGGAAAGTAAGTGCCGGATATGAAATCCg                                                                                                                                              <  2:634224/150‑1 (MQ=255)
          cAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCgg                                                                                                                                             <  1:413718/150‑1 (MQ=255)
           agagTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGc                                                                                                                                            <  2:1718479/150‑1 (MQ=255)
           agagTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGc                                                                                                                                            <  2:1644102/150‑1 (MQ=255)
             agTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTTAGATTTTTCTATAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATTAAATCCGGCAc                                                                                                                                          >  2:1041207/1‑150 (MQ=255)
             agTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATTTCATATCCGGCAc                                                                                                                                          <  2:343029/150‑1 (MQ=255)
              gTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATTTCATATCCGGCAct                                                                                                                                         <  1:672345/150‑2 (MQ=255)
               tGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCt                                                                                                                                        >  2:1395452/1‑150 (MQ=255)
                gATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTg                                                                                                                                       <  2:1019632/150‑1 (MQ=255)
                    aCTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAg                                                                                                                                   <  2:1534700/150‑1 (MQ=255)
                     cTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTTCTTCAGCCGAGGAAATTAAGGATCATCAGTGTGACATTTTGCGTTAAGGCTAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCATGCACCTGTCaca                                                                                                                                  >  1:1242411/1‑148 (MQ=255)
                     cTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGa                                                                                                                                  >  2:1260397/1‑150 (MQ=255)
                      tCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGTAAAGTAAGTTCCGGATATGAAATCCGGCACCTGTCAGAc                                                                                                                                 >  1:765287/1‑150 (MQ=255)
                       cATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACt                                                                                                                                <  1:510432/150‑1 (MQ=255)
                           tGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAg                                                                                                                            <  2:1148043/150‑1 (MQ=255)
                           tGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAg                                                                                                                            >  2:547850/1‑150 (MQ=255)
                             tATTGCTGGCGACACAACGAAATCTTCTGACGCTTACCCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGcc                                                                                                                          <  2:881388/150‑1 (MQ=255)
                             tATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGATGAGTTCAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGcc                                                                                                                          >  1:1286380/1‑150 (MQ=255)
                              aTTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGccg                                                                                                                         >  2:521227/1‑149 (MQ=255)
                                  cTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGttt                                                                                                                     >  2:992477/1‑150 (MQ=255)
                                   tGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTa                                                                                                                    <  2:405606/150‑1 (MQ=255)
                                       gACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCg                                                                                                                >  1:685775/1‑150 (MQ=255)
                                        acacAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGc                                                                                                               >  2:703440/1‑150 (MQ=255)
                                         cacaACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCt                                                                                                              >  2:421013/1‑150 (MQ=255)
                                           caACGAAATCTACTGAAGCTTAACCGTTCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAATTGCCGGATATGAAATCCTGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTc                                                                                                            >  2:926621/1‑150 (MQ=255)
                                            aaCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCt                                                                                                           >  2:153406/1‑150 (MQ=255)
                                            aaCGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCt                                                                                                           >  1:1276422/1‑150 (MQ=255)
                                              cGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGTCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGc                                                                                                         <  2:1723311/150‑1 (MQ=255)
                                                aaaTCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAg                                                                                                       <  1:1155834/150‑1 (MQ=255)
                                                  aTCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCt                                                                                                     <  2:906879/150‑1 (MQ=255)
                                                    cTACTGCCGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCttt                                                                                                   >  2:1192442/1‑150 (MQ=255)
                                                       cTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAAc                                                                                                >  1:816514/1‑150 (MQ=255)
                                                       cTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAAc                                                                                                <  2:1676915/150‑1 (MQ=255)
                                                       cTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAAc                                                                                                >  2:567293/1‑150 (MQ=255)
                                                        tGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACg                                                                                               <  2:1722093/150‑1 (MQ=255)
                                                         gACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGa                                                                                              >  2:1704377/1‑150 (MQ=255)
                                                           cGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGAtt                                                                                            >  2:1666700/1‑150 (MQ=255)
                                                           cGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGAtt                                                                                            <  1:1411656/150‑1 (MQ=255)
                                                                      gCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGc                                                                                 >  1:503187/1‑150 (MQ=255)
                                                                         tCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTc                                                                              >  2:1724531/1‑150 (MQ=255)
                                                                          cAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCa                                                                             >  2:442102/1‑150 (MQ=255)
                                                                           aGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAg                                                                            >  2:791634/1‑150 (MQ=255)
                                                                            gCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCCCCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGAAGCTTTAACGATAACTGCGAAGGCGTCAGc                                                                           >  2:887948/1‑150 (MQ=255)
                                                                            gCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGc                                                                           >  1:525094/1‑150 (MQ=255)
                                                                                atgAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTc                                                                       <  2:346839/148‑1 (MQ=255)
                                                                                aGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTc                                                                       <  1:1666700/150‑1 (MQ=255)
                                                                                aGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTc                                                                       <  1:1260397/150‑1 (MQ=255)
                                                                                       tCAGGATCATCAGTGTGAGATTTTGCGTTAAGGAGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAg                                                                <  2:1143200/150‑1 (MQ=255)
                                                                                        cAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTATCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGc                                                               >  2:634257/1‑150 (MQ=255)
                                                                                        cAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGc                                                               <  1:1206257/150‑1 (MQ=255)
                                                                                         aGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGca                                                              >  1:526179/1‑150 (MQ=255)
                                                                                          ggATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGcac                                                             >  1:891082/1‑150 (MQ=255)
                                                                                          ggATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGcac                                                             >  1:1397470/1‑150 (MQ=255)
                                                                                           gATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGcacc                                                            <  2:816514/150‑1 (MQ=255)
                                                                                            atcatcAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGcacca                                                           <  1:968938/150‑1 (MQ=255)
                                                                                             tcatcaGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAATGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGcaccac                                                          <  2:314570/150‑1 (MQ=255)
                                                                                               atcaGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCAccacca                                                        >  2:384096/1‑150 (MQ=255)
                                                                                                  aGTGTGAGATGTTGCGGTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATTAAATCCGGCACCTGTCAGACTTAAGACTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGGCAGCTTTCAGAGAAGCACCaccaacc                                                     <  2:1132527/150‑1 (MQ=255)
                                                                                                     gtgAGATTTTGCGTTAAGGCGAAGAGTTAATGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACATAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAgc                                                  >  2:696790/1‑150 (MQ=255)
                                                                                                      tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAgcg                                                 <  1:496176/150‑1 (MQ=255)
                                                                                                      tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAgcg                                                 <  2:1607358/150‑1 (MQ=255)
                                                                                                      tgAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAgcg                                                 >  1:331083/1‑150 (MQ=255)
                                                                                                           ttttGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCg                                            >  1:90926/1‑150 (MQ=255)
                                                                                                             ttGCGTTAAGGCGAATAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGGCAGCTTTCAGAGAAGCAACACCAACCATCTCGCCGTc                                          <  2:1498513/150‑1 (MQ=255)
                                                                                                              tGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCg                                         >  1:356861/1‑150 (MQ=255)
                                                                                                                cGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGat                                       <  1:1689918/150‑1 (MQ=255)
                                                                                                                cGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGat                                       <  2:755887/150‑1 (MQ=255)
                                                                                                                cGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGat                                       >  1:445099/1‑150 (MQ=255)
                                                                                                                     aGGCGAAGAGTTAAGGAAAGTAAGTGCAGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGAACCACCAACCAGCGCGCCGTCGATATCCg                                  >  1:591282/1‑150 (MQ=255)
                                                                                                                      ggCGAACAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGAAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCgg                                 >  2:14037/1‑150 (MQ=255)
                                                                                                                       gCGAAGAGTTAAGGAAAGTATGTGCCGGATTTGAAATCCGGCACGTGTCAGAGTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAATGCGTCAGCTTTCAGAGAAGCACCACCAACCTGCGCGCCGTCGATATCCGGc                                <  2:1242411/150‑1 (MQ=255)
                                                                                                                       gCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGc                                <  1:1114800/150‑1 (MQ=255)
                                                                                                                           agagTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAg                            <  2:685775/150‑1 (MQ=255)
                                                                                                                            gagTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGc                           >  1:1682926/1‑150 (MQ=255)
                                                                                                                             agTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCa                          >  1:1088448/1‑150 (MQ=255)
                                                                                                                                tAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGAAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCATAGACGCACCACCACCCAGCGCGCCGTCGATATCAGGCTGAGCAAAc                       >  2:1376617/1‑150 (MQ=255)
                                                                                                                                  aGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGCCTTAAGCCTGTTTAGCCGCTTTTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAg                     >  1:160500/1‑150 (MQ=255)
                                                                                                                                   ggAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAATGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGAAAACAGt                    >  2:889654/1‑150 (MQ=255)
                                                                                                                                       agtaagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTg                <  2:525094/150‑1 (MQ=255)
                                                                                                                                         taagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCa              <  2:765287/150‑1 (MQ=255)
                                                                                                                                         taagtGCCGGATATGAAATCCGGCAACTGTCAGACTTCAGCCTGTTTAGCCGCTTCTGAAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGATAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCa              >  1:1691289/1‑150 (MQ=255)
                                                                                                                                         caggtGCCGGATTTCATATCCGGCACTTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCa              >  2:1449228/4‑150 (MQ=255)
                                                                                                                                          aagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAg             <  1:567293/150‑1 (MQ=255)
                                                                                                                                           ggtGCCGGATTTCATATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGc            >  2:688156/2‑150 (MQ=255)
                                                                                                                                           ggtGCCGGATTTCATATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGc            >  2:1444088/2‑150 (MQ=255)
                                                                                                                                           ggtGCCGGATTTCATATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGc            >  2:1492918/2‑150 (MQ=255)
                                                                                                                                            gtGCCGGATTTCATATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAACCAGTTCTGCAGCg           >  1:888377/1‑150 (MQ=255)
                                                                                                                                            gtGCCGGATTTCATATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCg           >  1:1490944/1‑150 (MQ=255)
                                                                                                                                             tGCCGGATATGAAATCCGGCACCTGTCAGAGTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGGCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGt          <  2:1257228/150‑1 (MQ=255)
                                                                                                                                              gCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGtt         >  2:536297/1‑150 (MQ=255)
                                                                                                                                                     atGAAATCCTGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGAcgcg  <  2:150396/150‑1 (MQ=255)
                                                                                                                                                     atGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGAcgcg  >  2:1299402/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATGGTACGGCAGAGTGATAACTCATTTGTATTGCTGGCGACACAACGAAATCTACTGACGCTTAACCGTGCTTCAGCCGAGGAAATTCAGGATCATCAGTGTGAGATTTTGCGTTAAGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGCCTGTTTAGCCGCTTCTGCAGCTTTAACGATTACTGCGAAGGCGTCAGCTTTCAGAGAAGCACCACCAACCAGCGCGCCGTCGATATCCGGCTGAGCAAACAGTTCTGCAGCGTTAGACGCG  >  NC_000913/4110568‑4110866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: