BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,113,473	T→C	6.1%	L61L (TTG→CTG) ‡	uspD →	stress‑induced protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,113,473	0	T	C	6.1%	344.9 / 9.0	116	L61L (TTG→CTG) ‡	uspD	stress‑induced protein
Reads supporting (aligned to +/- strand): ref base T (52/56); new base C (3/4); total (55/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

Only 100 of 125 total aligned reads displayed.

CCGGGAATGAAGAAGATGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGCAGTGCGACCTCCTTGTCTGTGGT  >  NC_000913/4113324‑4113622

cagggAATGAAGAAGATGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTAAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAAt                                                                                                                                                       >  2:1588197/3‑150 (MQ=255)

  gggAATGAAGAAGATGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAAAAGAAGATATTCATCAAttt                                                                                                                                                     >  1:86486/1‑149 (MQ=255)

   ggAATGAAGAAGTTGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAAttgt                                                                                                                                                    <  1:321642/150‑1 (MQ=255)

    gAATGAAGAAGATGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAAttgtt                                                                                                                                                   <  2:1049512/150‑1 (MQ=255)

     aaTGAAGAAGATGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACTCTCACCTGACGTTAATTCATATTGATTATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAAttgttg                                                                                                                                                  <  1:156183/150‑1 (MQ=255)

     aaTGAAGAAGATGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCGGTGTTTCTACTTCCCTGCAACAGTAGATATTCTTCAAtttttg                                                                                                                                                  <  2:767487/150‑1 (MQ=255)

     aaTCAAGAAGATGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGTCGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAAttgttg                                                                                                                                                  <  2:75932/150‑1 (MQ=255)

      aTGAAGAAGATGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTga                                                                                                                                                 >  1:1332986/1‑150 (MQ=255)

        gtagaagaTGCGTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTAACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACTGAAGATATTCTTCAATTGTTgaag                                                                                                                                               <  2:854166/148‑1 (MQ=255)

        gaagaaGATGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTgaag                                                                                                                                               <  1:1201633/150‑1 (MQ=255)

        gaagaaGATGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTgaag                                                                                                                                               <  2:162248/150‑1 (MQ=255)

         aagaagATGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTgaaga                                                                                                                                              <  1:240088/150‑1 (MQ=255)

          agaagaTGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTgaagaa                                                                                                                                             <  2:1557919/150‑1 (MQ=255)

             agaTGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATaa                                                                                                                                          >  1:1226369/1‑150 (MQ=255)

              gaTGGCTTCCTGGTCAATAAAGCCCTGGCGCTCGCCAGACATACTGCCGCTCACCAGACGGTATTTCATCTTGCTGATGGCTTAAGCGAGGTGTATCCGGTTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAg                                                                                                                                         <  2:261575/150‑1 (MQ=255)

              gaTGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAg                                                                                                                                         >  1:1726218/1‑150 (MQ=255)

               aTGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAACAATTGAAGAATatct                                                                                                                                        <  2:1636925/150‑4 (MQ=255)

                tGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGAAGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTc                                                                                                                                       <  2:1387539/150‑1 (MQ=255)

                  ccTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCgg                                                                                                                                     <  1:778267/150‑1 (MQ=255)

                   cTTACTGGTGAATAACGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTAAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAACAATTGAAGAATatcttctg                                                                                                                                    <  1:1490455/150‑8 (MQ=255)

                    ttACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGAt                                                                                                                                   <  1:770627/150‑1 (MQ=255)

                    ttACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGAt                                                                                                                                   <  2:1608738/150‑1 (MQ=255)

                     tACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGATGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATa                                                                                                                                  <  2:654740/150‑1 (MQ=255)

                        tGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACa                                                                                                                               <  2:1702995/150‑1 (MQ=255)

                        tGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACa                                                                                                                               <  1:404027/150‑1 (MQ=255)

                         ggTGAATAAAGCCCTGTAGCTCGCCAGACATAATGACGCTCACCTGAAGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACaa                                                                                                                              <  1:597960/150‑1 (MQ=255)

                         ggTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACaa                                                                                                                              >  1:1538499/1‑150 (MQ=255)

                         ggTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCAACTGACGTTAATTCATATTGATGATGGATTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACaa                                                                                                                              <  1:1095610/150‑1 (MQ=255)

                          gTGAATAAAGCCCTGGAGCTCTCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAg                                                                                                                             <  2:746035/150‑1 (MQ=255)

                           tGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGc                                                                                                                            >  1:900646/1‑150 (MQ=255)

                           tGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGc                                                                                                                            <  2:524767/150‑1 (MQ=255)

                            gAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCt                                                                                                                           >  2:734814/1‑150 (MQ=255)

                            gAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAACAATTGAAGAATa                                                                                                                                           >  2:571305/1‑134 (MQ=255)

                            gAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAACAATTGAAGAATa                                                                                                                                           <  1:571305/134‑1 (MQ=255)

                             aaTAAAGGCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATATCAAGCTg                                                                                                                          <  1:1107322/150‑1 (MQ=255)

                             aaTAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTg                                                                                                                          <  1:930332/150‑1 (MQ=255)

                             aaTAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATAATGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTg                                                                                                                          <  1:63980/150‑1 (MQ=255)

                                aaaGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCTGATAACAAGCTGtat                                                                                                                       >  1:282726/1‑150 (MQ=255)

                                 aaGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGtata                                                                                                                      >  1:405990/1‑150 (MQ=255)

                                   gCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATaaa                                                                                                                    >  2:1064390/1‑150 (MQ=255)

                                     ccgcgAGCTCGCCAGACATAAAGCCGCTCACCTGACGTTAATTCATATTGATGCTGGCTTAAGCGACATGGATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACt                                                                                                                  <  2:1049822/146‑1 (MQ=255)

                                     ccTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACt                                                                                                                  <  1:756511/150‑1 (MQ=255)

                                     ccTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACt                                                                                                                  <  2:685731/150‑1 (MQ=255)

                                     ccTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACt                                                                                                                  <  2:1363028/150‑1 (MQ=255)

                                      cTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACtt                                                                                                                 >  1:102000/1‑149 (MQ=255)

                                        ggAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGAc                                                                                                               <  1:485001/150‑1 (MQ=255)

                                         gAGCTCGCCAGACATAATGACTCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACg                                                                                                              <  1:1170654/150‑1 (MQ=255)

                                              cGCCAGACATAATGCCGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGaaaaa                                                                                                         >  2:1303812/1‑150 (MQ=255)

                                                    aCATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCAGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCa                                                                                                   <  2:1096611/150‑1 (MQ=255)

                                                     cATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCaa                                                                                                  >  1:362195/1‑150 (MQ=255)

                                                        aaTGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATgg                                                                                               >  1:461428/1‑150 (MQ=255)

                                                           gACGCTCACCTGAAGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATACCAAACTGTATAAACTGACGAAAAATATACAATGGCCg                                                                                            >  1:439801/1‑150 (MQ=255)

                                                             cGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGaa                                                                                          >  2:1337644/1‑150 (MQ=255)

                                                                     tGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAAc                                                                                  <  2:1682099/150‑1 (MQ=255)

                                                                        cGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGc                                                                               >  1:1202397/1‑150 (MQ=255)

                                                                                cATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGaa                                                                       >  1:1374954/1‑150 (MQ=255)

                                                                                cATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGaa                                                                       >  1:1613461/1‑150 (MQ=255)

                                                                                cATATTGATCATGGCTAAACCGAGTTGAATCCGGGTATCAACTTCCCTGCAACAGAAGATATTCTTAAATTGTTGAAGAATAATTAGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGTCCGAAGACAAAACTGCGTATAGaa                                                                       >  2:13933/1‑150 (MQ=255)

                                                                                   atTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAAcgc                                                                    >  2:421652/1‑150 (MQ=255)

                                                                                    ttGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAAcgcg                                                                   >  1:1184964/1‑150 (MQ=255)

                                                                                     tgatgaTGGCTTAAGCGAGTTGTATCCGGGTATCTACTTACCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCgg                                                                  >  1:1032061/1‑150 (MQ=255)

                                                                                      gatgatGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGga                                                                 >  2:241352/1‑150 (MQ=255)

                                                                                       atgatgGCTTAATGGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAATATATTCTTCAATTGTTGAAGAATAAGTCGGATATCAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGgag                                                                <  2:287336/150‑1 (MQ=255)

                                                                                          atgGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGaaa                                                             >  1:1427671/1‑150 (MQ=255)

                                                                                           tgGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAAt                                                            <  1:87814/150‑1 (MQ=255)

                                                                                           tgGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAAt                                                            <  1:987151/150‑1 (MQ=255)

                                                                                             gCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGc                                                          >  1:870712/1‑150 (MQ=255)

                                                                                              cTTAATCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGcc                                                         <  1:1225075/150‑1 (MQ=255)

                                                                                              cTTAAGCGAGTTGTATCCGGGTCTCTACTTCCCTGCCACAGAAGATCTTCTTCAATTGTTGAAGAATAAGTCGTATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGcc                                                         >  1:1047022/1‑150 (MQ=255)

                                                                                              cTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGcc                                                         <  2:1226369/150‑1 (MQ=255)

                                                                                              cTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGcc                                                         <  1:311652/150‑1 (MQ=255)

                                                                                              cTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGcc                                                         >  1:695196/1‑150 (MQ=255)

                                                                                                 aaGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGa                                                      <  2:853537/150‑1 (MQ=255)

                                                                                                 aaGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGa                                                      >  1:536784/1‑150 (MQ=255)

                                                                                                    cGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTCTATAAACTGACGAAAAATATTCAATGGCAGAAGACAAAACTGCGAATTGAACGCGGAGAAATGCCGGAAac                                                   >  1:120874/1‑150 (MQ=255)

                                                                                                       gTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACAct                                                >  2:1316522/1‑150 (MQ=255)

                                                                                                          gTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACActgct                                             >  1:1282643/1‑150 (MQ=255)

                                                                                                              ccGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATg                                                           <  1:1525942/132‑1 (MQ=255)

                                                                                                              ccGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATg                                                           >  2:1525942/1‑132 (MQ=255)

                                                                                                                 ggTATCTTCTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGTCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAAtt                                      >  2:171856/1‑150 (MQ=255)

                                                                                                                 ggTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATTCCGGAAACACTGCTGGAAAtt                                      <  2:102000/150‑1 (MQ=255)

                                                                                                                    aTCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATg                                   >  2:1479557/1‑150 (MQ=255)

                                                                                                                     tCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCgg                                                                  <  2:1483852/118‑1 (MQ=255)

                                                                                                                     tCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCgg                                                                  <  2:1477173/118‑1 (MQ=255)

                                                                                                                     tCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCgg                                                                  >  1:1477173/1‑118 (MQ=255)

                                                                                                                      ctactaCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCa                                 >  2:1036739/1‑150 (MQ=255)

                                                                                                                           tCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAag                            >  2:453458/1‑150 (MQ=255)

                                                                                                                             ccTGCAACAGAAGATATTCATCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAagag                          <  2:175779/150‑1 (MQ=255)

                                                                                                                                        acttatTCTTCAACAATTGAAGAATAAATCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCTGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGCAGTGCGAcaa               >  2:1242212/4‑148 (MQ=255)

                                                                                                                                              tcttcAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGCAGTGCGACCTCCTTGt         >  1:488699/1‑150 (MQ=255)

                                                                                                                                               cttcAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGCAGTGCGACCTCCTTGTc        <  2:230499/150‑1 (MQ=255)

                                                                                                                                               cttcAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGCAGTGCGACCTCCTTGTc        <  1:1478575/150‑1 (MQ=255)

                                                                                                                                               cttcAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGCAGTGCGACCTCCTTGTc        >  1:1647680/1‑150 (MQ=255)

                                                                                                                                               cttcAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGCAGTGCGACCTCCTAGTc        >  2:222309/1‑150 (MQ=255)

                                                                                                                                                 tcAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTCGAAATTATGCAAAAAGAGCAGTGCGACCTCCTTGTCtg      >  1:102549/1‑150 (MQ=255)

                                                                                                                                                  cAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGACGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGCAGTGCGACCTCCTTGTCtgt     <  2:323172/150‑1 (MQ=255)

                                                                                                                                                    aTTGTTTAATAATATGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGCAGTGCGACCTCCTTGTCTGTgg   <  2:323720/150‑1 (MQ=255)

                                                                                                                                                    aTTGTTGAAGAATAAGTCTGATAACAAGCTTTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCTTATTGAACGCGGAGAAATTCCGGAAACACTGCTTGAAATTATGCAAAAAGAGCAGTGCGACCTCCTTGTCTGTgg   <  1:463598/150‑1 (MQ=255)

                                                                                                                                                    aTTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTTCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGCAGTGCGACCTCCTTGTCTGTgg   <  1:429788/150‑1 (MQ=255)

                                                                                                                                                     ttgttgAAGAATAAGTCGGATAACAATCTGTATAAACTGACGAAAAATATTCAATGGCCTAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGCAGTGCGACCTCCTTGTCTGTGGt  <  1:1153697/150‑1 (MQ=255)

CCGGGAATGAAGAAGATGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGCAGTGCGACCTCCTTGTCTGTGGT  >  NC_000913/4113324‑4113622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`