Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,256,374 C→T 5.8% P31P (CCG→CCA)  plsB ← glycerol‑3‑phosphate O‑acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,256,3740CT 5.8% 434.2 / 9.4 137P31P (CCG→CCA) plsBglycerol‑3‑phosphate O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (63/65);  new base T (4/4);  total (68/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.68e-01

Only 100 of 140 total aligned reads displayed.

CGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGT  >  NC_000913/4256225‑4256523
                                                                                                                                                     |                                                                                                                                                     
cgcgGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTcc                                                                                                                                                       >  2:1181155/1‑150 (MQ=255)
  cgGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCgg                                                                                                                                                     <  2:420584/150‑1 (MQ=255)
     cAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCACCGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGc                                                                                                                                                  >  1:320410/1‑150 (MQ=255)
     cAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGc                                                                                                                                                  >  1:769697/1‑150 (MQ=255)
      aGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCa                                                                                                                                                 <  1:158404/150‑1 (MQ=255)
      aGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCa                                                                                                                                                 <  1:1275030/150‑1 (MQ=255)
       gTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCCTGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAg                                                                                                                                                <  1:1190920/150‑1 (MQ=255)
         aGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGa                                                                                                                                              >  2:579113/1‑150 (MQ=255)
          gCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCGGAACTGGGGCTGGAt                                                                                                                                             <  2:816917/150‑1 (MQ=255)
          gCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGAt                                                                                                                                             >  1:578531/1‑150 (MQ=255)
            gTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGCGTTGTACGTTAAAACGTACATAATTTGACGAGAGGTATCCAGCCCCAGTTCAGGGGAAGTATCt                                                                                                                                           >  1:1275651/1‑150 (MQ=255)
            gTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCTGCCCCGGAACTGGGGCTGGAtac                                                                                                                                           <  2:934084/150‑3 (MQ=255)
             tGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCTGCCCCGGAACTGGGGCTGGAtacc                                                                                                                                          <  2:902173/150‑4 (MQ=255)
               ccGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCTGCCCCGGAACTGGGGCTGGAtacctc                                                                                                                                        <  1:724179/150‑6 (MQ=255)
                cGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCg                                                                                                                                       <  2:581622/150‑1 (MQ=255)
                 gTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCgg                                                                                                                                      >  1:867925/1‑150 (MQ=255)
                    gATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAAt                                                                                                                                   <  1:1268158/150‑1 (MQ=255)
                    gATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAAt                                                                                                                                   >  2:710/1‑150 (MQ=255)
                     aTTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCCGCAAATCTGCTTTCGAGTTGTACGGGAAAACGTACATAATTGGACGAGAGGTATCCCGCCCCAGTTCCGGGGCAGGAGCTGCCGGAAtt                                                                                                                                  >  1:1435448/1‑149 (MQ=255)
                          cAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACtt                                                                                                                             >  2:419592/1‑150 (MQ=255)
                              ggCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCtt                                                                                                                         <  2:800927/150‑1 (MQ=255)
                               gCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCttt                                                                                                                        >  1:1675302/1‑150 (MQ=255)
                                  ctAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCtttttt                                                                                                                     >  1:1100420/1‑149 (MQ=255)
                                  ctAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTa                                                                                                                     <  1:1656701/150‑1 (MQ=255)
                                  ctAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTa                                                                                                                     >  1:368192/1‑150 (MQ=255)
                                  ctAACGGGTCAGGCAAGTCATGAGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTa                                                                                                                     <  1:1057391/150‑1 (MQ=255)
                                  ctAACGCGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGTAATAGACTTGCTTTTTa                                                                                                                     <  1:895392/150‑1 (MQ=255)
                                   tAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTAc                                                                                                                    <  2:1311891/150‑1 (MQ=255)
                                   tAACGGGGCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTAc                                                                                                                    <  1:1139844/150‑1 (MQ=255)
                                   aaaCGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCGTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTAc                                                                                                                    <  1:838573/149‑1 (MQ=255)
                                     aCGGGTCCGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGAAAAACGTACATAATTGGACGATAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCa                                                                                                                  <  1:480283/150‑1 (MQ=255)
                                     aCGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCa                                                                                                                  >  1:61567/1‑150 (MQ=255)
                                      cGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAg                                                                                                                 >  1:1083189/1‑150 (MQ=255)
                                             ggCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAACCGTTCCTAATTGGGCGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGAACTGGCGGATTAGCCTTGGTTTTTTCCTGGATGGtt                                                                                                          >  2:1146551/1‑150 (MQ=255)
                                             ggCAAGTCATGTGCCAGACACTGGGCGCGCAACGTAAGCTAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCGCAGTTCCGGGGCAGGTTCTGCCGGAATAGACTGGCTTTTTAGCAGGATGGtt                                                                                                          >  1:1132980/1‑150 (MQ=255)
                                              gCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTa                                                                                                         >  1:983559/1‑150 (MQ=255)
                                               cAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTaa                                                                                                        >  2:91375/1‑150 (MQ=255)
                                                 aGTCATGGGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATg                                                                                                      <  2:1553104/150‑1 (MQ=255)
                                                   tCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGt                                                                                                    <  2:1446608/150‑1 (MQ=255)
                                                    cATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTa                                                                                                   <  1:857556/150‑1 (MQ=255)
                                                     aTGTTCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACTAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTaa                                                                                                  <  1:1563032/150‑1 (MQ=255)
                                                      tgtgCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTaaa                                                                                                 <  2:772845/150‑1 (MQ=255)
                                                        tgCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGCCTTGCTTTTTACCAGGATGCTTAAGGGTAAAtt                                                                                               >  2:303275/1‑150 (MQ=255)
                                                         gCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAACCGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGCTGCTGAATGGTAAATTc                                                                                              >  2:175317/1‑150 (MQ=255)
                                                           cAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGATGTATCCAGCCCCTGTTCCGGGGCCGGATCTGCCGGAATAGAATTGCTTTTTACCAGGATGCTTAATTGTAAATTCAg                                                                                            >  2:293189/1‑150 (MQ=255)
                                                             gACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATgg                                                                                                     >  2:205454/1‑139 (MQ=255)
                                                             gACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATgg                                                                                                     <  1:205454/139‑1 (MQ=255)
                                                               cacTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAAt                                                                                        <  2:962224/150‑1 (MQ=255)
                                                               cacTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAAt                                                                                        >  1:1463983/1‑150 (MQ=255)
                                                                acTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAAtt                                                                                       >  2:1474025/1‑150 (MQ=255)
                                                                 cTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAAttt                                                                                      <  2:546835/150‑1 (MQ=255)
                                                                 cTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAAttt                                                                                      <  2:600960/150‑1 (MQ=255)
                                                                  tGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTTCATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTGAATGGTAAATTCAGTAAtttt                                                                                     >  1:338401/1‑149 (MQ=255)
                                                                  tGGGCGCGCAACGGCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGATAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTg                                                                                     <  1:1469396/150‑1 (MQ=255)
                                                                   gggCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTgt                                                                                    <  1:1356885/150‑1 (MQ=255)
                                                                        cgcAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTaa                                                                               >  1:318537/1‑150 (MQ=255)
                                                                          cAACGTCAGCAATTCTGCGTTCGCGTTGTACGGTAAACCGTACATAATTGGACGAGAGGGTTACATCCACTGATACGGGGCAGTATCTGACTCAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAAt                                                                             <  1:293189/150‑1 (MQ=255)
                                                                          cAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAAt                                                                             <  1:1382723/150‑1 (MQ=255)
                                                                                cAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTgg                                                                       <  1:579113/150‑1 (MQ=255)
                                                                                cAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTgg                                                                       <  1:1001125/150‑1 (MQ=255)
                                                                                cAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTAAGTAATATGTAGTAAATTAGTgg                                                                       >  1:1686639/1‑150 (MQ=255)
                                                                                cAGCAAATCAGCTTTCGAGTTTTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTgg                                                                       <  2:868986/150‑1 (MQ=255)
                                                                                  gCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGcc                                                                     <  1:421067/150‑1 (MQ=255)
                                                                                    aaaTCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAg                                                                   <  1:1195177/150‑1 (MQ=255)
                                                                                         tGCTTTCGAGTTGTACTGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGa                                                              <  1:446343/150‑1 (MQ=255)
                                                                                           cTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGATTATACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACa                                                            <  1:1150026/150‑1 (MQ=255)
                                                                                           cTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACa                                                            >  2:1439007/1‑150 (MQ=255)
                                                                                                     gTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGt                                                  >  1:967729/1‑150 (MQ=255)
                                                                                                                 gTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGt                                      >  2:525883/1‑150 (MQ=255)
                                                                                                                  tACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGtt                                     <  2:320410/150‑1 (MQ=255)
                                                                                                                   aCATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTta                                    <  2:1321224/150‑1 (MQ=255)
                                                                                                                     aTAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTtaat                                  <  2:1462616/150‑1 (MQ=255)
                                                                                                                     aTAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTtaat                                  <  2:983559/150‑1 (MQ=255)
                                                                                                                       aaTTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTtaataa                                <  1:91375/150‑1 (MQ=255)
                                                                                                                        aTTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTaataat                               >  1:396122/1‑150 (MQ=255)
                                                                                                                         ttGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATg                              <  1:1319295/150‑1 (MQ=255)
                                                                                                                           ggACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCa                            <  2:1128765/150‑1 (MQ=255)
                                                                                                                             aCGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCaaa                          >  2:523332/1‑150 (MQ=255)
                                                                                                                             aCGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCaaa                          <  1:54400/150‑1 (MQ=255)
                                                                                                                             aCGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCaaa                          <  2:1675302/150‑1 (MQ=255)
                                                                                                                              cGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGTACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAAt                         >  2:19783/1‑150 (MQ=255)
                                                                                                                                agagGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATgc                       >  1:952302/1‑150 (MQ=255)
                                                                                                                                 gagGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATgcg                      >  1:839996/1‑150 (MQ=255)
                                                                                                                                   ggTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGcgcg                    <  1:1662614/150‑1 (MQ=255)
                                                                                                                                    gTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCgg                   >  2:558852/1‑150 (MQ=255)
                                                                                                                                    agaTCCTGCCCCGGAACTGGGGCTGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCgg                   >  1:23766/3‑150 (MQ=255)
                                                                                                                                     gaTCCTGCCCCGGAACTGGGGCTGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGc                  >  2:1369851/2‑150 (MQ=255)
                                                                                                                                      aTCCTGCCCCGGAACTGGGGCTGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCa                 >  1:857879/1‑150 (MQ=255)
                                                                                                                                      aTCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCCGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCa                 >  1:775348/1‑150 (MQ=255)
                                                                                                                                      aTCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCggaa                 >  2:866116/1‑148 (MQ=255)
                                                                                                                                        ccAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAg               >  2:1332848/1‑150 (MQ=255)
                                                                                                                                        ccAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAg               >  2:700890/1‑150 (MQ=255)
                                                                                                                                          aGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGa             >  1:688352/1‑150 (MQ=255)
                                                                                                                                          aGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCAGCTTAATAATGCAAATGCGCGGCAAGGa             >  1:153664/1‑150 (MQ=255)
                                                                                                                                            ccccAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATa           >  1:573005/1‑150 (MQ=255)
                                                                                                                                            ccccAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATa           >  2:1503454/1‑150 (MQ=255)
                                                                                                                                              ccAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATTCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAgt         <  1:1065224/150‑2 (MQ=255)
                                                                                                                                              ccAGGTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGc         <  1:1328384/150‑1 (MQ=255)
                                                                                                                                               catttCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCa        <  2:488539/147‑1 (MQ=255)
                                                                                                                                                     cGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGt  <  1:1558748/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGT  >  NC_000913/4256225‑4256523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑