Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,256,380 A→T 5.9% P29P (CCT→CCA)  plsB ← glycerol‑3‑phosphate O‑acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,256,3800AT 5.9% 403.7 / 10.5 136P29P (CCT→CCA) plsBglycerol‑3‑phosphate O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (62/64);  new base T (4/4);  total (68/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.69e-01

Only 100 of 139 total aligned reads displayed.

AGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGG  >  NC_000913/4256231‑4256528
                                                                                                                                                     |                                                                                                                                                    
aGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCa                                                                                                                                                      <  2:569054/150‑1 (MQ=255)
aGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCa                                                                                                                                                      <  1:1275030/150‑1 (MQ=255)
 gTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCCTGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAg                                                                                                                                                     <  1:1190920/150‑1 (MQ=255)
   aGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGa                                                                                                                                                   <  1:479262/150‑1 (MQ=255)
   aGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGa                                                                                                                                                   >  1:600960/1‑150 (MQ=255)
    gCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCGGAACTGGGGCTGGAt                                                                                                                                                  <  2:816917/150‑1 (MQ=255)
    gCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGAt                                                                                                                                                  >  1:578531/1‑150 (MQ=255)
      gTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGCGTTGTACGTTAAAACGTACATAATTTGACGAGAGGTATCCAGCCCCAGTTCAGGGGAAGTATCt                                                                                                                                                >  1:1275651/1‑150 (MQ=255)
      gTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCTGCCCCGGAACTGGGGCTGGAtac                                                                                                                                                <  2:934084/150‑3 (MQ=255)
       tGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCTGCCCCGGAACTGGGGCTGGAtacc                                                                                                                                               <  2:902173/150‑4 (MQ=255)
         ccGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCTGCCCCGGAACTGGGGCTGGAtacctc                                                                                                                                             <  1:724179/150‑6 (MQ=255)
          cGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCg                                                                                                                                            >  2:44253/1‑150 (MQ=255)
          cGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCg                                                                                                                                            <  2:581622/150‑1 (MQ=255)
              gATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAAt                                                                                                                                        >  2:710/1‑150 (MQ=255)
                    cAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACtt                                                                                                                                  >  2:419592/1‑150 (MQ=255)
                        ggCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCtt                                                                                                                              <  2:800927/150‑1 (MQ=255)
                            ctAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCtttttt                                                                                                                          >  1:1100420/1‑149 (MQ=255)
                            ctAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTa                                                                                                                          >  1:368192/1‑150 (MQ=255)
                            ctAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTa                                                                                                                          <  1:1656701/150‑1 (MQ=255)
                            ctAACGGGTCAGGCAAGTCATGAGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTa                                                                                                                          <  1:1057391/150‑1 (MQ=255)
                            ctAACGGGACAGGCAAGTCATGTGCCAGACACTGGGCCCGCAACGTCAGCAAAACTGCTTTCGAGTTGTACGGGTTCACGTGCATTATTGGATGAGAGGTTTCCTGCCCCAGTTCCGGGGCCGGATCTGCGGGAATAGACTTGcagtgta                                                                                                                          >  2:328613/1‑144 (MQ=255)
                            ctAACGCGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGTAATAGACTTGCTTTTTa                                                                                                                          <  1:895392/150‑1 (MQ=255)
                             tAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTAc                                                                                                                         <  2:1311891/150‑1 (MQ=255)
                             tAACGGGGCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTAc                                                                                                                         <  1:1139844/150‑1 (MQ=255)
                             aaaCGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCGTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTAc                                                                                                                         <  1:838573/149‑1 (MQ=255)
                               aCGGGTCCGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGAAAAACGTACATAATTGGACGATAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCa                                                                                                                       <  1:480283/150‑1 (MQ=255)
                               aCGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCa                                                                                                                       >  1:61567/1‑150 (MQ=255)
                                cGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAg                                                                                                                      >  1:1083189/1‑150 (MQ=255)
                                       ggCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAACCGTTCCTAATTGGGCGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGAACTGGCGGATTAGCCTTGGTTTTTTCCTGGATGGtt                                                                                                               >  2:1146551/1‑150 (MQ=255)
                                        gCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTa                                                                                                              >  1:870584/1‑150 (MQ=255)
                                        gCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTa                                                                                                              >  1:983559/1‑150 (MQ=255)
                                         cAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTaa                                                                                                             >  2:91375/1‑150 (MQ=255)
                                            gTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGGGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATgg                                                                                                          >  1:784393/1‑150 (MQ=255)
                                            gTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATgg                                                                                                          >  1:546835/1‑150 (MQ=255)
                                            gTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATgg                                                                                                          >  1:1255890/1‑150 (MQ=255)
                                             tCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGt                                                                                                         <  2:1446608/150‑1 (MQ=255)
                                              cATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTa                                                                                                        <  1:857556/150‑1 (MQ=255)
                                               aTGTTCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACTAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTaa                                                                                                       <  1:1563032/150‑1 (MQ=255)
                                                tgtgCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTaaa                                                                                                      <  2:772845/150‑1 (MQ=255)
                                                  tgCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGCCTTGCTTTTTACCAGGATGCTTAAGGGTAAAtt                                                                                                    >  2:303275/1‑150 (MQ=255)
                                                   gCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTc                                                                                                   >  1:327803/1‑150 (MQ=255)
                                                     cAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGATGTATCCAGCCCCTGTTCCGGGGCCGGATCTGCCGGAATAGAATTGCTTTTTACCAGGATGCTTAATTGTAAATTCAg                                                                                                 >  2:293189/1‑150 (MQ=255)
                                                      aGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTTGACGAGAGGTATCCATCCCCAGTTCCGGGGCAGGATCTGCCGTAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGt                                                                                                <  1:857283/150‑1 (MQ=255)
                                                       gACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATgg                                                                                                          >  2:205454/1‑139 (MQ=255)
                                                       gACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATgg                                                                                                          <  1:205454/139‑1 (MQ=255)
                                                         cacTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAAt                                                                                             >  1:1463983/1‑150 (MQ=255)
                                                         cacTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAAt                                                                                             <  2:962224/150‑1 (MQ=255)
                                                          acTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAAtt                                                                                            >  2:1474025/1‑150 (MQ=255)
                                                           cTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAAttt                                                                                           <  2:600960/150‑1 (MQ=255)
                                                            tGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTTCATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTGAATGGTAAATTCAGTAAtttt                                                                                          >  1:338401/1‑149 (MQ=255)
                                                            tGGGCGCGCAACGGCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGATAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTg                                                                                          <  1:1469396/150‑1 (MQ=255)
                                                             gggCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTgt                                                                                         <  1:1356885/150‑1 (MQ=255)
                                                                  cgcAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTaa                                                                                    >  2:1526989/1‑150 (MQ=255)
                                                                    cAACGTCAGCAATTCTGCGTTCGCGTTGTACGGTAAACCGTACATAATTGGACGAGAGGGTTACATCCACTGATACGGGGCAGTATCTGACTCAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAAt                                                                                  <  1:293189/150‑1 (MQ=255)
                                                                    cAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAAt                                                                                  <  1:1382723/150‑1 (MQ=255)
                                                                          cAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTgg                                                                            <  1:1001125/150‑1 (MQ=255)
                                                                          cAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTAAGTAATATGTAGTAAATTAGTgg                                                                            >  1:1686639/1‑150 (MQ=255)
                                                                          cAGCAAATCAGCTTTCGAGTTTTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTgg                                                                            <  2:868986/150‑1 (MQ=255)
                                                                            gCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGcc                                                                          <  1:421067/150‑1 (MQ=255)
                                                                            gCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTAAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGcc                                                                          <  2:1374434/150‑1 (MQ=255)
                                                                              aaaTCTGCTTTCGAGTTGTTCGGTAAAAAGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGGAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAg                                                                        <  1:914258/150‑1 (MQ=255)
                                                                              aaaTCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAg                                                                        <  1:1195177/150‑1 (MQ=255)
                                                                                 tCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTGAATTCGTGGCCAGCCg                                                                     <  1:1691691/150‑1 (MQ=255)
                                                                                   tGCTTTCGAGTTGTACTGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGa                                                                   <  1:446343/150‑1 (MQ=255)
                                                                                    gCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGAc                                                                  <  2:1080583/150‑1 (MQ=255)
                                                                                     cTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGATTATACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACa                                                                 <  1:1150026/150‑1 (MQ=255)
                                                                                     cTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACa                                                                 >  1:156965/1‑150 (MQ=255)
                                                                                     cTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACa                                                                 >  1:1475479/1‑150 (MQ=255)
                                                                                                tACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTa                                                      <  1:710/150‑1 (MQ=255)
                                                                                                   ggTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAg                                                   >  1:250792/1‑150 (MQ=255)
                                                                                                     tAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGcc                                                 <  1:1105485/150‑1 (MQ=255)
                                                                                                           gTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGt                                           >  2:525883/1‑150 (MQ=255)
                                                                                                             aCATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTta                                         <  2:1321224/150‑1 (MQ=255)
                                                                                                               aTAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTtaat                                       <  2:983559/150‑1 (MQ=255)
                                                                                                               aTAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTtaat                                       <  2:1462616/150‑1 (MQ=255)
                                                                                                                 aaTTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTtaataa                                     <  1:91375/150‑1 (MQ=255)
                                                                                                                  aTTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTaataat                                    >  1:396122/1‑150 (MQ=255)
                                                                                                                   ttGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATg                                   <  1:1319295/150‑1 (MQ=255)
                                                                                                                       aCGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCaaa                               <  2:1675302/150‑1 (MQ=255)
                                                                                                                       aCGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCaaa                               >  2:523332/1‑150 (MQ=255)
                                                                                                                        cGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGTACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAAt                              >  2:19783/1‑150 (MQ=255)
                                                                                                                          agagGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATgc                            >  1:952302/1‑150 (MQ=255)
                                                                                                                             ggTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGcgcg                         <  1:1662614/150‑1 (MQ=255)
                                                                                                                              gTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCgg                        >  2:558852/1‑150 (MQ=255)
                                                                                                                               gaTCCTGCCCCGGAACTGGGGCTGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGc                       >  2:1369851/2‑150 (MQ=255)
                                                                                                                                aTCCTGCCCCGGAACTGGGGCTGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCa                      >  1:857879/1‑150 (MQ=255)
                                                                                                                                aTCCTGCCCCGGAACTGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCa                      >  2:18375/1‑150 (MQ=255)
                                                                                                                                aTCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCggaa                      >  2:866116/1‑148 (MQ=255)
                                                                                                                                  ccAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAg                    >  2:700890/1‑150 (MQ=255)
                                                                                                                                  ccAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAg                    >  2:1332848/1‑150 (MQ=255)
                                                                                                                                      ccccAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATa                >  2:1503454/1‑150 (MQ=255)
                                                                                                                                      ccccAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATa                >  1:573005/1‑150 (MQ=255)
                                                                                                                                        ccAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATTCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAgt              <  1:1065224/150‑2 (MQ=255)
                                                                                                                                        ccAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGc              <  2:543364/150‑1 (MQ=255)
                                                                                                                                        ccAGGTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGc              <  1:1328384/150‑1 (MQ=255)
                                                                                                                                         cggaactggggCTGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCa             >  1:1329344/8‑150 (MQ=255)
                                                                                                                                               cGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGt       <  1:1558748/150‑1 (MQ=255)
                                                                                                                                                 gggCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGAGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCa     >  2:1650490/1‑150 (MQ=255)
                                                                                                                                                  ggCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCAt    >  1:737086/1‑150 (MQ=255)
                                                                                                                                                   gCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATg   <  2:61567/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGG  >  NC_000913/4256231‑4256528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑