BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,113,474	T→A	6.0%	L61* (TTG→TAG) ‡	uspD →	stress‑induced protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,113,474	0	T	A	6.0%	345.6 / 8.7	116	L61* (TTG→TAG) ‡	uspD	stress‑induced protein
Reads supporting (aligned to +/- strand): ref base T (52/57); new base A (3/4); total (55/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.20e-01

Only 100 of 123 total aligned reads displayed.

GGGAATGAAGAAGATGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGCAGTGCGACCTCCTTGTCTGTGGT  >  NC_000913/4113326‑4113622

gggAATGAAGAAGATGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAAAAGAAGATATTCATCAAttt                                                                                                                                                     >  1:86486/1‑149 (MQ=255)

   aaTGAAGAAGATGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACTCTCACCTGACGTTAATTCATATTGATTATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAAttgttg                                                                                                                                                  <  1:156183/150‑1 (MQ=255)

   aaTGAAGAAGATGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCGGTGTTTCTACTTCCCTGCAACAGTAGATATTCTTCAAtttttg                                                                                                                                                  <  2:767487/150‑1 (MQ=255)

   aaTCAAGAAGATGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGTCGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAAttgttg                                                                                                                                                  <  2:75932/150‑1 (MQ=255)

    aTGAAGAAGATGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTga                                                                                                                                                 >  1:1332986/1‑150 (MQ=255)

      gtagaagaTGCGTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTAACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACTGAAGATATTCTTCAATTGTTgaag                                                                                                                                               <  2:854166/148‑1 (MQ=255)

      gaagaaGATGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTgaat                                                                                                                                               >  2:1335171/1‑149 (MQ=255)

      gaagaaGATGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTgaag                                                                                                                                               <  2:162248/150‑1 (MQ=255)

      gaagaaGATGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTgaag                                                                                                                                               <  1:1201633/150‑1 (MQ=255)

       aagaagATGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTgaaga                                                                                                                                              <  1:240088/150‑1 (MQ=255)

        agaagaTGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTgaagaa                                                                                                                                             <  2:1557919/150‑1 (MQ=255)

            gaTGGCTTCCTGGTCAATAAAGCCCTGGCGCTCGCCAGACATACTGCCGCTCACCAGACGGTATTTCATCTTGCTGATGGCTTAAGCGAGGTGTATCCGGTTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAg                                                                                                                                         <  2:261575/150‑1 (MQ=255)

             aTGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAACAATTGAAGAATatct                                                                                                                                        <  2:1636925/150‑4 (MQ=255)

              tGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGAAGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTc                                                                                                                                       <  2:1387539/150‑1 (MQ=255)

                ccTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCgg                                                                                                                                     <  1:778267/150‑1 (MQ=255)

                ccTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCgg                                                                                                                                     <  1:444095/150‑1 (MQ=255)

                 cTTACTGGTGAATAACGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTAAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAACAATTGAAGAATatcttctg                                                                                                                                    <  1:1490455/150‑8 (MQ=255)

                  ttACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGAt                                                                                                                                   <  1:770627/150‑1 (MQ=255)

                   tACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGATGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATa                                                                                                                                  <  2:654740/150‑1 (MQ=255)

                      tGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACa                                                                                                                               <  2:1702995/150‑1 (MQ=255)

                      tGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACa                                                                                                                               <  1:404027/150‑1 (MQ=255)

                       ggTGAATAAAGCCCTGTAGCTCGCCAGACATAATGACGCTCACCTGAAGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACaa                                                                                                                              <  1:597960/150‑1 (MQ=255)

                       ggTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTTCAACAGAAGATATTCTTCAATTGTTGAAGAAAAAGTCGGATAACaa                                                                                                                              <  1:13589/150‑1 (MQ=255)

                       ggTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACaa                                                                                                                              >  1:1538499/1‑150 (MQ=255)

                        gTGAATAAAGCCCTGGAGCTCTCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAg                                                                                                                             <  2:746035/150‑1 (MQ=255)

                         tGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACatga                                                                                                                            >  1:906668/1‑147 (MQ=255)

                         tGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGc                                                                                                                            <  2:524767/150‑1 (MQ=255)

                         tGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGc                                                                                                                            >  1:900646/1‑150 (MQ=255)

                          gAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCt                                                                                                                           >  2:734814/1‑150 (MQ=255)

                          gAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAACAATTGAAGAATa                                                                                                                                           <  1:571305/134‑1 (MQ=255)

                          gAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAACAATTGAAGAATa                                                                                                                                           >  2:571305/1‑134 (MQ=255)

                           aaTAAAGGCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATATCAAGCTg                                                                                                                          <  1:1107322/150‑1 (MQ=255)

                              aaaGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCTGATAACAAGCTGtat                                                                                                                       >  1:282726/1‑150 (MQ=255)

                               aaGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGtata                                                                                                                      >  1:405990/1‑150 (MQ=255)

                                 gCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATaaa                                                                                                                    >  2:1064390/1‑150 (MQ=255)

                                   ccgcgAGCTCGCCAGACATAAAGCCGCTCACCTGACGTTAATTCATATTGATGCTGGCTTAAGCGACATGGATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACt                                                                                                                  <  2:1049822/146‑1 (MQ=255)

                                   ccTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACt                                                                                                                  <  2:685731/150‑1 (MQ=255)

                                   ccTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACt                                                                                                                  <  1:756511/150‑1 (MQ=255)

                                    cTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACtt                                                                                                                 >  1:102000/1‑149 (MQ=255)

                                      ggAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGAc                                                                                                               <  1:485001/150‑1 (MQ=255)

                                       gAGCTCGCCAGACATAATGACTCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACg                                                                                                              <  1:1170654/150‑1 (MQ=255)

                                            cGCCAGACATAATGCCGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGaaaaa                                                                                                         >  2:1303812/1‑150 (MQ=255)

                                              ccAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCTAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAata                                                                                                       >  2:1122571/1‑150 (MQ=255)

                                                  aCATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCAGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCa                                                                                                   <  2:1096611/150‑1 (MQ=255)

                                                   cATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCaa                                                                                                  >  1:362195/1‑150 (MQ=255)

                                                      aaTGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATgg                                                                                               >  1:461428/1‑150 (MQ=255)

                                                      aaTGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATgg                                                                                               <  2:163094/150‑1 (MQ=255)

                                                         gACGCTCACCTGAAGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATACCAAACTGTATAAACTGACGAAAAATATACAATGGCCg                                                                                            >  1:439801/1‑150 (MQ=255)

                                                           cGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGaa                                                                                          >  2:1337644/1‑150 (MQ=255)

                                                               cACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACa                                                                                      >  2:1042114/1‑150 (MQ=255)

                                                                aCCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACaa                                                                                     <  2:1538499/150‑1 (MQ=255)

                                                                   tGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAAc                                                                                  <  2:1682099/150‑1 (MQ=255)

                                                                      cGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGc                                                                               >  1:1202397/1‑150 (MQ=255)

                                                                              cATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGaa                                                                       >  1:1613461/1‑150 (MQ=255)

                                                                              cATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGaa                                                                       >  1:1374954/1‑150 (MQ=255)

                                                                              cATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGaa                                                                       >  1:490810/1‑150 (MQ=255)

                                                                              cATATTGATCATGGCTAAACCGAGTTGAATCCGGGTATCAACTTCCCTGCAACAGAAGATATTCTTAAATTGTTGAAGAATAATTAGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGTCCGAAGACAAAACTGCGTATAGaa                                                                       >  2:13933/1‑150 (MQ=255)

                                                                                 atTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAAcgc                                                                    >  2:421652/1‑150 (MQ=255)

                                                                                   tgatgaTGGCTTAAGCGAGTTGTATCCGGGTATCTACTTACCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCgg                                                                  >  1:1032061/1‑150 (MQ=255)

                                                                                     atgatgGCTTAATGGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAATATATTCTTCAATTGTTGAAGAATAAGTCGGATATCAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGgag                                                                <  2:287336/150‑1 (MQ=255)

                                                                                      tgatgGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGgaga                                                               <  2:144622/150‑1 (MQ=255)

                                                                                         tgGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAAt                                                            <  1:87814/150‑1 (MQ=255)

                                                                                         tgGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAAt                                                            <  1:987151/150‑1 (MQ=255)

                                                                                          ggCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATg                                                           <  1:1064390/150‑1 (MQ=255)

                                                                                           gCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGc                                                          >  1:870712/1‑150 (MQ=255)

                                                                                            cTTAATCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGcc                                                         <  1:1225075/150‑1 (MQ=255)

                                                                                            cTTAAGCGAGTTGTATCCGGGTCTCTACTTCCCTGCCACAGAAGATCTTCTTCAATTGTTGAAGAATAAGTCGTATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGcc                                                         >  1:1047022/1‑150 (MQ=255)

                                                                                            cTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGcc                                                         >  1:695196/1‑150 (MQ=255)

                                                                                            cTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGcc                                                         <  1:311652/150‑1 (MQ=255)

                                                                                            cTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGcc                                                         >  1:382126/1‑150 (MQ=255)

                                                                                               aaGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGa                                                      >  1:536784/1‑150 (MQ=255)

                                                                                               aaGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGa                                                      <  2:853537/150‑1 (MQ=255)

                                                                                                  cGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAac                                                   >  2:245461/1‑150 (MQ=255)

                                                                                                  cGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTCTATAAACTGACGAAAAATATTCAATGGCAGAAGACAAAACTGCGAATTGAACGCGGAGAAATGCCGGAAac                                                   >  1:120874/1‑150 (MQ=255)

                                                                                                     gTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACAct                                                >  2:1316522/1‑150 (MQ=255)

                                                                                                       tGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACActgc                                              <  1:245461/150‑1 (MQ=255)

                                                                                                        gTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACActgct                                             >  1:1282643/1‑150 (MQ=255)

                                                                                                            ccGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATg                                                           >  2:1525942/1‑132 (MQ=255)

                                                                                                               ggTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATTCCGGAAACACTGCTGGAAAtt                                      <  2:102000/150‑1 (MQ=255)

                                                                                                                   tCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCgg                                                                  >  1:1483852/1‑118 (MQ=255)

                                                                                                                   tCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCgg                                                                  >  1:1477173/1‑118 (MQ=255)

                                                                                                                   tCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCgg                                                                  <  2:1477173/118‑1 (MQ=255)

                                                                                                                   tCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCgg                                                                  <  2:1483852/118‑1 (MQ=255)

                                                                                                                    ctactaCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCa                                 >  2:1036739/1‑150 (MQ=255)

                                                                                                                         tCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAag                            >  2:453458/1‑150 (MQ=255)

                                                                                                                           ccTGCAACAGAAGATATTCATCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAagag                          <  2:175779/150‑1 (MQ=255)

                                                                                                                            cTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGc                         <  2:1726218/150‑1 (MQ=255)

                                                                                                                               cAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGCAGt                      >  1:1184402/1‑150 (MQ=255)

                                                                                                                                      acttatTCTTCAACAATTGAAGAATAAATCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCTGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGCAGTGCGAcaa               >  2:1242212/4‑148 (MQ=255)

                                                                                                                                       tatatTATTCAAATGTTGAATAATAAGTCGTATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCTTTTTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGCAGTGCGAcctc              <  1:1588197/149‑1 (MQ=255)

                                                                                                                                             cttcAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGCAGTGCGACCTCCTTGTc        <  2:230499/150‑1 (MQ=255)

                                                                                                                                             cttcAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGCAGTGCGACCTCCTTGTc        <  1:1478575/150‑1 (MQ=255)

                                                                                                                                             cttcAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGCAGTGCGACCTCCTTGTc        >  1:1647680/1‑150 (MQ=255)

                                                                                                                                             cttcAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGCAGTGCGACCTCCTAGTc        >  2:222309/1‑150 (MQ=255)

                                                                                                                                              ttcAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGCAGTGCGACCTCCTTGTCt       >  1:1212727/1‑150 (MQ=255)

                                                                                                                                               tcAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTCGAAATTATGCAAAAAGAGCAGTGCGACCTCCTTGTCtg      >  1:102549/1‑150 (MQ=255)

                                                                                                                                                cAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGACGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGCAGTGCGACCTCCTTGTCtgt     <  2:323172/150‑1 (MQ=255)

                                                                                                                                                  aTTGTTGAAGAATAAGTCTGATAACAAGCTTTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCTTATTGAACGCGGAGAAATTCCGGAAACACTGCTTGAAATTATGCAAAAAGAGCAGTGCGACCTCCTTGTCTGTgg   <  1:463598/150‑1 (MQ=255)

                                                                                                                                                  aTTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGCAGTGCGACCTCCTTGTCTGTgg   <  2:405990/150‑1 (MQ=255)

                                                                                                                                                   ttgttgAAGAATAAGTCGGATAACAATCTGTATAAACTGACGAAAAATATTCAATGGCCTAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGCAGTGCGACCTCCTTGTCTGTGGt  <  1:1153697/150‑1 (MQ=255)

GGGAATGAAGAAGATGCCTTACTGGTGAATAAAGCCCTGGAGCTCGCCAGACATAATGACGCTCACCTGACGTTAATTCATATTGATGATGGCTTAAGCGAGTTGTATCCGGGTATCTACTTCCCTGCAACAGAAGATATTCTTCAATTGTTGAAGAATAAGTCGGATAACAAGCTGTATAAACTGACGAAAAATATTCAATGGCCGAAGACAAAACTGCGTATTGAACGCGGAGAAATGCCGGAAACACTGCTGGAAATTATGCAAAAAGAGCAGTGCGACCTCCTTGTCTGTGGT  >  NC_000913/4113326‑4113622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`