Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,142,509 T→G 5.8% intergenic (‑288/‑21) frwA ← / → frwC putative PTS enzyme: Hpr, enzyme I and II components/putative enzyme IIC component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,142,5090TG 5.8% 471.6 / 10.9 148intergenic (‑288/‑21)frwA/frwCputative PTS enzyme: Hpr, enzyme I and II components/putative enzyme IIC component of PTS
Reads supporting (aligned to +/- strand):  ref base T (77/61);  new base G (3/6);  total (81/67)
Fisher's exact test for biased strand distribution p-value = 3.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 151 total aligned reads displayed.

CGGCATGCTTCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGG  >  NC_000913/4142360‑4142657
                                                                                                                                                     |                                                                                                                                                    
cGGCATGCTTCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGATTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCttt                                                                                                                                                      >  2:1445049/1‑150 (MQ=255)
     tGCTTCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCaa                                                                                                                                                 <  1:683312/150‑1 (MQ=255)
       cTTCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGc                                                                                                                                               >  1:1328387/1‑150 (MQ=255)
            ggTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTGCGAAAGcccatg                                                                                                                                          <  1:433586/150‑4 (MQ=255)
            ggTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTACGCCTGCGATTGCCTATTGTTTCATCCATCAAAAATATGGACATGTGCTTTCGCAAGCCCggg                                                                                                                                          >  1:12922/1‑150 (MQ=255)
              tttATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGag                                                                                                                                        >  2:1061144/1‑150 (MQ=255)
                tATCGCAAGTTATGAGGCGGATAGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATTGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTGCGAAAGcccatgtcca                                                                                                                                      <  2:1649780/150‑8 (MQ=255)
                  tCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGagcagc                                                                                                                                    >  1:1014971/1‑150 (MQ=255)
                  tCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGagcagc                                                                                                                                    <  1:1702233/150‑1 (MQ=255)
                   cGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCt                                                                                                                                   >  2:1352280/1‑150 (MQ=255)
                    gCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCtt                                                                                                                                  >  2:1075080/1‑150 (MQ=255)
                    gCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCtt                                                                                                                                  <  2:453451/150‑1 (MQ=255)
                      aaGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTat                                                                                                                                <  1:439777/150‑1 (MQ=255)
                      aaGTTATGAGCCGGATCGCATTTTAGTACTGATATTACAAAAGTCCAGTAAAAGGACTTTTTATCCACTTTTTGCTCCGCCTGCGATTGCCTATAGTTTCCTCCATCAAAAATATGGACATGGGCTTACGCAAGACCGGCAGCAGCTTat                                                                                                                                >  1:959157/1‑150 (MQ=255)
                       aGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTatg                                                                                                                               <  2:1651521/150‑1 (MQ=255)
                       aGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTatg                                                                                                                               <  1:632477/150‑1 (MQ=255)
                        gTTAGGATGCGGATAGCATTTTTGTACTGATATTACAACAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTatga                                                                                                                              <  2:280606/150‑1 (MQ=255)
                           aTGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGGCTTTTTATCGACTGTTTGCTCAGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCTAGCCCGGGGGCAGCTTatgaatg                                                                                                                           >  1:288885/1‑150 (MQ=255)
                           aTGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTatgaatg                                                                                                                           <  2:1691734/150‑1 (MQ=255)
                             gAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAg                                                                                                                         <  1:1468986/150‑1 (MQ=255)
                             gAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCATTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTatgaatgat                                                                                                                         >  2:1226089/1‑149 (MQ=255)
                                  ggATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTTTTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTggg                                                                                                                    >  2:939744/1‑149 (MQ=255)
                                  ggATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGt                                                                                                                    <  1:1238388/150‑1 (MQ=255)
                                    aTCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTACTCCATCAAAAATATGTACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATTAGTTAGTGc                                                                                                                  >  1:320709/1‑150 (MQ=255)
                                      cGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAg                                                                                                                <  1:1063032/150‑1 (MQ=255)
                                       gCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAATTATGGACATGGGCTTTCGCAAGCCCGGGTGCAGCTTATGAATGAGTTGGTGCAGa                                                                                                               >  1:152083/1‑150 (MQ=255)
                                       gCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGa                                                                                                               >  2:1543612/1‑150 (MQ=255)
                                       gCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGa                                                                                                               <  1:385681/150‑1 (MQ=255)
                                         aTTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATc                                                                                                             <  2:1419879/150‑1 (MQ=255)
                                         aTTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATc                                                                                                             >  1:788303/1‑150 (MQ=255)
                                          tttttGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATcc                                                                                                            <  2:173478/150‑1 (MQ=255)
                                            tttGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTg                                                                                                          >  2:1330623/1‑150 (MQ=255)
                                              tGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGaa                                                                                                        <  1:1525808/150‑1 (MQ=255)
                                              tGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGaa                                                                                                        <  1:2766/150‑1 (MQ=255)
                                                 aCTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGaaaaa                                                                                                     >  1:186534/1‑150 (MQ=255)
                                                 aCTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGaaaaa                                                                                                     >  1:51156/1‑150 (MQ=255)
                                                 aCTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGaaaaa                                                                                                     >  1:887470/1‑150 (MQ=255)
                                                  cTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGATTGAGTTGGTGCTGATCCTGaataat                                                                                                    >  2:1581879/1‑150 (MQ=255)
                                                  cTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAAt                                                                                                    >  2:228117/1‑150 (MQ=255)
                                                  cTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAAt                                                                                                    >  2:1693357/1‑150 (MQ=255)
                                                  cTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAAt                                                                                                    >  2:1161097/1‑150 (MQ=255)
                                                    gATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCATATCCTGAAAAATAc                                                                                                  >  1:1092445/1‑150 (MQ=255)
                                                    gATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATAc                                                                                                  >  1:1077489/1‑150 (MQ=255)
                                                       ggataaaaaGGCCATTTACTGGACTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCg                                                                                               >  1:547464/6‑150 (MQ=255)
                                                        ttACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGt                                                                                              >  1:991326/1‑150 (MQ=255)
                                                        ttACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACACGt                                                                                              >  2:841052/1‑150 (MQ=255)
                                                           cAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAg                                                                                           <  1:678044/150‑1 (MQ=255)
                                                                   cAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAAt                                                                                   >  2:207608/1‑150 (MQ=255)
                                                                   cAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAAt                                                                                   >  2:1095499/1‑150 (MQ=255)
                                                                   cAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCCGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAAt                                                                                   >  1:482810/1‑150 (MQ=255)
                                                                    aGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCAttt                                                                                      >  1:512664/1‑146 (MQ=255)
                                                                    aGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATg                                                                                  >  2:555554/1‑150 (MQ=255)
                                                                    aGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATg                                                                                  >  2:554800/1‑150 (MQ=255)
                                                                     gTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATAcc                                                                                                 >  1:1447791/1‑134 (MQ=255)
                                                                       aaaTTGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACg                                                                               <  1:875693/150‑1 (MQ=255)
                                                                         aTGGCGTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATTGACATGTGCTTTCGAAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACggg                                                                             <  1:556703/150‑1 (MQ=255)
                                                                         aTGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGTCATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACggg                                                                             >  1:1407399/1‑150 (MQ=255)
                                                                           ggCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCg                                                                           >  2:1483742/1‑150 (MQ=255)
                                                                             ccTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGtt                                                                         >  2:899842/1‑150 (MQ=255)
                                                                             ccTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGtt                                                                         >  1:742343/1‑150 (MQ=255)
                                                                             ccTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGtt                                                                         <  2:1561069/150‑1 (MQ=255)
                                                                              cTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGttt                                                                        >  2:1382867/1‑150 (MQ=255)
                                                                              cTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGttt                                                                        <  1:471374/150‑1 (MQ=255)
                                                                                   tATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTcacac                                                                   <  2:186534/150‑1 (MQ=255)
                                                                                     tCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTcacacaa                                                                 >  2:1106604/1‑149 (MQ=255)
                                                                                      ccACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATg                                                                <  1:1352280/150‑1 (MQ=255)
                                                                                      ccACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGCGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATg                                                                >  2:41652/1‑150 (MQ=255)
                                                                                          tGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTc                                                            <  2:780517/150‑1 (MQ=255)
                                                                                          tGTTTGATCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTCTGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTc                                                            <  2:845907/150‑1 (MQ=255)
                                                                                            tttGCTCCGCCTGCGATTGGCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTccc                                                          <  1:1668646/150‑1 (MQ=255)
                                                                                                cTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCtttg                                                      <  1:1645422/150‑3 (MQ=255)
                                                                                                   cGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTgg                                                   <  1:1219853/150‑1 (MQ=255)
                                                                                                      cTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTAt                                                <  2:1391318/150‑1 (MQ=255)
                                                                                                         cGATTGCCTATTGTTTCCTCCATCAAAAATATGGAGATGGGCTTTCGCAAGCCCGGGTGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCgg                                             <  2:320709/150‑1 (MQ=255)
                                                                                                          gATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCggg                                            <  2:600014/150‑1 (MQ=255)
                                                                                                          gATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCggg                                            >  1:1237470/1‑150 (MQ=255)
                                                                                                                  aTTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTAttt                                    <  1:1013386/150‑1 (MQ=255)
                                                                                                                   ttctgtcctccAGCACAAATATGCACATGGGCTTGCTCAAGCCCGGGAGCCGCTTATGCATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGCCGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTAtttt                                   <  2:251373/145‑1 (MQ=255)
                                                                                                                     gTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGc                                 >  1:1185076/1‑150 (MQ=255)
                                                                                                                      tttCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCt                                <  2:887470/150‑1 (MQ=255)
                                                                                                                      tttCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCt                                <  2:51156/150‑1 (MQ=255)
                                                                                                                       ttCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTg                               <  1:1061144/150‑1 (MQ=255)
                                                                                                                         cctccATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTcaca                                                                    <  2:1618115/111‑1 (MQ=255)
                                                                                                                         cctccATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGc                             <  1:1309237/150‑1 (MQ=255)
                                                                                                                          ctccATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCAGTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCg                            >  2:713292/1‑150 (MQ=255)
                                                                                                                             cATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTAGTTTGCTGGCGGtt                         >  2:1278500/1‑150 (MQ=255)
                                                                                                                              aTCAAAAATATGGACATGGGCTTTAGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGAAGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGttt                        >  2:1499298/1‑150 (MQ=255)
                                                                                                                                cAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTcc                      >  1:155847/1‑150 (MQ=255)
                                                                                                                                cAAAAATATGGACATGGGCTTTCCCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTcc                      <  2:1672226/150‑1 (MQ=255)
                                                                                                                                 aaaaaTATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCg                     <  2:578939/150‑1 (MQ=255)
                                                                                                                                   aaaTATGGACATGGGCTTTCGCAAGCCCGTGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATCCCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTAGCGGGCGGTATTTTGCTGGCGGTTTCCGTc                   <  2:1040193/150‑1 (MQ=255)
                                                                                                                                       atGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCAtgt               >  2:31218/1‑150 (MQ=255)
                                                                                                                                        tGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAAAACCCTTCAGCATATAATGACGTGCGTTTCACACATGATTCCCTTCGTGGTATCGTGAGGTATTTTGCTGGCGGTTTCCGTCAggtt              >  2:272280/1‑150 (MQ=255)
                                                                                                                                         gctcccgggCTTGCGAAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCAGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGATTCCGTCAtgttg             >  1:1595735/7‑150 (MQ=255)
                                                                                                                                            cccgggCTTGCGAAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTAt          >  1:1404239/4‑150 (MQ=255)
                                                                                                                                            cATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTAt          >  1:621095/1‑150 (MQ=255)
                                                                                                                                               gggCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCGTTCGTGGTATCGGGCGGTAATTTGCTGGCGGTTTCCGTCATGTTGTATGGc       >  2:1556217/1‑150 (MQ=255)
                                                                                                                                               gggCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGc       <  2:1285125/150‑1 (MQ=255)
                                                                                                                                                  cTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCaaa    >  2:404120/1‑150 (MQ=255)
                                                                                                                                                    ttCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAgg  >  2:1721343/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGGCATGCTTCCGGTTTATCGCAAGTTATGAGGCGGATCGCATTTTTGTACTGATATTACAAAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGCATTTAATGACGGGCGTTTCACACATGATTCCCTTCGTGGTATCGGGCGGTATTTTGCTGGCGGTTTCCGTCATGTTGTATGGCAAAGG  >  NC_000913/4142360‑4142657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: