Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,277,942 A→T 7.9% noncoding (125/140 nt) ryjA ← novel sRNA, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,277,9420AT 7.9% 389.3 / 16.7 144noncoding (125/140 nt)ryjAnovel sRNA, function unknown
Reads supporting (aligned to +/- strand):  ref base A (66/65);  new base T (3/9);  total (69/75)
Fisher's exact test for biased strand distribution p-value = 1.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01

Only 100 of 148 total aligned reads displayed.

AGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGTTCCGGTTGGTGTTGATGCTTTAATTGTATGTCACCGACG  >  NC_000913/4277795‑4278089
                                                                                                                                                   |                                                                                                                                                   
aGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCCGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGTAGATGAACAAAACTAAAGCGCCACAAg                                                                                                                                                   >  2:336642/1‑150 (MQ=255)
   ggCGCTGCGTGACGAACTGGACGGATGTATTGGGTGTGGCTGCCTTTCGCGCAGTGATTTCCCGTTGCGTAACCCTGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGgc                                                                                                                                                <  2:1063295/150‑1 (MQ=255)
        tGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGAGAATGCCCGTGGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCttt                                                                                                                                           <  1:1528473/150‑1 (MQ=255)
          cGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAg                                                                                                                                         <  1:407023/150‑1 (MQ=255)
          cGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGCGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCCTTGTGGCGCTTTAg                                                                                                                                         <  2:650965/150‑1 (MQ=255)
           gTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCCTTGTGGCGCTTTAgt                                                                                                                                        <  2:417649/150‑1 (MQ=255)
           gTGAAGAACTGGACTGATGTATTGGGTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAgt                                                                                                                                        <  1:1489975/150‑1 (MQ=255)
            tGACTAACTGTACGGATGTATTGGTGGTGGCTGCCTTACGAGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAgtt                                                                                                                                       <  2:879032/150‑1 (MQ=255)
            tGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCTCCACAAGGGCGCTTTAgtt                                                                                                                                       >  1:254476/1‑150 (MQ=255)
            tGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAgtt                                                                                                                                       >  2:1165038/1‑150 (MQ=255)
              ccGAACTGGAAGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGACCGTTGCGTAACCCGGGCGACCGCTTAGGAGAATAAGGTACCGGCGCACGCTTGCTGGAAGATGAACTAAACTAAAGCGCCCTTGTGGCGCTTTAgtttt                                                                                                                                     <  2:308816/149‑2 (MQ=255)
               cGAACTGGACGGATGTATTGGTGGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAgtttgt                                                                                                                                    <  1:1380490/150‑1 (MQ=255)
                gAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCCTTGTGGCGCTTTAgttttgt                                                                                                                                   <  1:916444/150‑4 (MQ=255)
                 aaCTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCCTTGTGGCGCTTTAgttttgtt                                                                                                                                  <  1:485010/150‑5 (MQ=255)
                   caggACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTATTc                                                                                                                                >  2:1683347/3‑150 (MQ=255)
                     ggtcGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCg                                                                                                                              <  1:522647/147‑1 (MQ=255)
                     ggACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCg                                                                                                                              >  2:944286/1‑150 (MQ=255)
                          gATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCcaca                                                                                                                                                     >  2:1586466/1‑122 (MQ=255)
                          gATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCcaca                                                                                                                                                     <  1:1586466/122‑1 (MQ=255)
                            tGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATGGCCCGTGGCGTAACCCGGGCGACCGCTTAGGAGAAGACGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttg                                                                                                                       <  1:535790/150‑1 (MQ=255)
                            ggTATTGGTTGTGGCTGCCTATGGCGCAGTGATTGCCCGTTGCGTAAACCGGGCGTCCGCTTAGTAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttg                                                                                                                       <  1:129359/149‑1 (MQ=255)
                             gTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCGTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttgt                                                                                                                      >  2:778025/1‑150 (MQ=255)
                              tATTGGTTGTGGCTGCCTTTCGCGCAGTGATAGCCCTTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAATGTACAGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCAGgtctttgtc                                                                                                                     >  1:238103/1‑150 (MQ=255)
                              tATTGGATGTGGCTGCCTTTCGAGCAGTGATTGCCCGTTGCGTAACCCGGGAGACCGATTAGGAGAAGAAGGTACCGCCGCACGATTGCTGGAAGATCAACAAAAAAAAAGCCCCACATGGCCAGTTTATTTTGTTTTCGGgaatttgac                                                                                                                     >  1:580994/1‑148 (MQ=255)
                               aTTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttgtct                                                                                                                    >  1:1621211/1‑150 (MQ=255)
                                 tGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAAAAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttgtcttt                                                                                                                  >  2:1236750/1‑150 (MQ=255)
                                  ggTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGCCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCTCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTtc                                                                                                                 >  2:1368785/1‑150 (MQ=255)
                                  ggTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTtc                                                                                                                 >  2:1434971/1‑150 (MQ=255)
                                    ttGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTGGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTtctc                                                                                                               <  2:1496270/150‑1 (MQ=255)
                                      gtgGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTtctctc                                                                                                             <  1:1319690/150‑1 (MQ=255)
                                        ggCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTa                                                                                                           >  2:227766/1‑150 (MQ=255)
                                            gCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGCCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCATTAGTTAGTTTTCCGGTCTTTGTCTTTCTCTCTATccc                                                                                                       >  2:1666186/1‑150 (MQ=255)
                                             ccTTTCGCGCAGTGATTGCCCGTGGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCg                                                                                                      <  1:1224226/150‑1 (MQ=255)
                                              cTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTGGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGc                                                                                                     <  2:52704/150‑1 (MQ=255)
                                              cTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGc                                                                                                     >  2:208664/1‑150 (MQ=255)
                                               tttCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCt                                                                                                    >  2:324083/1‑150 (MQ=255)
                                                    cgcAGTGATTGCCCGTTGCGGAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTac                                                                                               <  1:1366150/150‑1 (MQ=255)
                                                       aGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTAcaca                                                                                            <  1:186446/150‑1 (MQ=255)
                                                          gATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACagga                                                                                         >  1:788327/1‑150 (MQ=255)
                                                           tttGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACaggag                                                                                        <  1:814997/149‑1 (MQ=255)
                                                           aTTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACaggag                                                                                        >  2:512692/1‑150 (MQ=255)
                                                           aTTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACaggag                                                                                        <  1:59848/150‑1 (MQ=255)
                                                              gCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCTCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGt                                                                                     >  2:1100048/1‑150 (MQ=255)
                                                                 cGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTc                                                                                  >  2:127633/1‑150 (MQ=255)
                                                                 cGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTc                                                                                  <  2:504957/150‑1 (MQ=255)
                                                                    tGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTcccc                                                                               >  1:65552/1‑150 (MQ=255)
                                                                     gCGTAACCCGGGAGACCGCTTAGGAGAAGAAGGTACCTGCGCACGCTTGATGGAAGATGAACAAAACTAAAGCGCCACAATGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTccccc                                                                              <  2:350871/150‑1 (MQ=255)
                                                                      cGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCg                                                                             <  2:709459/150‑1 (MQ=255)
                                                                       gTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGa                                                                            >  2:954048/1‑150 (MQ=255)
                                                                         aaCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACg                                                                          >  2:596334/1‑150 (MQ=255)
                                                                          aCCCGGTCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTAGCTGGAAGAAGAACAAAACTAAAGCGCAACAAGGGCGCGTTAGTTTGTTTTCCGGTCTTTCTCTTTCTCTCTATCCCGCTTGTACACAGGACGGTTTCCCCCGACGt                                                                         >  1:182344/1‑150 (MQ=255)
                                                                             cGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCaa                                                                      >  2:1368338/1‑150 (MQ=255)
                                                                               ggCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAaca                                                                    <  1:954048/150‑1 (MQ=255)
                                                                                   aCCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACAcc                                                                >  1:657602/1‑150 (MQ=255)
                                                                                      gCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCa                                                             >  1:1668693/1‑150 (MQ=255)
                                                                                       cTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCAt                                                            >  1:1513005/1‑150 (MQ=255)
                                                                                          aGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCg                                                         <  2:127832/150‑1 (MQ=255)
                                                                                            gagaAGAAGGTACCGGCGCATGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACATCATTagag                                                       >  1:691801/1‑150 (MQ=255)
                                                                                            gagaAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCTCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAg                                                       <  2:254476/150‑1 (MQ=255)
                                                                                            gagaAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAg                                                       <  2:144220/150‑1 (MQ=255)
                                                                                            gagaAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAg                                                       >  1:221590/1‑150 (MQ=255)
                                                                                            gagaAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAg                                                       <  1:208664/150‑1 (MQ=255)
                                                                                              gaagaaGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCa                                                     >  1:1378194/1‑150 (MQ=255)
                                                                                              gaagaaGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCa                                                     >  1:348179/1‑150 (MQ=255)
                                                                                              ccagaagGTACCGGCGCACGCTTGCTGTAAGATGTACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGGCTTTGTCTTTCTCTCTATCCATTTTGTAAATTTTAGGGTTTCCCCCGAGGTCAACACACCTCATTCGAGCa                                                     <  2:1314175/148‑1 (MQ=255)
                                                                                                   agGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACgtgg                                                <  1:1165038/150‑1 (MQ=255)
                                                                                                    ggTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACgtggt                                               >  1:358840/1‑150 (MQ=255)
                                                                                                      tACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGtggtgg                                             >  2:1012317/1‑150 (MQ=255)
                                                                                                        ccGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAg                                           <  1:944286/150‑1 (MQ=255)
                                                                                                        ccGGCGCACGCGTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAg                                           <  2:114070/150‑1 (MQ=255)
                                                                                                          ggCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGt                                         >  1:739702/1‑150 (MQ=255)
                                                                                                             gcACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGTTcc                                      >  1:1224590/1‑150 (MQ=255)
                                                                                                              cACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGAGCTTTAGTTTGTTTACGGGTATTTGTCTTTCAGTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCCAGCACGTGGGGCAGGTTCCg                                     >  2:105391/1‑150 (MQ=255)
                                                                                                                      cTGGAAGATGAACAAAACTAAAGCGGCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGTTCCGGTTGgtgt                             <  1:227766/150‑1 (MQ=255)
                                                                                                                      cTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCACCACACCTCATTCGAGCACGTGGTGGAGGTTCCGGTTGgtgt                             >  1:1452674/1‑150 (MQ=255)
                                                                                                                      cTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGTTCCGGTTGgtgt                             <  1:1100048/150‑1 (MQ=255)
                                                                                                                      cTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGTTCCGGATGgtgt                             >  2:191109/1‑150 (MQ=255)
                                                                                                                      cTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCGTTAGTTTGTTTTCCGGGCTTTGGCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGTTCCGGTTGgtgt                             <  2:1401834/150‑1 (MQ=255)
                                                                                                                        ggAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGTTCCGGTTGGTGTTg                           <  2:394281/150‑1 (MQ=255)
                                                                                                                          aatatcaaCAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACCCAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGTTCCGGTTGGTGTTGAt                         <  1:887341/144‑1 (MQ=255)
                                                                                                                           agagGAACAACACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGGCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGACGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGGTCCGGTTGGTGTTGATg                        <  1:116534/150‑1 (MQ=255)
                                                                                                                           aGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGTTCCGGTTGGTGTTGATg                        >  2:1697388/1‑150 (MQ=255)
                                                                                                                           aGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGTTCCGGTTGGTGTTGATg                        <  1:143203/150‑1 (MQ=255)
                                                                                                                            gATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGTTCCGGTTGGTGTTGATGc                       <  1:1368338/150‑1 (MQ=255)
                                                                                                                               taaCAAAACTAAATCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGTGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGTTCCGGTTGGTGTTGATGCttt                    <  2:886300/149‑1 (MQ=255)
                                                                                                                               gAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGATGTTCCGGTTGGTGTTGATGCttt                    <  2:1585337/150‑1 (MQ=255)
                                                                                                                                 aCAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGTTCCGGTTGGTGTTGATGCTTTaa                  >  2:1497036/1‑150 (MQ=255)
                                                                                                                                 aCAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGTTCCGGTTGGTGTTGATGCTTTaa                  >  1:1136958/1‑150 (MQ=255)
                                                                                                                                 aCAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGGCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGTTCCGGTTGGTGTTGATGCTTTaa                  <  1:733520/150‑1 (MQ=255)
                                                                                                                                 aCAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTGTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGTTCCGGTTGGTGTTGATGCTTTaa                  >  1:74877/1‑150 (MQ=255)
                                                                                                                                   aaaaCTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGTTCCGGTTGGTGTTGATGCTTTAAtt                >  2:1013347/1‑150 (MQ=255)
                                                                                                                                    aaaCTAAAGCGCCCTTGTGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGTTCCGGTTGGTGTTGATGCTTTa                   >  1:869855/1‑146 (MQ=255)
                                                                                                                                      aCTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGTTCCGGTTGGTGTTGATGCTTTAATTGTa             <  1:854196/150‑1 (MQ=255)
                                                                                                                                      aCTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGAGGTTACGGGTGGTGGTGATTCTTTAATTGTAt            >  2:1289061/1‑150 (MQ=255)
                                                                                                                                           aGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGTTCCGGTTGGTGTTGATGCTTTAATTGTATGTCa        >  1:1663267/1‑150 (MQ=255)
                                                                                                                                           aGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGTTCCGGTTGGTGTTGATGCTTTAATTGTATGTCa        <  1:1423395/150‑1 (MQ=255)
                                                                                                                                              gcCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGCCGTCAACACCCCTCATTCGCGCACGTGGTGGAGGTTCCGGTTGGTGTTGATGATTTAATTGTATGTCACCg     >  1:950292/1‑150 (MQ=255)
                                                                                                                                               ccACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGTTCCGGTTGGTGTTGATGCTTTAATTGTATGTCACCgc    >  1:1133482/1‑149 (MQ=255)
                                                                                                                                               ccACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGTTCCGGTTGGTGTTGATGCTTTAATTGTATGTCACCGa    <  2:502104/150‑1 (MQ=255)
                                                                                                                                                cacaAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGTTCCGGTTGGTGTTGATGCTTTAATTGTATGTCACCGAc   >  2:1405618/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGGAGGGTTTCCCCCGACGTCAACACACCTCATTCGAGCACGTGGTGGAGGTTCCGGTTGGTGTTGATGCTTTAATTGTATGTCACCGACG  >  NC_000913/4277795‑4278089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: