Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,138,011 C→A 7.6% G342V (GGC→GTC)  gldA ← glycerol dehydrogenase, NAD+ dependent; 1,2‑propanediol:NAD+ oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,138,0110CA 7.6% 359.8 / 13.3 120G342V (GGC→GTC) gldAglycerol dehydrogenase, NAD+ dependent; 1,2‑propanediol:NAD+ oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (56/54);  new base A (4/6);  total (60/60)
Fisher's exact test for biased strand distribution p-value = 7.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 138 total aligned reads displayed.

GCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATT  >  NC_000913/4137862‑4138160
                                                                                                                                                     |                                                                                                                                                     
gCCTGATAAGCTTAGCGCATCAGTCAATTTTGATTTAAAACTAAAGGACAAGCAGTGAGTTTTGAGTAGGTTATTAACACTCTTGCAGGAAACGCTGACCGTACTGGTAGTCTACCAGCAGAGCGGCGTAAAACTGATCTGGCGTAgcgc                                                                                                                                                       >  2:1245498/1‑150 (MQ=255)
 ccTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCAACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTTGCGTCGcccc                                                                                                                                                      >  2:1273350/1‑150 (MQ=255)
  cTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGcgccg                                                                                                                                                     <  2:85711/150‑1 (MQ=255)
   tGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGcgccgc                                                                                                                                                    >  1:1104874/1‑150 (MQ=255)
    gATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCgccgcc                                                                                                                                                   <  1:513108/150‑1 (MQ=255)
    gATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCgccgcc                                                                                                                                                   <  2:75093/150‑1 (MQ=255)
    gATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCgccgcc                                                                                                                                                   <  1:1237878/150‑1 (MQ=255)
         gCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCTTACAAGCCGGGAGTTTGGAGTAGGTTATTCCCTCTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGGCGCGACGCCAgatc                                                                                                                                              <  2:437966/150‑4 (MQ=255)
         gCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGGCGCGACGCCAgatc                                                                                                                                              <  2:1072330/150‑4 (MQ=255)
          cGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGGCGCGACGCCAgatca                                                                                                                                             <  2:930468/150‑5 (MQ=255)
          cGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGCTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGGCGCGACGCCAgatca                                                                                                                                             <  1:65447/150‑5 (MQ=255)
           gaaGCGCATCAGGCAATTTTGCGGTCAAACTCCCGGACCCGCCGGGAGTTTGGAGTAGGGTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGGCGCGACGCCAgatcag                                                                                                                                            <  1:151818/148‑6 (MQ=255)
            tAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGATAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCAtgt                                                                                                                                           <  2:756268/150‑1 (MQ=255)
            tAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGGCGCGACGCCAgatcagg                                                                                                                                           <  2:1720708/150‑7 (MQ=255)
             aGCGCATCAGGCAATTTTGCGTTCAAACTCCCAGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACAGGTCGGCTACCAGCAGAGCTGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCAtgtt                                                                                                                                          >  2:563100/1‑150 (MQ=255)
                gcATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAACCGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGTCGTCGCGCCGCCAGGCATGTtgtg                                                                                                                                       >  2:281090/1‑150 (MQ=255)
                      ggCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGt                                                                                                                                 >  1:51744/1‑150 (MQ=255)
                      ggCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGt                                                                                                                                 >  1:1412627/1‑150 (MQ=255)
                       gCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTTTTCCCACTCTTGCAGGAAACGCTGCCCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGtt                                                                                                                                >  1:65691/1‑150 (MQ=255)
                        cAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGttt                                                                                                                               >  1:1731118/1‑150 (MQ=255)
                              tGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCAGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCtt                                                                                                                         >  2:1408015/1‑150 (MQ=255)
                                  tccAAACTCCCGGCTTGTCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGGGAATGGTTTCACCtgctgc                                                                                                                     >  1:90236/3‑150 (MQ=255)
                                    cAAACTCCCGGCTTGTCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGcac                                                                                                                   >  2:253303/1‑150 (MQ=255)
                                    cAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGcac                                                                                                                   <  1:775663/150‑1 (MQ=255)
                                     aaaCTGCCGGTCCAGCCGGGAGTTTGGAGTAGGTTATTCCCTCTCTTGCAGGAAACGCTGACCGTTCTGGTCGGCTACCAGCAGAGCGGCGTAAACCTTATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGcaca                                                                                                                  <  1:1586636/150‑1 (MQ=255)
                                     aaaCTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGcaca                                                                                                                  >  1:454590/1‑150 (MQ=255)
                                      aaCTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACAt                                                                                                                 >  2:1018786/1‑150 (MQ=255)
                                        cTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAACCCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGc                                                                                                               >  2:843057/1‑150 (MQ=255)
                                               aCAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCt                                                                                                        <  2:369575/150‑1 (MQ=255)
                                                cAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTg                                                                                                       <  1:1395832/150‑1 (MQ=255)
                                                cAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCAGGTTGTGAATGGTTTCACCTACTGCACATGCCGCTTCTg                                                                                                       >  1:1453116/1‑150 (MQ=255)
                                                  aGCCGGGAGGTTGGAGTAGGTTACTCCCACTCTCGCAGGAAACGCGGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTGGGGAATGGTTTCACCTTCTGCACATGCCGCTTCTGcc                                                                                                     <  1:1305631/150‑1 (MQ=255)
                                                         aGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTc                                                                                              >  2:596637/1‑150 (MQ=255)
                                                          gTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCg                                                                                             >  2:689230/1‑150 (MQ=255)
                                                             tGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCAt                                                                                          <  2:532027/150‑1 (MQ=255)
                                                             tGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCAt                                                                                          >  1:1468459/1‑150 (MQ=255)
                                                             tGGAGTAGGTAATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCAt                                                                                          <  1:1515628/150‑1 (MQ=255)
                                                               gAGTAGGTTATTCCCACTCTTGCAGGAAAAGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCAttt                                                                                        >  1:488626/1‑150 (MQ=255)
                                                                    ggTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGcc                                                                                   <  1:1018786/150‑1 (MQ=255)
                                                                       tATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCggg                                                                                <  2:1220886/150‑1 (MQ=255)
                                                                         ttCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGAc                                                                              <  2:1429722/150‑1 (MQ=255)
                                                                             cACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACAtct                                                                          <  1:733087/150‑1 (MQ=255)
                                                                               ctctTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACAtcttc                                                                        <  1:927514/150‑1 (MQ=255)
                                                                                 ctTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATcttctt                                                                      <  1:253303/150‑1 (MQ=255)
                                                                                  ttGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCttt                                                                     <  2:1126531/150‑1 (MQ=255)
                                                                                   tGCAGGAAACGCTGACCGTACTTGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCCCGCCGCCAGGCATGTTGTGAATGGTATAACCTTCTGCACATGCCGCTTCTGACACAATTCGCATTTTCGACGGGACATCTTCTTTa                                                                    >  2:1383103/1‑150 (MQ=255)
                                                                                   tGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTa                                                                    <  1:1403583/150‑1 (MQ=255)
                                                                                   cgCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTa                                                                    >  1:1208116/2‑150 (MQ=255)
                                                                                    gCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTat                                                                   <  1:653627/150‑2 (MQ=255)
                                                                                     cAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAat                                                                  >  2:659955/1‑150 (MQ=255)
                                                                                     cAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAat                                                                  >  1:976405/1‑150 (MQ=255)
                                                                                      aGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAata                                                                 <  1:1568159/150‑1 (MQ=255)
                                                                                      aGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAata                                                                 <  2:1591832/150‑1 (MQ=255)
                                                                                      aGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCACGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTGTAata                                                                 >  1:174471/1‑150 (MQ=255)
                                                                                       ggAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCGCCATTGGCATTTTCGCCGGGCCATCTTCTTTAatat                                                                >  1:1383594/1‑150 (MQ=255)
                                                                                         aaaCGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATcc                                                              <  2:1000085/150‑1 (MQ=255)
                                                                                                  ccGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCg                                                     <  2:1724431/150‑1 (MQ=255)
                                                                                                  ccGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCg                                                     >  2:348040/1‑150 (MQ=255)
                                                                                                     tACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCgaga                                                  <  1:348658/150‑1 (MQ=255)
                                                                                                     tACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAAGTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCgagc                                                  >  2:864560/1‑149 (MQ=255)
                                                                                                      aCTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTTCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGagag                                                 <  2:1103536/150‑1 (MQ=255)
                                                                                                      aCTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGagag                                                 <  1:281090/150‑1 (MQ=255)
                                                                                                         ggTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGttt                                              >  2:670875/1‑149 (MQ=255)
                                                                                                          gTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTAt                                             >  1:1578857/1‑150 (MQ=255)
                                                                                                          gTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTAt                                             <  2:382772/150‑1 (MQ=255)
                                                                                                           tCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGAGATCTTCTTTAATATCCAGTTGAGCGAGAGTTAtt                                            >  2:621992/1‑150 (MQ=255)
                                                                                                           gcGGCTAGCAGCAGAGCGGCGTACACCTGATCGGGCGTCGCGCCGCCTGGCATGTTGTGATTGGTTTCACCTGTTGGACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTAtt                                            <  1:31605/149‑1 (MQ=255)
                                                                                                             ggCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTgg                                          <  2:488626/150‑1 (MQ=255)
                                                                                                             ggCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGAGCCGCCAGGCATGTTTTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATATTCTTTAATATCCAGTTGAGCGAGAGTTATTgg                                          <  1:1273350/150‑1 (MQ=255)
                                                                                                             ggCGACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTgg                                          <  1:1220999/150‑1 (MQ=255)
                                                                                                              gCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGc                                         >  1:222274/1‑150 (MQ=255)
                                                                                                               cTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCa                                        <  2:1412627/150‑1 (MQ=255)
                                                                                                               cTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATACGCATTTTCGCCGCGACATCTACTTTAATATCCAGTAGCGCGAGACTTATTGGCa                                        >  2:81317/1‑150 (MQ=255)
                                                                                                                 aCCAGCAGCGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCaaa                                      >  1:1164807/1‑150 (MQ=255)
                                                                                                                   cagcagAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAAcc                                    <  2:1578857/150‑1 (MQ=255)
                                                                                                                    agcagAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCt                                   >  2:978934/1‑150 (MQ=255)
                                                                                                                       agagCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTAcc                                <  2:1731118/150‑1 (MQ=255)
                                                                                                                       agagCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCGTTAATATCAAGTTGAGCGAGAGTTATTGGCAAACCTAcc                                >  2:1536953/1‑150 (MQ=255)
                                                                                                                             gcgTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGACACAATTCGCATTTTGGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGAATgg                          >  2:477501/1‑150 (MQ=255)
                                                                                                                              cgTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGc                         <  2:147602/150‑1 (MQ=255)
                                                                                                                               gTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCt                        >  1:811853/1‑150 (MQ=255)
                                                                                                                                    acatgccTGGCGGCGCGACGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCGTTTTCGCCGGGGCATCTTCGTTAATATCCAGTTGAGCGAGAGTTATTGGCACACCTACCGCGTGGCTAAggg                   >  1:467734/7‑150 (MQ=255)
                                                                                                                                     cTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGc                  <  1:469762/150‑1 (MQ=255)
                                                                                                                                     cTGATCTGGCGGCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGc                  <  1:480042/150‑1 (MQ=255)
                                                                                                                                       taTCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGCAg                <  2:313690/149‑1 (MQ=255)
                                                                                                                                       gATCTGGCGTCGCGCCGCCAGGCATGTTGGGAATGGTTTCACCTTCTGCACCTGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGCAg                <  2:1311467/150‑1 (MQ=255)
                                                                                                                                         tCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGCAGCt              >  1:996679/1‑150 (MQ=255)
                                                                                                                                         ccTGGCGGCGCGACGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAAATCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGCAGCt              >  2:294805/2‑150 (MQ=255)
                                                                                                                                            ggcggcGCGACGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGCAGCTACg           >  2:953820/1‑150 (MQ=255)
                                                                                                                                            ggCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGCAGCTACg           <  1:546818/150‑1 (MQ=255)
                                                                                                                                                 cgcgCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTc      >  1:1528257/1‑150 (MQ=255)
                                                                                                                                                 cgcgCCGCCAGGCATGTTGTGAATGGTTTCACATTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTc      >  1:1136755/1‑150 (MQ=255)
                                                                                                                                                  gcgcCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCg     <  2:627763/150‑1 (MQ=255)
                                                                                                                                                  gcgcCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCg     <  2:633823/150‑1 (MQ=255)
                                                                                                                                                  gcgcCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCg     >  2:1602704/1‑150 (MQ=255)
                                                                                                                                                  gcgcCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCg     <  2:90236/150‑1 (MQ=255)
                                                                                                                                                    gccgccAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGCa                 <  2:1386453/136‑1 (MQ=255)
                                                                                                                                                     ccgccAGGCATGTTGTGAATGTTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCGAGTTGAGCGAGAGTTATTTGAAAACCTACCGCATGGCTAAGGGCAGATACGGTTTCGAtt  <  1:1245498/150‑1 (MQ=255)
                                                                                                                                                     ccgccAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGAtt  >  2:460590/1‑150 (MQ=255)
                                                                                                                                                     ccgccAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCAAtt  >  2:1680094/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTGCCACAATTCGCATTTTCGCCGGGACATCTTCTTTAATATCCAGTTGAGCGAGAGTTATTGGCAAACCTACCGCATGGCTAAGGGCAGCTACGGTTTCGATT  >  NC_000913/4137862‑4138160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: