Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,193,286 G→C 6.9% D97E (GAC→GAG) ‡ thiF ← adenylyltransferase, modifies ThiS C‑terminus

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,193,2860GC 6.9% 314.3 / 12.3 102D97E (GAC→GAG) ‡thiFadenylyltransferase, modifies ThiS C‑terminus
Reads supporting (aligned to +/- strand):  ref base G (48/47);  new base C (5/2);  total (53/49)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-01

Only 100 of 103 total aligned reads displayed.

ATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCGACGCCAGCGCCC  >  NC_000913/4193137‑4193433
                                                                                                                                                     |                                                                                                                                                   
aTAAGCGGCGTGTTGAGTGCCTCGCAGGAGGCATTAATCTACTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACGACATCGGCCGGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTTTAATGCTGTCAGTTGAATg                                                                                                                                                     <  2:1583006/150‑1 (MQ=255)
  aaGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTc                                                                                                                                                   >  2:1350845/1‑150 (MQ=255)
  aaGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTc                                                                                                                                                   >  2:1545067/1‑150 (MQ=255)
   aGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCg                                                                                                                                                  >  2:626959/1‑150 (MQ=255)
    gcggcgTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTATTGCTGTCAGTTGAATGTCgg                                                                                                                                                 <  1:238789/150‑1 (MQ=255)
    cctgcgTGTTGAGAGCCACGCAGGCGGCATTAATCTCCTGCCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCgg                                                                                                                                                 <  2:327222/147‑1 (MQ=255)
        cgTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAATGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGAtt                                                                                                                                             >  2:1247637/1‑150 (MQ=255)
        cgTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGagt                                                                                                                                             >  1:747038/1‑148 (MQ=255)
        cgTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGAtt                                                                                                                                             >  2:514180/1‑150 (MQ=255)
         gtgtTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTc                                                                                                                                            <  2:1034438/150‑1 (MQ=255)
          tgtTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCa                                                                                                                                           >  2:939005/1‑150 (MQ=255)
            ttGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAAc                                                                                                                                         <  1:247315/150‑1 (MQ=255)
              gAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATCCCGACATTCAACtg                                                                                                                                       <  1:1354292/150‑1 (MQ=255)
               aGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCATCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACtgt                                                                                                                                      <  1:1664649/150‑1 (MQ=255)
                gTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACtgtg                                                                                                                                     >  2:1162873/1‑150 (MQ=255)
                gTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACtgtg                                                                                                                                     >  2:1268414/1‑150 (MQ=255)
                 tGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCTCATCGGCCCGGGCAACCGCAGCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTgtgt                                                                                                                                    <  1:802624/150‑1 (MQ=255)
                  gCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGCGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTGGTTGTAATGCTGTCAGTTGAATGTCGGGATTCACCTGTGTc                                                                                                                                   >  1:118319/1‑150 (MQ=255)
                  gCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTc                                                                                                                                   >  2:503311/1‑150 (MQ=255)
                  gCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTAGGGATTAAACTttgta                                                                                                                                   >  1:365506/1‑149 (MQ=255)
                  gCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATCCCGACATTCAACtgacag                                                                                                                                   <  1:1244713/150‑5 (MQ=255)
                        cAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAGCCGTTGTTGTAATGCTGTCAGTTGAATGTCGGTATTCAACTGTGTCAGTCGc                                                                                                                             >  2:54126/1‑150 (MQ=255)
                           gcggcATTAATCTCCTGGCGAGTCGCCATATTCTCGGTACAGTCGAGCACCACAACGGCCCGTGCAACCGCATCTTTTAAAGCCTCACACGTTAACCGTTGTTGTAATGCAGTCAGTAGAATGTCGGGATTCAACTGTGTCAGTGGCTGt                                                                                                                          >  2:284586/1‑150 (MQ=255)
                              gcATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTgg                                                                                                                       >  1:1454739/1‑150 (MQ=255)
                                 ttAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCAGCTTTTAACGCCTCCCCCCTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTg                                                                                                                    <  2:1081797/150‑1 (MQ=255)
                                   aaTCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGATTGTCGGGATTCAACTGTGTc                                                                                                                                   >  1:825957/1‑133 (MQ=255)
                                   aaTCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTc                                                                                                                                   <  2:825957/133‑1 (MQ=255)
                                   aaTCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGAc                                                                                                                  <  2:698774/150‑1 (MQ=255)
                                    aTCTCCTGGCGAGTCTCCATATTGTCGGTACAGTCTAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCAGCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGAct                                                                                                                 <  1:284586/150‑2 (MQ=255)
                                    aTCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGAcc                                                                                                                 >  1:1320321/1‑150 (MQ=255)
                                    aTCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGAcc                                                                                                                 <  2:34805/150‑1 (MQ=255)
                                    aTCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGAcc                                                                                                                 <  2:370969/150‑1 (MQ=255)
                                    aTCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGAcc                                                                                                                 <  1:626959/150‑1 (MQ=255)
                                      ctcCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTg                                                                                                               >  2:880457/1‑150 (MQ=255)
                                        ccTTGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGGCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCg                                                                                                             <  1:1082358/150‑1 (MQ=255)
                                        ccTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGGTGTAATGCTGTCAGTGGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCg                                                                                                             >  1:1424175/1‑150 (MQ=255)
                                                 gcGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGc                                                                                                    <  2:224626/149‑1 (MQ=255)
                                                     cATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGcgatc                                                                                                >  1:537549/1‑150 (MQ=255)
                                                     cATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGcgatc                                                                                                <  1:122795/150‑1 (MQ=255)
                                                          tGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGatat                                                                                           >  1:826984/1‑150 (MQ=255)
                                                           gTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACACGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATc                                                                                          >  1:784473/1‑150 (MQ=255)
                                                            tCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCt                                                                                         <  2:1336411/150‑1 (MQ=255)
                                                              ggTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGGCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTc                                                                                       <  1:1101581/150‑1 (MQ=255)
                                                              ggTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCATCACCCGGTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTTTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTc                                                                                       >  1:856220/1‑150 (MQ=255)
                                                               gTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCa                                                                                      <  2:491484/150‑1 (MQ=255)
                                                                    gTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGt                                                                                 >  2:714143/1‑150 (MQ=255)
                                                                    gTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGt                                                                                 >  1:427461/1‑150 (MQ=255)
                                                                          caccacATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAagag                                                                           >  2:339067/1‑150 (MQ=255)
                                                                           accacATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTATTGCTGTCAGTTGAATGTCGGGCTTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAgg                                                                          >  1:1581004/1‑150 (MQ=255)
                                                                            ccacATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGa                                                                         >  2:799728/1‑150 (MQ=255)
                                                                              acaTCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGTCGATCGATATCTTCAGTGGTAAAGAGGAtt                                                                       <  2:736714/150‑1 (MQ=255)
                                                                                aTCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTg                                                                     <  2:1320321/150‑1 (MQ=255)
                                                                                aTCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTg                                                                     <  1:1725835/150‑1 (MQ=255)
                                                                                  cGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTc                                                                   <  2:1538441/150‑1 (MQ=255)
                                                                                   ggCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCgg                                                                                                      <  2:912844/114‑1 (MQ=255)
                                                                                   ggCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCgg                                                                                                      >  1:912844/1‑114 (MQ=255)
                                                                                   ggCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCg                                                                  <  2:397255/150‑1 (MQ=255)
                                                                                    gCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGACTGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGt                                                                 <  2:926133/150‑1 (MQ=255)
                                                                                     cccGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATTTCTTCAGTGGTAAAGAGGATTTGTCGtt                                                                >  1:1549801/1‑150 (MQ=255)
                                                                                       cGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGc                                                              >  2:255737/1‑150 (MQ=255)
                                                                                        gTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCa                                                             <  2:1139597/150‑1 (MQ=255)
                                                                                          gCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGa                                                           <  1:1158369/150‑1 (MQ=255)
                                                                                                gCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCtt                                                     <  1:1214086/150‑1 (MQ=255)
                                                                                                 cATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTa                                                    <  1:1438237/150‑1 (MQ=255)
                                                                                                 cATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGATGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTa                                                    >  1:1553391/1‑150 (MQ=255)
                                                                                                     ttttAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATg                                                <  1:1440258/150‑1 (MQ=255)
                                                                                                     ttttAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATg                                                >  1:150914/1‑150 (MQ=255)
                                                                                                        tAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGcac                                             <  2:1442055/150‑1 (MQ=255)
                                                                                                         aaCGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGcaca                                            >  2:716259/1‑150 (MQ=255)
                                                                                                                cACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATcgtcgt                                     <  1:541406/150‑1 (MQ=255)
                                                                                                                    cGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATc                                 >  2:1494125/1‑150 (MQ=255)
                                                                                                                      ttAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTg                               <  1:1162873/150‑1 (MQ=255)
                                                                                                                      ttAACCGCTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTg                               <  2:645739/150‑1 (MQ=255)
                                                                                                                        aaCCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGcc                             <  2:1100636/150‑1 (MQ=255)
                                                                                                                          ccGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAg                           >  1:1172335/1‑150 (MQ=255)
                                                                                                                          ccGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAg                           <  1:832547/150‑1 (MQ=255)
                                                                                                                            gttgttGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTa                         >  2:1660833/1‑150 (MQ=255)
                                                                                                                               gttgtAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCa                      >  2:413263/1‑150 (MQ=255)
                                                                                                                                ttgtACTGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAg                     <  1:1247637/150‑1 (MQ=255)
                                                                                                                                  gtAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCg                   >  2:523389/1‑150 (MQ=255)
                                                                                                                                  gtAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCg                   >  1:1085479/1‑150 (MQ=255)
                                                                                                                                  gtAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCg                   <  1:1350845/150‑1 (MQ=255)
                                                                                                                                  gtAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCg                   <  1:1545067/150‑1 (MQ=255)
                                                                                                                                    aaTGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTc                 <  2:1561399/150‑1 (MQ=255)
                                                                                                                                    aaTGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTc                 >  2:891873/1‑150 (MQ=255)
                                                                                                                                       gCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCg              <  2:1454739/150‑1 (MQ=255)
                                                                                                                                       gCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCg              <  1:880457/150‑1 (MQ=255)
                                                                                                                                         acacAGTTGAATCCCGACATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCGAc            >  2:782335/4‑150 (MQ=255)
                                                                                                                                          cacAGTTGAATCCCGACATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCGACg           >  1:1305784/3‑150 (MQ=255)
                                                                                                                                          cacAGTTGAATCCCGACATCCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCGACg           >  1:424972/3‑150 (MQ=255)
                                                                                                                                            cAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCGACGcc         <  2:1227328/150‑1 (MQ=255)
                                                                                                                                            cAGTTGAATCCCGACATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCGACGcc         >  2:1661428/1‑150 (MQ=255)
                                                                                                                                             aGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCGACGccc        >  2:1463179/1‑149 (MQ=255)
                                                                                                                                             aGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCGACGCCa        >  2:813581/1‑150 (MQ=255)
                                                                                                                                             aGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGAACATCGTCGTCATCTGCAAGTACCAGCGTCCCGACGCCa        >  2:231164/1‑150 (MQ=255)
                                                                                                                                              gTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCTGACGCCAg       >  2:1223663/1‑150 (MQ=255)
                                                                                                                                              gTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCGACGCCAg       >  1:815303/1‑150 (MQ=255)
                                                                                                                                                  aaTGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCGACGCCAGcgcc   <  2:537549/150‑1 (MQ=255)
                                                                                                                                                  aaTGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCGACGCCAGcgcc   >  2:448187/1‑150 (MQ=255)
                                                                                                                                                   aTGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCGACGCCAGcgccc  >  2:188733/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ATAAGCGGCGTGTTGAGTGCCACGCAGGCGGCATTAATCTCCTGGCGAGTCGCCATATTGTCGGTACAGTCGAGCACCACATCGGCCCGTGCAACCGCATCTTTTAACGCCTCACCCGTTAACCGTTGTTGTAATGCTGTCAGTTGAATGTCGGGATTCAACTGTGTCAGTCGCTGTTGGCTGACCTGCGATTTCGGGCGATCGATATCTTCAGTGGTAAAGAGGATTTGTCGTTGCAGATTGCTTAAATGCACATCGTCGTCATCTGCCAGTACCAGCGTCCCGACGCCAGCGCCC  >  NC_000913/4193137‑4193433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: