Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 514,496 G→T 5.1% P122T (CCT→ACT)  ybbJ ← inner membrane protein; stimulator of the QmcA suppressor of ftsH‑htpX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913514,4960GT 5.1% 301.5 / 4.4 100P122T (CCT→ACT) ybbJinner membrane protein; stimulator of the QmcA suppressor of ftsH‑htpX
Reads supporting (aligned to +/- strand):  ref base G (35/58);  new base T (2/3);  total (38/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01

Only 100 of 101 total aligned reads displayed.

GACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATT  >  NC_000913/514347‑514642
                                                                                                                                                     |                                                                                                                                                  
gACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGAGTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAgg                                                                                                                                                    <  1:180419/150‑1 (MQ=255)
 aCTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCTGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGc                                                                                                                                                   <  2:1681524/150‑1 (MQ=255)
 aCTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGc                                                                                                                                                   <  1:644701/150‑1 (MQ=255)
   tGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCa                                                                                                                                                 <  1:145666/150‑1 (MQ=255)
      cggtttttCGATAATCTCTCCGGCTGCTGTCAGCATCGTGCTGGGTGAATAAGACGCGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGa                                                                                                                                              <  1:341012/143‑1 (MQ=255)
        gATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGTTCATggcc                                                                                                                                            <  1:1204616/150‑4 (MQ=255)
          ttATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTg                                                                                                                                          <  2:2207/150‑1 (MQ=255)
            aTCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTc                                                                                                                                        <  2:1678081/150‑1 (MQ=255)
               gAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAATACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGGGTACCTGCTCCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCAcc                                                                                                                                     <  1:726935/150‑1 (MQ=255)
               gAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGTTCATGGCCTGTCagc                                                                                                                                     <  1:494094/150‑3 (MQ=255)
               gAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCAcc                                                                                                                                     >  2:630597/1‑150 (MQ=255)
               gAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCAcc                                                                                                                                     >  1:1370019/1‑150 (MQ=255)
                aaaaTCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCg                                                                                                                                    >  1:47941/1‑150 (MQ=255)
                 aaaTCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCgc                                                                                                                                   >  2:796518/1‑149 (MQ=255)
                 aaaTCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGa                                                                                                                                   <  1:232138/150‑1 (MQ=255)
                  aaTCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGTTCATGGCCTGTCAGCgcc                                                                                                                                  <  1:525199/150‑3 (MQ=255)
                  aaTCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGAc                                                                                                                                  <  1:398680/150‑1 (MQ=255)
                  aaTCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATACTCGCTGGCGCTGACAGGCCATGAACTGTCACCGAc                                                                                                                                  >  2:906092/1‑150 (MQ=255)
                   aTCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGAcg                                                                                                                                 >  1:578787/1‑150 (MQ=255)
                      tctcCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGACCATGAACTGTCACCGACgcgc                                                                                                                              >  1:1094418/1‑150 (MQ=255)
                         ccGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCata                                                                                                                           <  1:561183/150‑1 (MQ=255)
                           ggCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATg                                                                                                                         <  2:376059/150‑1 (MQ=255)
                           ggCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATg                                                                                                                         <  1:662306/150‑1 (MQ=255)
                            gctgctGTCATCTTCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGAGTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGa                                                                                                                        <  2:1173334/150‑1 (MQ=255)
                            gctgctGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGa                                                                                                                        <  2:253601/150‑1 (MQ=255)
                            gctgctGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGa                                                                                                                        >  1:1219449/1‑150 (MQ=255)
                              tgctgTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGAcc                                                                                                                      <  1:1108439/150‑1 (MQ=255)
                              tgctgTCATCATCGGGCAGGGTGAATAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGAcc                                                                                                                      <  2:1068712/150‑1 (MQ=255)
                                 tgTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACcgcg                                                                                                                   >  1:302676/1‑150 (MQ=255)
                                  gTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCgcgc                                                                                                                  >  1:384783/1‑150 (MQ=255)
                                  gTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCACAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCgcgc                                                                                                                  >  1:222571/1‑150 (MQ=255)
                                    catcatCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAAGATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGccc                                                                                                                <  2:43048/150‑1 (MQ=255)
                                    catcatCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGccc                                                                                                                >  1:1336964/1‑150 (MQ=255)
                                     atcatcGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCg                                                                                                               >  1:655191/1‑150 (MQ=255)
                                       catcGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGtt                                                                                                             <  2:1161034/150‑1 (MQ=255)
                                       catcGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGtt                                                                                                             <  1:127747/150‑1 (MQ=255)
                                        atcGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCCCCGACGCGCATATGACCGCGCCCGTTg                                                                                                            >  2:885361/1‑150 (MQ=255)
                                        atagggCAGGGGTATTAAGACGAGCCGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGGACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTg                                                                                                            <  2:1059154/147‑1 (MQ=255)
                                         tCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGA                                                                                                           <  1:1586367‑M1/150‑1 (MQ=255)
                                           gggCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGTAATTACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACC                                                                                                         <  2:307358‑M1/150‑1 (MQ=255)
                                              cAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGC                                                                                                      <  1:1367768‑M1/150‑1 (MQ=255)
                                              cAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGC                                                                                                      <  2:31271‑M1/150‑1 (MQ=255)
                                               aGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCG                                                                                                     <  1:1438226‑M1/150‑1 (MQ=255)
                                               aGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCG                                                                                                     <  2:1547417‑M1/150‑1 (MQ=255)
                                                gggTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCgg                                                                                                    <  2:520133/150‑1 (MQ=255)
                                                  gttaTTAAGACGAGACGGCTCGGATATGTAGGGGAATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCCGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGgag                                                                                                  <  1:881618/147‑1 (MQ=255)
                                                  gTGATTCAGACGAGACCGCTCGGAGATGGAGGGGTATCCCTTCAATCGCCATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGgag                                                                                                  <  1:262892/150‑1 (MQ=255)
                                                    gATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGCCCAGCGgagct                                                                                                >  1:493748/1‑148 (MQ=255)
                                                        aaGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCAATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCa                                                                                            >  1:730436/1‑150 (MQ=255)
                                                         agacgataagGCTCGGATATGTATGGTTATCCCTTCTATCGCAATGACTTCAACATTCGTACCTTCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGAGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAg                                                                                           <  2:341902/150‑1 (MQ=255)
                                                         agacgagacgGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAg                                                                                           >  2:1042852/1‑150 (MQ=255)
                                                         agacgagacgGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAg                                                                                           >  2:376733/1‑150 (MQ=255)
                                                          gacgagacgGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGc                                                                                          <  2:1159746/150‑1 (MQ=255)
                                                             gagacgGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGcaca                                                                                       <  1:312053/150‑1 (MQ=255)
                                                                 cgGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAAc                                                                                   <  2:637523/150‑1 (MQ=255)
                                                                   gCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGt                                                                                 <  1:841105/150‑1 (MQ=255)
                                                                       ggATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGc                                                                             >  2:1447456/1‑150 (MQ=255)
                                                                       ggATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATCAACTGTCACCGACGCGCATATGACCGCCCCCGTAGACCAGCGGAGAATCCAGCACAAAACGTCGGc                                                                             >  1:521980/1‑150 (MQ=255)
                                                                        gATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGcc                                                                            <  2:1373426/150‑1 (MQ=255)
                                                                         ttatGTAGGGTTATCCCTTCTATCGCAATGACATCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCa                                                                           <  2:1130241/149‑1 (MQ=255)
                                                                            tGTAGGGTTATCCATTATATCGCATTGACTTCAACATGCGTAACTGCTCAGAGATCCTCGCTGGCGCTGACTGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATc                                                                        <  2:1522875/150‑1 (MQ=255)
                                                                             gTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCGCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCa                                                                       <  1:338152/150‑1 (MQ=255)
                                                                               aGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAgc                                                                     <  2:1604178/150‑1 (MQ=255)
                                                                                   ttATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAgctgct                                                                 <  2:533809/150‑1 (MQ=255)
                                                                                   ttATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATAAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGcgggcagcagctgattggccgacgttttgtgctgga                                                                 >  1:342375‑M1/1‑114 (MQ=255)
                                                                                    tATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGctgctg                                                                <  2:1428757/150‑1 (MQ=255)
                                                                                        ccTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCcg                                                            >  1:209737/1‑150 (MQ=255)
                                                                                           tCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCAATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGcgc                                                         >  2:536019‑M2/1‑147 (MQ=255)
                                                                                              aTCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGcgctgg                                                      <  1:731389‑M2/150‑7 (MQ=255)
                                                                                              aTCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAAGCAGCTGCTGCCCGcgctgg                                                      >  1:98444‑M2/1‑144 (MQ=255)
                                                                                                cGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATAAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGcgggcagcagctgattggccgacgttttgtgctggaatctccgctggtc                                                    <  2:342375‑M1/150‑50 (MQ=255)
                                                                                                    aTGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGcgggcagcagctgattggccgacgttttgtgctggaatctccgctggtcaacg                                                <  2:1006800‑M1/150‑54 (MQ=255)
                                                                                                    aTGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGcgggcagcagctgattggccgacgttttgtgctggaatctccgctggtcaacg                                                <  1:630597‑M1/150‑54 (MQ=255)
                                                                                                     tGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAAt                                               <  1:806124/150‑1 (MQ=255)
                                                                                                     tGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAAt                                               <  2:731389/150‑1 (MQ=255)
                                                                                                        cTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGAc                                            >  2:1183384/1‑150 (MQ=255)
                                                                                                         ttCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACt                                           <  1:1372204/150‑1 (MQ=255)
                                                                                                           cAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGACCTGTCACCGACGCGCATATTAACGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGACAATCAGCTGCTGCCCGCGCTGGTTTAAATGTCTAt                                         >  2:696294/1‑150 (MQ=255)
                                                                                                            aaCATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATc                                        >  1:31554/1‑150 (MQ=255)
                                                                                                              cATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGc                                      <  2:1433514/150‑1 (MQ=255)
                                                                                                              cATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGc                                      <  1:1221703/150‑1 (MQ=255)
                                                                                                                  cGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCtgtg                                  <  2:1336964/150‑1 (MQ=255)
                                                                                                                  cGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCtgtg                                  <  1:224211/150‑1 (MQ=255)
                                                                                                                   gTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCtgtgg                                 >  1:435387/1‑149 (MQ=255)
                                                                                                                     aCCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCtt                               >  2:1158595/1‑150 (MQ=255)
                                                                                                                      ccTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCTGCGCTGGTTTAAATGACTATCGCTGTGCttt                              <  2:730436/150‑1 (MQ=255)
                                                                                                                      ccTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATTACTATCGCTGTGCttt                              >  2:1144779/1‑150 (MQ=255)
                                                                                                                      ccTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCttt                              <  1:1042852/150‑1 (MQ=255)
                                                                                                                              gagaTCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTcgcg                      <  2:384783/150‑1 (MQ=255)
                                                                                                                              gagaTCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTcgcg                      >  2:1673750/1‑150 (MQ=255)
                                                                                                                                  tCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCAcc                  <  2:493382/150‑1 (MQ=255)
                                                                                                                                  tCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTGTGTTCGCGcccc                  >  2:887931/1‑150 (MQ=255)
                                                                                                                                       gCTGGCGCTGACAGACCATGAACTGTCACCGACGCGCATATGACCGCGCCCGGTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACccgccg             <  2:1094418/150‑1 (MQ=255)
                                                                                                                                          gtcggtGACAGTTCATGGCCTGTCAGCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGa          >  1:1394953/6‑150 (MQ=255)
                                                                                                                                            cgcTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACa        >  2:431770/1‑150 (MQ=255)
                                                                                                                                             ggtGACAGTTCATGGCCTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACaa       >  1:802072/3‑150 (MQ=255)
                                                                                                                                              cTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAAc      >  2:1490931/1‑150 (MQ=255)
                                                                                                                                               tGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAAcc     <  2:582796/150‑1 (MQ=255)
                                                                                                                                                  cAGGCCATGAACTGTCACCGCCGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCAtt  >  2:371177/1‑150 (MQ=255)
                                                                                                                                                  cAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCAtt  >  2:189619/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GACTGCCCGATTATCGAAAATCTCTCCGGCTGCTGTCATCATCGGGCAGGGTGATTAAGACGAGACGGCTCGGATATGTAGGGTTATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATT  >  NC_000913/514347‑514642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: