Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,137,912 A→G 5.9% intergenic (‑255/+20) yijF ← / ← gldA DUF1287 family protein/glycerol dehydrogenase, NAD+ dependent; 1,2‑propanediol:NAD+ oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,137,9120AG 5.9% 348.5 / 9.4 121intergenic (‑255/+20)yijF/gldADUF1287 family protein/glycerol dehydrogenase, NAD+ dependent; 1,2‑propanediol:NAD+ oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (47/65);  new base G (2/5);  total (50/71)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.39e-01

Only 100 of 122 total aligned reads displayed.

GAGTTTCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCC  >  NC_000913/4137764‑4138061
                                                                                                                                                    |                                                                                                                                                     
    ttCAGATTACTGGCACAGAGTGCCGGATGCGTAGTGAACGCCTTATCCGGACTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCgg                                                                                                                                                  <  2:264869/150‑1 (MQ=255)
     tCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGATCGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCggg                                                                                                                                                 <  1:367248/150‑1 (MQ=255)
     tCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCggg                                                                                                                                                 <  1:361954/150‑1 (MQ=255)
     tCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCggg                                                                                                                                                 <  2:1061834/150‑1 (MQ=255)
     tCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCggg                                                                                                                                                 >  2:1273143/1‑150 (MQ=255)
     tCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATAGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCggg                                                                                                                                                 >  2:65447/1‑150 (MQ=255)
      cAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCCGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGa                                                                                                                                                >  1:1311467/1‑150 (MQ=255)
         aTTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGtt                                                                                                                                             <  2:1249231/150‑1 (MQ=255)
           tACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTg                                                                                                                                           <  2:1287694/150‑1 (MQ=255)
            aCTGGCACAGAGTGCCGGATGCGGAGTGAACTCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTgg                                                                                                                                          <  1:1119091/150‑1 (MQ=255)
            aCTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCAGGCCTACAAAAGCACGCAAATTCAATATATTTCAGAGATTGCGTAGGCCTGATAATCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTgg                                                                                                                                          <  2:58931/150‑1 (MQ=255)
             cTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGCTTGTCCGGGAGTTTgaa                                                                                                                                         <  2:1114902/150‑3 (MQ=18)
              tGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAg                                                                                                                                        <  1:44090/150‑1 (MQ=255)
               ggCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGCTTGTCCGGGAGTTTgaacg                                                                                                                                       <  2:1216003/150‑5 (MQ=18)
               ggCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGCTTGTCCGGGAGTTTgaacg                                                                                                                                       <  2:1398758/150‑5 (MQ=18)
               ggCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGCTTGTCCGGGAGTTTgaacg                                                                                                                                       <  1:1666241/150‑5 (MQ=18)
               ggCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGt                                                                                                                                       >  2:1395832/1‑150 (MQ=255)
                  acaGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAgg                                                                                                                                    <  2:1667784/150‑1 (MQ=255)
                   caGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGt                                                                                                                                   >  2:1707123/1‑150 (MQ=255)
                   caGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGt                                                                                                                                   >  1:1591832/1‑150 (MQ=255)
                    agagTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGtt                                                                                                                                  <  2:1114258/150‑1 (MQ=255)
                    agagTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGtt                                                                                                                                  <  2:616761/150‑1 (MQ=255)
                          ccGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTccc                                                                                                                            >  2:1220999/1‑150 (MQ=255)
                            ggATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCAGGAGTTTGGAGTAGGTTATTCCCAc                                                                                                                          <  1:644252/150‑1 (MQ=255)
                             gATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCAct                                                                                                                         <  2:1017680/150‑1 (MQ=255)
                                 cGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTg                                                                                                                     <  1:804017/150‑1 (MQ=255)
                                  gtaGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGGTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGc                                                                                                                    <  2:1138630/148‑1 (MQ=255)
                                        aaCGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGaaa                                                                                                              >  2:1403583/1‑150 (MQ=255)
                                        aaCGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGaaa                                                                                                              <  2:471595/150‑1 (MQ=255)
                                        aaCGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGaaa                                                                                                              >  1:75093/1‑150 (MQ=255)
                                         aCGCCTTATCCGGCCTACAAAAGCAAGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACATGACGGGAGTTTGGAGTATGTTATTAACACTCTTGCTGGAAAc                                                                                                             >  1:1065841/1‑150 (MQ=21)
                                            ccTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTATCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCt                                                                                                          <  1:56934/150‑1 (MQ=255)
                                            ccTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGGGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCt                                                                                                          <  1:900293/150‑1 (MQ=255)
                                             cTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGAATCAGGCAATTTTGCGTTCAAACTCCCGGACAAACAGGGAGTTTTGAGTATGTTATTCCCACTCTTGCAGTAAACGCTg                                                                                                         >  2:31605/1‑150 (MQ=25)
                                              ttATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGa                                                                                                        <  1:1406010/150‑1 (MQ=255)
                                               tATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGAc                                                                                                       >  2:1703644/1‑150 (MQ=255)
                                                    ggCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTAc                                                                                                  >  2:943189/1‑150 (MQ=255)
                                                    ggCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTAc                                                                                                  >  1:1103536/1‑150 (MQ=255)
                                                     gCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACt                                                                                                 <  1:833990/150‑1 (MQ=255)
                                                     gCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACt                                                                                                 <  2:110572/150‑1 (MQ=255)
                                                      ccTACATAAGCAAGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGGGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGttgat                                                                                                <  1:151343/150‑5 (MQ=255)
                                                       cTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTgg                                                                                               <  2:1123810/150‑1 (MQ=255)
                                                         aCAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTc                                                                                             >  2:306310/1‑150 (MQ=255)
                                                          cAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCg                                                                                            <  2:117963/150‑1 (MQ=255)
                                                            aaaGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGc                                                                                          <  1:261584/150‑1 (MQ=255)
                                                              aGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTa                                                                                        >  2:513108/1‑150 (MQ=255)
                                                                  cGCAAATTCAATTTATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGGATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGTGTAGGTTATTCCGACTCTTGCAGGAAACGCTGACCGTAATGGTCGGCTACcag                                                                                    >  2:864682/1‑150 (MQ=38)
                                                                    cAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACcagca                                                                                  >  1:1429722/1‑150 (MQ=255)
                                                                    cAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACcagca                                                                                  >  1:1000085/1‑150 (MQ=255)
                                                                    cAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACcagca                                                                                  >  1:369575/1‑150 (MQ=255)
                                                                     aaaTTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACcagcag                                                                                 <  2:948511/150‑1 (MQ=255)
                                                                     aaaTTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACcagcag                                                                                 >  1:1693210/1‑150 (MQ=255)
                                                                       aTTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCagag                                                                               <  2:431967/150‑1 (MQ=255)
                                                                        ttCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAgc                                                                              <  1:553127/150‑1 (MQ=255)
                                                                            atatatTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAgcggcg                                                                          >  2:1541191/1‑150 (MQ=255)
                                                                            atatatTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAgcggcg                                                                          >  1:1720708/1‑150 (MQ=255)
                                                                                atTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTaaa                                                                      <  2:1162295/150‑1 (MQ=255)
                                                                                        gaTTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTTTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCt                                                              >  1:409275/1‑150 (MQ=255)
                                                                                        gaTTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCt                                                              >  1:55707/1‑150 (MQ=255)
                                                                                          ttGAGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTgg                                                            <  1:15304/150‑1 (MQ=255)
                                                                                           tGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGc                                                           <  2:1608382/150‑1 (MQ=255)
                                                                                             cGTAGGCCTGTTAAGCGTAGCGCTTCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGt                                                         <  1:335025/150‑1 (MQ=255)
                                                                                              gTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTTATCTGGCGTc                                                        >  2:1357736/1‑150 (MQ=255)
                                                                                              gTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTc                                                        <  1:1210669/150‑1 (MQ=255)
                                                                                                   ccTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCAACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTTGCGTCGcccc                                                   >  2:1273350/1‑150 (MQ=255)
                                                                                                    cTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGcgccg                                                  <  2:85711/150‑1 (MQ=255)
                                                                                                     tGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGcgccgc                                                 >  1:1104874/1‑150 (MQ=255)
                                                                                                      gATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCgccgcc                                                <  1:1237878/150‑1 (MQ=255)
                                                                                                      gATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCgccgcc                                                <  2:75093/150‑1 (MQ=255)
                                                                                                      gATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCgccgcc                                                <  2:94936/150‑1 (MQ=255)
                                                                                                      gATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCgccgcc                                                <  1:513108/150‑1 (MQ=255)
                                                                                                           gCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCTTACAAGCCGGGAGTTTGGAGTAGGTTATTCCCTCTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGGCGCGACGCCAgatc                                           <  2:437966/150‑4 (MQ=255)
                                                                                                           gCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGGCGCGACGCCAgatc                                           <  2:1072330/150‑4 (MQ=255)
                                                                                                            cGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGGCGCGACGCCAgatca                                          <  2:930468/150‑5 (MQ=255)
                                                                                                            cGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGCTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGGCGCGACGCCAgatca                                          <  1:65447/150‑5 (MQ=255)
                                                                                                             gaaGCGCATCAGGCAATTTTGCGGTCAAACTCCCGGACCCGCCGGGAGTTTGGAGTAGGGTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGGCGCGACGCCAgatcag                                         <  1:151818/148‑6 (MQ=255)
                                                                                                             gTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCAtg                                         <  2:641316/150‑1 (MQ=255)
                                                                                                              tAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGATAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCAtgt                                        <  2:756268/150‑1 (MQ=255)
                                                                                                              tAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCAtgt                                        <  1:1707123/150‑1 (MQ=255)
                                                                                                              tAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGGCGCGACGCCAgatcagg                                        <  2:1720708/150‑7 (MQ=255)
                                                                                                                gcgcATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCAtgttg                                      <  1:943189/150‑1 (MQ=255)
                                                                                                                        ggCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGt                              >  1:1412627/1‑150 (MQ=255)
                                                                                                                                tGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCAGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCtt                      >  2:1401000/1‑150 (MQ=255)
                                                                                                                                  cGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCt                    >  2:1232603/1‑150 (MQ=255)
                                                                                                                                    ttCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGc                  <  1:674757/150‑1 (MQ=255)
                                                                                                                                    tccAAACTCCCGGCTTGTCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGGGAATGGTTTCACCtgctgc                  >  1:90236/3‑150 (MQ=255)
                                                                                                                                      cAAACTCCCGGCTTGTCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGcac                >  2:253303/1‑150 (MQ=255)
                                                                                                                                      cAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGcac                <  1:775663/150‑1 (MQ=255)
                                                                                                                                       aaaCTGCCGGTCCAGCCGGGAGTTTGGAGTAGGTTATTCCCTCTCTTGCAGGAAACGCTGACCGTTCTGGTCGGCTACCAGCAGAGCGGCGTAAACCTTATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGcaca               <  1:1586636/150‑1 (MQ=255)
                                                                                                                                       aaaCTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGcaca               >  1:454590/1‑150 (MQ=255)
                                                                                                                                        aaCTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACAt              >  2:1018786/1‑150 (MQ=255)
                                                                                                                                          cTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCGCTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGc            >  1:889972/1‑150 (MQ=255)
                                                                                                                                          cTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAACCCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGc            >  2:843057/1‑150 (MQ=255)
                                                                                                                                             ccGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCATACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGc         <  2:332129/150‑1 (MQ=255)
                                                                                                                                              cGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCt        <  1:1273143/150‑1 (MQ=255)
                                                                                                                                                gACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTc      <  2:55707/150‑1 (MQ=255)
                                                                                                                                                 aCAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCt     <  2:369575/150‑1 (MQ=255)
                                                                                                                                                  cAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTTCTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGACAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTg    <  2:1433329/150‑1 (MQ=255)
                                                                                                                                                  cAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCAGGTTGTGAATGGTTTCACCTACTGCACATGCCGCTTCTg    >  1:1453116/1‑150 (MQ=255)
                                                                                                                                                    aGCCGGGAGGTTGGAGTAGGTTACTCCCACTCTCGCAGGAAACGCGGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTGGGGAATGGTTTCACCTTCTGCACATGCCGCTTCTGcc  <  1:1305631/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GAGTTTCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCC  >  NC_000913/4137764‑4138061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: