Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,137,913 G→T 6.1% intergenic (‑256/+19) yijF ← / ← gldA DUF1287 family protein/glycerol dehydrogenase, NAD+ dependent; 1,2‑propanediol:NAD+ oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,137,9130GT 6.1% 354.4 / 7.7 121intergenic (‑256/+19)yijF/gldADUF1287 family protein/glycerol dehydrogenase, NAD+ dependent; 1,2‑propanediol:NAD+ oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (47/66);  new base T (2/5);  total (50/71)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

Only 100 of 123 total aligned reads displayed.

GAGTTTCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCA  >  NC_000913/4137764‑4138062
                                                                                                                                                     |                                                                                                                                                     
    ttCAGATTACTGGCACAGAGTGCCGGATGCGTAGTGAACGCCTTATCCGGACTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCgg                                                                                                                                                   <  2:264869/150‑1 (MQ=255)
     tCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGATCGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCggg                                                                                                                                                  <  1:367248/150‑1 (MQ=255)
     tCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCggg                                                                                                                                                  <  1:361954/150‑1 (MQ=255)
     tCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATAGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCggg                                                                                                                                                  >  2:65447/1‑150 (MQ=255)
         aTTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGtt                                                                                                                                              <  2:1249231/150‑1 (MQ=255)
           tACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTg                                                                                                                                            <  2:1287694/150‑1 (MQ=255)
            aCTGGCACAGAGTGCCGGATGCGGAGTGAACTCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTgg                                                                                                                                           <  1:1119091/150‑1 (MQ=255)
             cTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGCTTGTCCGGGAGTTTgaa                                                                                                                                          <  2:1114902/150‑3 (MQ=18)
              tgtcacaGAGTGCCGGATGCGGAGTGAACGCATTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGTTTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGCTTGTCCGGGAGTTTgaac                                                                                                                                         <  2:1083135/147‑4 (MQ=17)
              tGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAg                                                                                                                                         <  1:44090/150‑1 (MQ=255)
               ggCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGCTTGTCCGGGAGTTTgaacg                                                                                                                                        <  1:1666241/150‑5 (MQ=18)
               ggCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGt                                                                                                                                        <  2:1683276/150‑1 (MQ=255)
               ggCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGt                                                                                                                                        >  2:1395832/1‑150 (MQ=255)
                gCACAGAGTGCCGGATGCGGAGTTAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGAGTAGGCCTGATAAGGGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTa                                                                                                                                       <  2:1302616/150‑1 (MQ=255)
                  acaGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAgg                                                                                                                                     <  2:1667784/150‑1 (MQ=255)
                    agagTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGtt                                                                                                                                   <  2:616761/150‑1 (MQ=255)
                    agagTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGtt                                                                                                                                   <  2:1114258/150‑1 (MQ=255)
                          ccGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTccc                                                                                                                             >  2:1220999/1‑150 (MQ=255)
                             gATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCAct                                                                                                                          <  2:1017680/150‑1 (MQ=255)
                                 cGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTg                                                                                                                      >  2:733087/1‑150 (MQ=255)
                                  gtaGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGGTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGc                                                                                                                     <  2:1138630/148‑1 (MQ=255)
                                     gTGAACGCCTTATCCCGCCTACAAAAGCACGCAAATTCTATATATTGCAGAGTTTGCTTAGGCCTGATAAGCGTTGCGCATCAGGCAATTTTGGGTTCTAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTGATGCAgg                                                                                                                  <  2:391022/150‑1 (MQ=21)
                                        aaCGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGaaa                                                                                                               >  2:1403583/1‑150 (MQ=255)
                                        aaCGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGaaa                                                                                                               <  2:471595/150‑1 (MQ=255)
                                        aaCGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGaaa                                                                                                               >  1:75093/1‑150 (MQ=255)
                                         aCGCCTTATCCGGCCTACAAAAGCAAGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACATGACGGGAGTTTGGAGTATGTTATTAACACTCTTGCTGGAAAc                                                                                                              >  1:1065841/1‑150 (MQ=21)
                                            ccTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTATCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCt                                                                                                           <  1:56934/150‑1 (MQ=255)
                                            ccTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGGGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCt                                                                                                           <  1:900293/150‑1 (MQ=255)
                                              ttATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGa                                                                                                         <  1:1406010/150‑1 (MQ=255)
                                               tATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGTCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGAc                                                                                                        <  1:961453/150‑1 (MQ=255)
                                               tATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGAc                                                                                                        >  2:1703644/1‑150 (MQ=255)
                                                    ggCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTAc                                                                                                   >  2:943189/1‑150 (MQ=255)
                                                    ggCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTAc                                                                                                   >  1:1103536/1‑150 (MQ=255)
                                                     gCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACt                                                                                                  <  1:833990/150‑1 (MQ=255)
                                                     gCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACt                                                                                                  <  2:110572/150‑1 (MQ=255)
                                                      ccTACATAAGCAAGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGGGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGttgat                                                                                                 <  1:151343/150‑5 (MQ=255)
                                                         aCAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTc                                                                                              >  2:306310/1‑150 (MQ=255)
                                                          cAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCg                                                                                             <  2:117963/150‑1 (MQ=255)
                                                            aaaGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGc                                                                                           <  1:261584/150‑1 (MQ=255)
                                                              aGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTa                                                                                         >  2:513108/1‑150 (MQ=255)
                                                               gCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCCATTTTGCGTTCAAACTCCCGGCCAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTAc                                                                                        >  1:833702/1‑150 (MQ=255)
                                                               gCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTAc                                                                                        >  1:382772/1‑150 (MQ=255)
                                                                  cGCAAATTCAATTTATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGGATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGTGTAGGTTATTCCGACTCTTGCAGGAAACGCTGACCGTAATGGTCGGCTACcag                                                                                     >  2:864682/1‑150 (MQ=38)
                                                                    cAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACcagca                                                                                   >  1:1000085/1‑150 (MQ=255)
                                                                    cAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACcagca                                                                                   >  1:1429722/1‑150 (MQ=255)
                                                                     aaaTTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACcagcag                                                                                  >  1:1693210/1‑150 (MQ=255)
                                                                     aaaTTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACcagcag                                                                                  <  2:948511/150‑1 (MQ=255)
                                                                       aTTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCagag                                                                                <  2:431967/150‑1 (MQ=255)
                                                                        ttCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAgc                                                                               <  1:553127/150‑1 (MQ=255)
                                                                              atatTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTa                                                                         <  1:1604940/150‑1 (MQ=255)
                                                                                atTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTaaa                                                                       <  2:1159046/150‑1 (MQ=255)
                                                                                atTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTaaa                                                                       <  2:1162295/150‑1 (MQ=255)
                                                                                        gaTTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTTTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCt                                                               >  1:409275/1‑150 (MQ=255)
                                                                                        gaTTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCt                                                               >  1:55707/1‑150 (MQ=255)
                                                                                          ttGAGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTgg                                                             <  1:15304/150‑1 (MQ=255)
                                                                                           tGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGc                                                            <  2:1608382/150‑1 (MQ=255)
                                                                                             cGTAGGCCTGTTAAGCGTAGCGCTTCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGt                                                          <  1:335025/150‑1 (MQ=255)
                                                                                              gTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTTATCTGGCGTc                                                         >  2:1357736/1‑150 (MQ=255)
                                                                                              gTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTc                                                         <  1:1210669/150‑1 (MQ=255)
                                                                                                  gCCTGATAAGCTTAGCGCATCAGTCAATTTTGATTTAAAACTAAAGGACAAGCAGTGAGTTTTGAGTAGGTTATTAACACTCTTGCAGGAAACGCTGACCGTACTGGTAGTCTACCAGCAGAGCGGCGTAAAACTGATCTGGCGTAgcgc                                                     >  2:1245498/1‑150 (MQ=255)
                                                                                                   ccTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCAACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTTGCGTCGcccc                                                    >  2:1273350/1‑150 (MQ=255)
                                                                                                    cTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGcgccg                                                   <  2:85711/150‑1 (MQ=255)
                                                                                                     tGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGcgccgc                                                  >  1:1104874/1‑150 (MQ=255)
                                                                                                      gATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCgccgcc                                                 <  1:1237878/150‑1 (MQ=255)
                                                                                                      gATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCgccgcc                                                 <  2:94936/150‑1 (MQ=255)
                                                                                                           gCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGGCGCGACGCCAgatc                                            <  2:1072330/150‑4 (MQ=255)
                                                                                                            cGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGGCGCGACGCCAgatca                                           <  2:930468/150‑5 (MQ=255)
                                                                                                            cGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGCTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGGCGCGACGCCAgatca                                           <  1:65447/150‑5 (MQ=255)
                                                                                                             gaaGCGCATCAGGCAATTTTGCGGTCAAACTCCCGGACCCGCCGGGAGTTTGGAGTAGGGTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGGCGCGACGCCAgatcag                                          <  1:151818/148‑6 (MQ=255)
                                                                                                             gTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCAtg                                          <  2:641316/150‑1 (MQ=255)
                                                                                                              tAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGATAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCAtgt                                         <  2:756268/150‑1 (MQ=255)
                                                                                                              tAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCAtgt                                         <  1:1707123/150‑1 (MQ=255)
                                                                                                              tAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGGCGCGACGCCAgatcagg                                         <  2:1720708/150‑7 (MQ=255)
                                                                                                               aGCGCATCAGGCAATTTTGCGTTCAAACTCCCAGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACAGGTCGGCTACCAGCAGAGCTGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCAtgtt                                        >  2:563100/1‑150 (MQ=255)
                                                                                                                gcgcATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCAtgttg                                       <  1:943189/150‑1 (MQ=255)
                                                                                                                  gcATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAACCGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGTCGTCGCGCCGCCAGGCATGTtgtg                                     >  2:281090/1‑150 (MQ=255)
                                                                                                                        ggCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGt                               >  1:51744/1‑150 (MQ=255)
                                                                                                                        ggCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGt                               >  1:1412627/1‑150 (MQ=255)
                                                                                                                         gCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTTTTCCCACTCTTGCAGGAAACGCTGCCCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGtt                              >  1:65691/1‑150 (MQ=255)
                                                                                                                          cAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGttt                             >  1:1731118/1‑150 (MQ=255)
                                                                                                                                tGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCAGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCtt                       >  2:1401000/1‑150 (MQ=255)
                                                                                                                                tGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCAGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCtt                       >  2:1408015/1‑150 (MQ=255)
                                                                                                                                  cGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCt                     >  2:1232603/1‑150 (MQ=255)
                                                                                                                                    ttCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGc                   <  1:674757/150‑1 (MQ=255)
                                                                                                                                      cAAACTCCCGGCTTGTCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGcac                 >  2:253303/1‑150 (MQ=255)
                                                                                                                                      cAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGcac                 <  1:775663/150‑1 (MQ=255)
                                                                                                                                       aaaCTGCCGGTCCAGCCGGGAGTTTGGAGTAGGTTATTCCCTCTCTTGCAGGAAACGCTGACCGTTCTGGTCGGCTACCAGCAGAGCGGCGTAAACCTTATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGcaca                <  1:1586636/150‑1 (MQ=255)
                                                                                                                                       aaaCTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGcaca                >  1:454590/1‑150 (MQ=255)
                                                                                                                                        aaCTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACAt               >  2:1018786/1‑150 (MQ=255)
                                                                                                                                          cTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCGCTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGc             >  1:889972/1‑150 (MQ=255)
                                                                                                                                          cTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAACCCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGc             >  2:843057/1‑150 (MQ=255)
                                                                                                                                             ccGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCATACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGc          <  2:332129/150‑1 (MQ=255)
                                                                                                                                              cGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCt         <  1:1273143/150‑1 (MQ=255)
                                                                                                                                                gACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTc       <  2:55707/150‑1 (MQ=255)
                                                                                                                                                 aCAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCt      <  2:369575/150‑1 (MQ=255)
                                                                                                                                                  cAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTTCTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGACAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTg     <  2:1433329/150‑1 (MQ=255)
                                                                                                                                                  cAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTg     <  1:1395832/150‑1 (MQ=255)
                                                                                                                                                  cAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCAGGTTGTGAATGGTTTCACCTACTGCACATGCCGCTTCTg     >  1:1453116/1‑150 (MQ=255)
                                                                                                                                                    aGCCGGGAGGTTGGAGTAGGTTACTCCCACTCTCGCAGGAAACGCGGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTGGGGAATGGTTTCACCTTCTGCACATGCCGCTTCTGcc   <  1:1305631/150‑1 (MQ=255)
                                                                                                                                                     gCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCca  <  2:713824/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAGTTTCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCA  >  NC_000913/4137764‑4138062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: