Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,270,146 T→C 8.5% intergenic (+19/‑92) aphA → / → yjbQ acid phosphatase/phosphotransferase, class B, non‑specific/thiamine phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,270,1460TC 8.5% 327.6 / 17.3 118intergenic (+19/‑92)aphA/yjbQacid phosphatase/phosphotransferase, class B, non‑specific/thiamine phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (52/54);  new base C (6/4);  total (59/59)
Fisher's exact test for biased strand distribution p-value = 7.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01

Only 100 of 120 total aligned reads displayed.

ATGATATTACCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAA  >  NC_000913/4269997‑4270295
                                                                                                                                                     |                                                                                                                                                     
aTGATATTACCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGAt                                                                                                                                                       >  2:191169/1‑150 (MQ=255)
  gATATTACCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGc                                                                                                                                                     <  1:149904/150‑1 (MQ=255)
  gATATTACCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGGTTGGTGAAGCGGTGCTCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGc                                                                                                                                                     >  1:256802/1‑150 (MQ=255)
    tatTACCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGGCAATTCAGACTACTGACAGAGCGGGAGCGCGTGATGctc                                                                                                                                                   >  2:856494/1‑150 (MQ=255)
        aCCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCATCACGCTCTccc                                                                                                                                               <  2:566783/150‑1 (MQ=255)
         ccgccgCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCTCAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCATCACGCTCTCCCg                                                                                                                                              <  1:263385/150‑1 (MQ=255)
         ccgccgCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCg                                                                                                                                              <  1:1423718/150‑1 (MQ=255)
         ccgccgCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCATCACGCTCTCCCg                                                                                                                                              <  2:707644/150‑1 (MQ=255)
           gccgcACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGaa                                                                                                                                            >  2:341768/1‑150 (MQ=255)
             cgcACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATTCTGACAGAGCGGGAGAGCATCACGCTCTCCCgctct                                                                                                                                          <  2:428294/150‑5 (MQ=255)
                aCGCGCTGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTg                                                                                                                                       <  2:1470475/150‑1 (MQ=255)
                 cgcgATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCTGAATACTGACAGAGCGGGATAGCGTGATGCTCTCCCGAATGCTGt                                                                                                                                      <  1:1393583/150‑1 (MQ=255)
                   cgATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGttt                                                                                                                                    >  2:1457026/1‑150 (MQ=255)
                    gATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGtttt                                                                                                                                   >  1:1463602/1‑150 (MQ=255)
                       gTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGttttttt                                                                                                                                <  1:972672/150‑1 (MQ=255)
                         cGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGCGGGCGTTGGGTGAAGAGGTGATCGTCTATTCAGAAGACTGCCAGAGCGGCAGAGCGTGAGGCGCTACCGAATGCTGTATTTATaa                                                                                                                              >  1:1522907/1‑150 (MQ=255)
                           gcgcgcGTGGTATCCGCATTCTGCGCGCCTCCAACTCAACCTACAAACCCTTGCCACAAGCGGGGGCGTTTGGTGAAGAGGTGATCGTCAAATCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATc                                                                                                                            <  1:867313/150‑1 (MQ=255)
                           gcgcTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTCTTTTTAATc                                                                                                                            <  2:1314978/150‑1 (MQ=255)
                             gcTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCGTTGCCACAAGCGGGTGCGTTTGGGGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATcac                                                                                                                          <  2:911780/150‑1 (MQ=255)
                             gcTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCATTTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATcac                                                                                                                          >  2:859046/1‑150 (MQ=255)
                              cTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATcacc                                                                                                                         >  1:1493621/1‑149 (MQ=255)
                                  tGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCtt                                                                                                                     >  2:704400/1‑150 (MQ=255)
                                  tGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCtt                                                                                                                     >  2:1645827/1‑150 (MQ=255)
                                      aTCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATc                                                                                                                 >  2:1237815/1‑150 (MQ=255)
                                      aTCCGCATTCTGCGCGCCTCCAACTCAACCTACAAACCCTTTCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATc                                                                                                                 <  2:907881/150‑1 (MQ=255)
                                       tCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGCTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCAGTTTTTTTAATCACACCTTTATcc                                                                                                                >  1:1645275/1‑150 (MQ=255)
                                        ccGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCt                                                                                                               >  2:912555/1‑150 (MQ=255)
                                        ccGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCt                                                                                                               >  1:1372946/1‑150 (MQ=255)
                                           cATTCTGCGCGCCTCCCACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTc                                                                                                            >  1:1426553/1‑150 (MQ=255)
                                           cATTCTGCGCGCCTCCAACTCTCACTACAACCCCTTGCCACAAGCAGGGGCGTTTGGGGCAGAGCTGCTCGTCTATTCAGAATACTGACAGAGCGGGAGAGCGGGATGCTCTCCCGAATGCTGTCTTTTTAATCACACCTTTAACCTTTc                                                                                                            <  2:1093382/150‑1 (MQ=255)
                                                  ggcgcCTCCAACTCTAACTACAACCCCTTGCCACACGCGGGTGCGGTTGGTGAAGTGGGGAGCGCCAATCCAGAATACTGACAGCGCGGGAGAGCGTGTGGCTCTCCGGAATGCTTTTTTTTTAATCACACCTTTATCCTTGCGCTGTCt                                                                                                     <  2:1150920/149‑1 (MQ=255)
                                                  cgcgcCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCt                                                                                                     <  1:489861/150‑1 (MQ=255)
                                                  cgcgcCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCt                                                                                                     >  1:1028164/1‑150 (MQ=255)
                                                      ccTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTtgct                                                                                                 >  2:686695/1‑150 (MQ=255)
                                                       cTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCATAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTtgctg                                                                                                <  1:1270145/150‑1 (MQ=255)
                                                            aCTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGTTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAAc                                                                                           <  2:1277919/150‑1 (MQ=255)
                                                             ctctACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACt                                                                                          >  2:992778/1‑150 (MQ=255)
                                                              tctACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTg                                                                                         >  2:1117350/1‑150 (MQ=255)
                                                               ctacctacAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGa                                                                                        >  2:66727/1‑150 (MQ=255)
                                                                 acctacAAACCCTTGCCACCAGCGGGTGCGTTTGGGGAAGAGGTGATCGTCAATTCAGAATACTCACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGAtt                                                                                      <  1:306739/150‑1 (MQ=255)
                                                                 acctacAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGGGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGAtt                                                                                      <  2:150068/150‑1 (MQ=255)
                                                                 acctacAAAACCTTGCCTCAAGCGGGTGCGTTGGGTGAAGAGGGGATCGTCAATTCAGAATACTGACAGAGCGGGCGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGAtt                                                                                      <  2:62432/150‑1 (MQ=255)
                                                                  cctccAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTa                                                                                     <  1:1577243/150‑1 (MQ=255)
                                                                    tacAAACCGTTGCCACAAGCGGGTGGGTTTGGTGATGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAag                                                                                   <  2:1460997/150‑1 (MQ=255)
                                                                    tacAAACCCTTGCCACAAGCGGGGGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAag                                                                                   <  1:39840/150‑1 (MQ=255)
                                                                      cAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAagag                                                                                 <  1:192586/150‑1 (MQ=255)
                                                                      cAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAagag                                                                                 <  1:675459/150‑1 (MQ=255)
                                                                       aaaCCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAagaga                                                                                <  1:96794/150‑1 (MQ=255)
                                                                       aaaCCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAagaga                                                                                >  2:1158420/1‑150 (MQ=255)
                                                                        aaCCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAagagag                                                                               >  2:928544/1‑150 (MQ=255)
                                                                            cTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGtttt                                                                           >  1:1557077/1‑150 (MQ=255)
                                                                             ttGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTa                                                                          <  2:1649660/150‑1 (MQ=255)
                                                                                 cacaAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATcga                                                                      >  2:1540762/1‑148 (MQ=255)
                                                                                 cacaAGCGGGTGCGTATGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTAATCATTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCaa                                                                      >  1:68066/1‑150 (MQ=255)
                                                                                   caAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAgg                                                                    <  1:1262858/150‑1 (MQ=255)
                                                                                    aaGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGa                                                                   <  2:712564/150‑1 (MQ=255)
                                                                                     aGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGag                                                                  >  2:1717081/1‑150 (MQ=255)
                                                                                      gCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGagc                                                                 <  1:1717081/150‑1 (MQ=255)
                                                                                       cGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGagca                                                                >  2:1391984/1‑150 (MQ=255)
                                                                                         ggTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGagcagc                                                              >  1:825079/1‑150 (MQ=255)
                                                                                              gTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACAtg                                                         >  1:450007/1‑150 (MQ=255)
                                                                                              gTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACAtg                                                         >  1:629301/1‑150 (MQ=255)
                                                                                               tttGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACAtgt                                                        <  1:217622/150‑1 (MQ=255)
                                                                                                ttGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACAtgtg                                                       >  1:1504209/1‑150 (MQ=255)
                                                                                                 tGGTGCAGAGGGGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTgg                                                      <  1:1563128/150‑1 (MQ=255)
                                                                                                 tGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTgg                                                      <  1:407215/150‑1 (MQ=255)
                                                                                                 tGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTgg                                                      <  1:1662574/150‑1 (MQ=255)
                                                                                                 tGGGGAAGAGGTGAGCGTCAATTCTGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTgg                                                      <  1:1570176/150‑1 (MQ=255)
                                                                                                     gAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATc                                                  >  2:805792/1‑150 (MQ=255)
                                                                                                     gAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATACTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCCCATGTGGTATc                                                  >  1:324102/1‑150 (MQ=255)
                                                                                                     gAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGAGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATc                                                  >  1:1214044/1‑150 (MQ=255)
                                                                                                       agagGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCaa                                                >  2:744261/1‑150 (MQ=255)
                                                                                                         agGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCaaaa                                              <  1:341768/150‑1 (MQ=255)
                                                                                                             gATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACg                                          >  2:1602068/1‑150 (MQ=255)
                                                                                                              aTCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGc                                         <  2:62684/150‑1 (MQ=255)
                                                                                                               tCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCt                                        >  1:103654/1‑150 (MQ=255)
                                                                                                                 gTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCa                                      >  2:520497/1‑150 (MQ=255)
                                                                                                                          gAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAgcg                             <  1:1645827/150‑1 (MQ=255)
                                                                                                                               cTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGAAGATCACGCTTAGCGCCaaa                        >  2:1661246/1‑150 (MQ=255)
                                                                                                                                 cagcattcGGGAGAGCATCACGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAAtc                      >  1:666909/8‑150 (MQ=255)
                                                                                                                                   cAGATCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTCTCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAAtctc                    <  2:276944/150‑1 (MQ=255)
                                                                                                                                   cAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAAtctc                    >  1:520669/1‑150 (MQ=255)
                                                                                                                                   cAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCAGCAAACTGATTAAGAGAGTTTTATAAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAAtctc                    >  2:1540631/1‑150 (MQ=255)
                                                                                                                                    cattcGGGAGAGCATCACGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGTAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCg                   >  1:640695/5‑150 (MQ=255)
                                                                                                                                    agagCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCg                   <  2:1604591/150‑1 (MQ=255)
                                                                                                                                    agagCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCg                   <  1:1371318/150‑1 (MQ=255)
                                                                                                                                     attcGGGAGAGCATCACGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGt                  >  1:1625586/4‑150 (MQ=255)
                                                                                                                                     attcGGGAGAGCATCACGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGt                  >  2:1505398/4‑150 (MQ=255)
                                                                                                                                     attcGGGAGAGCATCACGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGt                  >  1:432359/4‑150 (MQ=255)
                                                                                                                                      agCGGGAGAGCGTGATGCTCGCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTTGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTg                 <  2:845830/150‑1 (MQ=255)
                                                                                                                                        cGGGTGAGCGTGATGCTCTACCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGATAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTggg               <  1:224964/150‑1 (MQ=255)
                                                                                                                                          ggAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTATTCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTTTCAAAAGACGCTCCCGCTTTGCGCAAAATCTCGTGGGtt             >  2:340653/1‑150 (MQ=255)
                                                                                                                                          ggAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGtt             >  2:297854/1‑150 (MQ=255)
                                                                                                                                          ggAGAGCGCGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGtt             <  2:1214044/150‑1 (MQ=255)
                                                                                                                                          ggAGAGCATCACGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGtt             >  2:1578004/1‑150 (MQ=255)
                                                                                                                                               gCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATc        >  1:442019/1‑150 (MQ=255)
                                                                                                                                                 gTGATGCTCTCCCGAATGCTGAATTTTTAATCACACCATTATCCTTTCGCAGTCTTGCTGCAAACTGATTAAGAGACTTTTATAAAGGAAAAGCACATTTAGTATCAAAAGACACTCACTCTAAGCGACAAATCTCGTGCGATTCATCTg      >  2:273624/1‑150 (MQ=255)
                                                                                                                                                  tGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTgg     <  2:1032172/150‑1 (MQ=255)
                                                                                                                                                    aTGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTa   <  1:912555/150‑1 (MQ=255)
                                                                                                                                                    aTGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTa   >  1:1599523/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATGATATTACCGCCGCACGCGATGTCGGCGCTCGTGGTATCCGCATTCTGCGCGCCTCCAACTCTACCTACAAACCCTTGCCACAAGCGGGTGCGTTTGGTGAAGAGGTGATCGTCAATTCAGAATACTGACAGAGCGGGAGAGCGTGATGCTCTCCCGAATGCTGTTTTTTTAATCACACCTTTATCCTTTCGCTGTCTTGCTGCAAACTGATTAAGAGAGTTTTATCAAGGAGCAGCACATGTGGTATCAAAAGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAA  >  NC_000913/4269997‑4270295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: