Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,261,358 T→A 6.2% intergenic (+52/‑311) yjbM → / → dusA uncharacterized protein/tRNA‑dihydrouridine synthase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,261,3580TA 6.2% 542.2 / 16.0 178intergenic (+52/‑311)yjbM/dusAuncharacterized protein/tRNA‑dihydrouridine synthase A
Reads supporting (aligned to +/- strand):  ref base T (83/84);  new base A (3/8);  total (86/92)
Fisher's exact test for biased strand distribution p-value = 2.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

Only 100 of 183 total aligned reads displayed.

CGAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTCCATCA  >  NC_000913/4261209‑4261504
                                                                                                                                                     |                                                                                                                                                  
cgATGTCGTTTGACCCAACCTCAAAGGAAGAGATTAAAATAATTAAAGACACTTCTAAATAAAATTATTATTTCATATTGGTTAAAACGGAGAAATAATATGTGAAGTTCATCGATAGTATGGAAGGCATTATATAAAAGGACCCAatat                                                                                                                                                    >  2:268323/1‑150 (MQ=255)
     tCGTTTGACCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAAtatttatt                                                                                                                                               <  2:565110/150‑1 (MQ=255)
         ttGACCCAACCTCAAAGGAAGAGATTAAAATACTCACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGt                                                                                                                                           <  2:1181382/150‑1 (MQ=255)
          tGACCCAACCTCAAAGTAAGAGATTAAAATACTTACAGACACTTCAAAATTAATTTATGATTTGTTCTGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATAAATATTGGGtc                                                                                                                                          <  1:145793/150‑2 (MQ=255)
          tGACCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGtt                                                                                                                                          >  1:1271064/1‑150 (MQ=255)
           taCCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTc                                                                                                                                         <  1:1428729/149‑1 (MQ=255)
           gACCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTc                                                                                                                                         <  2:580616/150‑1 (MQ=255)
           gACCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTc                                                                                                                                         >  2:965683/1‑150 (MQ=255)
            aCCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCt                                                                                                                                        >  1:291424/1‑150 (MQ=255)
              ccAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCttt                                                                                                                                      >  2:1506318/1‑150 (MQ=255)
              ccAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGAACCCAATAAATATTGGGTCCttt                                                                                                                                      <  2:1486594/150‑1 (MQ=255)
              ccAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTAAATAAAAGAACCCAATAAATATTGGGTCCttt                                                                                                                                      <  1:1455499/150‑1 (MQ=255)
               cAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGAACCCAATAAATATTGGGTCCtttt                                                                                                                                     <  2:1298040/150‑1 (MQ=255)
                aaCCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCAAATATTTATTGGGTTCttttt                                                                                                                                    >  1:1239004/1‑150 (MQ=255)
                  ccTCCAATGAAGAGATTAAACTACTTACAGACAATTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGAACCCAATAAATATTGGGTCCttttata                                                                                                                                  <  1:811602/150‑4 (MQ=255)
                   cTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTtct                                                                                                                                 <  1:1389873/150‑1 (MQ=255)
                     cAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTctct                                                                                                                               >  2:878042/1‑150 (MQ=255)
                       aaGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTAt                                                                                                                             <  2:755327/150‑1 (MQ=255)
                         ggAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCa                                                                                                                           >  1:1463824/1‑150 (MQ=255)
                          gAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTATTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGTGTTCTTTTTTCTCTATCaa                                                                                                                          >  1:80795/1‑150 (MQ=255)
                             gagaTTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATAc                                                                                                                       <  2:902691/150‑1 (MQ=255)
                              agaTTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGTATAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACt                                                                                                                      <  1:1467125/150‑1 (MQ=255)
                              agaTTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACt                                                                                                                      >  1:660763/1‑150 (MQ=255)
                               gaTTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTa                                                                                                                     >  1:510280/1‑150 (MQ=255)
                                  tAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTa                                                                                                                  <  2:1700816/150‑1 (MQ=255)
                                    aaaTACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGc                                                                                                                >  1:1315108/1‑150 (MQ=255)
                                    aaaTACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATAAATATTGGGtcc                                                                                                                                         <  2:609009/125‑3 (MQ=255)
                                    aaaTACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATAAATATTGGGtcc                                                                                                                                         >  1:609009/1‑123 (MQ=255)
                                         cTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGga                                                                                                           >  1:565775/1‑150 (MQ=255)
                                          ttACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGgag                                                                                                          <  2:1172108/150‑1 (MQ=255)
                                             cAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAACGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGata                                                                                                       <  2:1402907/150‑1 (MQ=255)
                                               gACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGGGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGatata                                                                                                     <  2:90386/150‑1 (MQ=255)
                                               gACACTTCTAAATTAATTTATGATTTGTTCGGGGGTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGatata                                                                                                     <  2:324047/150‑1 (MQ=255)
                                                 cacTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGatatata                                                                                                   >  2:1142730/1‑149 (MQ=255)
                                                 aacTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGATATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATc                                                                                                   <  2:1623049/149‑1 (MQ=255)
                                                  acTTCTAAATTAATTTATGACTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCa                                                                                                  <  1:1373632/150‑1 (MQ=255)
                                                   cTTCTAGATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCAc                                                                                                 >  1:1717949/1‑150 (MQ=255)
                                                   cTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCAc                                                                                                 >  1:1133592/1‑150 (MQ=255)
                                                    ttCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCAcc                                                                                                <  2:1133592/150‑1 (MQ=255)
                                                    ttCTAAAATAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCAcc                                                                                                <  1:1161885/150‑1 (MQ=255)
                                                     tCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCa                                                                                               >  2:861564/1‑150 (MQ=255)
                                                      cTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCag                                                                                              >  2:545725/1‑150 (MQ=255)
                                                      cTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCag                                                                                              >  1:202992/1‑150 (MQ=255)
                                                      atAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCag                                                                                              <  2:1265934/149‑1 (MQ=255)
                                                        aaaTTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCagag                                                                                            >  2:485856/1‑150 (MQ=255)
                                                          attaattTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGtt                                                                                          >  1:379567/1‑150 (MQ=255)
                                                              attTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAAt                                                                                      <  1:120596/150‑1 (MQ=255)
                                                               tttATGATTTGTTCGGTGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGTCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAAtg                                                                                     <  1:765206/150‑1 (MQ=255)
                                                               tttATGATTTGTTCGGGGTAAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGTCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAAtg                                                                                     <  1:413003/150‑1 (MQ=255)
                                                                ttATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAAtgt                                                                                    <  1:1680613/150‑1 (MQ=255)
                                                                 tATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAAtgtg                                                                                   <  2:996765/150‑1 (MQ=255)
                                                                       ttGTTCGGGGTTAAATCGGAGAAATAATTTGTGAAGTTCTTCGATAGTATTGAAGGCATTTTATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGAttt                                                                             <  2:1717621/150‑1 (MQ=255)
                                                                            ctgggTTAAATCGTAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTtttat                                                                        <  1:1080017/148‑1 (MQ=255)
                                                                             ggggTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTtttatt                                                                       >  2:772430/1‑150 (MQ=255)
                                                                               ggTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGAAGGGAGATATATCACCAGAGTTTAATGTGTGATTTttttttta                                                                     >  2:62858/1‑150 (MQ=255)
                                                                                 ttAAATCGGAGAAATAATATGTGAATTTCTTAGATAGTTTGGAAGGCATTATATAAATTGACCCAATATTTATTTGGTTCTTTTTTCTCTATCAATACTATTAGCAGTGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTAtc                                                                   <  1:6811/150‑1 (MQ=255)
                                                                                  tAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTAtcg                                                                  >  1:1432666/1‑150 (MQ=255)
                                                                                      tCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACAAGAGTTTAATGTGTGATTTTTTATTTATCGTCGa                                                              >  2:388974/1‑150 (MQ=255)
                                                                                        ggAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCTGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATAGTCGAAc                                                            >  2:831221/1‑150 (MQ=255)
                                                                                        ggAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAAc                                                            >  2:255984/1‑150 (MQ=255)
                                                                                        ggAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAAc                                                            >  2:1417408/1‑150 (MQ=255)
                                                                                           taaaTAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTg                                                         <  1:708603/149‑1 (MQ=255)
                                                                                            aaaTAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTgg                                                        >  2:950824/1‑150 (MQ=255)
                                                                                            aaaTAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTgg                                                        >  2:590111/1‑150 (MQ=255)
                                                                                                aataTGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTg                                                    <  1:1506318/150‑1 (MQ=255)
                                                                                                aataTGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTg                                                    <  2:1374308/150‑1 (MQ=255)
                                                                                                aataTGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTg                                                    >  2:31582/1‑150 (MQ=255)
                                                                                                aataTGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTg                                                    <  1:402100/150‑1 (MQ=255)
                                                                                                aataTGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGAAGGGAGATATATCACCAGAGTTTAATTTGTGATTTTTTATTTATCGTCGAACATGGAttt                                                    >  1:1696233/1‑149 (MQ=255)
                                                                                                   atGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGttt                                                 <  2:671981/150‑1 (MQ=255)
                                                                                                      tgAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATc                                              >  1:524869/1‑150 (MQ=255)
                                                                                                        aaGTTCTTCGAAAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCAt                                            <  2:1603328/150‑1 (MQ=255)
                                                                                                            tcttcGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGc                                        <  2:1385443/150‑1 (MQ=255)
                                                                                                             cttcGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGcc                                       <  1:637906/150‑1 (MQ=255)
                                                                                                             cttcGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGcc                                       >  1:161762/1‑150 (MQ=255)
                                                                                                             cttcGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGcc                                       >  1:259802/1‑150 (MQ=255)
                                                                                                             cttcGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGcc                                       >  1:863833/1‑150 (MQ=255)
                                                                                                             attcGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGcc                                       <  1:1590768/149‑1 (MQ=255)
                                                                                                              ttcGATAGTATGGAATGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCt                                      <  1:1156956/150‑1 (MQ=255)
                                                                                                                cGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATAATTGGCCTTa                                    >  2:1326646/1‑150 (MQ=255)
                                                                                                                cGATAGTATGGAAGGCATTATATAAAAGGACCCAAAATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTAGAACCTGGATTGTTTATCATTGGCCTTa                                    >  1:537701/1‑150 (MQ=255)
                                                                                                                  aTAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAAc                                  <  1:965683/150‑1 (MQ=255)
                                                                                                                     gTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGACGAACCTCTATTGTTTATCATTGGCCTTAACaaa                               >  2:201875/1‑150 (MQ=255)
                                                                                                                      tATGGAATGCATTATATAAAAGTACCCAATATTTATTGTGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTTGATTGTTTATCATTGGCCTTAACAAAg                              <  1:904805/150‑1 (MQ=255)
                                                                                                                      tATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACaaat                              >  2:424511/1‑149 (MQ=255)
                                                                                                                      tATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAg                              <  1:34022/150‑1 (MQ=255)
                                                                                                                      tATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAg                              <  2:402970/150‑1 (MQ=255)
                                                                                                                       aTGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGt                             >  1:622841/1‑150 (MQ=255)
                                                                                                                         ggAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTa                           >  1:1488832/1‑150 (MQ=255)
                                                                                                                          gAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTaa                          >  2:25901/1‑150 (MQ=255)
                                                                                                                            ttgCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTATCTATCAATACTATTAGAAGGTAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACg                        <  2:820020/148‑1 (MQ=255)
                                                                                                                            aGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACg                        >  2:799568/1‑150 (MQ=255)
                                                                                                                                aTTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAGTGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCta                    >  2:1105145/1‑150 (MQ=255)
                                                                                                                                    tataAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCtaataa                <  1:453905/150‑1 (MQ=255)
                                                                                                                                    tataAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCtaataa                >  1:1558611/1‑150 (MQ=255)
                                                                                                                                       aaaaGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGat             >  2:92720/1‑150 (MQ=255)
                                                                                                                                        aaaGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGatt            <  2:1689687/150‑1 (MQ=255)
                                                                                                                                          agaaCCCAATAAATATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATAGTCGAACCTGGATTGTTTATCATTTGCCTTAACAAAGTTAACGGCTAATAAGattat          >  2:1051938/4‑150 (MQ=255)
                                                                                                                                              cccAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTcc      <  2:524869/150‑1 (MQ=255)
                                                                                                                                                  atatTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTCCATCa  <  1:101818/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CGAAGTCGTTTGACCCAACCTCAAAGGAAGAGATTAAAATACTTACAGACACTTCTAAATTAATTTATGATTTGTTCGGGGTTAAATCGGAGAAATAATATGTGAAGTTCTTCGATAGTATGGAAGGCATTATATAAAAGGACCCAATATTTATTGGGTTCTTTTTTCTCTATCAATACTATTAGCAGGGAGATATATCACCAGAGTTTAATGTGTGATTTTTTATTTATCGTCGAACCTGGATTGTTTATCATTGGCCTTAACAAAGTTAACGGCTAATAAGATTATTTCCATCA  >  NC_000913/4261209‑4261504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: