Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,280,219 A→C 6.3% E80D (GAA→GAC yjcE → putative cation/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,280,2190AC 6.3% 296.5 / 9.1 112E80D (GAA→GACyjcEputative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (49/53);  new base C (5/2);  total (54/58)
Fisher's exact test for biased strand distribution p-value = 2.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.73e-01

Only 100 of 113 total aligned reads displayed.

CGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGC  >  NC_000913/4280071‑4280368
                                                                                                                                                    |                                                                                                                                                     
cgcttATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAAc                                                                                                                                                      <  1:738785/150‑1 (MQ=255)
    tATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATgg                                                                                                                                                  >  1:1527473/1‑150 (MQ=255)
    tATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATgg                                                                                                                                                  >  2:1024187/1‑150 (MQ=255)
    tATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTGTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATgg                                                                                                                                                  >  1:1311127/1‑150 (MQ=255)
             cGCCATCGGTGCGCTACTGGCGTGGCCGACGGTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGAt                                                                                                                                         <  2:1145611/150‑1 (MQ=255)
              gCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGAtt                                                                                                                                        >  2:658576/1‑150 (MQ=255)
               ccATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGAttt                                                                                                                                       >  2:728812/1‑150 (MQ=255)
                cATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGAtttt                                                                                                                                      >  2:369301/1‑150 (MQ=255)
                cATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCAGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCGCGAACATGGTCGAGAGAtttt                                                                                                                                      >  1:1707463/1‑150 (MQ=255)
                   cGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCgg                                                                                                                                   >  2:177879/1‑150 (MQ=255)
                    ggTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGc                                                                                                                                  <  2:518713/150‑1 (MQ=255)
                          cTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTcgcg                                                                                                                            >  1:645136/1‑150 (MQ=255)
                          cTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTcgcg                                                                                                                            >  2:1354585/1‑150 (MQ=255)
                          cTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTcgcg                                                                                                                            >  2:729742/1‑150 (MQ=255)
                           tACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCGTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCgcgc                                                                                                                           <  1:897420/150‑1 (MQ=255)
                           tACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCgcgc                                                                                                                           <  2:362089/150‑1 (MQ=255)
                           tACTGGCGGGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCgcgc                                                                                                                           <  1:1714570/150‑1 (MQ=255)
                           tACTGGCGGGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCgcgc                                                                                                                           <  1:141846/150‑1 (MQ=255)
                            aCTGGCGTGGCCGACGTTTGGTTTGCATGGGGAGTTTGATCCTGATCTCTTTTTAGTCTGGGTTATCCCGCCGTTGCTGTGCGGTGATGGCTGGAAAACGCCGCCCCGTGAATTTCTCGAACTTGGTCGAGAGATTTTCGGCCTCgcgct                                                                                                                          <  1:438049/150‑1 (MQ=255)
                              tGGGGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCgcgctgg                                                                                                                        <  1:759984/150‑1 (MQ=255)
                                   tGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCgcgctggcgctg                                                                                                                   >  1:512697/1‑150 (MQ=255)
                                   gggCCGACGTTTGGTTTGCATGTGGAGTTTGATCCCGAACTCTTTTTAGGCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCgcgctggcgctg                                                                                                                   <  2:1015493/149‑1 (MQ=255)
                                    ggCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGcgctggcgctgg                                                                                                                  >  1:1466428/1‑150 (MQ=255)
                                     gCCGACGGTGGGTTTGCATGTGGAGGTTGATCCTCAACTCTTTTTAGTCTTGGTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCTGCCTCGCGCTGGCGCtggt                                                                                                                 <  1:1519630/150‑1 (MQ=255)
                                      ccGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCAGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCtggtg                                                                                                                >  1:824964/1‑150 (MQ=255)
                                      ccGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGATGTTCGCTGATGGCTGGAAAACGCCGACACGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCtggtg                                                                                                                >  1:1234228/1‑150 (MQ=255)
                                       cGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCtggtgg                                                                                                               >  2:1520662/1‑150 (MQ=255)
                                               ggtttgCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCAcc                                                                                                       <  1:1222545/150‑1 (MQ=255)
                                                gtttgCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCg                                                                                                      >  2:572024/1‑150 (MQ=255)
                                                   tgCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTgg                                                                                                   >  2:1103157/1‑150 (MQ=255)
                                                     cATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGATGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTAGCGCTGGAGCTGGTGGTGGTCACCGTGGTc                                                                                                 >  2:334323/1‑150 (MQ=255)
                                                       tgtgGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCTACCCGTGAATTTCTCGAACATGGTAGAGAGATTTTCGGCCTCGCGCTGGCGCTTGTGGTGGTCACCGTGGTCgg                                                                                               >  2:1107250/1‑150 (MQ=255)
                                                        gaggAGTTTGATCCTGAACTCTTTTTAGTCTTCTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACACGTGAATAACTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGCTGGTGGTCACCGTGGTCGGc                                                                                              >  1:745382/3‑150 (MQ=255)
                                                           gAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATTGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGCTCACCGTGGTCGGCAtt                                                                                           >  1:865420/1‑150 (MQ=255)
                                                           gAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCAtt                                                                                           <  1:1350977/150‑1 (MQ=255)
                                                             gTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTTCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTgg                                                                                         >  1:1177556/1‑150 (MQ=255)
                                                             gTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTgg                                                                                         <  2:706123/150‑1 (MQ=255)
                                                             gTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTgg                                                                                         <  1:997554/150‑1 (MQ=255)
                                                               ttGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCt                                                                                       >  1:1443606/1‑150 (MQ=255)
                                                               ttGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCt                                                                                       >  2:53052/1‑150 (MQ=255)
                                                                 gATCCTGCACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACACGTGAATTTCTCGAACATGGTCGAGAGCTTTTCGGCCTCGCGGTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTc                                                                                     >  2:1149862/1‑150 (MQ=255)
                                                                 gATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGAATTTCGGCCTCGCGCGGGCGCTGGTGGTGGTCACCGTGGTCGGGATTGGCttg                                                                                     >  2:1144005/1‑149 (MQ=255)
                                                                  aTCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTTGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTcc                                                                                    <  1:970899/150‑1 (MQ=255)
                                                                  aTCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTcc                                                                                    >  1:1349728/1‑150 (MQ=255)
                                                                   tCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCt                                                                                   >  2:1240572/1‑150 (MQ=255)
                                                                   tCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCt                                                                                   <  1:1219119/150‑1 (MQ=255)
                                                                        aaCTCTTTTTAGTCTTGTATCTCCCGCCGTCGCAGTGCGCTGATGTCTGGAAAACGACGACCCGTGTATTTCTCGAACTTGGTCGAGAGATTTTCGGCCTCGAGCTGGCGCTGGTGGTGGTCAGCGTGGTCGGCATTGGCTTCCTTAttt                                                                              <  2:56017/150‑1 (MQ=255)
                                                                            ctTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTg                                                                          <  2:482725/150‑1 (MQ=255)
                                                                                ttAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGggtg                                                                      <  2:1408651/150‑1 (MQ=255)
                                                                                 tAGTCGTGATTATCCCGCCGTTGATGTTCGCTGAAGGCTGGATCACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGGTTTGGCTTCCTTATTTACTGggtgg                                                                     <  2:1068309/150‑1 (MQ=255)
                                                                                  aGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGggtggt                                                                    <  2:540324/150‑1 (MQ=255)
                                                                                   gTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGgtggtg                                                                   >  2:518746/1‑150 (MQ=255)
                                                                                   gTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGgtggtg                                                                   <  1:729742/150‑1 (MQ=255)
                                                                                     cTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGcc                                                                 >  1:1491995/1‑150 (MQ=255)
                                                                                     cTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGcc                                                                 >  1:1162967/1‑150 (MQ=255)
                                                                                        ttttATCCCGCCGTAGCTGTTCGCTTATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCTGCATTGGCTTCCTTATTTACTGGGTGGTGCCggg                                                              <  1:362134/149‑1 (MQ=255)
                                                                                        gTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGGCCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCTGCCTCGCGCGGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCggg                                                              >  2:1401806/1‑150 (MQ=255)
                                                                                        gTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCggg                                                              >  1:842347/1‑150 (MQ=255)
                                                                                        gTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCggg                                                              >  1:425496/1‑150 (MQ=255)
                                                                                        gTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGGCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCggg                                                              <  1:1520662/150‑1 (MQ=255)
                                                                                         tttATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGc                                                             <  1:990324/150‑1 (MQ=255)
                                                                                         tttATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGc                                                             <  1:740214/150‑1 (MQ=255)
                                                                                           tATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTGCTGGGTGGTGCCGGGCAt                                                           <  1:1175069/150‑1 (MQ=255)
                                                                                             tCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTc                                                         <  1:177879/150‑1 (MQ=255)
                                                                                              cccGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTcc                                                        <  2:1482044/150‑1 (MQ=255)
                                                                                                 gccgTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCt                                                     <  1:2722/150‑1 (MQ=255)
                                                                                                 gccgTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCt                                                     >  1:1278103/1‑150 (MQ=255)
                                                                                                   cgTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGa                                                   <  1:371131/150‑1 (MQ=255)
                                                                                                     ttGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATc                                                 <  1:971197/150‑1 (MQ=255)
                                                                                                     ttGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCGGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATc                                                 <  1:685313/150‑1 (MQ=255)
                                                                                                      tGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGCTCGAGAGATTTTCGGCCTCTCGCTGGCGCTGGTGGAGGTCACCGTGGTGGGGTGTGGCGTCCTTTGTTACTGGGTGGTGCCGGGCATTCCGCTGATcc                                                >  2:337113/1‑150 (MQ=255)
                                                                                                      ggCTGTTCGCTGATGGCTGGAAAACGCCGCCCCGTGAATTTCTCGAACATGGGCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATcc                                                <  1:719227/149‑1 (MQ=255)
                                                                                                          gTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCTCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGc                                            >  2:204678/1‑150 (MQ=255)
                                                                                                          gTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGc                                            <  2:1466428/150‑1 (MQ=255)
                                                                                                          gTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGc                                            <  1:1119674/150‑1 (MQ=255)
                                                                                                             cGCTGAGGGCTGGACAACGCCGACCCGTTAATTTCTCGATCATGGGCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCTCCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCtt                                         <  1:122124/150‑1 (MQ=255)
                                                                                                               cTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTg                                       <  1:1194991/150‑1 (MQ=255)
                                                                                                                   tGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCt                                   >  1:1621939/1‑150 (MQ=255)
                                                                                                                     gCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTgg                                 >  1:1675394/1‑150 (MQ=255)
                                                                                                                      cTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTggc                                >  1:248651/1‑150 (MQ=255)
                                                                                                                            aaCGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGt                          <  1:835157/150‑1 (MQ=255)
                                                                                                                            aaCGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGt                          <  1:1021389/150‑1 (MQ=255)
                                                                                                                             ccGCCGACCCGTGACTTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGGCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTg                         <  1:1213690/149‑1 (MQ=255)
                                                                                                                             ccGCCGACCCGTGAATTTCTCGATCATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGGCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTg                         <  2:1604716/149‑1 (MQ=255)
                                                                                                                               gCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCt                       <  2:16820/150‑1 (MQ=255)
                                                                                                                                ccGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCtt                      <  1:960843/150‑1 (MQ=255)
                                                                                                                                  gACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGGCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTtc                    <  2:1386573/150‑1 (MQ=255)
                                                                                                                                    cccGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGGCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTtctc                  <  2:807605/150‑1 (MQ=255)
                                                                                                                                      cGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTccg                <  2:842347/150‑1 (MQ=255)
                                                                                                                                      cGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTccg                <  2:425496/150‑1 (MQ=255)
                                                                                                                                         gAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCGGGCGCTGGTGGTGGTCACCGTGGTCGGCATAGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTccgacc             <  2:1198776/150‑1 (MQ=255)
                                                                                                                                          aaTCTCTCGACCATGTTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTccgaccg            >  1:800261/1‑150 (MQ=255)
                                                                                                                                          aaTCTCTCGACCATGTTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTccgaccg            >  1:1655514/1‑150 (MQ=255)
                                                                                                                                           atctctcGACCATGTTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTccgaccga           >  1:721465/4‑150 (MQ=255)
                                                                                                                                              tctcGACCATGTTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCtt                      <  2:1141524/136‑1 (MQ=255)
                                                                                                                                              tctcGACCATGTTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCtt                      >  1:1141524/1‑136 (MQ=255)
                                                                                                                                               ctcGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCt       >  2:515055/1‑150 (MQ=255)
                                                                                                                                                tcGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCtg      >  1:1722783/1‑150 (MQ=255)
                                                                                                                                                 cGACCATGTTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCtgt     >  1:1500834/1‑150 (MQ=255)
                                                                                                                                                    aCATGGTCGAGAGATTTTCGGCCTCGCGCGGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTAGTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGgc  <  1:695869/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGCTTATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGC  >  NC_000913/4280071‑4280368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: