Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,189,607 C→A 5.3% intergenic (+34/‑179) rpoC → / → yjaZ RNA polymerase, beta prime subunit/stationary phase growth adaptation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,189,6070CA 5.3% 454.7 / 7.4 150intergenic (+34/‑179)rpoC/yjaZRNA polymerase, beta prime subunit/stationary phase growth adaptation protein
Reads supporting (aligned to +/- strand):  ref base C (78/63);  new base A (4/5);  total (82/68)
Fisher's exact test for biased strand distribution p-value = 7.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 152 total aligned reads displayed.

TGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACT  >  NC_000913/4189458‑4189755
                                                                                                                                                     |                                                                                                                                                     
tGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTc                                                                                                                                                       >  1:812653/1‑150 (MQ=255)
   gTCGCCGTGCTGCGGGTGATGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGGCTGGGCGGATCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTcggc                                                                                                                                                    <  2:1584680/150‑1 (MQ=255)
        cGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGtt                                                                                                                                              >  2:1227409/1‑150 (MQ=255)
        cGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGtt                                                                                                                                              <  1:818334/150‑1 (MQ=255)
           gctgcGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGttttt                                                                                                                                           >  1:552095/1‑150 (MQ=255)
            ctgcGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGtttttt                                                                                                                                          <  1:1391569/150‑1 (MQ=255)
                gggTGAAGCTCCGGCTGCACCGCAGGTGTCTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATg                                                                                                                                      >  2:1698816/1‑150 (MQ=255)
                gggTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATg                                                                                                                                      >  2:17686/1‑150 (MQ=255)
                 ggTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATgg                                                                                                                                     <  2:82485/150‑1 (MQ=255)
                     aaGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATgggggg                                                                                                                                 >  1:403526/1‑150 (MQ=255)
                        cgccGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGGTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATTGGGGGAGt                                                                                                                              <  2:1066164/148‑1 (MQ=255)
                             gCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAgg                                                                                                                         <  1:228851/150‑1 (MQ=255)
                             gCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCACCCTCGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACTTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAgg                                                                                                                         <  1:821888/150‑1 (MQ=255)
                              cTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAggg                                                                                                                        >  2:1720157/1‑150 (MQ=255)
                                 cACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGaaa                                                                                                                     >  2:1283483/1‑150 (MQ=255)
                                   ccGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGCACGCACGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCTCTTCGGCGGGTTTTTTTATGGGGTGTGTTTAGGGAAagc                                                                                                                   >  1:81287/1‑149 (MQ=255)
                                   ccGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAaga                                                                                                                   >  1:784950/1‑150 (MQ=255)
                                   ccGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAaga                                                                                                                   >  2:780832/1‑150 (MQ=255)
                                   ccGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAaga                                                                                                                   <  1:1472019/150‑1 (MQ=255)
                                   ccGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAaga                                                                                                                   >  2:774316/1‑150 (MQ=255)
                                    cGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAagag                                                                                                                  <  2:665900/150‑1 (MQ=255)
                                    cGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAagag                                                                                                                  <  2:1026898/150‑1 (MQ=255)
                                       aGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCAt                                                                                                               <  1:172481/150‑1 (MQ=255)
                                       aGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCAt                                                                                                               >  1:1447380/1‑150 (MQ=255)
                                        ggTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCAtt                                                                                                              >  1:1637195/1‑150 (MQ=255)
                                        ggTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCAtt                                                                                                              <  2:528758/150‑1 (MQ=255)
                                            aCTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTc                                                                                                          >  1:695939/1‑150 (MQ=255)
                                             cTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCa                                                                                                         >  2:1178366/1‑150 (MQ=255)
                                              tGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAg                                                                                                        >  1:1483790/1‑150 (MQ=255)
                                              tGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAg                                                                                                        <  1:341728/150‑1 (MQ=255)
                                              ggCAGACGCCGCATCTGCCAGCCAGGCAGAACTGCTGAACGCAGGGCTGGGCGGTCCTGATAACGCGTAATCGTTAATCCGCAAATAACGTAAAACCCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAg                                                                                                        <  2:235319/149‑1 (MQ=255)
                                                     agcagttCTGCCAGGCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATAttt                                                                                                 >  1:1039334/7‑150 (MQ=255)
                                                       cGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGTGTTTAGGGAAAGGCCATTTGTCAGAATATTTaa                                                                                               >  2:854460/1‑150 (MQ=255)
                                                         gttCTGCCAGGCTGGCAGAACTGCTGAACGCAGGTCTGGGGGGTTCTGATAACGAGGAATCGTTAATCGGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGATTATTTAAgg                                                                                             >  1:1485983/3‑150 (MQ=255)
                                                         cATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAgg                                                                                             >  2:670813/1‑150 (MQ=255)
                                                            cTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAAt                                                                                          >  1:573555/1‑150 (MQ=255)
                                                              gCCAGCCTGGCAGAACTGCTGAACGCAGGTCTTGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGACAGAGCATTTGTCAGAATATTTAAGGAAttt                                                                                        <  1:688537/150‑1 (MQ=255)
                                                                cAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTTATCGTTTATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCt                                                                                      <  1:603685/150‑1 (MQ=255)
                                                                   ccTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGaa                                                                                   >  2:402421/1‑150 (MQ=255)
                                                                     tGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATa                                                                                 >  2:1020365/1‑150 (MQ=255)
                                                                     tGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATa                                                                                 >  2:561104/1‑150 (MQ=255)
                                                                       gCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACt                                                                               >  1:881044/1‑150 (MQ=255)
                                                                       gCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACt                                                                               <  1:1227409/150‑1 (MQ=255)
                                                                        cAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTc                                                                              >  2:901441/1‑150 (MQ=255)
                                                                         aGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCa                                                                             <  2:1447380/150‑1 (MQ=255)
                                                                          gAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCAt                                                                            >  1:1612714/1‑150 (MQ=255)
                                                                           aaCTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATa                                                                           <  1:774316/150‑1 (MQ=255)
                                                                               gctgAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGATTTTCTGAATACTCATAATCa                                                                       >  1:380657/1‑150 (MQ=255)
                                                                                ctgAACGCAGGTCTGGGCGGTTCTGATATCGAGTAATCGTTATTCCGCAAATAAAGTAAAAAACCGCTTAGGCGGGTTTTTTTATGTGTGTAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCaa                                                                      <  1:277239/150‑1 (MQ=255)
                                                                                ctgAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTGTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCaa                                                                      >  1:307293/1‑150 (MQ=255)
                                                                                  gAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATg                                                                    <  2:1462080/150‑1 (MQ=255)
                                                                                  gAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATg                                                                    <  1:321767/150‑1 (MQ=255)
                                                                                     cGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTACGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTag                                                                 >  2:19932/1‑150 (MQ=255)
                                                                                     agCAGGTGTGGGCGGTTCTGATAACGACTTATCGTTAATTCTCAAATAACGTAAAATCCCTGTTCGTCGGTTTTTTTTATGTGTGGACTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATTATCAATGTag                                                                 <  1:893584/149‑1 (MQ=255)
                                                                                      gCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTaga                                                                <  1:314013/150‑1 (MQ=255)
                                                                                         ggTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGAt                                                             >  1:1575844/1‑150 (MQ=255)
                                                                                          gTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATg                                                            <  2:1524418/150‑1 (MQ=255)
                                                                                               ggCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTaa                                                       >  1:129330/1‑150 (MQ=255)
                                                                                                 cGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAata                                                     <  1:1178366/150‑1 (MQ=255)
                                                                                                  ggTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAatat                                                    >  2:411949/1‑150 (MQ=255)
                                                                                                  ggTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAatat                                                    >  1:115284/1‑150 (MQ=255)
                                                                                                   gTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATc                                                   >  2:309790/1‑150 (MQ=255)
                                                                                                     tCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCt                                                 <  2:80692/150‑1 (MQ=255)
                                                                                                      cTGATAACGATTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTg                                                <  1:949156/150‑1 (MQ=255)
                                                                                                      cTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGAAGAGATGACTAATATCATg                                                >  1:1451173/1‑150 (MQ=255)
                                                                                                        gATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGCTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGa                                               >  2:338686/1‑150 (MQ=255)
                                                                                                        gATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGTGGAGTTTAGGTAAAGAGGATTAGTCAGAATATTTAACGAATTCCTGAATACTCATAATGAATGTAGAGAGGACTAATATCCTGaa                                              >  2:1624146/1‑150 (MQ=255)
                                                                                                        gATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGaa                                              >  2:1081863/1‑150 (MQ=255)
                                                                                                          tAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAac                                            >  1:597253/1‑150 (MQ=255)
                                                                                                             cGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAactga                                         >  2:1635700/1‑150 (MQ=255)
                                                                                                               aGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAactgact                                       >  2:1484697/1‑150 (MQ=255)
                                                                                                               aGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAactgact                                       >  2:1679843/1‑150 (MQ=255)
                                                                                                                gTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAactgactg                                      >  1:1252685/1‑150 (MQ=255)
                                                                                                                  aaTCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATATTCAATGTAGAGATGACTAATATCCTGAAACTGACTGaa                                    >  1:623188/1‑150 (MQ=255)
                                                                                                                  aaTCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGaa                                    >  2:1480366/1‑150 (MQ=255)
                                                                                                                     cGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTa                                 >  1:1342165/1‑150 (MQ=255)
                                                                                                                     cGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTa                                 >  1:748671/1‑150 (MQ=255)
                                                                                                                     cGTTAATCCGCAAATAACGTAAAAACACGCTTCGGAGGGTTTTTTTATGGGGGGTGTTTAGGGATAGAGCATTTGTCAGAATATTTAAGGAATTTATGAATAATAATAATAAATTTAGAGATGACTAATATCATGAAACTGACTGAACTa                                 >  2:1056000/1‑150 (MQ=255)
                                                                                                                        tAATCCGCAAATAAAGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAAtt                              <  1:1401394/150‑1 (MQ=255)
                                                                                                                            ccGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGTGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGt                          <  1:195228/150‑1 (MQ=255)
                                                                                                                            ccGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGt                          <  2:253586/150‑1 (MQ=255)
                                                                                                                            ccGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGt                          <  2:872343/150‑1 (MQ=255)
                                                                                                                             cGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTc                         <  2:1536595/150‑1 (MQ=255)
                                                                                                                               cAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCaa                       >  2:1590157/1‑150 (MQ=255)
                                                                                                                                 aaTAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCAAAc                     <  1:901441/150‑1 (MQ=255)
                                                                                                                                     ataaaaaaaCCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCAAACTCgg                 >  1:718411/5‑150 (MQ=255)
                                                                                                                                     ataaaaaaaCCCGCCGAAGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCAAACTCgg                 >  1:1478276/5‑150 (MQ=255)
                                                                                                                                     aCGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAAGGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCAAACTCgg                 >  1:944204/1‑150 (MQ=255)
                                                                                                                                       gTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGATCATTTGTCAGAATATTTATGGAATTTCTGAATACTCATAATCATTGTAGAGATGACTAATATACTGAAACTGACTGAACAAATTTAGTCAAACTCGGCa               >  1:1583946/1‑150 (MQ=255)
                                                                                                                                       aaaaaaaCCCGCCGAAGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCATGAAACTGACTGAACTAATTGAGTCAAACTCGGCa               >  2:833604/3‑150 (MQ=255)
                                                                                                                                        tAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCAAACTCGGCaa              <  1:394270/150‑1 (MQ=255)
                                                                                                                                          aaaaCCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCAAACTCGGCAAgg            >  2:1357261/1‑150 (MQ=255)
                                                                                                                                           aaaCCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGa           >  1:173210/1‑150 (MQ=255)
                                                                                                                                           aaaCCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGa           <  1:783580/150‑1 (MQ=255)
                                                                                                                                            aaCCCGCTTCGGCGGGGTTTTTTATGGGGGGAGATTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGATTACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTCAACTAATTGAGTCAAACTCGTCAAGTAt          >  2:647923/1‑150 (MQ=255)
                                                                                                                                              cccGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTc        <  1:234335/150‑1 (MQ=255)
                                                                                                                                               ccGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCg       >  2:652784/1‑150 (MQ=255)
                                                                                                                                               ccGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCg       >  1:1035542/1‑150 (MQ=255)
                                                                                                                                                 gCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGAt     <  2:812653/150‑1 (MQ=255)
                                                                                                                                                    tCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACt  >  2:1147326/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGCGTCGCCGTGCTGCGGGTGAAGCTCCGGCTGCACCGCAGGTGACTGCAGAAGACGCATCTGCCAGCCTGGCAGAACTGCTGAACGCAGGTCTGGGCGGTTCTGATAACGAGTAATCGTTAATCCGCAAATAACGTAAAAACCCGCTTCGGCGGGTTTTTTTATGGGGGGAGTTTAGGGAAAGAGCATTTGTCAGAATATTTAAGGAATTTCTGAATACTCATAATCAATGTAGAGATGACTAATATCCTGAAACTGACTGAACTAATTGAGTCAAACTCGGCAAGGATTCGATACT  >  NC_000913/4189458‑4189755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: