Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,256,363 A→T 5.5% L35Q (CTG→CAG)  plsB ← glycerol‑3‑phosphate O‑acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,256,3630AT 5.5% 414.0 / 8.5 140L35Q (CTG→CAG) plsBglycerol‑3‑phosphate O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (67/64);  new base T (5/3);  total (73/67)
Fisher's exact test for biased strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

TGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGA  >  NC_000913/4256214‑4256512
                                                                                                                                                     |                                                                                                                                                     
tgaaCACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCTTGTGCCAGACACTGGGCGCGCAACGTCCGCAAATCTGCTTTCGAGTTGTACGGTAACACGTACATAATTGGACGAGAGGTATCCa                                                                                                                                                       >  2:47944/1‑150 (MQ=255)
tgaaCACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCa                                                                                                                                                       >  2:857283/1‑150 (MQ=255)
tgaaCACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCa                                                                                                                                                       >  2:419542/1‑150 (MQ=255)
  aaCACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTAAATAATTGGACGAGAGGTATCCAGc                                                                                                                                                     >  2:63618/1‑150 (MQ=255)
    cacaTAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGccc                                                                                                                                                   >  2:1065224/1‑150 (MQ=255)
     acaTAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGcccc                                                                                                                                                  >  2:1700007/1‑150 (MQ=255)
      caTAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTACCGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCa                                                                                                                                                 >  1:653007/1‑150 (MQ=255)
      caTAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGCACGAGAGGTATCCAGCCCCa                                                                                                                                                 >  2:130481/1‑150 (MQ=255)
         aGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGtt                                                                                                                                              >  1:1446608/1‑150 (MQ=255)
          gcgcGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGATc                                                                                                                                             >  2:421067/1‑150 (MQ=255)
           cgcgGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTcc                                                                                                                                            >  2:1181155/1‑150 (MQ=255)
             cgGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCgg                                                                                                                                          <  2:420584/150‑1 (MQ=255)
                cAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCACCGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGc                                                                                                                                       >  1:320410/1‑150 (MQ=255)
                cAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGc                                                                                                                                       >  1:769697/1‑150 (MQ=255)
                 aGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCa                                                                                                                                      <  1:158404/150‑1 (MQ=255)
                 aGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCa                                                                                                                                      <  2:569054/150‑1 (MQ=255)
                 aGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCa                                                                                                                                      <  1:1275030/150‑1 (MQ=255)
                  gTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCCTGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAg                                                                                                                                     <  1:1190920/150‑1 (MQ=255)
                    aGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGa                                                                                                                                   <  1:479262/150‑1 (MQ=255)
                     gCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGAt                                                                                                                                  >  1:578531/1‑150 (MQ=255)
                       gTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGCGTTGTACGTTAAAACGTACATAATTTGACGAGAGGTATCCAGCCCCAGTTCAGGGGAAGTATCt                                                                                                                                >  1:1275651/1‑150 (MQ=255)
                       gTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCTGCCCCGGAACTGGGGCTGGAtac                                                                                                                                <  2:934084/150‑3 (MQ=255)
                          ccGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCTGCCCCGGAACTGGGGCTGGAtacctc                                                                                                                             <  1:724179/150‑6 (MQ=255)
                           cGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCg                                                                                                                            <  2:581622/150‑1 (MQ=255)
                            gTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCgg                                                                                                                           >  1:867925/1‑150 (MQ=255)
                               gATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAAt                                                                                                                        <  1:1268158/150‑1 (MQ=255)
                                aTTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCCGCAAATCTGCTTTCGAGTTGTACGGGAAAACGTACATAATTGGACGAGAGGTATCCCGCCCCAGTTCCGGGGCAGGAGCTGCCGGAAtt                                                                                                                       >  1:1435448/1‑149 (MQ=255)
                                         ggCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCtt                                                                                                              <  2:800927/150‑1 (MQ=255)
                                          gCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCttt                                                                                                             >  1:1675302/1‑150 (MQ=255)
                                             ctAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCtttttt                                                                                                          >  1:1100420/1‑149 (MQ=255)
                                             ctAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTa                                                                                                          <  1:1656701/150‑1 (MQ=255)
                                             ctAACGGGTCAGGCAAGTCATGAGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTa                                                                                                          <  1:1057391/150‑1 (MQ=255)
                                             ctAACGGGACAGGCAAGTCATGTGCCAGACACTGGGCCCGCAACGTCAGCAAAACTGCTTTCGAGTTGTACGGGTTCACGTGCATTATTGGATGAGAGGTTTCCTGCCCCAGTTCCGGGGCCGGATCTGCGGGAATAGACTTGcagtgta                                                                                                          >  2:328613/1‑144 (MQ=255)
                                             ctAACGCGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGTAATAGACTTGCTTTTTa                                                                                                          <  1:895392/150‑1 (MQ=255)
                                              tAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTAc                                                                                                         <  2:1311891/150‑1 (MQ=255)
                                              tAACGGGGCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTAc                                                                                                         <  1:1139844/150‑1 (MQ=255)
                                              aaaCGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCGTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTAc                                                                                                         <  1:838573/149‑1 (MQ=255)
                                              aaaCGGGTCAGGCAAGGCCTGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTAc                                                                                                         <  1:1078712/149‑1 (MQ=255)
                                                aCGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCa                                                                                                       >  1:61567/1‑150 (MQ=255)
                                                        ggCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAACCGTTCCTAATTGGGCGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGAACTGGCGGATTAGCCTTGGTTTTTTCCTGGATGGtt                                                                                               >  2:1146551/1‑150 (MQ=255)
                                                         gCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTa                                                                                              >  1:983559/1‑150 (MQ=255)
                                                         gCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTa                                                                                              >  1:870584/1‑150 (MQ=255)
                                                          cAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTaa                                                                                             >  2:91375/1‑150 (MQ=255)
                                                           aaGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAAt                                                                                            >  2:833299/1‑150 (MQ=255)
                                                             gTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGGGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATgg                                                                                          >  1:784393/1‑150 (MQ=255)
                                                             gTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATgg                                                                                          >  1:546835/1‑150 (MQ=255)
                                                              tCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGt                                                                                         <  2:1446608/150‑1 (MQ=255)
                                                               cATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTa                                                                                        <  1:857556/150‑1 (MQ=255)
                                                                 tgtgCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTaaa                                                                                      <  2:772845/150‑1 (MQ=255)
                                                                   tgCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGCCTTGCTTTTTACCAGGATGCTTAAGGGTAAAtt                                                                                    >  2:303275/1‑150 (MQ=255)
                                                                    gCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAACCGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGCTGCTGAATGGTAAATTc                                                                                   >  2:175317/1‑150 (MQ=255)
                                                                    gCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTc                                                                                   >  1:327803/1‑150 (MQ=255)
                                                                      cAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGATGTATCCAGCCCCTGTTCCGGGGCCGGATCTGCCGGAATAGAATTGCTTTTTACCAGGATGCTTAATTGTAAATTCAg                                                                                 >  2:293189/1‑150 (MQ=255)
                                                                       aGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTTGACGAGAGGTATCCATCCCCAGTTCCGGGGCAGGATCTGCCGTAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGt                                                                                <  1:857283/150‑1 (MQ=255)
                                                                        gACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATgg                                                                                          >  2:205454/1‑139 (MQ=255)
                                                                        gACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATgg                                                                                          <  1:205454/139‑1 (MQ=255)
                                                                          cacTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAAt                                                                             >  1:1463983/1‑150 (MQ=255)
                                                                          cacTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAAt                                                                             <  2:962224/150‑1 (MQ=255)
                                                                           acTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAAtt                                                                            >  2:1474025/1‑150 (MQ=255)
                                                                            cTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAAttt                                                                           <  2:600960/150‑1 (MQ=255)
                                                                             tGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTTCATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTGAATGGTAAATTCAGTAAtttt                                                                          >  1:338401/1‑149 (MQ=255)
                                                                                gcgcgcAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTgtag                                                                       <  2:870584/150‑1 (MQ=255)
                                                                                   cgcAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTaa                                                                    >  2:1526989/1‑150 (MQ=255)
                                                                                     cAACGTCAGCAATTCTGCGTTCGCGTTGTACGGTAAACCGTACATAATTGGACGAGAGGGTTACATCCACTGATACGGGGCAGTATCTGACTCAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAAt                                                                  <  1:293189/150‑1 (MQ=255)
                                                                                     cAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAAt                                                                  <  1:1382723/150‑1 (MQ=255)
                                                                                           cAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTgg                                                            <  1:579113/150‑1 (MQ=255)
                                                                                           cAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTgg                                                            <  1:1001125/150‑1 (MQ=255)
                                                                                           cAGCAAATCAGCTTTCGAGTTTTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTgg                                                            <  2:868986/150‑1 (MQ=255)
                                                                                             gCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTAAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGcc                                                          <  2:1374434/150‑1 (MQ=255)
                                                                                               aaaTCTGCTTTCGAGTTGTTCGGTAAAAAGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGGAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAg                                                        <  1:914258/150‑1 (MQ=255)
                                                                                               aaaTCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAg                                                        <  1:1195177/150‑1 (MQ=255)
                                                                                                  tCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTGAATTCGTGGCCAGCCg                                                     <  1:1691691/150‑1 (MQ=255)
                                                                                                    tGCTTTCGAGTTGTACTGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGa                                                   <  1:446343/150‑1 (MQ=255)
                                                                                                      cTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGATTATACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACa                                                 <  1:1150026/150‑1 (MQ=255)
                                                                                                      cTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACa                                                 >  1:156965/1‑150 (MQ=255)
                                                                                                      cTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACa                                                 >  1:1475479/1‑150 (MQ=255)
                                                                                                      cTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACa                                                 <  2:769697/150‑1 (MQ=255)
                                                                                                                    ggTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAg                                   >  1:250792/1‑150 (MQ=255)
                                                                                                                      tAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGcc                                 <  1:1105485/150‑1 (MQ=255)
                                                                                                                            gTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGt                           >  2:525883/1‑150 (MQ=255)
                                                                                                                            gTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGt                           >  1:1347150/1‑150 (MQ=255)
                                                                                                                              aCATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTta                         <  2:1321224/150‑1 (MQ=255)
                                                                                                                                aTAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTtaat                       <  2:983559/150‑1 (MQ=255)
                                                                                                                                aTAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTtaat                       <  2:1462616/150‑1 (MQ=255)
                                                                                                                                   aTTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTaataat                    >  1:396122/1‑150 (MQ=255)
                                                                                                                                    ttGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATg                   <  1:1319295/150‑1 (MQ=255)
                                                                                                                                      ggACGAGAGGTATCCATCCCCAGTTCCGGGGCAGGAGCTGCCGGAATAGACTTGCTTGTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCa                 <  2:1323387/150‑1 (MQ=255)
                                                                                                                                      ggACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCa                 >  1:388767/1‑150 (MQ=255)
                                                                                                                                      ggACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCa                 <  2:1128765/150‑1 (MQ=255)
                                                                                                                                        aCGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCaaa               >  2:523332/1‑150 (MQ=255)
                                                                                                                                        aCGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCaaa               <  1:54400/150‑1 (MQ=255)
                                                                                                                                        aCGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCaaa               <  2:1675302/150‑1 (MQ=255)
                                                                                                                                         cGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGTACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAAt              >  2:19783/1‑150 (MQ=255)
                                                                                                                                           agagGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATgc            >  1:952302/1‑150 (MQ=255)
                                                                                                                                              ggTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGcgcg         <  1:1662614/150‑1 (MQ=255)
                                                                                                                                               gTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCgg        >  2:558852/1‑150 (MQ=255)
                                                                                                                                                gaTCCTGCCCCGGAACTGGGGCTGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGc       >  2:1369851/2‑150 (MQ=255)
                                                                                                                                                   ccAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAg    >  2:700890/1‑150 (MQ=255)
                                                                                                                                                     aGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGa  >  1:688352/1‑150 (MQ=255)
                                                                                                                                                     aGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCAGCTTAATAATGCAAATGCGCGGCAAGGa  >  1:153664/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGA  >  NC_000913/4256214‑4256512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑